Studies on Granulocytes in Bone-Marrow Tissue Culture by Fixation and Staining Part 1. On cover-slip method of bone-marrow tissue culture of normal rabbits and normal human sternum

In bone-marrow tissue culture of normal rabbits and of normal human sternum the author studied fixation and staining of the tissue in culture and also observed stain-findings of granulocytes and arrived at the following conclusions. 1. For the fixation and staining of the tissue in culture, the fixative of my own devise, namely, Zenker-glacial acetic acid-alcohol and Giemsa stain proved to give the least contraction of cells and to enable to differentiate bone-marrow granulocytes most readily. 2. The observations conducted from time to time by this method of fixation and staining give clearer pictures of the cell composition of cell growth area and bone-marrow granulocytes than vital observations. 3. Furthermore, by this method of observation it has been clarified that in bone-marrow tissue culture bone-marrow cells not only just wander out but also they mature and undergo cell division.