ID | 63960 |
フルテキストURL | |
著者 |
Naka, Shuhei
Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
Matsuoka, Daiki
Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Goto, Kana
Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Misaki, Taro
Division of Nephrology, Seirei Hamamatsu General Hospital
Nagasawa, Yasuyuki
Department of General Internal Medicine, Hyogo College of Medicine
Ito, Seigo
Department of Internal Medicine, Japan Self-Defense Iruma Hospital
Nomura, Ryota
Department of Pediatric Dentistry, Division of Oral infection and Disease Control, Osaka University Graduate School of Dentistry
Nakano, Kazuhiko
Department of Pediatric Dentistry, Division of Oral infection and Disease Control, Osaka University Graduate School of Dentistry
Matsumoto-Nakano, Michiyo
Department of Pediatric Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
|
抄録 | Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries. The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown. In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm. Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure. Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated. The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides. Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains. Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
|
キーワード | Streptococcus mutans
collagen-binding protein
membrane permeability
cell structure
RNA-seq
|
発行日 | 2022-09-13
|
出版物タイトル |
Frontiers In Cellular And Infection Microbiology
|
巻 | 12巻
|
出版者 | Frontiers Media S.A.
|
開始ページ | 994014
|
ISSN | 2235-2988
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
OAI-PMH Set |
岡山大学
|
著作権者 | © 2022 Naka, Matsuoka, Goto, Misaki, Nagasawa, Ito, Nomura, Nakano and Matsumoto-Nakano.
|
論文のバージョン | publisher
|
PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3389/fcimb.2022.994014
|
ライセンス | https://creativecommons.org/licenses/by/4.0/
|
助成機関名 |
Japan Society for the Promotion of Science
|
助成番号 | JP17K09721
JP18H03010
JP19K10098
JP20K10225
JP21K08242
|