ID | 30897 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ikeda, Takahiro
Okayama University
Ubaka, Toshihiko
Okayama University
Ishino, Kazushi
Okayama University
|
抄録 | The isoelectric point (pI) value of 3-mercaptopyruvate sulfurtransferase (MST) from human erythrocytes was determined to be 6.3 at 10 degrees C by isoelectric focusing in horizontal slab polyacrylamide gel containing 2% carrier ampholyte (pH 3-10). The value was determined by comparison with the electrofocused bands of bovine pancreatic ribonuclease A-glutathione mixed disulfides (RNase-SG), which were composed of 8 species containing 1 (RNase-SG1) through 8 (RNase-SG8) moles of glutathione per mole of ribonuclease A with different pI values ranging from 5.3 (RNase-SG8) to 8.8 (RNase-SG1). The pI value of the same enzyme in a 110,000 X g supernatant of rat liver was 5.9, which was the same as that of rat erythrocyte enzyme. Treatments of rat hemolysate with oxidized glutathione or diamide resulted in a shift of the pI of MST to a lower value, 5.7-5.5. This shift was inhibited when these treatments were performed in the presence of dithiothreitol. These results indicate that the treatment of the enzyme with oxidized glutathione results in the formation of enzyme-glutathione mixed disulfide. |
キーワード | 3-mercaptopyruvate sulfurtransferase
isoelectric point
glutathione
mixed disulfide
isoelectric focusing
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1989-02
|
巻 | 43巻
|
号 | 1号
|
出版者 | Okayama University Medical School
|
開始ページ | 1
|
終了ページ | 7
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID | |
Web of Science KeyUT |