Glycolytic enzymes: glucose-6-phosphate dehydrogenase, 6-phospho-gluconate dehydrogenase, lactate dehydrogenase and pyruvate kinase, of DMBA-induced mammary carcinoma, Shionogi Carcinoma 115 and Shionogi carcinoma 42. Glycolytic activities of these tissues were examined after hormonal ablation, administration of hormones and anti-hormonal drug. Enzymic activity of hormone dependent tumor was rised by administration of dependent hormones.