A study on the cytomorphologic structure of blood cells by vital staining I. Normal human blood cells in the bone marrow

Vital observation on the cellular morphology of the normal human blood cells was conducted by means of bone marrow culture successfully in conjunction with vital staining with Janus green B and neutral red. A special attention was paid for the alterations of the cellular structures in the course of the culture. The findings are summarized as follows : 1) Intracellular particles with affinity to Janus green B or neutral red were classified into minute granules, granules, vacuoles, and mitochondria. Morphologic features of each type of the particles were studied in detail. 2) Two types of granules are present in neutrophilic and eosinophilic blood cells, whereas one type of granules is present in basophilic blood cells. Eosinophilic and basophilic granules show characteristic pole formation in them at the terminal stage of the staining. 3) The rosette formation in the mature monocyte and the aggregations of neutral red vacuoles in the mature neutrophil and the mature lymphocyte were characterized. 4) The cluster of neutral red vacuoles is characteristic of the erythroblast. 5) The mitochondria of the mature neutrophil and the mature monocyte participate in producing neutral red vacuoles.