Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

Kitamatsu, Mizuki; Kurami, Shunsuke; Ohtsuki, Takashi; Sisido, Masahiko

doi:10.1016/j.bmcl.2010.11.034

Permalink : http://escholarship.lib.okayama-u.ac.jp/46919

ID	46919
フルテキストURL	BMCL_21_1_225-227.pdf 131 KB
著者	Kitamatsu, Mizuki Department of Medical and Bioengineering, Graduate School of Natural Science and Technology Kaken ID publons researchmap Kurami, Shunsuke Department of Medical and Bioengineering, Graduate School of Natural Science and Technology Ohtsuki, Takashi Department of Medical and Bioengineering, Graduate School of Natural Science and Technology ORCID Kaken ID publons researchmap Sisido, Masahiko Department of Medical and Bioengineering, Graduate School of Natural Science and Technology
抄録	To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.
キーワード	Peptide nucleic acid Cell-penetrating peptide Antisense effect
発行日	2011-01
出版物タイトル	Bioorganic & Medicinal Chemistry Letters
巻	21巻
号	1号
出版者	Pergamon-Elsevier Science Ltd,
開始ページ	225
終了ページ	227
ISSN	0960-894X
NCID	AA1079577X
資料タイプ	学術雑誌論文
言語	英語
著作権者	© 2010 Elsevier Ltd. All rights reserved.
論文のバージョン	author
査読	有り
DOI	10.1016/j.bmcl.2010.11.034
PubMed ID	21112781
Web of Science KeyUT	000285544400043