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ID 8515
Eprint ID
8515
フルテキストURL
Thumnail K002988.pdf 49.3 KB
タイトル(別表記)
Des-gamma-carboxy Prothrombinは肝細胞癌のAutologousな増殖因子である
著者
鈴木 真由美 岡山大学
抄録
Des-gamma-carboxyl prothrombin (DCP) is a well recognized tumor marker for hepatocellular carcinoma (HCC). In the present study, we demonstrate that DCP has a mitogenic effect on HCC cell lines. Purified DCP stimulated DNA synthesis of Hep3B and SK-Hep-1 cells in a dose-dependent manner. DCP was found to bind with cell surface receptor Met causing Met autophosphorylation and also to activate STAT3 signaling pathway through Janus kinase 1. Luciferase gene reporter analysis showed that DCP induced STAT3-related transcription. Small interfering RNAs against both STAT3 and Met abrogated DCP-induced cell proliferation. DCP did not affect the mitogen-activated protein kinase pathway, Myc signaling pathway, or phosphoinositide 3-kinase/Akt pathway. Based on these results, we believe that DCP acts as an autologous mitogen for HCC cell lines. The Met-Janus kinase 1-STAT3 signaling pathway may be a major signaling pathway for DCP-induced cell proliferation.
備考
http://dx.doi.org/10.1074/jbc.M406714200
発行日
2005-06-30
出版物タイトル
資料タイプ
学位論文
学位授与番号
甲第2988号
学位授与年月日
2005-06-30
学位・専攻分野
博士(医学)
授与大学
岡山大学
オフィシャル URL
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15582995&dopt=Abstract
言語
Japanese
論文のバージョン
none
査読
不明