ID | 31709 |
JaLCDOI | |
フルテキストURL | |
著者 |
Htut, Ye
Medical Research, Lower Myanmar, Yongon, Myanmar
Aye, Kyin Hla
Medical Research, Lower Myanmar, Yangon, Myanmar
Han, Kay Thwe
Medical Research, Lower Myanmar, Yangon, Myanmar
Kyaw, Myat Phone
Lower Myanmar
Shimono, Kunio
Shimono Hospital
Okada, Shigeru
Okayama University
|
抄録 | We studied parasite detectability in thick films by an acridine orange fluorescence technique (AO) to test its applicability and the use of a Malaria Diagnosis Microscope (MDM)-ESL in the detection of parasites, compared to the conventional Giemsa staining method. This study was conducted on 1,390 clinically suspected malaria cases of Thaton township, Myanmar. We found sensitivities of 82.8% for Plasmodium falciparum (P. falciparum) and 100% for Plasmodium vivax (P. vivax) and specificities of 97.1% for P. falciparum and 98.6% for P. vivax. AO had a higher sensitivity than Giemsa-stained films at low levels of parasitemia (< 1,000/microl). AO showed lower sensitivity and higher specificity than the Giemsa method at parasite levels of more than 1,000/microl. The results of using the AO method, achieved by both novice and experienced observers, showed no significant difference and required less practice to perform the test as well as to identify the parasite. The acridine orange fluorescence technique using a malaria diagnosis microscope MDM-ESL series is simple, rapid and cost effective. The microscope is conveniently operable using standard AC power or a 12-V DC car battery, and it is easily convertible to a conventional biological microscope. With the exception of species differentiation, which is not possible with this method, this method would be appropriate for both clinical and epidemiological studies. |
キーワード | malaria diagnosis
acridine orange
fluorescence
plasmodium
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2002-10
|
巻 | 56巻
|
号 | 5号
|
出版者 | Okayama University Medical School
|
開始ページ | 219
|
終了ページ | 222
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
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言語 |
英語
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論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |