ID | 62437 |
フルテキストURL | |
著者 |
Nishida, Takashi
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Akashi, Sho
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takigawa, Masaharu
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
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抄録 | The renin-angiotensin system (RAS) controls not only systemic functions, such as blood pressure, but also local tissue-specific events. Previous studies have shown that angiotensin II receptor type 1 (AT(1)R) and type 2 (AT(2)R), two RAS components, are expressed in chondrocytes. However, the angiotensin II (ANG II) effects exerted through these receptors on chondrocyte metabolism are not fully understood. In this study, we investigated the effects of ANG II and AT(1)R blockade on chondrocyte proliferation and differentiation. Firstly, we observed that ANG II significantly suppressed cell proliferation and glycosaminoglycan content in rat chondrocytic RCS cells. Additionally, ANG II decreased CCN2, which is an anabolic factor for chondrocytes, via increased MMP9. In Agtr1a-deficient RCS cells generated by the CRISPR-Cas9 system, Ccn2 and Aggrecan (Acan) expression increased. Losartan, an AT(1)R antagonist, blocked the ANG II-induced decrease in CCN2 production and Acan expression in RCS cells. These findings suggest that AT(1)R blockade reduces ANG II-induced chondrocyte degeneration. Interestingly, AT(1)R-positive cells, which were localized on the surface of the articular cartilage of 7-month-old mice expanded throughout the articular cartilage with aging. These findings suggest that ANG II regulates age-related cartilage degeneration through the ANG II-AT(1)R axis.
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キーワード | angiotensin II
cellular communication network factor 2 (CCN2)
renin-angiotensin system (RAS)
losartan
angiotensin II type I receptor (AT(1)R)
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発行日 | 2021-08-25
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出版物タイトル |
International Journal of Molecular Sciences
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巻 | 22巻
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号 | 17号
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出版者 | MDPI
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開始ページ | 9204
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ISSN | 1422-0067
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2021 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.3390/ijms22179204
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Nishida, T.; Akashi, S.; Takigawa, M.; Kubota, S. Effect of Angiotensin II on Chondrocyte Degeneration and Protection via Differential Usage of Angiotensin II Receptors. Int. J. Mol. Sci. 2021, 22, 9204. https://doi.org/10.3390/ ijms22179204
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | 17K11641
20K09889
21H03105
19H03817
JP20K20611
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オープンアクセス(出版社) |
OA
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