From the comparative biochmical standpoint the author isolated and estimated free amino acids and related substances in the brains of Hen (Gallus domesticus) and Common turtle (Geoclemys reevesii) in active stage, and obtaind the following results: 1. The free amino acid pattern of the hen brain is on the whole similar to that of the albino rat brain, containing X(3) and X(6) but no urea. 2. The free amino acid pattern of the common turtle brain reveals characteristics as the cold blooded animal and contains markedly greater volumes of aspartic acid, glutamic acid, glycine, alanine, glycerophosphoethanolamine, phosphoethanolamine and taurine as compared with those in the hibernating turtle. As for the volum of γ-amino butyric acid there can be recognized no difference between the brain of active turtle and that of hibernating one.