| JaLCDOI | 10.18926/AMO/46626 |
|---|---|
| フルテキストURL | 65_3_151.pdf |
| 著者 | Fujiwara, Toshiyoshi| |
| 抄録 | Replication-selective tumor-specific viruses constitute a novel approach for treatment of neoplastic disease. These vectors are designed to induce virus-mediated lysis of tumor cells after selective viral propagation within the tumor. Human telomerase is highly active in more than 85ｵ of primary cancers, regardless of their tissue origins, and its activity correlates closely with human telomerase reverse transcriptase (hTERT) expression. We constructed an attenuated adenovirus 5 vector (Telomelysin, OBP-301), in which the hTERT promoter element drives expression of E1 genes. Since only tumor cells that express telomerase activity would activate this promoter, the hTERT proximal promoter would allow for preferential expression of viral genes in tumor cells, leading to selective viral replication and oncolytic cell death. Lymphatic invasion is a major route for cancer cell dissemination, and adequate treatment of locoregional lymph nodes is required for curative treatment in patients with gastrointestinal tumors. We demonstrated that intratumoral injection of Telomelysin mediates effective in vivo purging of metastatic tumor cells from regional lymph nodes. Moreover, using noninvasive whole-body imaging, we found that intratumoral injection of Telomelysin followed by regional irradiation induces a substantial antitumor effect, resulting from tumor cell-specific radiosensitization, in an orthotopic human esophageal cancer xenograft model. These results illustrate the potential of oncolytic virotherapy as a promising strategy in the management of human gastrointestinal cancer. |
| キーワード | telomerase adenovirus metastasis lymph node colorectal cancer |
| Amo Type | Review |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2011-06 |
| 巻 | 65巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 151 |
| 終了ページ | 162 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2011 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 21709712 |
| Web of Science KeyUT | 000292017500001 |
| 著者 | 野間 和広| 田中屋 宏爾| 竹内 仁司| 小長 英二| 藤原 俊義| |
|---|---|
| 発行日 | 2011-04-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 藤原 俊義| |
|---|---|
| 発行日 | 2010-12-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 122巻 |
| 号 | 3号 |
| 資料タイプ | その他 |
| 著者 | 藤原 俊義| |
|---|---|
| 発行日 | 2010-12-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 122巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 児島 亨| 橋本 悠里| 香川 俊輔| 田中 紀章| 浦田 泰生| 藤原 俊義| |
|---|---|
| 発行日 | 2010-12-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 122巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 永坂 岳司| 田中 紀章| 孫 冬生| 猶本 良夫| 松原 長秀| 八木 孝仁| 藤原 俊義| |
|---|---|
| 発行日 | 2010-08-02 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 122巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Teraishi, Fuminori| Kagawa, Shunsuke| Watanabe, Takanori| Tango, Yasuhisa| Kawashima, Takeshi| Umeoka, Tatsuo| Nisizaki, Masahiko| Tanaka, Noriaki| Fujiwara, Toshiyoshi| |
|---|---|
| 発行日 | 2005-08-01 |
| 出版物タイトル | FEBS Letters |
| 巻 | 579巻 |
| 号 | 19号 |
| 資料タイプ | 学術雑誌論文 |
| JaLCDOI | 10.18926/AMO/31570 |
|---|---|
| フルテキストURL | fulltext.pdf |
| 著者 | Matsuoka, Junji| Sakagami, Kenichi| Fujiwara, Toshiyoshi| Onoda, Tadashi| Idani, Hitoshi| Gochi, Akira| Orita, Kunzo| |
| 抄録 | A sustained release system for interleukin-2 (IL-2), and IL-2 mini-pellet (IL-2 mp), was developed by fusing IL-2 into a needle shaped collagen. Serum concentration of IL-2 after a single subcutaneous injection of the IL-2 mp into C57BL/6 mice remained elevated longer than after an injection of aqueous IL-2. IL-2 in the serum became undetectable by 6h after a subcutaneous injection of 1 x 10(6) unit of IL-2 in phosphate-buffered saline (PBS). In contrast, after a single subcutaneous injection of IL-2 mp containing the same amount of IL-2, the concentration of IL-2 increased to its maximum at 6h after injection, then began to decrease gradually. IL-2 was detected even on the third day after a single subcutaneous injection of one IL-2 mp. Augmentation of NK activity and generation of IL-2 activated killer cells were observed in the spleen from day 1--day 3 after a single subcutaneous injection of IL-2 mp into C57BL/6 mice. This activation was not observed following a single subcutaneous injection of the same amount of IL-2 in PBS. Adoptive immunotherapy by a single subcutaneous injection of IL-2 mp followed by intravenous injections of in vitro cultured IL-2 activated killer cells showed better results in decreasing the number of metastases of Lewis lung carcinoma in C57BL/6 mice than immunotherapy using IL-2 solution.(ABSTRACT TRUNCATED AT 250 WORDS) |
| キーワード | IL-2 drug delivery system immunotherapy mouse |
| Amo Type | Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 1993-04 |
| 巻 | 47巻 |
| 号 | 2号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 79 |
| 終了ページ | 84 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 8506753 |
| Web of Science KeyUT | A1993LA45200002 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Umeoka, Tatsuo| Kawashima, Takeshi| Kagawa, Shunsuke| Teraishi, Fuminori| Taki, Masaki| Nishizaki, Masahiko| Kyo, Satoru| Nagai, Katsuyuki| Urata, Yasuo| Tanaka, Noriaki| Fujiwara, Toshiyoshi| |
| キーワード | GFP adenovirus telomerase replication gene therapy |
| 備考 | Published with permission from the copyright holder. This is the author's copy, as published in Cancer Research, September 2004, Volume 64, Issue 17, Pages 6259-6265. Copyright © 2004 American Association for Cancer Research. All rights reserved.| |
| 発行日 | 2004-09-01 |
| 出版物タイトル | Cancer Research |
| 巻 | 64巻 |
| 号 | 17号 |
| 出版者 | American Association for Cancer Research |
| 開始ページ | 6259 |
| 終了ページ | 6265 |
| ISSN | 0008-5472 |
| NCID | AA00598557 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | American Association for Cancer Research |
| 論文のバージョン | author |
| PubMed ID | 15342413 |
| DOI | 10.1158/0008-5472.can-04-1335 |
| Web of Science KeyUT | 000223603200048 |
| オフィシャル URL | http://cancerres.aacrjournals.org/content/64/17/6259| |
| 関連URL | isVersionOf https://doi.org/10.1158/0008-5472.can-04-1335 |
| 著者 | 藤原 俊義| 田中 紀章| |
|---|---|
| 発行日 | 2008-12-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 120巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 遠藤 芳克| 酒井 亮| 大内 正明| 鬼松 秀樹| 日置 勝義| 香川 俊輔| 宇野 太| 渡邉 雄一| 浦田 泰生| 田中 紀章| 藤原 俊義| |
|---|---|
| 発行日 | 2008-12-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 120巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| タイトル（別表記） | Two cases of acute pulmonary embolism after esophageal cancer operation |
|---|---|
| フルテキストURL | 118_41.pdf |
| 著者 | 金澤 卓| 渡辺 信之| 猶本 良夫| 小林 正彦| 白川 靖博| 山辻 知樹| 小林 直哉| 藤原 俊義| 松原 長秀| 岩垣 博巳| 松岡 順治| 田中 紀章| |
| 抄録 | We encountered two cases of pulmonary embolism (PE)/venous thrombs (DVT) after surgery for esophageal cancer. The first case was a 58-year-old male. He underwent a subtotal esophagectomy and reconstruction using the stomach via the subcutaneus route. His postoperative course was stable; however, 13 days after the operation､ he had an elevated fever and pulmonary thrombi were observed on CT-scan screening. Scintigraphy confirmed the thrombi. An inferior vena cava filter (IVCF) was inserted and anti-coagulation therapy using heparin was started. His fever subsided, and 35 days after the operation, the thrombi disappeared. He was discharged thereafter and is being followed up using warfarin. The second case was a 74-year-old male. He also underwent subtotal esophagectomy and reconstruction using the stomach via the subcutaneus route. A central line was inserted via right femoral vein; however, the artery was injured and pressurization to the vessel was needed. His post-operative course was stable and symptom-free; however, ultrasound screening detected DVT in his right femoral vein. IVCF was mserted and anti-coagulation therapy was begun, thrombi disappeared 8 days after the therapy. |
| キーワード | 食道癌 (esophageal cancer) 肺塞栓症 (pulmonary embolism) 深部静脈血栓症 (deep venous thrombs) |
| 出版物タイトル | 岡山医学会雑誌 |
| 発行日 | 2006-05-01 |
| 巻 | 118巻 |
| 号 | 1号 |
| 開始ページ | 41 |
| 終了ページ | 45 |
| ISSN | 0030-1558 |
| 言語 | 日本語 |
| 著作権者 | Copyright© 岡山医学会 |
| 論文のバージョン | publisher |
| DOI | 10.4044/joma1947.118.1_41 |
| NAID | 10017417131 |
| フルテキストURL | 118_9.pdf |
|---|---|
| 著者 | 梅岡 達生| 川嶋 健| 香川 俊輔| 寺石 文則| 滝 正樹| 京 哲| 永井 勝幸| 浦田 泰生| 田中 紀章| 藤原 俊義| |
| キーワード | GFP アデノウイルス テロメラーゼ 増幅 遺伝子治療 |
| 出版物タイトル | 岡山医学会雑誌 |
| 発行日 | 2006-05-01 |
| 巻 | 118巻 |
| 号 | 1号 |
| 開始ページ | 9 |
| 終了ページ | 15 |
| ISSN | 0030-1558 |
| 言語 | 日本語 |
| 著作権者 | Copyright© 岡山医学会 |
| 論文のバージョン | publisher |
| DOI | 10.4044/joma1947.118.1_9 |
| NAID | 10017417061 |
| タイトル（別表記） | Adenoviral p53 gene therapy for lung cancer |
|---|---|
| フルテキストURL | 119_229.pdf |
| 著者 | 藤原 俊義| 田中 紀章| |
| 抄録 | To determine the feasibility, safety, humoral immune response, and biological activity of multiple intratumoral injections of Ad5CMV-p53, and to characterize the pharmacokinetics of Ad5CMV-p53 in patients with advanced non-small cell lung cancer (NSCLC). Fifteen patients with histologically confirmed NSCLC and p53 mutations were enrolled into this phase I trial. Nine patients received escalating dose levels of Ad5CMV-p53 (1 × 109 to 1 × 1011 plaque-forming units[PFU]) as monotherapy once every 4 weeks. Six patients were treated on a 28-day schedule with Ad5CMV-p53 in combination with intravenous administration of cisplatin (80 mg/m2). Patients were monitored for toxicity, vector distribution, antibody formation, and tumor response. Fifteen patients received a total of 63 intratumoral injections of Ad5CMV-p53 without dose-limiting toxicity. The most common treatment-related toxicity was a transient fever. Specific p53 transgene expression was detected using reverse-transcriptase polymerase chain reaction in biopsied tumor tissues throughout the period of treatment despite of the presence of neutralizing anti-adenovirus antibody. Distribution studies revealed that the vector was detected in the gargle and plasma, but rarely in the urine. Thirteen of 15 patients were assessable for efficacy; one patient had a partial response (squamous cell carcinoma at the carina), 10 patients had stable disease, with three lasting ≥9 months, and 2 patients had progressive disease. Multiple courses of intratumoral Ad5CMV-p53 injection alone or in combination with intravenous administration of cisplatin were feasible and well tolerated in advanced NSCLC patients, and appeared to provide clinical benefit. |
| キーワード | p53 遺伝子 アデノウイルスベクター (adenovirus vector) 肺癌 (lung cancer) 臨床試験 (clinical trial) |
| 出版物タイトル | 岡山医学会雑誌 |
| 発行日 | 2008-01-04 |
| 巻 | 119巻 |
| 号 | 3号 |
| 開始ページ | 229 |
| 終了ページ | 234 |
| ISSN | 00301558 |
| 言語 | 日本語 |
| 著作権者 | 岡山医学会 |
| 論文のバージョン | publisher |
| DOI | 10.4044/joma.119.229 |
| NAID | 10020282723 |
| タイトル（別表記） | In vivo imaging of lymph node metastasis with telomerase-specific replication-selective adenovirus |
|---|---|
| フルテキストURL | 120_01_013_021.pdf |
| 著者 | 岸本 浩行| 児島 亨| 渡邉 雄一| 香川 俊輔| 藤原 俊哉| 宇野 太| 寺石 文則| 京 哲| 水口 裕之| 橋本 悠里| 浦田 泰生| 田中 紀章| 藤原 俊義| |
| キーワード | GFP アデノウイルス ヒトテロメラーゼ逆転写酵素 |
| 出版物タイトル | 岡山医学会雑誌 |
| 発行日 | 2008-05-01 |
| 巻 | 120巻 |
| 号 | 1号 |
| 開始ページ | 13 |
| 終了ページ | 21 |
| ISSN | 00301558 |
| 言語 | 日本語 |
| 著作権者 | 岡山医学会 |
| 論文のバージョン | publisher |
| DOI | 10.4044/joma.120.13 |
| NAID | 10024170197 |
| フルテキストURL | K000876.pdf |
|---|---|
| 著者 | 藤原 俊義| |
| 発行日 | 1990-09-30 |
| 資料タイプ | 学位論文 |
| 学位授与番号 | 甲第876号 |
| 学位授与年月日 | 1990-09-30 |
| 学位・専攻分野 | 博士（医学） |
| 授与大学 | 岡山大学 |
| 言語 | 日本語 |