ID | 48579 |
フルテキストURL | |
タイトル(別表記) | Study for the structures of the HA complexes produced by Clostridium botulinum type A mutant strain
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著者 |
馬 少博
岡山大学大学院医歯薬学総合研究科 病原細菌学
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抄録 | Clostridium botulinum produces seven neurotoxin (NTX) serotypes, classified from as A to G. In culture, NTX forms protein complexes by association with non-toxic components, such as nontoxic-nonhemagglutinin (NTNH) and hemagglutinins (HA1, HA2, HA3). C. botulinum serotype A produces three types of toxin complexes, M-toxin (NTX and NTNH), L-toxin (M-toxin and HAs) and LL-toxin (dimer of L-toxin). In this study, I found three HA complexes in the culture of a nontoxigenic mutant serotype A lacking ntx and ntnh expression. The HA complexes displayed similar banding patterns on SDS-PAGE, but the staining intensities of the HA1 and HA2 bands were different. In addition, their native-PAGE banding profiles exhibited different behaviors. The large-molecular-size HA complex showed the highest activity, similar to that of an L-toxin. Based on the combined results of the PAGE and gel-filtration profiles, the differences in molecular size among the three HA complexes were thought to be caused by different numbers of HA1 and HA2 molecules. This paper reports for the first time the purification and characterization of a native HA complex of serotype A, and suggests that the HA can form complex structures without NTX and NTNH. This may help in understanding the toxin complex assembly pathway.
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キーワード | ボツリヌス毒素(botulinum toxin)
ボツリヌス菌(Clostridium botulinum)
血球凝集素(hemagglutinin)
タンパク質複合体構造(protein complex structure)
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備考 | 原著(Original Paper)
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発行日 | 2012-08-01
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 124巻
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号 | 2号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 137
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終了ページ | 143
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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関連URL | http://www.okayama-u.ac.jp/user/oma/
http://ousar.lib.okayama-u.ac.jp/metadata/48452
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言語 |
日本語
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著作権者 | Copyright (c) 2012 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
Eprints Journal Name | joma
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