骨髄体外組織培養による白血病の螢光顕微鏡学的研究 第1編 骨髄螢光培養法による白血病の診断

50 cases of leukemic bone marrow were studied by the fluorochrominized tissue culture with acridine orange divised in our laboratory. Following results were obtained. 1. In acute leukemias, the growth area was extremely dense with migrating cells and its boundary was distinctly defined. It was characteristic in fluorochrominized tissue culture that the greater part of the growth area was green in color owing to the fluorescence of blast cells and its outer zone was somewhat reddish in acute myelogenous leukemia and yellowish in acute lymphocytic leukemia because of the presence of mature cells. 2. In chronic leukemias, the well-defined boundary of the growth area with a high cell density, which was also characteristic of acute leukemia, was sorrounded by another less dense zone of mature cells, forming a double growth zone. In chronic myelogenous leukemia, the inner zone of this doudle growth zone showed reddish green color, and in chronic lymphocytic leukemia yellowish green color. In the outer zone of this double growth zone the flnorescence of mature leukocytes was especially predominant. 3. In monocytic leukemia, the growth pattern was almost similar to that in aute lenkemia, but its fluorescent color was somewhat different. In the growth area there were many, greenish orange colored promonocytes and monocytes, green monoblasts, yellow lymphocytes and a few neutrophils with reddish orange granules. Therefore the growth area in monocytic leukemia disclosed multi-colored fluorescence. 4. To conclude these results, the growth area of each type of leukemia were occupied by the fluorescence of predominant leukemic cells. Accordingly by the fluorochrominized tissue culture of bone marrow the diagnosis of leukemia types could be performed.

学位番号 乙第172号