Bulletin of Faculty of Health Sciences Okayama University Medical School
Published by Faculty of Health Sciences Okayama University Medical School

<Formerly known as>
岡山大学医療技術短期大学部紀要 (1巻-9巻)

薬剤耐性細胞におけるアドリアマイシンの細胞内取り込みと細胞膜電位差の相関

Kawasaki, Shoji
Nishikawa, Koji
Shu Gao, Xian
Hiraki, Yoshio
Abstract
We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells.
Keywords
Adriamycin
Cell Membrane Potential
Flow Cytometry
ADR-Resistant Cells
DIDS
ISSN
0917-4494
NCID
AN10355371