ID | 40925 |
フルテキストURL | |
タイトル(別表記) | Histochemical and Biochemical Studies on the Respiratory Enzymes Part 3. Histochemical Studies on Cytochrome Oxidase System in Various Tissues
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著者 |
岡崎 博明
岡山大学医学部病理学教室
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抄録 | Basing on the theory presented in the previous report, the author described the new methods for the histochemical and cytochemical demonstration of cytochrome c oxidase and of cytochrome c-cytochrome oxidase system using neotetrazolium chloride in the presence of p-phenylenediamine with or without additional cytochrome c, respectively. The reaction in fresh cell suspensions or in fresh tissue blocks appears as a distinct violet granules coinciding with the sites of mitochondria. The reaction in frozen tissue sections is not so distinct as that in cell suspensions and fresh tissue blocks, but histochemical observation on the distribution of the enzyme activity in the tissue can be made. By the combination of the histochemical method and the colorimetric method for the estimation of the enzyme activity, integrating studies on the morphologic structure and biochemical function in the respiratory activity in the living tissues are possible.
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発行日 | 1959-05-15
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 71巻
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号 | 6-2号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 3615
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終了ページ | 3621
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/71/6-2/71_6-2_3615/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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