Studies on the Isortion of Iron in the HbO(2)-KCN-H(2)O(2) Reaction System in Vitro Part II. Studies on So-Called Easy Split Iron (G. Barkan) in the Hb(2)-KCN-H(2)O(2) Reactionssystem

The vicissitude of easy split iron in the HbO(2)-KCN-H(2)O(2) reaction system put forward by G. Barkan was quantitatively observed, without the use of reducing agent. And the resulte were as follows. 1. The most proper condition grounded on the yielded dosis of easy split iron was KCN 1.0 M and H(2)O(2) 0.01 M. 2. Iron was separated from hemoglobin itself on H(2)O(2) over 0.1 M, in this reaction system. 3. The most proper condition was the pH 7.2 at 37°C, in this reaction system. 4. The change was not observed on the change of acting order between KCN and H(2)O(2), in this reaction system.