start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=2 article-no= start-page=109 end-page=116 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship between Personality Traits and Postpartum Depressive Symptoms in Women who Became Pregnant via Infertility Treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract=The status of postpartum depression was elucidated herein with the use of the Edinburgh Postnatal Depression Scale (EPDS) in women in Shikoku, Japan who became pregnant and gave birth after undergoing infertility treatment, including assisted reproductive technology (ART). The assessment was performed during their children’s 4-month health examination. The relationships between postpartum depression and the mothers’ background factors and scores on the Big Five personality traits scale were also examined. Of the Big Five personality traits, the scores for neuroticism were significantly higher in the ART group (n=71) than in the general infertility treatment (n=118) and natural pregnancy (n=872) groups. No significant differences in EPDS scores were seen among these three groups. A logistic regression analysis showed that neuroticism was associated with an EPDS score ≧9 points, (which is suggestive of postpartum depression, ) in all groups. Moreover, although a long-standing marriage had an inhibitory effect on postpartum depression in the natural pregnancy group, no such trend was seen in the ART group, which included many women with long-standing marriages. Particularly for women who become pregnant by ART, an individualized response that pays close attention to the woman’s personality traits is needed. en-copyright= kn-copyright= en-aut-name=AwaiKyoko en-aut-sei=Awai en-aut-mei=Kyoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakatsukaMikiya en-aut-sei=Nakatsuka en-aut-mei=Mikiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Health Sciences, Okayama University kn-affil= affil-num=2 en-affil=Faculty of Health Sciences, Okayama University kn-affil= en-keyword=infertility treatment kn-keyword=infertility treatment en-keyword=assisted reproductive technology kn-keyword=assisted reproductive technology en-keyword=postpartum kn-keyword=postpartum en-keyword=postpartum depression kn-keyword=postpartum depression en-keyword=personality trait kn-keyword=personality trait END start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=2 article-no= start-page=93 end-page=100 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lower Work Engagement Is Associated with Insomnia, Psychological Distress, and Neck Pain among Junior and Senior High School Teachers in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=School teachers are subject to both physical and mental health problems. We examined cross-sectional relationships between work engagement and major health outcomes among junior and senior high school teachers in Japan via a nationwide survey in 2019-2020. A total of 3,160 respondents were included in the analyses (19.9% response rate). Work engagement was assessed with the Utrecht Work Engagement Scale-9 (UWES-9), and we thus divided the teachers into quartiles according to their UWES-9 scores. Based on validated questionnaires, we assessed insomnia, psychological distress, and neck pain as health outcomes. A binomial logistic regression adjusted for age, gender, school type, teacher’s roles, involvement in club activities, division of duties, employment status, and whether they lived with family demonstrated that the teachers with lower UWES-9 scores had higher burdens of insomnia, psychological distress, and neck pain (odds ratios [95% confidence intervals] in 4th vs. 1st quartile, 2.92 (2.34-3.65), 3.70 (2.81-4.88), and 2.12 (1.68-2.68), respectively; all trend p<0.001). There were no significant differences in these associations between full-time and part-time teachers. Our findings indicate that low work engagement may contribute to physical and mental health issues among junior and senior high school teachers, thus providing insights for preventing health problems in this profession. en-copyright= kn-copyright= en-aut-name=TsuchieRina en-aut-sei=Tsuchie en-aut-mei=Rina kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FukudaMari en-aut-sei=Fukuda en-aut-mei=Mari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsumuraHideki en-aut-sei=Tsumura en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KinutaMinako en-aut-sei=Kinuta en-aut-mei=Minako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HisamatsuTakashi en-aut-sei=Hisamatsu en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KandaHideyuki en-aut-sei=Kanda en-aut-mei=Hideyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Psychology, Graduate School of Technology, Industrial and Social Sciences, Tokushima University kn-affil= affil-num=4 en-affil=Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=work engagement kn-keyword=work engagement en-keyword=school teachers kn-keyword=school teachers en-keyword=insomnia kn-keyword=insomnia en-keyword=psychological distress kn-keyword=psychological distress en-keyword=neck pain kn-keyword=neck pain END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Novel pulmonary abdominal normothermic regional perfusion circuit for simultaneous in-donor evaluation and preservation of lungs and abdominal organs in donation after circulatory death en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objective To overcome limitations of traditional ex vivo lung perfusion (EVLP) for controlled donation after circulatory death (cDCD) lungs, this study aimed to evaluate a novel pulmonary abdominal normothermic regional perfusion (PANRP) technique, which we uniquely designed, for in situ assessment of lungs from cDCD donors.
Methods We modified the abdominal normothermic regional perfusion circuit for simultaneous lung and abdominal organ assessment using independent extracorporeal membrane oxygenation components. Blood was oxygenated via a membrane oxygenator and returned to the body, with pulmonary flow adjusted to maintain pressure < 25 mmHg. Femoral cannulation was performed, and the lungs were ventilated with standard settings. Organ function was assessed over 2 h using PaO2/FiO2, AST, ALT, BUN, and Cr measurements to monitor perfusion and oxygen delivery.
Results PANRP maintained stable lung function, with P/F ratios above 300, and preserved abdominal organ parameters, including stable AST, ALT, BUN, and Cr levels. Adequate urine output was observed, indicating normal renal function. Pulmonary artery pressure remained < 20 mmHg, and pulmonary vascular resistance was kept at 400 dyn・s/cm5, showing no signs of lung dysfunction or injury throughout the circuit.
Conclusions PANRP offers a promising alternative to traditional EVLP for cDCD lung evaluation, allowing in situ assessment of multiple organs simultaneously. This approach may overcome logistical and economic challenges associated with ex vivo techniques, enabling a more efficient evaluation process. Further studies are warranted to confirm its clinical applicability and impact on long-term outcomes. en-copyright= kn-copyright= en-aut-name=TanakaShin en-aut-sei=Tanaka en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UmedaMasashi en-aut-sei=Umeda en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UjikeHiroyuki en-aut-sei=Ujike en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=RyukoTsuyoshi en-aut-sei=Ryuko en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TomiokaYasuaki en-aut-sei=Tomioka en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyoshiKentaroh en-aut-sei=Miyoshi en-aut-mei=Kentaroh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkazakiMikio en-aut-sei=Okazaki en-aut-mei=Mikio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SugimotoSeiichiro en-aut-sei=Sugimoto en-aut-mei=Seiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ToyookaShinichi en-aut-sei=Toyooka en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=2 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=3 en-affil=Department of General Thoracic Surgery, Shimane University Graduate School of Medicine kn-affil= affil-num=4 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=5 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=6 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=7 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=8 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= affil-num=9 en-affil=Department of General Thoracic and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= en-keyword=Lung preservation kn-keyword=Lung preservation en-keyword=Donation after circulatory death kn-keyword=Donation after circulatory death en-keyword=Abdominal normothermic regional perfusion kn-keyword=Abdominal normothermic regional perfusion END start-ver=1.4 cd-journal=joma no-vol=79 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Endothelial Cell Polarity in Health and Disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=Endothelial cell polarity is fundamental to the organization and function of blood vessels, influencing processes such as angiogenesis, vascular stability, and response to shear stress. This review elaborates on the molecular mechanisms that regulate endothelial cell polarity, focusing on key players like the PAR polarity complex and Rho family GTPases. These pathways coordinate the front–rear, apical–basal and planar polarity of endothelial cells, which are essential for the proper formation and maintenance of vascular structures. In health, endothelial polarity ensures not only the orderly development of blood vessels, with tip cells adopting distinct polarities during angiogenesis, but also ensures proper vascular integrity and function. In disease states, however, disruptions in polarity contribute to pathologies such as coronary artery disease, where altered planar polarity exacerbates atherosclerosis, and cancer, where disrupted polarity in tumor vasculature leads to abnormal vessel growth and function. Understanding cell polarity and its disruption is fundamental not only to comprehending how cells interact with their microenvironment and organize themselves into complex, organ-specific tissues but also to developing novel, targeted, and therapeutic strategies for a range of diseases, from cardiovascular disorders to malignancies, ultimately improving patient outcomes. en-copyright= kn-copyright= en-aut-name=ThihaMoe en-aut-sei=Thiha en-aut-mei=Moe kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HikitaTakao en-aut-sei=Hikita en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakayamaMasanori en-aut-sei=Nakayama en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Pathophysiology and Drug Discovery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pathophysiology and Drug Discovery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Pathophysiology and Drug Discovery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=blood vessel kn-keyword=blood vessel en-keyword=endothelial cell kn-keyword=endothelial cell en-keyword=cell polarity kn-keyword=cell polarity en-keyword=atherosclerosis kn-keyword=atherosclerosis en-keyword=cancer kn-keyword=cancer END start-ver=1.4 cd-journal=joma no-vol=18 cd-vols= no-issue=1 article-no= start-page=19 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Assessment of the renal function of patients with anorexia nervosa en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background A decreased glomerular filtration rate (GFR), estimated using creatinine (Cr- eGFR), is often found at the initial presentation of anorexia nervosa (AN). Its pathophysiology has been explained mainly by dehydration, and chronic hypokalemia is also thought to be a cause. However, because we have often experienced cases of AN with decreased Cr-eGFR without these conditions, we must consider different etiologies. The focus of this paper is on low free triiodothyronine (FT3) syndrome. We also discuss the utility of eGFR, estimated using cystatin-C (CysC-eGFR), for these patients.
Methods The data of 39 patients diagnosed with AN between January 2005 and December 2023 was available for study. The characteristics of patients at the lowest and highest body mass index standard deviation score (BMI-SDS) were examined. Data on the parameters Cr-eGFR, CysC-eGFR, dehydration markers, potassium (K), and hormonal data and BMI-SDS were assessed during the treatment course to evaluate the correlations in these parameters. Blood hematocrit, uric acid (UA), blood urine nitrogen (BUN) level, and urine specific gravity were adopted as dehydration markers; FT3, free thyroxine, thyroid stimulating hormone, and insulin-like growth factor were adopted as hormonal data. Cr-eGFR and simultaneously evaluated dehydration markers, K, or hormonal data were extracted and correlations associated with the changes in BMI-SDS were examined. Furthermore, Cr-eGFR and simultaneously assessed CysC-eGFR were compared.
Results When the BMI-SDS was at the lowest value, low-FT3 syndrome was shown. Severe hypokalemia was not found in our study. A linear relation was not found between Cr-eGFR and BMI-SDS. A statistically significant correlation was found between Cr-eGFR and FT3 (p = 0.0025). Among the dehydration markers, statistically significant correlations were found between Cr-eGFR and BUN or UA. The difference between Cr-eGFR and CysC-eGFR was prominent, and CysC-eGFR showed much higher values.
Conclusions Our data indicates that low-FT3 syndrome and dehydration were related to the renal function of our patients with AN. Furthermore, our data suggest that caution is needed in the interpretation of kidney function evaluation when using CysC-eGFR in cases of AN. en-copyright= kn-copyright= en-aut-name=MiyaharaHiroyuki en-aut-sei=Miyahara en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShigeyasuYoshie en-aut-sei=Shigeyasu en-aut-mei=Yoshie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiiChikako en-aut-sei=Fujii en-aut-mei=Chikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaChie en-aut-sei=Tanaka en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ManaHanzawa en-aut-sei=Mana en-aut-mei=Hanzawa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SugiharaAkiko en-aut-sei=Sugihara en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkadaAyumi en-aut-sei=Okada en-aut-mei=Ayumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TsukaharaHirokazu en-aut-sei=Tsukahara en-aut-mei=Hirokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Clinical Pediatrics, Okayama University Academic Field of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=6 en-affil=Clinical Psychology Section, Department of Medical Support, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Pediatrics, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine kn-affil= en-keyword=Anorexia nervosa kn-keyword=Anorexia nervosa en-keyword=Dehydration kn-keyword=Dehydration en-keyword=Glomerular filtration rate estimated using creatinine kn-keyword=Glomerular filtration rate estimated using creatinine en-keyword=Glomerular filtration rate estimated using cystatin-C kn-keyword=Glomerular filtration rate estimated using cystatin-C en-keyword=Hypokalemia kn-keyword=Hypokalemia en-keyword=Low free triiodothyronine syndrome kn-keyword=Low free triiodothyronine syndrome END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=4 article-no= start-page=349 end-page=355 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=202408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Middle-Ear Salivary Gland Choristoma with Congenital, Single-Sided Hearing Loss en-subtitle= kn-subtitle= en-abstract= kn-abstract=Middle-ear salivary gland choristoma (SGCh) is a rare, benign tumor that causes conductive hearing loss owing to middle-ear morphological abnormalities. Early diagnosis is challenging, and surgical resection is indispensable for a definitive diagnosis. We report the case of a 3-year-old boy diagnosed with middle-ear SGCh during the follow-up period for left-sided hearing loss discovered at newborn hearing screening (NHS). Long-term follow-up after the NHS result, subsequent computed tomography/magnetic resonance imaging, and surgical resection led to its relatively early diagnosis and treatment. en-copyright= kn-copyright= en-aut-name=TominagaYuichiro en-aut-sei=Tominaga en-aut-mei=Yuichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SugayaAkiko en-aut-sei=Sugaya en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KariyaShin en-aut-sei=Kariya en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimizuAiko en-aut-sei=Shimizu en-aut-mei=Aiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KataokaYuko en-aut-sei=Kataoka en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AndoMizuo en-aut-sei=Ando en-aut-mei=Mizuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Otolaryngology, Head and Neck Surgery, Hiroshima City, Hiroshima Citizens Hospital kn-affil= affil-num=2 en-affil=Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Otolaryngology, Head and Neck Surgery, Kawasaki Medical School Hospital kn-affil= affil-num=4 en-affil=Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Otolaryngology, Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=middle-ear salivary gland choristoma kn-keyword=middle-ear salivary gland choristoma en-keyword=middle-ear morphological abnormalities kn-keyword=middle-ear morphological abnormalities en-keyword=newborn hearing screening kn-keyword=newborn hearing screening en-keyword=unilateral hearing loss kn-keyword=unilateral hearing loss en-keyword=surgical resection kn-keyword=surgical resection END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=9 article-no= start-page=1261 end-page=1267 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240501 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Severe Cytokine Release Syndrome and Immune Effector Cell-associated Neurotoxicity Syndrome in a Man Receiving Immune Checkpoint Inhibitors for Lung Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 55-year-old man with stage IV lung adenocarcinoma was treated with cisplatin, pemetrexed, nivolumab, and ipilimumab. Approximately 100 days after treatment initiation, he became disoriented and presented to the emergency department with a high fever. Blood tests revealed liver and kidney dysfunctions. Subsequently, the patient developed generalized convulsions that required intensive care. He was clinically diagnosed with cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Organ damage was gradually controlled with immunosuppressive drugs, including steroids, and the patient was discharged. Successful treatment is rare in patients with CRS, including ICANS, during immune checkpoint inhibitor treatment for solid tumors. en-copyright= kn-copyright= en-aut-name=TanakaTakaaki en-aut-sei=Tanaka en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TaokaMasataka en-aut-sei=Taoka en-aut-mei=Masataka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MakimotoGo en-aut-sei=Makimoto en-aut-mei=Go kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NinomiyaKiichiro en-aut-sei=Ninomiya en-aut-mei=Kiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HigoHisao en-aut-sei=Higo en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=FujiiMasanori en-aut-sei=Fujii en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IchiharaEiki en-aut-sei=Ichihara en-aut-mei=Eiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhashiKadoaki en-aut-sei=Ohashi en-aut-mei=Kadoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HottaKatsuyuki en-aut-sei=Hotta en-aut-mei=Katsuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TabataMasahiro en-aut-sei=Tabata en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=MaedaYoshinobu en-aut-sei=Maeda en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=4 en-affil=Center for Comprehensive Genomic Medicine, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=9 en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital kn-affil= affil-num=10 en-affil=Center for Clinical Oncology, Okayama University Hospital kn-affil= affil-num=11 en-affil=Department of Hematology, Oncology, and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=immune checkpoint inhibitor kn-keyword=immune checkpoint inhibitor en-keyword=nivolumab kn-keyword=nivolumab en-keyword=ipilimumab kn-keyword=ipilimumab en-keyword=cytokine release syndrome kn-keyword=cytokine release syndrome en-keyword=immune effector cell-associated neurotoxicity syndrome kn-keyword=immune effector cell-associated neurotoxicity syndrome END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=28201 end-page=28211 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=WLAN Channel Status Duration Prediction for Audio and Video Services Using Probabilistic Neural Networks en-subtitle= kn-subtitle= en-abstract= kn-abstract=Due to massive increase in wireless access from smartphones, IoT devices, WLAN is aiming to improve its spectrum efficiency (SE) using many technologies. Some interesting techniques for WLAN systems are flexible allocation of frequency resource and cognitive radio (CR) techniques which expect to find more useful spectrum resource by modeling and then predicting of channel status using the captured statistics information of the used spectrum. This paper investigates the prediction accuracy of busy/idle duration of two major wireless services: audio service and video service using neural network based predictor. We first study the statistics distribution of their time-series busy/idle (B/I) duration, and then analyze the predictability of the busy/idle duration based on the predictability theory. Then, we propose a data categorization (DC) method which categorizes the duration of recent B/I duration according the their ranges to make the duration of next data be distributed into several streams. From the predictability analysis of each stream and the prediction performance using the probabilistic neural network (PNN), it can be confirmed that the proposed DC can improve the prediction accuracy of time-series data in partial streams. en-copyright= kn-copyright= en-aut-name=HouYafei en-aut-sei=Hou en-aut-mei=Yafei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DennoSatoshi en-aut-sei=Denno en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Faculty of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= en-keyword=Wireless LAN kn-keyword=Wireless LAN en-keyword=Wireless communication kn-keyword=Wireless communication en-keyword=Media streaming kn-keyword=Media streaming en-keyword=Wireless sensor networks kn-keyword=Wireless sensor networks en-keyword=Resource management kn-keyword=Resource management en-keyword=Probability distribution kn-keyword=Probability distribution en-keyword=Channel allocation kn-keyword=Channel allocation en-keyword=Audio-visual systems kn-keyword=Audio-visual systems en-keyword=Data processing kn-keyword=Data processing en-keyword=Predictive models kn-keyword=Predictive models en-keyword=Neural networks kn-keyword=Neural networks en-keyword=Channel status duration prediction kn-keyword=Channel status duration prediction en-keyword=WLAN audio/video traffic kn-keyword=WLAN audio/video traffic en-keyword=data predictability analysis kn-keyword=data predictability analysis en-keyword=probabilistic neural network (PNN) kn-keyword=probabilistic neural network (PNN) END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=1 article-no= start-page=47 end-page=52 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=202402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Long-Term Follow-up Data of a Multi-Institutional Phase-2 Study of S-1/oxaliplatin and Bevacizumab Therapy in Patients with Advanced Colorectal Cancer: The HiSCO-02 Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Oral fluoropyrimidines (FUs) have certain advantages over intravenous FUs, such as longer intervals between outpatient visits, no requirement for central venous port (CVP) implantation, and lower incidence of neutropenia. We previously reported the efficacy of S-1/oxaliplatin (SOX) with bevacizumab therapy as a first-line treatment for advanced colorectal cancer (CRC) in a prospective phase-II multi-institutional clinical trial (HiSCO-02 study). However, our prognostic data at the time lacked a sufficient observation period. Herein, we analyze the longer-term follow-up data, focusing on the status of eventual CVP implantation via an open-label, non-randomized, multicenter study. This study enrolled 55 patients (mean age, 64 years), of whom 43 died (41 of primary cancer). The median overall survival was 22.7 months (95% CI: 20.1-34.7 months). Post-treatment regimens after failure of first-line treatment were initiated in 43 patients; CPT11-based regimens were selected in most cases, and other oral FU combinations in nine. CVP was implanted in 35 patients prior to first-line treatment; eleven of the remaining 20 patients did not require CVP implantation. In conclusion, we report here the final prognostic update of the Phase II clinical trial examining the efficacy of SOX plus bevacizumab therapy, the results of which confirm the clinical efficacy of this regimen. en-copyright= kn-copyright= en-aut-name=ShimomuraManabu en-aut-sei=Shimomura en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShinozakiKatsunori en-aut-sei=Shinozaki en-aut-mei=Katsunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YanoTakuya en-aut-sei=Yano en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkabaneShintaro en-aut-sei=Akabane en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhdanHideki en-aut-sei=Ohdan en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Hiroshima Surgical study group of Clinical Oncology (HiSCO) en-aut-sei=Hiroshima Surgical study group of Clinical Oncology (HiSCO) en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University kn-affil= affil-num=2 en-affil=Division of Clinical Oncology, Hiroshima Prefectural Hospital kn-affil= affil-num=3 en-affil=Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University kn-affil= affil-num=4 en-affil=Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University kn-affil= affil-num=5 en-affil=Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University kn-affil= affil-num=6 en-affil= kn-affil= en-keyword=metastatic colorectal cancer kn-keyword=metastatic colorectal cancer en-keyword=chemotherapy kn-keyword=chemotherapy en-keyword=S-1 kn-keyword=S-1 en-keyword=prospective phase II study kn-keyword=prospective phase II study END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=1 article-no= start-page=1 end-page=8 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=202402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Role of Macrophages in Liver Fibrosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Liver fibrosis, which ultimately leads to liver cirrhosis and hepatocellular carcinoma, is a major health burden worldwide. The progression of liver fibrosis is the result of the wound-healing response of liver to repeated injury. Hepatic macrophages are cells with high heterogeneity and plasticity and include tissue-resident macrophages termed Kupffer cells, and recruited macrophages derived from circulating monocytes, spleen and peritoneal cavity. Studies have shown that hepatic macrophages play roles in the initiation and progression of liver fibrosis by releasing inflammatory cytokines/chemokines and pro-fibrogenic factors. Furthermore, the development of liver fibrosis has been shown to be reversible. Hepatic macrophages have been shown to alternately regulate both the regression and turnover of liver fibrosis by changing their phenotypes during the dynamic progression of liver fibrosis. In this review, we summarize the role of hepatic macrophages in the progression and regression of liver fibrosis. en-copyright= kn-copyright= en-aut-name=SunCuiming en-aut-sei=Sun en-aut-mei=Cuiming kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsukawaAkihiro en-aut-sei=Matsukawa en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Pathology and Experimental Medicine, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pathology and Experimental Medicine, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=ERK-MAPK kn-keyword=ERK-MAPK en-keyword=SPRED2 kn-keyword=SPRED2 en-keyword=fibrosis kn-keyword=fibrosis en-keyword=macrophages kn-keyword=macrophages END start-ver=1.4 cd-journal=joma no-vol=1278 cd-vols= no-issue= article-no= start-page=341723 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20231016 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Determination of mass-dependent chromium isotopic compositions in geological samples by double spike-total evaporation-thermal ionization mass spectrometry (DS-TE-TIMS) en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background: Chromium isotopes have been used to trace geochemical and cosmochemical processes in the past. However, the presence of multivalent Cr species has made it difficult to isolate Cr from geological samples, particularly for samples with a low Cr mass fraction.
Results: Here, a simple three-step ion exchange chromatography procedure is presented to separate Cr from various sample matrices, ranging from ultramafic to felsic rocks. Throughout each of the column chromatography step, 1 mL of cation exchange resin AG50W-X8 (200–400 mesh) was used as the stationary phase and oxalic acid as a chelating agent, was used in addition to the inorganic acids. This method yielded high recoveries of Cr [93 ± 8% (2SD, N = 7)] regardless of the lithology. The total procedural blank of Cr was <0.5 ng. We also developed a double spike-total evaporation-thermal ionization mass spectrometry (DS-TE-TIMS) technique that significantly reduced sample consumption to ∼20 ng of Cr per each measurement of mass-dependent 53Cr/52Cr.
Significance: This study achieved a 2SD external precision of 0.02‰ for the analysis of NIST NBS3112a and of 0.01–0.07‰ for the geological samples. This study enabled high-precision Cr isotope analysis in geological samples with various matrix and Cr compositions using relatively small sample volumes. en-copyright= kn-copyright= en-aut-name=RatnayakeDilan M. en-aut-sei=Ratnayake en-aut-mei=Dilan M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraEizo en-aut-sei=Nakamura en-aut-mei=Eizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=2 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=3 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= en-keyword=Cr isotopes kn-keyword=Cr isotopes en-keyword=DS-TE-TIMS kn-keyword=DS-TE-TIMS en-keyword=Cation exchange resin kn-keyword=Cation exchange resin en-keyword=Low blank kn-keyword=Low blank en-keyword=High precision kn-keyword=High precision END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=6 article-no= start-page=627 end-page=634 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202312 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Abnormal Vaginal Cytology after Total Laparoscopic Hysterectomy in Patients with Cervical Intraepithelial Neoplasia en-subtitle= kn-subtitle= en-abstract= kn-abstract=To explore the incidence of abnormal vaginal cytology after total laparoscopic hysterectomy for the treatment of cervical intraepithelial neoplasia 3, we retrospectively analyzed the medical records of patients treated at NHO Shikoku Cancer Center (Japan) in 2014-2019. The cases of 99 patients who underwent a laparoscopic (n=36) or open (n=63) hysterectomy and postoperative follow-up were examined. Abnormal vaginal cytology was detected in 13.9% (5/36) of the laparoscopic-surgery (LS) group and 14.3% (9/63) of the open-surgery (OS) group. A vaginal biopsy was performed at the physicians’ discretion; one LS patient and six OS patients were diagnosed with vaginal intraepithelial neoplasia. The cumulative incidence of abnormal vaginal cytology at 3 years post-hysterectomy was 21.4% (LS group) and 20.5% (OS group), a nonsignificant difference. A multivariate analysis showed that age > 50 years was the only independent risk factor for abnormal vaginal cytology among the covariates examined including age; body mass index; histories of vaginal delivery, abdominal surgery, and smoking; and surgical approach (hazard ratio 8.11; 95% confidence interval 1.73-37.98; p=0.01). These results suggest that the occurrence of abnormal vaginal cytology after a hysterectomy may not be influenced by the laparoscopic procedure but is associated with older age. en-copyright= kn-copyright= en-aut-name=HibinoYumi en-aut-sei=Hibino en-aut-mei=Yumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Okazawa-SakaiMika en-aut-sei=Okazawa-Sakai en-aut-mei=Mika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokoyamaTakanori en-aut-sei=Yokoyama en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujimotoEtsuko en-aut-sei=Fujimoto en-aut-mei=Etsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkameShinichi en-aut-sei=Okame en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TeramotoNorihiro en-aut-sei=Teramoto en-aut-mei=Norihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakeharaKazuhiro en-aut-sei=Takehara en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=2 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=3 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=4 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=5 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=6 en-affil=Department of Pathology, NHO Shikoku Cancer Center kn-affil= affil-num=7 en-affil=Department of Gynecologic Oncology, NHO Shikoku Cancer Center kn-affil= en-keyword=total laparoscopic hysterectomy kn-keyword=total laparoscopic hysterectomy en-keyword=vaginal intraepithelial neoplasia kn-keyword=vaginal intraepithelial neoplasia en-keyword=cervical intraepithelial neoplasia kn-keyword=cervical intraepithelial neoplasia en-keyword=vaginal cytology kn-keyword=vaginal cytology en-keyword=risk factor kn-keyword=risk factor END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=6 article-no= start-page=567 end-page=575 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202312 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Review of a Series of Surveys on Adverse Reactions to the COVID-19 mRNA-1273 Vaccine at Okayama University en-subtitle= kn-subtitle= en-abstract= kn-abstract=This paper presents the results of a series of surveys conducted from July 2021 to March 2023 to investigate the post-vaccination adverse reactions to the mRNA-1273 (Moderna) vaccine among faculty, staff, and students at Okayama University. These studies complement the official surveys conducted by the Ministry of Health, Labour and Welfare (MHLW) and provide a more representative picture of adverse reactions in the general population including large numbers of healthy young people. Pain, swelling, redness at the injection site, fever, headache, and malaise were the main adverse reactions reported. The proportion of adverse reactions was generally higher after the second vaccination and decreased with each additional vaccination. No statistically significant differences in the adverse reactions were found for males and females and those with/without a history of allergy, but a lower proportion of fever was observed in older participants and those with underlying medical conditions. We also evaluated the association between adverse reactions and antibody titers after the third vaccination and found no significant differences in antibody levels one month after vaccination. This series of studies highlights the importance of conducting surveys in diverse populations to provide a more representative picture of post-vaccination adverse reactions during a pandemic. en-copyright= kn-copyright= en-aut-name=MatsumotoNaomi en-aut-sei=Matsumoto en-aut-mei=Naomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiguchiChigusa en-aut-sei=Higuchi en-aut-mei=Chigusa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyajiChikara en-aut-sei=Miyaji en-aut-mei=Chikara kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MitsuhashiToshiharu en-aut-sei=Mitsuhashi en-aut-mei=Toshiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HagiyaHideharu en-aut-sei=Hagiya en-aut-mei=Hideharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YorifujiTakashi en-aut-sei=Yorifuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Epidemiology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Okayama University Health Service Center kn-affil= affil-num=3 en-affil=Okayama University Health Service Center kn-affil= affil-num=4 en-affil=Center for Innovative Clinical Medicine, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Infectious Diseases, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Epidemiology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Department of Epidemiology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=coronavirus disease 2019 kn-keyword=coronavirus disease 2019 en-keyword=adverse reactions kn-keyword=adverse reactions en-keyword=mRNA vaccine kn-keyword=mRNA vaccine en-keyword=antibody titers kn-keyword=antibody titers en-keyword=young adults kn-keyword=young adults END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=4 article-no= start-page=407 end-page=414 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Usefulness of Force-Controlled Pelvic Stress Radiograph in the Evaluation and Treatment of Fragility Fractures of the Pelvis in Geriatric Patients: A Pilot Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=This study aimed to investigate the usefulness of force-controlled pelvic stress radiographs in the evaluation and treatment of fragility fractures of the pelvis (FFP) using a functional treatment strategy. We conducted a retrospective study of 55 geriatric patients with FFP who underwent pelvic stress radiographs on admission. The differences in the sacral width, pelvic ring width, and medial femoral head width between the radiographs with and without the Sam Sling II M size were defined as Δ sacral width, Δ pelvic ring width, and Δ medial femoral head width, respectively. We used Pearson’s