JaLCDOI 10.18926/40265
タイトル(別表記) Studies on Paper-Analysis in the Field of Balneology (I) Studies on Iron in Serum by Means of Parper-Electrophoresis and Paper-Chromatography
フルテキストURL 022_037_054.pdf
著者 石橋 丸応|
抄録 1. The author showed by means of paper-electrophoresis using o-nitrosoresorcinmonomethylether (hereafter N. R. M. E.), a colourdeveloping reagent, that iron in serum combines with β-fraction of serum protein. 2. The author proposed a new method for the determination of serum iron by means of paper-chromatography. Procedure is as follows. In a centriguged tube, 0.5 ml. of clear serum is taken, acidified with 0.15 ml. of 20% HNO(3), and allowed to stand for 20 minutes. After addition of 0.2 ml. of saturated solution of CH(3)COONa and 1 ml. of acetic acid-sodium acetate buffer solution (pH: 3.8), the mixture is kept for at least 10 min. and filtered through Toyo-filter-paper No.5 A. The clear filtrate is taken in a stoppered test tube (the inside diameter: 1.2 cm.; height: 15 cm.). To this filtrate are added 0.2 ml. of 20% Hydroxylamine hydrochloride and 0.25 ml. of saturated aqueous solution of N. R. M. E. After being allowed to stand for at least 20 min., the mixture is shaken with 5 ml. of carbon tebrachloride. Then the excess of N. R. M. E. is removed. The supernatant aqueous solution (0.2 ml.) is used for a determination sample. The sample is placed on the paper (Toyo,filter-paper No.51 or 50) in a thin line at a distance of 10 cm. from the edge, and the spots are developed with 90 % ethylalcohol. After development for 4-6 hours, a green-coloured linear spot appsars at a distance of 12-16 cm. from the origin-point. Spot intensity at 670 mμ is measured with Natsume's densitometer, and from this, concentration of Fe can be estimat.ed on the standard graph (Fig. 5). 3, Effects of radioactive thermal spring bathing and internal use of vitriol water upon serum iron levels were studied with the result.s as follows. The above-mentioned author's method of determining the iron level in a small amount of serum is very convenient in investigating iron metabolism in the field of balneotherapy. The author examined changes of iron levels in serum after radioactive thermal bathing as well as after internal use of acid vitriol water. a) The iron levels in serum were maasurecl by the author's method before and 5, 30 and 60 minutes after the radioactive thermal bath (Rn-content: 10-30 Mache, 42-3°C., for 10 min,). The iron levels in the serum of healthy subjects showed no significant change after the thermal bathing. b) Yanahara Mineral Water (an acid vitriol water, pH; 2.2) contains 0.045 gm. of Cl(-), 12.35 gm. of SO(4)(--), 2.5 gm. of Fe(++)+Fe(+++), 0.0938 gm. of Al(+++) and 0.0001 gm. of Cobalt in one liter. i) Thirty ml. of Yanahara, water diluted with plain water to 200 ml. (Fe(++) content: ca. 75 mg.) and administered to health fasting subjects orally. As a control matter, 0.65 gm. of Glukon-F powder (Fe(++) content.: ca. 75 mg.) was then given. Blood samples were taken from the cubital vein before and 1, 3 and 6 hours after the intake of the dtriol water. Iron contents of the serum samples were measured by the author's paper chromatographic method. The results were shown in the table and the figure. The iron contents of the serum after the intake of the mineral water were higher than in the case of the control. ii) Two ml. of Yanahara vitriol water was administered to rabbits with the aid of a stomach tube, and serum iron levels wera determined before and 1, 3, 4 and 6 hours after the administration. An amount of 0.05 gm. of Glukon-F powder was dissolved in 5 ml. of plain water, and this solution was given to rabbits as a control. After the internal use of vitriol water, iron levels in serum rose and remained unexpactedly high for about 1-4 hours, in a manner that did not. correspond to the amount of iron in the given mineral water. So that it was suspected that the iron originally contained in the living subjects had been mobilized by the intake of vitriol water. Iron levels in serum after administration of a solution of Glukon-F powder were lower than in the case of the mineral water.
