Study on the bile pigment originating from hemogrobin

I made some experiments on the bile pigment formed at the destruction of the hemogrobin. Method. 1-2% O-Hb Ringer's solution or 1-2% CO-Hb Ringer's solution were perfused through the liver of toads (Bufo japonicos), and then the bile. pigment in the bile duct was measured by the absorption band of the ultraviolet ray, by the absorption band of the visible ray and by the variation of colour. Summary. 1) When O-Hb Ringer's solution was perfused through the liver the bile pigment was elimineted in the bile duct. It is just as the normal bile pigment in colour, in the absorption band of the ultraviolet ray and in the absorption band of the visible ray. 2) The elimination of the bile pigment from the liver is influenced by the poisoning with cyanic acid, i.e. it ceases entirely. 3) If CO-Hb Ringer's solution is perfused through the liver bile pigment is not eliminated.

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