ID | 31723 |
JaLCDOI | |
フルテキストURL | |
著者 |
Araki, Shinako
Okayama University
Miyagi, Yasunari
Okayama Red Cross General Hospital
Kawanishi, Kunihiro
Okayama University
Yamamoto, Junko
Okayama University
Yoshinouchi, Mitsuo
Okayama University
Kudo, Takafumi
Okayama University
|
抄録 | The in vitro radiosensitizing effects of docetaxel have been reported, but the DNA damage caused by the irradiation after docetaxel exposure has not been investigated. In this study, the authors attempted to evaluate the radiosensitizing effects in terms of cell survival and DNA single-strand breaks in a human ovarian adenocarcinoma cell line (known as line BG-1) and a human cervical squamous cell carcinoma cell line (known as line SiHa). The cell lines were exposed to various concentrations of docetaxel (from 2.27 x 10(-3) to 2.27 microg/ml) to investigate the cytocidal effects by colony-formation assay. DNA single-strand breaks after exposure to 2.27 microg/ml of docetaxel for 30 min or 100 min were measured by the alkaline-elution assay. The remarkable cytotoxicity of docetaxel followed by irradiation was observed when concentrations were greater than 2.27 x 10(-2) microg/ml in both cell lines. The combination of docetaxel and irradiation appears to be supraadditive. The DNA single-strand breaks induced by the irradiation were enhanced in both cell lines (BG-1; P < 0.01, SiHa; P < 0.05). The synergistic cytocidal effect cannot be explained quantitatively only by the single-strand breaks. |
キーワード | docetaxel
DNA single-strand break
radiosensitizer
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2002-02
|
巻 | 56巻
|
号 | 1号
|
出版者 | Okayama University Medical School
|
開始ページ | 13
|
終了ページ | 18
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |