Studies on Anemia in Anchylostomiasis by means of Bone Marrow Tissue Culture Part 2. Bone Marrow Tissue Culture of Dogs with Anchylostomiasis

By means of bone marrow tissue culture, the author has investigated the hematopoietic activity and blood cell function in the bone marrow of the dogs experimentally infected with ca. 1000 hook worm larvae, which were classified into 3 groups of initial slight, moderate and severe anemia according to the level of anemia. 1) In a group of initial slight anemia, the growth rate was increased and the function of the neutrophils was accelerated in wandering velocity, phagocytosis of carbon particles and vital staining of neutral red. Wandering velocity of the eosinophils in the bone marrow was markedly accelerated. Increasing rate of the red blood cell count and hemoglobin content in fluid culture was slightly lowered. 2) In a group of moderate anemia with profuse intestinal bleeding, the growth rate was further increased, and wandering velocity was still kept in a high degree, but their function on phagocytosis of carbon particles and vital staining of neutral red was fairly lowered. Namely dissociation of the functions of neutrophils was observed in this group. Wandering velocity of the eosinophils was still elevated. Increasing rate of the red blood cell count was slightly elevated in comparison with that of the control one, but that of hemoglobin content being fairly lowered. 3) In a group of severe anemia with profuse intestinal bleeding and seriuos symptoms, the growth rate was most increased, wandering velocity being still kept nearly in the same level as that of the control one. But their function on phagocytosis of carbon particles and vital staining of neutral red was remarkably lowered. Wandering velocity of the eosinophils was reveresely lowered in proportion to the decrease of the eosinophils in peripheral blood. Increasing rate both of the red blood cell count and hemoglobin content was most lowered. 4) According to the above mentioned results, the author has confirmed that anemia in a group of initial slight anemia without intestinal bleeding was caused from blood cell arrest in the sinusoid of the bone marrow and disturbance of iron mobilization by hook worm toxin, but in groups of moderate and severe anemia with profuse intestinal bleeding, influences due to bleeding took a great role for the development of anemia besides the above mentioned mechanismus.