correlation test to assess the relationship between the degree of radiographic instability and the Johns Hopkins highest level of mobility scale (JH-HLM) at 10-days postadmission. Conventional receiver-operating-characteristic curve analysis was used to identify cases requiring surgery using the best cutoff value for radiographic instability. The JH-HLM was significantly correlated with Δ sacral width (r=−0.401, p=0.017), but not with Δ pelvic ring width (r=−0.298, p=0.080) nor with Δ medial femoral head width (r= −0.261, p=0.128). The best cutoff value of Δ sacral width in identifying surgical cases was 10.7 mm (sensitivity 75.0%, specificity 98.0%). Force-controlled pelvic stress radiographs could be helpful in assessing the need for surgery on admission. en-copyright= kn-copyright= en-aut-name=HottaKensuke en-aut-sei=Hotta en-aut-mei=Kensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KobayashiTakaomi en-aut-sei=Kobayashi en-aut-mei=Takaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Amagi Chuo Hospital kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Karatsu Red Cross Hospital kn-affil= en-keyword=fragility fracture of the pelvis kn-keyword=fragility fracture of the pelvis en-keyword=functional treatment strategy kn-keyword=functional treatment strategy en-keyword=Sam Sling kn-keyword=Sam Sling en-keyword=stress radiograph kn-keyword=stress radiograph en-keyword=Johns Hopkins highest level of mobility scale kn-keyword=Johns Hopkins highest level of mobility scale END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=4 article-no= start-page=377 end-page=385 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Disease Progression-Related Markers for Aged Non-Alcoholic Fatty Liver Disease Patients en-subtitle= kn-subtitle= en-abstract= kn-abstract=Liver fibrosis is an important phenomenon in non-alcoholic fatty liver disease (NAFLD) progression. Standard markers reflecting liver fibrosis, including the FIB-4 index, increase with age. This study aimed to identify fibrosis progression-related markers that are diagnostically beneficial even in aged individuals. Serum levels of pro- and anti-inflammatory cytokines were measured by multiple enzyme-linked immunosorbent assay. Two standard NAFLD or fibrosis progression-related markers — the FIB-4 index and APRI score — were analyzed along with cytokine levels to define the best approach to discriminate advanced fibrosis. Ninety-eight NAFLD patients were enrolled: 59 and 39 patients with fibrosis stages 1-2 and 3-4 respectively. In addition to the FIB-4 index and APRI score, the following factors showed significant differences between stages 1-2 and stages 3-4 in a multivariate analysis: platelet counts, IP-10, and RANTES. The fibrosis stage, FIB-4, APRI, PDGF-BB, and RANTES were related to the prognosis. In aged patients, IP-10, GM-CSF, and RANTES differed between stages 1-2 and stages 3-4. FIB-4 and APRI were beneficial for their correlation with fibrosis. However, to stratify either young or elderly advanced fibrosis patients, and to identify patients likely to have a bad outcome, RANTES was the best marker. en-copyright= kn-copyright= en-aut-name=MorimotoKosaku en-aut-sei=Morimoto en-aut-mei=Kosaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeuchiYasuto en-aut-sei=Takeuchi en-aut-mei=Yasuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakakiAkinobu en-aut-sei=Takaki en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WadaNozomu en-aut-sei=Wada en-aut-mei=Nozomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OyamaAtsushi en-aut-sei=Oyama en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AdachiTakuya en-aut-sei=Adachi en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OnishiHideki en-aut-sei=Onishi en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ShirahaHidenori en-aut-sei=Shiraha en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=NAFLD kn-keyword=NAFLD en-keyword=NASH kn-keyword=NASH en-keyword=liver fibrosis kn-keyword=liver fibrosis en-keyword=chemokine kn-keyword=chemokine en-keyword=FIB-4 kn-keyword=FIB-4 END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=4 article-no= start-page=359 end-page=364 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202308 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in TRPV1 Receptor, CGRP, and BDNF Expression in Rat Dorsal Root Ganglion with Resiniferatoxin-Induced Neuropathic Pain: Modulation by Pulsed Radiofrequency Applied to the Sciatic Nerve en-subtitle= kn-subtitle= en-abstract= kn-abstract=Pulsed radiofrequency (PRF) is a safe method of treating neuropathic pain by generating intermittent electric fields at the needle tip. Resiniferatoxin (RTX) is an ultrapotent agonist of transient receptor potential vanilloid subtype-1 (TRPV1) receptors. We investigated the mechanism of PRF using a rat model of RTX-induced neuropathic pain. After administering RTX intraperitoneally, PRF was applied to the right sciatic nerve. We observed the changes in TRPV1, calcitonin gene-related peptide (CGRP), and brain-derived neurotrophic factor (BDNF) in the dorsal root ganglia by western blotting. Expressions of TRPV1 and CGRP were significantly lower in the contralateral (RTX-treated, PRF-untreated) tissue than in control rats (p<0.0001 and p<0.0001, respectively) and the ipsilateral tissues (p<0.0001 and p<0.0001, respectively). BDNF levels were significantly higher in the contralateral tissues than in the control rats (p<0.0001) and the ipsilateral tissues (p<0.0001). These results suggest that, while TRPV1 and CGRP are decreased by RTX-induced neuronal damage, increased BDNF levels result in pain development. PRF may promote recovery from neuronal damage with concomitant restoration of TRPV1 and CGRP, and exert its analgesic effect by reversing BDNF increase. Further research is required to understand the role of TRPV1 and CGRP restoration in improving mechanical allodynia. en-copyright= kn-copyright= en-aut-name=KoshidaTomohiro en-aut-sei=Koshida en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MarutaToyoaki en-aut-sei=Maruta en-aut-mei=Toyoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanakaNobuhiko en-aut-sei=Tanaka en-aut-mei=Nobuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HidakaKotaro en-aut-sei=Hidaka en-aut-mei=Kotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KurogiMio en-aut-sei=Kurogi en-aut-mei=Mio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NemotoTakayuki en-aut-sei=Nemoto en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YanagitaToshihiko en-aut-sei=Yanagita en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakeyaRyu en-aut-sei=Takeya en-aut-mei=Ryu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TsuneyoshiIsao en-aut-sei=Tsuneyoshi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=2 en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=3 en-affil=Tanaka homecare clinic kn-affil= affil-num=4 en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=5 en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=6 en-affil=Department of Pharmacology, Faculty of Medicine, Fukuoka University kn-affil= affil-num=7 en-affil=Department of Clinical Pharmacology, School of Nursing, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=8 en-affil=Department of Pharmacology, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=9 en-affil=Department of Anesthesiology and Pain Clinic, Faculty of Medicine, University of Miyazaki kn-affil= en-keyword=pulsed radiofrequency kn-keyword=pulsed radiofrequency en-keyword=resiniferatoxin kn-keyword=resiniferatoxin en-keyword=transient receptor potential vanilloid subtype-1 (TRPV1) kn-keyword=transient receptor potential vanilloid subtype-1 (TRPV1) en-keyword=calcitonin gene-related peptide (CGRP) kn-keyword=calcitonin gene-related peptide (CGRP) en-keyword=brain-derived neurotrophic factor (BDNF) kn-keyword=brain-derived neurotrophic factor (BDNF) END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=335 end-page=340 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Case of High-Grade Glioma in an Eloquent Area Treated with Awake Craniotomy in an 85-year-old Patient en-subtitle= kn-subtitle= en-abstract= kn-abstract=An 85-year-old woman presented with aphasia due to an occupying lesion in the left frontal lobe near the language area. Complete resection of the contrast-enhancing lesion was performed under awake conditions. The pathological diagnosis was anaplastic astrocytoma, and postoperative radiochemotherapy was administered. Awake surgery is a useful technique to reduce postoperative neurological sequelae and to maximize surgical resection. Although the patient was elderly, which is generally considered high risk, she did not have any severe neurological deficits and had a good outcome. Even in the extreme elderly, awake surgery can be useful for gliomas in language cortices. en-copyright= kn-copyright= en-aut-name=FujiiKentaro en-aut-sei=Fujii en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiranoShuichiro en-aut-sei=Hirano