Journal of Okayama Medical Association
Published by Okayama Medical Association

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Full-text articles are available 3 years after publication.

Lysostaphin感受性によるStaphylococcus属の迅速鑑別について

Akatsuka, Kazuya
Ota, Kimi
Kayahara, Junko
Hiramoto, Hiroko
Fujimura, Hiroko
Kanemasa, Yasuhiro
Thumnail 97_19.pdf 835 KB
Abstract
The lysostaphin sensitivity test of staphylococci is a reliable classification method based on the differences in peptidoglycan compositions. Lysostaphin sensitive staphylococci are identified as S. aureus. In this paper, we stueied the applicability of this test to the rapid classification of clinically isolated staphylococci. Staphylococci were exposed to 2μg of lysostaphin per ml at 37℃ for 15 min. The sensitivity was decided by a turbidity check and gram-stain check. There were no differences between the check methods. Sixty-four strains of 99 clinical isolates were sensitive and consisted of 59 S. aureus. strains and 5 non-S. aureus strains (S. simulans 4; S. capitis 1). The 35 strains which were lysostaphin-insensitive were non-S. aureus as identified by coagulase and other biological sensitivity tests. The lysostaphin sensitivity test might be a very reliable and rapid method for the identification of S. aureus.
Keywords
Lysostaphin
Staphylococcus
Gram stain
Spectrophotometer
ISSN
0030-1558
NCID
AN00032489