Histochemical and Biochemical Studies on the Respiratory Enzymes Part 2. Colorimetric Determinations of Cytochrome Oxidase Activity in Various Tissues

Basing on the theory presented in the previous report, the author described in the present report the new colorimetric methods for the estimation of the activities of cytochrome c oxidase and of cytochrome c-cytochrome oxidase system in tissue homogenates using neotetrazolium chloride in the presense of p-phenylenediamine with or without additional cytochrome c. Moreover, colorimetric estimation of cytochrome c-cytochrome oxidase system activity in various tissues such as the kidney, heart, liver, brains, and skeletal muscle was made using this methods.