ID | 63450 |
フルテキストURL | |
著者 |
Satoh, Akira
Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Kaken ID
publons
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Kashimoto, Rena
Graduate School of Environmental and Life Science, Okayama University
Ohashi, Ayaka
Graduate School of Environmental and Life Science, Okayama University
Furukawa, Saya
Faculty of Science, Department of Biological Sciences, Okayama University
Yamamoto, Sakiya
Faculty of Science, Department of Biological Sciences, Okayama University
Inoue, Takeshi
Division of Adaptation Physiology, Faculty of Medicine, Tottori University
Hayashi, Toshinori
Amphibian Research Center, Hiroshima University
Agata, Kiyokazu
Laboratory of Regeneration Biology, National Institute for Basic Biology
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抄録 | Urodele amphibians, Pleurodeles waltl and Ambystoma mexicanum, have organ-level regeneration capability, such as limb regeneration. Multipotent cells are induced by an endogenous mechanism in amphibian limb regeneration. It is well known that dermal fibroblasts receive regenerative signals and turn into multipotent cells, called blastema cells. However, the induction mechanism of the blastema cells from matured dermal cells was unknown. We previously found that BMP2, FGF2, and FGF8 (B2FF) could play sufficient roles in blastema induction in urodele amphibians. Here, we show that B2FF treatment can induce dermis-derived cells that can participate in multiple cell lineage in limb regeneration. We first established a newt dermis-derived cell line and confirmed that B2FF treatment on the newt cells provided plasticity in cellular differentiation in limb regeneration. To clarify the factors that can provide the plasticity in differentiation, we performed the interspecies comparative analysis between newt cells and mouse cells and found the Pde4b gene was upregulated by B2FF treatment only in the newt cells. Blocking PDE4B signaling by a chemical PDE4 inhibitor suppressed dermis-to-cartilage transformation and the mosaic knockout animals showed consistent results. Our results are a valuable insight into how dermal fibroblasts acquire multipotency during the early phase of limb regeneration via an endogenous program in amphibian limb regeneration.
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キーワード | Pde4b
Limb regeneration
Pleurodels waltl
Ambystoma mexicanum
Dedifferentiation
Reprogramming
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発行日 | 2022-04-28
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出版物タイトル |
Zoological Letters
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巻 | 8巻
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号 | 1号
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出版者 | BMC
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開始ページ | 6
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ISSN | 2056-306X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2022.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1186/s40851-022-00190-6
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ライセンス | http://creativecommons.org/licenses/by/4.0/.
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | 20H03264
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