By the Bucker-Mirsky method the author estimated the pepsin-like substauce in urine, namely the content of uropepsin. with the purpose to elucidate the relationship between uropepsin and stress, the author estimated the uropepsin content in urine and studied the fluctuations in uropepsin; and the following are the results.
1. For the estimation of uropepsin the covering with toluol is not necessarily required for storing the urine to be tested, but it is preferable to keep the sample in a refrigirater and the estimation should be carried out as soon as possible. 2. As for the content of uropepsin in the urine drawn at a given interal, a fixed periodicity can be recognized in the fluctuations of the content, namely, diurnal changes. However, in the estimation of 24-hour urine the average amount excreted per hour resembles quite closely to the value of uropepsin in the urine drawn at 8 A. M. 3. Without any extreme change in foods or abrupt changes in environments the content of uropepsin excreted shows no diurnal changes. 4. In the case where the parson changes his residence from the accustomed place to a new strange place it has been found that the uropepsin value in urine rises temporarily, and after a subsequent period of transient fall the value returns to the normal level.