Influence of phenobarbital and bucolome on biliary bilirubin

Biliary bilirubin conjugates were investigated in heterozygote Gunn rat after loading of phenobarbital or bucolome for 4 days. 50mg of phenobarbital or 200mg of bucolome per Kg of body weight was given intramusculary once a day. The bile collection by bile duct canulation was started 24 hours after the final drug loading and continued for 22 hours. The following results were obtained: 1) Bile volume increased significantly in both groups compared with the control group (no treatment group). The amount of excreted bilirubin increased significantly in the phenobarbital loading group than the control group, but not in the bucolome loading group. 2) A significantly increased amount of biliary bilirubin sulfate was observed in the Phenobarbital loading group compared with the control group, but not in the bucolome loading group. 3) The amount of excreted biliary bilirubin glucuronide increased significantly in the phenobarbital loading group compared with the control group, but not in the bucolome loading group.