ID | 48565 |
JaLCDOI | |
フルテキストURL | |
著者 |
Zhang, Kai
Department of Bacteriology, Okayama University Graduate School of Medicine
Yamamoto, Yumiko
Department of Bacteriology, Okayama University Graduate School of Medicine
Kaken ID
publons
researchmap
Suzuki, Tomonori
Department of Bacteriology, Okayama University Graduate School of Medicine
Kaken ID
publons
researchmap
Yokota, Kenji
Graduate School of Health Sciences, Okayama University
ORCID
Kaken ID
publons
researchmap
Ma, Shaobo
Department of Bacteriology, Okayama University Graduate School of Medicine
Nengah Dwi Fatmawati, Ni
Department of Bacteriology, Okayama University Graduate School of Medicine
Oguma, Keiji
Department of Bacteriology, Okayama University Graduate School of Medicine
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抄録 | Cultured Clostridium botulinum strains produce progenitor toxins designated as 12S, 16S, and 19S toxins. The 12S toxin consists of a neurotoxin (NTX, 7S) and a non-toxic non-hemagglutinin (NTNH). The 16S and 19S toxins are formed by conjugation of the 12S toxin with hemagglutinin (HA), and the 19S toxin is a dimer of the 16S toxin. Type A cultures produce all 3 of these progenitor toxins, while type E produces only the 12S toxin. The 7S toxin is cleaved into heavy (H) and light (L) chains by a protease(s) in some strains, and the H chain has 2 domains, the N-terminus (Hn) and C-terminus (Hc). It has been reported that type A toxins bind to the intestinal cells or cultured cells via either HA or Hc. In this study, we investigated the binding of type A and E toxins to Caco-2 cells using Western blot analysis. Both the type E 7S and 12S toxins bound to the cells, with the 7S toxin binding more strongly, whereas, in the type A strain, only the 16S/19S toxins showed obvious binding. Pre-incubation of the type E 7S toxin with IgG against recombinant type E Hc significantly inhibited the 7S toxin binding, indicating that Hc might be a main binding domain of the type E toxin.
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キーワード | Clostridum botulinum
neurotoxins
Caco-2
binding
Hc
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Amo Type | Original Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2012-06
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巻 | 66巻
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号 | 3号
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出版者 | Okayama University Medical School
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開始ページ | 253
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終了ページ | 261
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | CopyrightⒸ 2012 by Okayama University Medical School
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT | |
関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/48451
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