ID | 65944 |
フルテキストURL | |
著者 |
Saito, Yukihiro
Department of Cardiovascular Medicine, Okayama University Hospital
Nose, Naoko
Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Iida, Toshihiro
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Akazawa, Kaoru
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kanno, Takayuki
Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Fujimoto, Yuki
Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Sasaki, Takanori
Okayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Akehi, Masaru
Okayama Medical Innovation Center, Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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Higuchi, Takahiro
Molecular Imaging Project of RECTOR Program, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Akagi, Satoshi
Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
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Yoshida, Masashi
Department of Chronic Kidney Disease and Cardiovascular Disease, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Miyoshi, Toru
Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
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Ito, Hiroshi
Department of General Internal Medicine 3, Kawasaki Medical School
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Nakamura, Kazufumi
Department of Cardiovascular Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | Introduction: Transplantation of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is a promising treatment for heart failure. Information on long-term cell engraftment after transplantation is clinically important. However, clinically applicable evaluation methods have not yet been established.
Methods: In this study, to noninvasively assess transplanted cell engraftment, human SLC5A5, which encodes a sodium/iodide symporter (NIS) that transports radioactive tracers such as 125I, 18F-tetrafluoroborate (TFB), and 99mTc-pertechnetate (99mTcO4−), was transduced into human induced pluripotent stem cells (iPSCs), and nuclear medicine imaging was used to track engrafted human iPSC-CMs. Results: To evaluate the pluripotency of NIS-expressing human iPSCs, they were subcutaneously transplanted into immunodeficient rats. Teratomas were detected by 99mTcO4− single photon emission computed tomography (SPECT/CT) imaging. NIS expression and the uptake ability of 125I were maintained in purified human iPSC-CMs. NIS-expressing human iPSC-CMs transplanted into immunodeficient rats could be detected over time using 99mTcO4− SPECT/CT imaging. Unexpectedly, NIS expression affected cell proliferation of human iPSCs and iPSC-derived cells. Discussion: Such functionally designed iPSC-CMs have potential clinical applications as a noninvasive method of grafted cell evaluation, but further studies are needed to determine the effects of NIS transduction on cellular characteristics and functions. |
キーワード | sodium/iodide symporter
human induced pluripotent stem cell-derived cardiomyocytes
single photon emission computed tomography
cell-based therapy
in vivo imaging
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発行日 | 2023-09-07
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出版物タイトル |
Frontiers in Cardiovascular Medicine
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巻 | 10巻
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出版者 | Frontiers Media
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開始ページ | 1261330
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ISSN | 2297-055X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2023 Saito, Nose, Iida, Akazawa, Kanno, Fujimoto, Sasaki, Akehi, Higuchi, Akagi, Yoshida, Miyoshi, Ito and Nakamura.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3389/fcvm.2023.1261330
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Saito Y, Nose N, Iida T, Akazawa K, Kanno T, Fujimoto Y, Sasaki T, Akehi M, Higuchi T, Akagi S, Yoshida M, Miyoshi T, Ito H and Nakamura K (2023) In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging. Front. Cardiovasc. Med. 10:1261330. doi: 10.3389/fcvm.2023.1261330
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助成機関名 |
Japan Society for the Promotion of Science
Uehara Memorial Foundation
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助成番号 | 23K07508
FY2021
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