ID | 31682 |
JaLCDOI | |
フルテキストURL | |
著者 |
Shinji, Toshiyuki
Okayama University
Ujike, Kozo
Okayama University
Ochi, Koji
Okayama University
Kikui, Tetsuya
Okayama University
Matsumura, Naoki
Okayama University
Emori, Yasuyuki
Okayama University
Seno, Toshinobu
Okayama University
Koide, Norio
Okayama University
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抄録 | In studies of the pathogenesis of pancreatic fibrosis, pancreatic stellate cells (PSCs) have recently gained attention. In the present study, we established a new collagenase perfusion method through thoracic aorta cannulation to isolate PSCs, and we studied gene expression of TGF-beta1, type I collagen, and connective tissue growth factor using primary cultured PSCs. Our method facilitated PSC isolation, and by our new method, 4.3 +/- 1.2 x 10(6) PSCs were obtained from a rat. In comparing the expression of these genes with that of hepatic stellate cells (HSCs), we observed a similar pattern, although PSCs expressed type I collagen gene earlier than did HSCs. These results suggest that PSCs may play an important role in fibrosis of the pancreas, as HSCs do in liver fibrosis; in addition, PSCs may exist in a preactivated state or may be more easily activated than are HSCs. We also isolated the PSCs from a WBN/Kob rat, the spontaneous pancreatitis rat, and compared the gene expression with that from a normal rat.
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キーワード | pancreatic stellate cell
transforming growth factor beta
connective tissue growth factor
collagenase perfusion
WBN/Kob rat
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Amo Type | Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2002-08
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巻 | 56巻
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号 | 4号
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出版者 | Okayama University Medical School
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開始ページ | 211
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終了ページ | 218
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | Copyright© 2002 by Okayama University Medical School
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT |