ID | 55316 |
フルテキストURL | |
著者 |
Takatani, Shogo
Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
Ozawa, Shinichiro
Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
Yagi, Noriyoshi
Graduate School of Biological Science, Nara Institute of Science and Technology
Hotta, Takashi
Graduate School of Biological Science, Nara Institute of Science and Technology
Hashimoto, Takashi
Graduate School of Biological Science, Nara Institute of Science and Technology
Takahashi, Yuichiro
Graduate School of Natural Science and Technology/Faculty of Science, Okayama University
ORCID
Kaken ID
publons
researchmap
Takahashi, Taku
Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
researchmap
Motose, Hiroyasu
Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
researchmap
|
抄録 | Plant cortical microtubules align perpendicular to the growth axis to determine the direction of cell growth. However, it remains unclear how plant cells form well-organized cortical microtubule arrays in the absence of a centrosome. In this study, we investigated the functions of Arabidopsis NIMA-related kinase 6 (NEK6), which regulates microtubule organization during anisotropic cell expansion. Quantitative analysis of hypocotyl cell growth in the nek6-1 mutant demonstrated that NEK6 suppresses ectopic outgrowth and promotes cell elongation in different regions of the hypocotyl. Loss of NEK6 function led to excessive microtubule waving and distortion, implying that NEK6 suppresses the aberrant cortical microtubules. Live cell imaging showed that NEK6 localizes to the microtubule lattice and to the shrinking plus and minus ends of microtubules. In agreement with this observation, the induced overexpression of NEK6 reduced and disorganized cortical microtubules and suppressed cell elongation. Furthermore, we identified five phosphorylation sites in β-tubulin that serve as substrates for NEK6 in vitro. Alanine substitution of the phosphorylation site Thr166 promoted incorporation of mutant β-tubulin into microtubules. Taken together, these results suggest that NEK6 promotes directional cell growth through phosphorylation of β-tubulin and the resulting destabilization of cortical microtubules.
|
キーワード | Cell growth
Microtubules
Plant cytoskeleton
|
備考 | Published in Scientific Reports. 7, 7826(2017)
|
発行日 | 2017-08-10
|
出版物タイトル |
Scientific Reports
|
巻 | 7巻
|
出版者 | Nature Publishing Group
|
開始ページ | 7826
|
ISSN | 20452322
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
OAI-PMH Set |
岡山大学
|
著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
|
論文のバージョン | publisher
|
PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1038/s41598-017-08453-5
|