ID | 31989 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ishii, Yasushi
Okayama University
Shimomura, Hiroyuki
Okayama University
Ito, Mamoru
Okayama University
Miyake, Masanobu
Okayama University
Ikeda, Fusao
Okayama University
Miyake, Jiro
Okayama University
Fujioka, Shin-ichi
Okayama University
Tsuji, Hideyuki
Okayama University
Tsuji, Takao
Okayama University
|
抄録 | It has been documented that the serum complement activities measured by hemolytic assay (CH50) are decreased after storage of sera at a low temperature in some patients with chronic hepatitis C. However, the mechanism of this phenomenon has not been identified yet. Here, we tried to elucidate factors involved in the cold activation of complement (CAC). To clarify what pathway is activated in CAC, we measured complement cleavage products after cold storage of sera. C4d increased significantly after 12 h-storage at cold temperatures in 5 CAC (+) sera compared with 5 CAC (-) (P < 0.01) and 3 control sera (P < 0.05), while Bb did not increase in any of the groups. In order to determine whether IgG or IgG complex is necessary for CAC, 8 CAC (+) sera were incubated with Protein G Sepharose gel beads, and all of them retained hemolytic activities to some extent after cold storage. Column chromatography through Superose 6HR of CAC-positive serum identified the fractions containing molecules that induced CAC in normal serum, which were depleted by treatment with protein G Sepharose. In conclusion, CAC in hepatitis C seems to occur via a classical or lectin pathway, and the IgG complex produced in hepatitis C virus infection may be an important factor in inducing CAC, a common extrahepatic manifestation of hepatitis C. |
キーワード | hepatitis C virus
chronic hepatitis
complement activation
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2001-08
|
巻 | 55巻
|
号 | 4号
|
出版者 | Okayama University Medical School
|
開始ページ | 229
|
終了ページ | 235
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |