高リポ蛋白血症(Ⅱ(a)型，Ⅱ(b)型，Ⅳ型)における脂質代謝についての研究―血液細胞による脂質合成について―

Six types of hyperlipoproteinemia were categorized by W.H.O. on the base of lipoprotein analysis by paper electrophoresis. In this study, the author presented the lipid metabolism by blood cells in each type of hyperlipoproteinemia (Ⅱa, Ⅱb, and Ⅳ type), comparing to control. As known generally, blood cells, mainly white cell, platelet and young red cell are able to synthesize lipids and fatty acids. And disorders in lipid metabolism were demonstrated in blood cells of some kinds of metabolic disease. Discussion were performed with analysis of the incorporation and distribution of l-(14)C-Acetate in major lipid (total lipid, cholesterol, triglyceride, cholesterol ester, phospholipid) and individual fatty acids by whole blood cells and white blood cell. Results were as follows. Ⅰ) Experiment on the metabolism by the whole blood cells. 1) The (14)C incorporation into total lipids were equally decreased by blood cell in every Ⅱa, Ⅱb and Ⅳ type compared to that of control, and particularly it was markedly decreased in Ⅱb type. 2) In contrast to the above, the (14)C incorporation into cholesterol ester and free cholesterol was increased in each Ⅱa, Ⅱb and Ⅳ type, compared to control. And furthermore, in Ⅳ type the highest incorporation was observed among them. 3) Concerning the distribution of (14)C in individual fatty acids, the de novo synthesized fatty acids were tend to decrease in Ⅱa, Ⅱb and Ⅳ type, and on the other hand the fatty acids synthesized by the elongation pathway were increased relatively. But no specific feature of (14)C distribution was obtained in fatty acids among them. Ⅱ) Experiment on the metabolism by white blood cells. 1) The (14)C recovered in total lipids was amounted one half of that of whole blood cells both calculated by 10(6) white cells. 2) There was significant differen in (14)C of both triglyceride and free fatty acid between white blood cell and whole blood cells. 3) White blood cell of Ⅱb and Ⅳ type synthesized more amount of TG than that of control did, and concomitantly was accompanied with the increase of cholesterol synthesis.