ID | 48823 |
フルテキストURL | |
著者 |
Koreishi, Mayuko
The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Honjo, Yasuko
The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Kaken ID
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Satoh, Ayano
The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
ORCID
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抄録 | Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods.
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キーワード | TEV protease
Golgi
golgins
microinjection
recombinant proteins
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発行日 | 2011-08-09
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出版物タイトル |
Antibody Technology Journal
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巻 | 2011巻
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号 | 1号
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出版者 | Dove Medical Press
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開始ページ | 5
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終了ページ | 11
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ISSN | 2230-3170
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://www.dovepress.com/detection-of-in-situ-cleaved-p115-with-the-cut-specific-antibodies-in--peer-reviewed-article-ANTI
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言語 |
英語
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著作権者 | © 2011 Koreishi et al
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
NAID |