en-aut-mei=Shuichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KurozumiKazuhiko en-aut-sei=Kurozumi en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DateIsao en-aut-sei=Date en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Neurological Surgery, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Neurological Surgery, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Neurological Surgery, Hamamatsu University School of Medicine, University Hospital kn-affil= affil-num=4 en-affil=Department of Neurological Surgery, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=awake surgery kn-keyword=awake surgery en-keyword=high-grade glioma kn-keyword=high-grade glioma en-keyword=eloquent area kn-keyword=eloquent area en-keyword=elderly patient kn-keyword=elderly patient END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=301 end-page=309 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Associations between Comorbidities and Acute Exacerbation of Interstitial Lung Disease after Primary Lung Cancer Surgery en-subtitle= kn-subtitle= en-abstract= kn-abstract=Acute exacerbation (AE) of interstitial lung disease (ILD) is a severe complication of lung resection in lung cancer patients with ILD (LC-ILD). This study aimed to assess the predictive value of comorbidities other than ILD for postoperative AE in patients with LC-ILD. We retrospectively evaluated 68 patients with LC-ILD who had undergone lung resection. We classified them into two groups: those who had developed postoperative AE within 30 days after resection and those who had not. We analyzed patient characteristics, high-resolution computed tomography findings, clinical data, pulmonary function, and intraoperative data. The incidence of postoperative AEs was 11.8%. In univariate analysis, performance status (PS), honeycombing, forced vital capacity (FVC), and high hemoglobin A1c (HbA1c) levels without comorbidities were significantly associated with postoperative AE. Patients were divided into two groups according to cutoff levels of those four variables as determined by receiver operating characteristic curves, revealing that the rates of patients without postoperative AE differed significantly between groups. The present results suggested that preoperative comorbidities other than ILD were not risk factors for postoperative AE in patients with LC-ILD. However, a high preoperative HbA1c level, poor PS, low FVC, and honeycombing may be associated with postoperative AE of LC-ILD. en-copyright= kn-copyright= en-aut-name=KatoTakahide en-aut-sei=Kato en-aut-mei=Takahide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyoshiSeigo en-aut-sei=Miyoshi en-aut-mei=Seigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HamadaChizuru en-aut-sei=Hamada en-aut-mei=Chizuru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SanoYoshifumi en-aut-sei=Sano en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NogamiNaoyuki en-aut-sei=Nogami en-aut-mei=Naoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamaguchiOsamu en-aut-sei=Yamaguchi en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HamaguchiNaohiko en-aut-sei=Hamaguchi en-aut-mei=Naohiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Cardiology, Pulmonology, Hypertension and Nephrology, Ehime University Graduate School of Medicine kn-affil= affil-num=2 en-affil=Department of Cardiology, Pulmonology, Hypertension and Nephrology, Ehime University Graduate School of Medicine kn-affil= affil-num=3 en-affil=Department of Cardiology, Pulmonology, Hypertension and Nephrology, Ehime University Graduate School of Medicine kn-affil= affil-num=4 en-affil=Department of Cardiovascular and Thoracic Surgery, Ehime University Graduate School of Medicine kn-affil= affil-num=5 en-affil=Department of Community Medicine, Pulmonology and Cardiology, Ehime University Graduate School of Medicine kn-affil= affil-num=6 en-affil=Department of Cardiology, Pulmonology, Hypertension and Nephrology, Ehime University Graduate School of Medicine kn-affil= affil-num=7 en-affil=Department of Cardiology, Pulmonology, Hypertension and Nephrology, Ehime University Graduate School of Medicine kn-affil= en-keyword=lung cancer kn-keyword=lung cancer en-keyword=interstitial lung disease kn-keyword=interstitial lung disease en-keyword=acute exacerbation kn-keyword=acute exacerbation en-keyword=comorbidity kn-keyword=comorbidity END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=281 end-page=290 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Impact of Immediate Breast Reconstruction on Survival of Breast Cancer Patients: A Single-Center Observational Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Although immediate breast reconstruction following mastectomy has become increasingly common, its oncological safety has been debated. We enrolled patients with breast cancer who underwent surgery at Okayama University Hospital between 2007 and 2013. The primary outcome was relapse-free survival (RFS). Secondary outcomes were overall survival and the duration from the surgery to the initiation of adjuvant chemotherapy. We divided into immediate breast reconstruction, mastectomy alone, and breast conservative surgery groups. Outcomes were compared using Cox’s regression analysis. A total of 614 patients were included (reconstruction: 125, mastectomy: 128, breast conservative surgery: 361). The median follow-up duration was 79.0±31.9 months. The immediate-reconstruction patients were younger, had more lymph node metastases, and more often received postoperative chemotherapy. The RFS was better after the breast conservative surgery compared to after reconstruction (hazard ratio 0.33, 95% confidence interval: 0.144-0.763). The proportion of local recurrence was highest in the reconstruction group. No patients in the reconstruction group underwent postoperative radiation therapy. However, reconstruction did not affect overall survival or the time to the initiation of adjuvant chemotherapy. Surgeons should explain the risks of breast reconstruction to their patients preoperatively. Careful long-term follow-up is required after such procedures. en-copyright= kn-copyright= en-aut-name=MukaiYuko en-aut-sei=Mukai en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TairaNaruto en-aut-sei=Taira en-aut-mei=Naruto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KajiwaraYukiko en-aut-sei=Kajiwara en-aut-mei=Yukiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IwamotoTakayuki en-aut-sei=Iwamoto en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitaguchiYohei en-aut-sei=Kitaguchi en-aut-mei=Yohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SaigaMiho en-aut-sei=Saiga en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WatanabeSatoko en-aut-sei=Watanabe en-aut-mei=Satoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ShienTadahiko en-aut-sei=Shien en-aut-mei=Tadahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=DoiharaHiroyoshi en-aut-sei=Doihara en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KimataYoshihiro en-aut-sei=Kimata en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Breast and Thyroid Surgery, Kawasaki Medical School Hospital kn-affil= affil-num=3 en-affil=Department of Breast Surgery, Hiroshima Citizens Hospital kn-affil= affil-num=4 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=9 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=10 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=immediate breast reconstruction kn-keyword=immediate breast reconstruction en-keyword=oncological safety kn-keyword=oncological safety en-keyword=local recurrence kn-keyword=local recurrence en-keyword=postoperative radiation therapy kn-keyword=postoperative radiation therapy en-keyword=time to initiation of adjuvant chemotherapy kn-keyword=time to initiation of adjuvant chemotherapy END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=243 end-page=254 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Brown Adipose Tissue PPARγ Is Required for the Insulin-Sensitizing Action of Thiazolidinediones en-subtitle= kn-subtitle= en-abstract= kn-abstract=Brown adipose tissue (BAT) plays a critical role in metabolic homeostasis. BAT dysfunction is associated with the development of obesity through an imbalance between energy expenditure and energy intake. The nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) is the master regulator of adipogenesis. However, the roles of PPARγ and thiazolidinediones (TZDs) in the regulation of BAT metabolism remain unclear. TZDs, which are selective PPARγ activators, improve systemic insulin resistance in animals and humans. In the present study, we generated brown adipocyte-specific PPARγ-deficient mice (BATγKO) to examine the in vivo roles of PPARγ and TZDs in BAT metabolism. In electron microscopic examinations, brown adipocyte-specific PPARγ deletion promoted severe whitening of brown fat and morphological alteration of mitochondria. Brown adipocyte-specific PPARγ deletion also reduced mRNA expression