出版物タイトル 岡山大学温泉研究所報告
発行日 1958-07
22巻
開始ページ 37
終了ページ 54
ISSN 0369-7142
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/40263
言語 Japanese
論文のバージョン publisher
NAID 40017532359
JaLCDOI 10.18926/40264
タイトル(別表記) Clinical and Experimental Studies of Therapeutic Effects of Radioactive Thermal Bath on Peripheric Disorders of Circulation Part 1 The Effect of Radioactive Thermal Bath upon Serum Cholesterol Levels
フルテキストURL 022_001_036.pdf
著者 松本 欣之|
抄録 The author investigated the effect of radioactive hot spring bath on serum cholesterol levels. The chemical compositions of the spring waters used are described in Table I. As experimental subjects healthy men and healthy white rabbits were used. The following results were obtained. 1) Single bath. The total and ester cholesterol levels in the serum were measured by a modification of Bloor's method before and 1/2, 1, 2 and 3 hours after the radioactive thermal bath (41-43°C., for 5 minutes). The concentration of total and ester cholesterol had decreased following radioactive thermal baths (Hisui-no-Yu and Kenkyusho-Sen), and remained at a decreased level for l/2 to 2 hours, but tended to return to normalcy within three hours from the time of the bath. (Fig. 2, 3, 4, 5.) The plain-water bath and vapour bath used as a control (41-43°C., for 5 minutes and 44-47°C. for 10-20 minutes respectively), had no significant effect on the blood cholesterol levels (Fig. 7 and 8). 2) Series of baths. The author examined weekly for 4 weeks the changes in serum cholesterol in healthy subjects following "a series of baths" in radioactive hot springs. The total and ester cholesterol levels of the serum decreased during the first and second weeks and tended to return to the initial levels during the third and fourth weeks (Fig. 11). The total cholesterol levels of the serum in rabbits following "a series of baths" in KenkyushoSen (Rn-content; 10-40 Mache) increased during the second week, but the cholesterol levels of the serum, after bathing in Hisui-no-Yu (Rn-content: 300-400 Mache), tended to decrease during the first and second weeks (Fig. 9 and 10). From this experiment, it is concluded that the content of radon in hot spring water affects the concentrations of the serum cholesterol levels. 3) Following an intramuscular injection of A. C. T. H. or Cortisone, the concentrations of total cholesterol and ester cholesterol had decreased and remained at the low level for 4 hours (Fig. 12. 13, 14 and 15). 4) It is said that the reticulo-endotherial system plays an important part in cholesterol metabolism. The author injectt 5 ml. of 1% Indian ink into the aureal vein of the rabbits for the purpose of blocking the reticulo-endotherial system. These procedures were carried out once a day for 7 successive days. The total cholesterol levels of the serum in the bathed group (bathing in Kenkyusho-sen once a day for 4 weeks, 43°C., 5 minntes) were lower than those of the control (Fig. 16). From these findings, the author thinks that the fall in the serum cholesterol levels following Misasa Hot spring bathing is due to the synthetic action of many factors such as the acceleration of oxydation in the living body, and the hyperfunction of liver cells, of hypophysealadrenocortical, of reticulo-endotherial systems and of various endocrine glands, caused by radioactive thermal bathing. Alternation of vegetative nervous system and various vitamine metabolism also take part in this phenomenon.
出版物タイトル 岡山大学温泉研究所報告
発行日 1958-07
22巻
開始ページ 1
終了ページ 36
ISSN 0369-7142
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/40263
言語 Japanese
論文のバージョン publisher
NAID 40017532360
著者 岡山大学温泉研究所|
発行日 1958-07
出版物タイトル 岡山大学温泉研究所報告
22巻
資料タイプ その他
著者 岡山大学温泉研究所|
発行日 1958-07
出版物タイトル 岡山大学温泉研究所報告
22巻
資料タイプ その他