Acta Medica Okayama 19巻 2号

For the purpose to clarify whether or not the cells of regional lymph nodes and spleen of the tumor bearing individual develop the antitumor activity the author observed the proliferation of JTC-11 cells in vitro by mixing with the lymph-node and spleen cells from the mice bearing Ehrlich ascites tumor in solid form. After 24- to 48 -hour incubation the antitumor activity was estimated from the number of proliferated JTC-11 cells. As the result, it has been found that one week after implantation of tumor the regional lymph-node cells acquire the inhibitory activity against the proliferation of JTC-11 cells. The spleeen cells also show a marked inhibitory effect on the turner cell proliferation but two weeks after implantation these inhibitory activities of the cells both from lymph node and spleen are largely retarded three to four weeks when the host is emanciated by the growing tumors. Discussions are made on the inhibitory mechanisms from the viewpoint of immune reaction and on the transplantability of tumor cells without any rejection.

Almost all the umbilical lymphocytes showed more extensive blast cell formation than that of their mother's lymphocytes with PHA. Pathological conditions of mother in pregnancy and labor such as anemia, gestational toxicosis, difficult labor and asphyxia of babies, inhibited the normal response of both maternal and umbilical lymphocytes to PHA.

The mechanism of induction of anisocytosis was studied with experimental anemia of rabbits induced by blood depletion and phenylhydrazine chloride injection. The Price-Jones' curves of erythrocytes from anemic animals showed a large variety of red cell size, indicating the appearance of abnormally large sized cells. RNA contents of some reticulocytes in anemia were comparable to those of polychromatic and late basophilic erythroblasts. The result proved that in severe anemia a large number of erythroblasts are denucleated at earlier maturation stages, in most cases at polychromatic, and some at late basophilic and some at orthochromatic stages, resulting in anisocytosis.

Es wurde ein Triparanol-Präparat bei Arteriosklerose an 34 Patienten ambulant untersucht. Der Autor hat beim gröβten Teil seiner Falle eine wesentliche objektive und subjektive auch registrierdare Besserung beobaehtet. Der Blutcholesterin und der Blutdruek sind nicht nur bei den erfolgreiehen Fällen, sondern auch bei den wenigen Patienten, die Triparanol nicht vertragen konnten, gesunken, Oszillometrische, angiomyologisehe Werte und Hauftemperatur sind besser geworden. Schwinde1, Grübeln, Unsicherheit, stenokardische Beschwerden, schwermütige Gedanken, Benommenheit u. a. Symptome haden sich nur selten und nur in kleinem Maβe gezeigt.

Chemical and biological characteristics of the unsaturated fatty acids from the liver of irradiated and non-irradiated animals and some unsaturated fatty acids in sale have been described. The unsaturated fatty acid fractions from the rabbit liver taken after irradiating animal with x-ray show hardly any difference from those of non-irradiated animal in each component. But the former were distinguished from the latter in the increased rate of velocity of autoxidation. Similar characteristics were observed on the unsaturated fatty acids irradiated in vitro. They developed less labile free radicals with the shift of the double bonds to the carboxylic group and the conjugated double bonds, dienoic and trienoic acids. Biologically, the fatty acids from the irradiated animal suppressed the growth of bacteria requiring unsatturated fatty acid. And they are slightly stronger in the activity of uncoupling effect for the oxidative phosphorylation and the swelling of mitochondria comparing to those of general fatty acids, oleic and linoleic acids. They showed a strong lytic activity on the cell membrane as in the case of general fatty acids, linoleic, oleic, and some long chain unsaturated fatty acids. Tumor cells surviving through the treatment with unsaturated fatty acids changed the cell characteristics temporarily, with a slow-down of the ascites development and the cell growth.

In the experiments conducted with cells from the spleen and regional lymphoid cells derived from the axillary and regional lymph nodes of the mice receiving subcutaneous transplantation of Ehrlich tumor cells, it has been demonstrated that these lymphoid cells possess an ability to inhibit the proliferation of the subtrain of Ehrlich tumor cells (JTC-11), and that there is a parallel relationship between the rate of appearance of immunologicallly competent cells among these sensitized lymphoid cells and the inhibitory effect of these cells on JTC-11. In the experiment to reinforce the potency of sensitized cells conducted by means of the diffusion chamber technique, it has been proven that, while it is not an absolute prerogative, it is necessary to have the sensitized lymphoid cells come in contact with the target cells is order to wield the power of the former to the fullest extent. In the tissue cultures of lymphoid cells, it has been shown that sensitized lymphoid cells has a shorter life span than non-sensitized ones.