Studies on Tissue Culture of Human Embryonic Tissues and Their Susceptibility to Adenovirus Type 12 Part Ⅱ. Studies on the Susceptibility of Human Embryonic Tissue-Culture Cells to Adenovirus Type 12

Upon inoculation of adenovirus type 12 onto the primary culture cells of each of the kidney, the lung, the liver and the skin-muscle of a four-month-old human embryo, the following changes were observed: 1. In the cells of the kidney and the lung, rounding and aggregation of cells were observed. In those of the liver and the skin-muscle, rounding of cells was observed and, besides this, flat-round or -oval big cells appeared. 2. Such changes appeared earliest and strongest in the culture of kidney cells, next in that of lung cells and were very weak in those of liver and of skin-muscle cells. 3. Of these four human embryonic tissues, good multiplication of the virus was observed only in the culture of kidney cells.