of BAT-selective genes. Although there was no difference in energy expenditure between control and BATγKO mice in calorimetry, norepinephrine-induced thermogenesis was impaired in BATγKO mice. Moreover, pioglitazone treatment improved diet-induced insulin resistance in the control mice but not in the BATγKO mice. These findings suggest that BAT PPARγ is necessary for the maintenance of brown adipocyte function and for the insulin-sensitizing action of TZDs. en-copyright= kn-copyright= en-aut-name=ShibataYusuke en-aut-sei=Shibata en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EguchiJun en-aut-sei=Eguchi en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=PPARγ kn-keyword=PPARγ en-keyword=brown adipose tissue kn-keyword=brown adipose tissue en-keyword=thiazolidinediones kn-keyword=thiazolidinediones END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=2 article-no= start-page=139 end-page=145 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202304 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Prolonged Sedentary Bouts Are Critically Involved in All-Cause Mortality in Patients on Chronic Hemodialysis: A Prospective Cohort Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=We investigated the link between prolonged sedentary bouts and all-cause mortality in patients on chronic hemodialysis, using a prospective cohort. A total of 104 outpatients on chronic hemodialysis from 2013 to 2019, aged 71.4±11.4 years, were enrolled. Prolonged sedentary bouts (≥ 30 min and ≥60 min) (min and bouts) and relative prolonged sedentary bouts (≥ 30 min and ≥ 60 min) (%) on the patients’ non-hemodialysis days were measured by a tri-accelerometer, and we also analyzed the patients’ clinical parameters. The relationship between prolonged sedentary bouts and all-cause mortality was evaluated by a survival analysis and the Cox proportional hazard model. Thirty-five patients died during the follow-up period. A Kaplan-Meier analysis detected significant differences in the survival rate between two groups stratified by the median for all prolonged sedentary-bout parameters. After the adjustment for confounding factors, all of the prolonged sedentary-bout parameters were determinant factors for all-cause mortality. These results indicate that prolonged sedentary bouts on non-hemodialysis days were closely related to all-cause mortality in the patients on hemodialysis. en-copyright= kn-copyright= en-aut-name=NamioKeiichi en-aut-sei=Namio en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KondoTakashi en-aut-sei=Kondo en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyatakeNobuyuki en-aut-sei=Miyatake en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HishiiShuhei en-aut-sei=Hishii en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishiHiroyuki en-aut-sei=Nishi en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KatayamaAkihiko en-aut-sei=Katayama en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=UjikeKazuhiro en-aut-sei=Ujike en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SuzukiHiromi en-aut-sei=Suzuki en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KoumotoKiichi en-aut-sei=Koumoto en-aut-mei=Kiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University kn-affil= affil-num=2 en-affil=Innoshima General Hospital kn-affil= affil-num=3 en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University kn-affil= affil-num=4 en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University kn-affil= affil-num=5 en-affil=Innoshima General Hospital kn-affil= affil-num=6 en-affil=Faculty of Social Studies, Shikokugakuin University kn-affil= affil-num=7 en-affil=Innoshima General Hospital kn-affil= affil-num=8 en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University kn-affil= affil-num=9 en-affil=Innoshima General Hospital kn-affil= en-keyword=sedentary bout kn-keyword=sedentary bout en-keyword=mortality kn-keyword=mortality en-keyword=hemodialysis kn-keyword=hemodialysis en-keyword=survival analysis kn-keyword=survival analysis END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue=4 article-no= start-page=669 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association between Urinary Creatinine Excretion and Hypothyroidism in Patients with Chronic Kidney Disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=While hypothyroidism increases serum creatinine (Cr) levels, it is uncertain whether the elevation is mediated via a decline in the glomerular filtration rate (GFR) or the reflection of enhanced Cr production from the muscles or both. In the present study, we explored an association between urinary Cr excretion rate (CER) and hypothyroidism. A total of 553 patients with chronic kidney disease were enrolled in a cross-sectional study. Multiple linear regression analysis was performed to explore the association between hypothyroidism and urinary CER. The mean urinary CER was 1.01 +/- 0.38 g/day and 121 patients (22%) had hypothyroidism. The multiple linear regression analysis revealed explanatory variables with urinary CER, including age, sex, body mass index, 24 h Cr clearance (24hrCcr), and albumin while hypothyroidism was not considered an independent explanatory variable. In addition, scatter plot analysis with regression fit line representing the association between estimated GFR calculated using s-Cr (eGFRcre) and 24hrCcr revealed that eGFRcre and 24hrCcr had strong correlations with each other in hypothyroid patients as well as euthyroid patients. Collectively, hypothyroidism was not considered an independent explanatory variable for urinary CER in the present study and eGFRcre is a useful marker to evaluate kidney function regardless of the presence of hypothyroidism. en-copyright= kn-copyright= en-aut-name=Matsuoka-UchiyamaNatsumi en-aut-sei=Matsuoka-Uchiyama en-aut-mei=Natsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujiKenji en-aut-sei=Tsuji en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakahashiKensaku en-aut-sei=Takahashi en-aut-mei=Kensaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FukushimaKazuhiko en-aut-sei=Fukushima en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakeuchiHidemi en-aut-sei=Takeuchi en-aut-mei=Hidemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KitamuraShinji en-aut-sei=Kitamura en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=InagakiKenichi en-aut-sei=Inagaki en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UchidaHaruhito A. A. en-aut-sei=Uchida en-aut-mei=Haruhito A. A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Chronic Kidney Disease and Cardiovascular Disease, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=hypothyroidism kn-keyword=hypothyroidism en-keyword=kidney function kn-keyword=kidney function en-keyword=urinary creatinine excretion kn-keyword=urinary creatinine excretion END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=1 article-no= start-page=71 end-page=74 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ipsilateral Periprosthetic Fractures above and below the Knee Associated with Navigation Tracker Pin and Bone Fragility en-subtitle= kn-subtitle= en-abstract= kn-abstract=We report a case of ipsilateral periprosthetic fractures above and below the knee that occurred at different times due to navigation tracker pin and bone fragility. A 66-year-old Japanese woman with rheumatoid arthritis (RA) underwent a total knee arthroplasty. Four months post-surgery, a periprosthetic fracture above the knee at the navigation pin hole was detected. She underwent osteosynthesis and could walk independently, but she developed an ipsilateral tibial component fracture. Conservative treatment with a splint was followed by bone union. Patients with RA treated with oral steroids tend to develop ipsilateral periprosthetic fractures around the knee due to bone fragility. en-copyright= kn-copyright= en-aut-name=YamakawaYasuaki en-aut-sei=Yamakawa en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KamatsukiYusuke en-aut-sei=Kamatsuki en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NodaTomoyuki en-aut-sei=Noda en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KureMiho en-aut-sei=Kure en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyazawaShinichi en-aut-sei=Miyazawa en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Orthopedic Surgery, Kochi Health Sciences Center kn-affil= affil-num=2 en-affil=Department of Orthopedic Surgery, Kochi Health Sciences Center kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= en-keyword=periprosthetic fracture kn-keyword=periprosthetic fracture en-keyword=total knee arthroplasty kn-keyword=total knee arthroplasty en-keyword=navigation system kn-keyword=navigation system en-keyword=bone fragility kn-keyword=bone fragility END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=4 article-no= start-page=430 end-page=442 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20221213 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Endoscopic ablation