ID | 62851 |
フルテキストURL | |
著者 |
Arimura, Yuki
Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Minato, Yusuke
Department of Microbiology, Fujita Health University School of Medicine
Wada, Takayuki
Department of Microbiology, Graduate School of Human Life Science, Osaka City University
Nakayama, Masaaki
Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ryumon, Ayako
Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Hirata, Nao
Department of Microbiology, Fujita Health University School of Medicine
Nakajima, Chie
Division of Bioresources, Hokkaido University International Institute for Zoonosis Control
Suzuki, Yasuhiko
Division of Bioresources, Hokkaido University International Institute for Zoonosis Control
Ato, Manabu
Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases
Kobayashi, Kazuo
Department of Immunology, National Institute of Infectious Diseases
Ohara, Naoko
Department of Operative Dentistry, Okayama University Hospital, Okayama University
Iida, Seiji
Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ohara, Naoya
Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.
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キーワード | thymidylate synthase
ThyX
Mycobacterium
BCG
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備考 | This is the peer reviewed version of the following article: [Arimura, Y, Minato, Y, Wada, T, Nakayama, M, Ryumon, A, Hirata, N, et al. Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG. Microbiol Immunol. 2021; 1– 5. https://doi.org/10.1111/1348-0421.12944], which has been published in final form at [https://doi.org/10.1111/1348-0421.12944]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages there of by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
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発行日 | 2021-10-21
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出版物タイトル |
Microbiology and Immunology
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巻 | 66巻
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号 | 1号
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出版者 | Wiley
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開始ページ | 10
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終了ページ | 14
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ISSN | 0385-5600
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NCID | AA00738350
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2021 The Societies and John Wiley & Sons Australia, Ltd
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1111/1348-0421.12944
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Citation | Arimura, Y, Minato, Y, Wada, T, Nakayama, M, Ryumon, A, Hirata, N, et al. Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG. Microbiol Immunol. 2021; 1– 5. https://doi.org/10.1111/1348-0421.12944
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