Studies on the Physiological Factors Participating on the Biosynthesis of Pyocianine by Pseudomonas aeruginosa

Pseudomonas aeruginosa produces the two pigments, namely Pyocianine and Fluorescin. The Pyocianine is bluish green pigment and is derivative of phenazine pigment. In this paper, the physiological factors that give influences on the biosynthesis of Pyocianine by Pseudomouas aeruginosa in culture will be presented. 1. Pseudomonas aeruginosa required glycerine as a nitrogene source for the biosynthesis of Pyocianine. In a case that glucose was used as carbon source, the addition of magnesium ion was essential. 2. Within alanine, glutamate and aspartate on which the author examined, only alanine was of effective as nitrogen source for the Pyocianine synthesis. 3. The following results have been investigated by the studies on the atmospheric condition. Pseudomonas aeruginosa produced the pigment at a aerobic condition in the chemically satisfactory culture media, but could not produce at anaerobic condition in any culture media. 4. In the acidic media below pH 6.0, the growing of bacteria was very slight and the Pyocianine could not be produced. Moreover, the pigment did not be synthesized when the pH was descended rapidly, even if the startiag pH of the medium was neutral or slight basic. 5. The growing of the bacteria is indispensable for the synthesis of Pyocianine, then the resting cells could not synthesize the pigment.