ID | 15939 |
Eprint ID | 15939
|
フルテキストURL | |
タイトル(別表記) | A study on the galactosyltransferase activity of human gastric mucosa Part 1. Assay conditions with ovomucoid as a substrate.
|
著者 | |
抄録 | The conditions assaying for UDP-galactosyltransferase in human gastric mucosa was studied. Ovomucoid without any pretreatment was employed as a substrate. The optimal pH was 6.8. Manganese, 2-mercaptoethanol and Triton X-100 were required for maximum enzyme activity. In the standard assay; 20 μl of enzyme solution was added to 30 μl of 50 mM MES buffer containing 5 mM 2-mercaptoethanol, 15 mM MnCl(2), 9.9mg protein/ml ovomucoid, 0.3 mM UDP-galactose, 1.7 μCi/ml UDP-[(3)H]-galactose and 1.5 mg/ml Triton X-100, and incubation was carried out at 37℃ for 20 min. [(3)H]-labelled ovomucoid was the only radioreactive reaction product detected. All galactose residue incorporated was liberated by β-galactosidase but not by α-galactosidase.
|
キーワード | human gastric mucosa
glycoprotein
UDP-galactosyltransferase
ovomuciod
N-acetylglucosamine
|
発行日 | 1987-06-30
|
出版物タイトル |
岡山医学会雑誌
|
出版物タイトル(別表記) | Journal of Okayama Medical Association
|
巻 | 99巻
|
号 | 5-6号
|
出版者 | 岡山医学会
|
出版者(別表記) | Okayama Medical Association
|
開始ページ | 657
|
終了ページ | 665
|
ISSN | 0030-1558
|
NCID | AN00032489
|
資料タイプ |
学術雑誌論文
|
オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/99/5-6/99_5-6_657/_article/-char/ja/
|
関連URL | http://www.okayama-u.ac.jp/user/oma/index.html
|
言語 |
日本語
|
著作権者 | 岡山医学会
|
論文のバージョン | publisher
|
査読 |
有り
|
Eprints Journal Name | joma
|