ID | 56145 |
フルテキストURL | |
著者 |
Sing’ombe Ombiro Geofrey
Graduate School of Environmental and Life Science, Okayama University
Sawai, Taku
Graduate School of Environmental and Life Science, Okayama University
Noutoshi, Yoshiteru
Graduate School of Environmental and Life Science, Okayama University
Nishina, Yuta
Research Core for Interdisciplinary Sciences, Okayama University
Matsui, Hidenori
Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro
Graduate School of Environmental and Life Science, Okayama University
Toyoda, Kazuhiro
Graduate School of Environmental and Life Science, Okayama University
Ichinose, Yuki
Graduate School of Environmental and Life Science, Okayama University
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抄録 | Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 μg/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 μg/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 μg/ml. Growth inhibition by these compounds was stable at pH 6–9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria.
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キーワード | Bactericide
Ralhibitins
Ralstonia solanacearum
Lead compounds
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備考 | This is an Accepted Manuscript of an article published by Elsevier
This fulltext will be available in June 2019
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発行日 | 2018-10
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出版物タイトル |
Microbiological Research
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巻 | 215巻
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出版者 | Elsevier
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開始ページ | 29
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終了ページ | 35
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ISSN | 0944-5013
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NCID | AA11017780
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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論文のバージョン | author
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DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1016/j.micres.2018.06.005
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