ID | 31684 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ariyoshi, Masanori
Okayama University
MIzuno, Motowo
Okayama University
Morisue, Yoshiko
Okayama University
Shimada, Morizou
Okayama University
Fujita, Shirou
Okayama University
Nasu, Junichirou
Okayama University
Shimomura, Hiroyuki
Okayama University
Tsuji, Takao
Okayama University
|
抄録 | We developed a monoclonal antibody (MoAb) (clone 5E8) against an antigen on the bile canalicular membrane of rat hepatocyte. By immunoblotting, MoAb 5E8 detected a band of 110 kD. In this study, we used the phage display technique to identify the target antigen recognized by MoAb 5E8. We screened a random phage display library expressing 12-mer peptide sequences and identified a peptide sequence, FHFNPYTGHPLT, as an epitope. We compared this peptide sequence with those of dipeptidyl peptidase IV (DPP IV, E.C.3.4.14.5) and Cell-CAM105, which proteins were located by a database search based on the information of tissue localization and approximate molecular weight of the MoAb 5E8 antigen, and sequence similarity with a region in DPP IV (amino acids 225-233) but not with Cell-CAM105 was found. In addition, we immunohistochemically stained various tissues (liver, small intestine, and kidney) of Japanese Fischer 344 rats, known to be deficient for DPP IV, with MoAb 5E8 and showed that the expression of MoAb 5E8 antigen was negligible or weak. In contrast, tissues sampled from the same organs of Sprague-Dawley rats, known to express DPP IV, were positively stained. These findings suggest that the antigen recognized by MoAb 5E8 is DDPIV and its major epitope is located in amino acids at positions 225-233. |
キーワード | random phage display library
dipeptidyl petidase IV
monoclonal antibody
epitope
bile canalicular membrane
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2002-08
|
巻 | 56巻
|
号 | 4号
|
出版者 | Okayama University Medical School
|
開始ページ | 187
|
終了ページ | 191
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |