ID | 32651 |
JaLCDOI | |
フルテキストURL | |
著者 |
Sato, Atsuhiko
Okayama University
Higashi, Toshihiro
Okayama University
Ling, Liu
Okayama Univerisity
Shiota, Tetsuya
Okayama University
Tsuji, Takao
Okayama University
|
抄録 | Indocyanine green (ICG) was injected into rat liver nodules induced by 2-acetylaminofluorene (2-AAF) via portal vein. The relationship between ICG staining and cell atypism of liver nodules was examined by means of histology and DNA flow cytometry. After 2-AAF administration, many small nodules appeared on the liver surface. All hyperplastic nodules were ICG stained until 10 weeks after the administration, but some nodules were not stained after 14 weeks. ICG-stained nodules histologically consisted of benign tissues and borderline lesions, and many of them showed "diploidy" in DNA cytometry. ICG-unstained nodules consisted of hepatocellular carcinoma (HCCs) and borderline lesions, and many of them showed "aneuploidy". In this way, it has been suggested that HCC could derive from hyperplastic nodules and that they might lose an ability to take up ICG in the process of hepatocarcinogenesis. Immunohistochemical staining for glutathione-S-transferase alpha (GST-alpha), a carrier protein of ICG in hepatocytes, was well correlated with ICG staining in the nodules, suggesting that the loss of ICG uptake in HCC was partly due to the decrease of GST-alpha. Moreover, the appearance of ICG unstained and aneuploid nodules was significantly inhibited in rats which were fed on diet containing Syosaiko-to after the administration of 2-AAF. Chemopreventive effect of Syo-saiko-to on hepatocarcinogenesis was identified. |
キーワード | hepatocellular carcinoma
idocyanine green
carcinogenesis
DNA flow cytometry
Syo-saiko-to
glutathione-S-transferase
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1992-04
|
巻 | 46巻
|
号 | 2号
|
出版者 | Okayama University Medical School
|
開始ページ | 57
|
終了ページ | 66
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |