Enzyme-linked immunosorbent assay for detection of antibody to endotoxin in sera from patients with liver diseases

For the purpose of analyzing immune responses to endotoxin in liver diseases, an enzyme-linked immunosorbent assay (ELISA) employing microplates and hematocrit tubes for spectrophotometry was developed. The new ELISA allowed the analysis of extremely small serum samples (e.g. 10μl~100μl), and the assay had specificities for antibodies to various endotoxin antigens. The details of the method were presented. It was concluded that the new ELISA was useful clinically.