ID | 32071 |
JaLCDOI | |
フルテキストURL | |
著者 |
Takahashi, Fumio
Okayama University
Kuramitsu, Makoto
Okayama University
Tokuda, Masaaki
Okayama University
Itano, Toshifumi
Okayama University
Murakami, Tetsu-Hide
Okayama University
Hatase, Osamu
Okayama University
Nishida, Isamu
Okayama University
|
抄録 | Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography. |
キーワード | chick growth factors
cell proliferation
growth regulation
DNA and RNA synthesis
protein synthesis
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1979-06
|
巻 | 33巻
|
号 | 3号
|
出版者 | Okayama University Medical School
|
開始ページ | 167
|
終了ページ | 176
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
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PubMed ID |