The present work was carried on by means of 2 dimensional paper chromatography. ‘where phenol and lutidine-collidine were used. The following results were obtained. 1) Aspartic acid, glutamic acid, glutamine, γ-aminobntpric acid and taurine were remarkably demonstrated, glycine and alanine moderately, while serine, β-alanine, valine as well as leucine, slightly. 2) The free amino acids found in the brain are almost non-essential amino acids. 3) Certain fixed relation in quantitative ratio has been recognizable among glutamic acid, γ-aminobutyric acid and glutamine.