The Transformation of Energy Metabolism during the Maturation of Reticulocytes 2. The Relationship between Nucleotide Metabolism and Energy Metabolism during the Maturation of Reticulocytes

The present paper reported the relationship between nucleotide metabolism and energy metabolism during the maturation of rabbit reticulocytes and the following results were obtained: 1. The gradual degradation of adenine nucleotides during the maturation of reticulocytes was stimulated by the respiratory iuhibitors, antimycin A and amytal, or an uncouples of oxidative phosphorylation, 2, 4-dinitrophenol and an inhibitor, oligomyciin. 2. In these conditions the pentose moieties of adenine nucleotides were metabolized rapidly with the very large accumulation of hypoxanthine and lactic acid. 3. The processes of maturation of reticuloc t s, RNA decreases and the glycolysis were promoted by adding inosine and also by inosine monophosphate as well as by glucose. Theexogenous adenosine diphosphate and ribose-5-phosphate were hardly utilized for reticulocyte maturation. 4. The data suggest that pentose moieties of adenine nucleotides are reutilized effectively than glrcose during the reticulocyte maturation.