Studies on the Protein Metabolism of the Explanted Bone Marrow Culture in Rabbits Part Three Influences of Sera from various Patients on the Protein Metabolism in the Rabbit Bone Marrow

By loading the tissue culture (Carrel flask method) of the bone marrow of normal rabbits with sera of Patients suffering from various diseases, various factors involved in multiplication of the bone marrow at the same time the influences of these of these sera on the protein metabolism in the rabbit bone marrow have been investigated and the following are the results: (1) Sera of hypolastic anemia, Banti's disease, and of leukemia act directly on the bone marrow, and the presence of the substances inhibiting the marrow functions has been recognized, inhibiting the production of γ-globulin and fibrinogen by the marrow. (2) No substance acting directly on the bone marrow has been recognized in the sera of such blood disorders as essential hypochromic anemia, hookworm disease, Werlhof's disease, as well as in liver and kidney diseases or tuberculosis, and the loading of such sera to the marrow culture exerts no influence on the protein metabolism. (3) In the serum of cancer patient, the presence of substance slightly inhibiting the marrow functions has been recognized.