ID | 52269 |
フルテキストURL | |
著者 |
Shin-I, Tadasu
Natl Inst Genet
Seki, Motoaki
RIKEN, Plant Sci Ctr
Shinozaki, Kazuo
RIKEN, Plant Sci Ctr
Takeda, Kazuyoshi
Okayama Univ, Bioresources Res Inst
Yamazaki, Yukiko
Natl Inst Genet
Conte, Matthieu
Int Rice Res Inst, Crop Res Informat Lab
Kohara, Yuji
Natl Inst Genet
|
抄録 | A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E < 1E-5) to rice genes and Arabidopsis genes were 89 and 47%, respectively. Of the 5028 possible amino acid sequences derived from the 5006 FLcDNAs, 4032 (80.2%) were classified into 1678 GreenPhyl multigenic families. There were 555 cDNAs showing low homology to both rice and Arabidopsis. Gene ontology annotation by InterProScan indicated that many of these cDNAs (71%) have no known molecular functions and may be unique to barley. The cDNAs showed high homology to Barley 1 GeneChip oligo probes (81%) and the wheat gene index (84%). The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151-233 FLcDNAs on each of the seven barley chromosomes. These comprehensive barley FLcDNAs provide strong platform to connect preexisting genomic and genetic resources and accelerate gene identification and genome analysis in barley and related species.
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キーワード | full-length cDNA
Hordeum vulgare
mRNA
gene ontology
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発行日 | 2009-04
|
出版物タイトル |
DNA Research
|
巻 | 16巻
|
号 | 2号
|
出版者 | Oxford University Press
|
開始ページ | 81
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終了ページ | 89
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ISSN | 1340-2838
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | © The Author 2009. Kazusa DNA Research Institute.
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
Web of Science KeyUT |