ID | 32838 |
JaLCDOI | |
フルテキストURL | |
著者 |
Komori, Eisaku
Okayama University
Shoga, Kazuhiko
Okayama University
Aoe, Motoi
Okayama University
Sawada, Shigeki
Okayama University
Ichiba, Shingo
Okayama University
Shimizu, Nobuyoshi
Okayama University
|
抄録 | To elucidate the mechanism of anti-inflammatory effect of partial liquid ventilation (PLV), cytokine concentration, surface CD11b, and macrophage expression were investigated in BALF. The 30-minutes group was treated with gas ventilation (GV) for 30 minutes after intratracheal LPS administration. The GV group was prepared in the same manner as the 30-minutes group, then the GV was continued for the following 2 hours. The PLV group was treated in the same manner as the 30-minutes group, and then received PLV with perflubron for the following 2 hours. Animals were euthanized to receive BAL. The PLV group showed a tendency to have a higher concentration than the GV group of TNF-alpha, MIP-2, and CINC-1 as measured by ELISA, although there were no significant differences. The ratio of expressions of CD11b and macrophages to total leukocytes were determined by flow-cytometry. There were no significant differences in the ratio of CD11b-positive expression to acquired cells (GV: 63.6 +/- 8.4%, PLV: 60.5+/-5.4%, P=0.73). However, the proportion of macrophages was significantly increased (GV: 5.6 +/-1.5, PLV: 14.0+/-1.3, P=0.006). These results suggest that the anti-inflammatory effect of PLV is not caused by the change in CD11b expression, and that PLV affects the proportion of macrophage among BALF cells. |
キーワード | partial liquid ventilation
anti-inflammatory effect
BAl
cytokine
flow cytometry
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2003-06
|
巻 | 57巻
|
号 | 3号
|
出版者 | Okayama University Medical School
|
開始ページ | 133
|
終了ページ | 141
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |