ID | 62284 |
フルテキストURL | |
著者 |
Liu, Shihui
Department of Ophthalmology, Okayama University Graduate School of Interdisciplinary Science and Engineering in Health Systems
Matsuo, Toshihiko
Department of Ophthalmology, Okayama University Graduate School of Interdisciplinary Science and Engineering in Health Systems
ORCID
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Miyaji, Mary
Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Hosoya, Osamu
Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | The present study aimed to evaluate the effects of NK-4 on the apoptosis of photoreceptors in a rat model of retinitis pigmentosa and explore the mechanism underlying anti-apoptosis activity. The Royal College of Surgeons (RCS) rats received an intravitreous injection of NK-4 solution in the left eye and vehicle control in the right eye. Apoptosis was detected by TUNEL method in frozen sections of the eyes. The retinal tissues of the rats were dissected for RNA-seq analysis. Functional and pathway enrichment analyses of differentially expressed genes (DEGs) were performed by using Metascape and DAVID software. The expression levels of DEGs were confirmed by real-time quantitative PCR (RT-qPCR). The number of apoptotic cells decreased in the outer nuclear layer (ONL) and the thickness of the ONL was significantly thicker in the retina of NK-4-injected eyes, compared with control eyes. Five DEGs were identified by RNA-seq analysis, and Hmox1, Mt1, Atf5, Slc7a11, and Bdh2 were confirmed to be up-regulated by RT-qPCR. Functional and pathway enrichment analysis of the up-regulated genes showed that anti-apoptosis effects of NK-4 in the retina of RCS rats may be related to the pathways of metal ion homeostasis, negative regulation of neuron death, response to toxic substance, and pigment metabolic process. We found a potential mechanism of NK-4, providing a new viewpoint for the development of more therapeutic uses of NK-4 in the future.
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キーワード | NK-4
retina
apoptosis
RNA-seq
photoreceptor
retinitis pigmentosa
antioxidant
metabolism
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発行日 | 2021-7-19
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出版物タイトル |
Pharmaceuticals
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巻 | 14巻
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号 | 7号
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出版者 | MDPI AG
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開始ページ | 694
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ISSN | 1424-8247
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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論文のバージョン | publisher
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DOI | |
関連URL | isVersionOf https://doi.org/10.3390/ph14070694
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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助成機関名 |
Science and Technology Promotion grants in Okayama Prefecture, Japan
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助成番号 | 2018–2020
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オープンアクセス(出版社) |
OA
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