Studies on the Bile Composition and the Liver Tissue Part I Studies on the Liver Tissue effected with the Bile

The liver tissues of the healthy guinea pig and the guinea pig given a carbon tetrachloride or colloidal argent were effected with the bile of rabbit and we observed the prosperity and decay of the bile pigment in the bile and the cutting piececs of the liver tissues. The results are as follows: 1) The color of the bile gradually becomes changing a greenish yellow from a green through a yellowish green since one hour after the bile was effected with the liver tissues of healthy guinea pigs, but it is uchanged after 3 hours and the density of the bile is slightly decreased. At that time, the liver tissue is dyed and it's density becomes increased time by time, and the color is the same with the prosperity and decay of the bile, but the pigment is not reached over the 4 or 5 layers of the cell. The change of the color to yellow may be caused by the reduction to bilirubin from biliverdin according to the Gmelin reaction. The hematoidin cristal and granula in the edge of the liver cells around the liver aciuus, a part of the star cells and of the Glisson's capsules become appeared at the second hour, and they are decreased time by time after the third hour. Since the above observations, the transfer of the bile pigment into the liver tissue is not caused by a dye but it seems to be caused by a active absorption and it seems to become thickened. 2) The yellow color become strong more faster, and the appearance of hematoidin reaches into the central area of the acinus widely, as added sodium lactic acid into the bile prior to the expriments. 3) In the cases using of the liver tissues obstructed with a carbon tetrachloride, the dying condition of the tissues is the same with the normal cases, but the yellowish change of the bile and the dying pigment of the tissue is depressed and the reduction of biliverdin to bilirubin seems to be prolonged. And the hematoidin cristal and granula also do not appear in the regions described previously. 4) In the cases using of the liver tissues with the obstruction of the reticuloendothelial sysem, the dying condition of the liver tissue is the same with the normal cases, but the yellowish change of the bile and the dying pigment in the liver tissue is not observed, and the appearance of the hematoidin cristal and granula also is not observed.