therapy for the pancreatic neoplasms en-subtitle= kn-subtitle= en-abstract= kn-abstract=Recently, endoscopic ultrasound (EUS)-guided ablation therapy has been reported as a less invasive therapy for patients with pancreatic neoplasms. Some ablation techniques, including injective ablation (using ethanol or other ablative agents), radiofrequency ablation (RFA), photodynamic therapy, and laser ablation, have been described in the literature. Among these, injective ablation and RFA are more frequently used for treating pancreatic neoplasms. Few studies have evaluated the effectiveness of EUS-guided ethanol ablation (EUS-EA) for potentially malignant solid neoplasms (neuroendocrine neoplasms or solid pseudopapillary neoplasms) and have reported a complete response (CR) rate of 60-80%. In addition, the CR rate after EUS-RFA for these lesions has been reported to be 55-100%, with no additional procedure-related adverse events (AEs). Regarding the amelioration of the symptoms of an insulinoma, the success rates of both the therapies were found to be excellent. Regarding complete tumor ablation, EUS-RFA appeared to be superior to EUS-EA. Although EUS-RFA has been reported as a safe treatment for pancreatic cancers, its effectiveness remains inadequate. Some studies have examined the effectiveness of EUS-guided injection ablation therapy for pancreatic cystic neoplasms (PCNs) and have reported CR rates that range from 35% to 79%. Alcohol-free chemotherapeutic agent ablation appears to be effective, with a low risk of AEs. However, studies on the effectiveness of EUS-RFA for PCNs are limited. In the future, EUS-guided ablation therapy could become a more widely used approach for potentially malignant and malignant pancreatic lesions. en-copyright= kn-copyright= en-aut-name=MatsumotoKazuyuki en-aut-sei=Matsumoto en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KatoHironari en-aut-sei=Kato en-aut-mei=Hironari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Gastroenterology and Hepatology, Okayama University Hospital kn-affil= en-keyword=ablation techniques kn-keyword=ablation techniques en-keyword=endoscopic ultrasonography kn-keyword=endoscopic ultrasonography en-keyword=ethanol kn-keyword=ethanol en-keyword=pancreatic neoplasms kn-keyword=pancreatic neoplasms en-keyword=radiofrequency ablation kn-keyword=radiofrequency ablation END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=6 article-no= start-page=749 end-page=754 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Salvage Surgery for Symptomatic Recurrence of Retro-Odontoid Pseudotumor after a C1 Laminectomy en-subtitle= kn-subtitle= en-abstract= kn-abstract=We provide the first report of successful salvage surgery for a post-C1 laminectomy symptomatic recurrence of a retro-odontoid pseudotumor (ROP) that caused myelopathy. The 72-year-old Japanese woman presented with an ROP causing symptomatic cervical myelopathy. With ultrasonography support, we performed the enucleation of the ROP via a transdural approach and fusion surgery for the recurrence of the mass. At the final observation 2-year post-surgery, MRI demonstrated the mass’s regression and spinal cord decompression, and the patient’s symptoms had improved. Our strategy is an effective option for a symptomatic recurrence of ROP. en-copyright= kn-copyright= en-aut-name=TakamiMasanari en-aut-sei=Takami en-aut-mei=Masanari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YukawaYasutsugu en-aut-sei=Yukawa en-aut-mei=Yasutsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NodaYusuke en-aut-sei=Noda en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshidaMunehito en-aut-sei=Yoshida en-aut-mei=Munehito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamadaHiroshi en-aut-sei=Yamada en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Wakayama Medical University kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Wakayama Medical University kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Wakayama Rosai Hospital kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Wakayama Medical University kn-affil= affil-num=5 en-affil=Department of Orthopaedic Surgery, Wakayama Medical University kn-affil= en-keyword=recurrent retro-odontoid pseudotumor kn-keyword=recurrent retro-odontoid pseudotumor en-keyword=salvage surgery kn-keyword=salvage surgery en-keyword=transdural resection kn-keyword=transdural resection en-keyword=C1 laminectomy kn-keyword=C1 laminectomy en-keyword=ultra-sonography kn-keyword=ultra-sonography END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=5 article-no= start-page=565 end-page=575 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Simple and Descriptive Assessment of Morphology Based on the Horizontal Plane of the Pediatric Head and Creation of a Normative Database in Japanese Children 6 Years Old and under: Horizontal Vector Analysis en-subtitle= kn-subtitle= en-abstract= kn-abstract=We herein introduce horizontal vector analysis, a simple method for assessing cranial morphology based on measurement of the head’s horizontal plane, and use this method to establish normal cranial morphology in Japanese children Computed tomography scans taken in 2010-2019 in healthy Japanese children aged ≤ 6 years. The two measurement planes were parallel to the orbitomeatal plane: namely, a plane passing through the dorsum sellae (DS) and the plane superior to that with the maximal area (Max plane). A protractor was used to circumferentially measure the lengths from the central point to the outer surface of the skull. A total of 487 images were extracted. The distances between the DS and Max planes were consistently almost 30 mm for each age group, so we fixed the Max plane as the plane 30 mm superior to the DS plane. Finally, we established datasets of normal values for each age group and sex. Using these norms, perioperative evaluation of various cranial deformities could be performed more easily and circumstantially. en-copyright= kn-copyright= en-aut-name=KomagoeSho en-aut-sei=Komagoe en-aut-mei=Sho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SenooTakaya en-aut-sei=Senoo en-aut-mei=Takaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShiraishiYoshinori en-aut-sei=Shiraishi en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsumotoHiroshi en-aut-sei=Matsumoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimataYoshihiro en-aut-sei=Kimata en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=craniofacial surgery kn-keyword=craniofacial surgery en-keyword=craniosynostoses kn-keyword=craniosynostoses en-keyword=horizontal plane kn-keyword=horizontal plane en-keyword=Japanese children kn-keyword=Japanese children en-keyword=reference values kn-keyword=reference values END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=5 article-no= start-page=489 end-page=502 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Current Insights into Mesenchymal Signatures in Glioblastoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=Glioblastoma (GBM) is a fatal primary malignant brain tumor in adults. Despite decades of research, the prognosis for GBM patients is still disappointing. One major reason for the intense therapeutic resistance of GBM is inter- and intra-tumor heterogeneity. GBM-intrinsic transcriptional profiling has suggested the presence of at least three subtypes of GBM: the proneural, classic, and mesenchymal subtypes. The mesenchymal subtype is the most aggressive, and patients with the mesenchymal subtype of primary and recurrent tumors tend to have a worse prognosis compared with patients with the other subtypes. Furthermore, GBM can shift from other subtypes to the mesenchymal subtype over the course of disease progression or recurrence. This phenotypic transition is driven by diverse tumor-intrinsic molecular mechanisms or microenvironmental factors. Thus, better understanding of the plastic nature of mesenchymal transition in GBM is pivotal to developing new therapeutic strategies. In this review, we provide a comprehensive overview of the current understanding of the elements involved in the mesenchymal transition of GBM and discuss future perspectives. en-copyright= kn-copyright= en-aut-name=MatsumotoYuji en-aut-sei=Matsumoto en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IchikawaTomotsugu en-aut-sei=Ichikawa en-aut-mei=Tomotsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KurozumiKazuhiko en-aut-sei=Kurozumi en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DateIsao en-aut-sei=Date en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Neurological Surgery, Kagawa Prefectural Central Hospital kn-affil= affil-num=3 en-affil=Department of Neurosurgery, Hamamatsu University Hospital kn-affil= affil-num=4 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=glioma kn-keyword=glioma en-keyword=glioblastoma kn-keyword=glioblastoma en-keyword=mesenchymal subtype kn-keyword=mesenchymal subtype en-keyword=mesenchymal transition kn-keyword=mesenchymal transition en-keyword=heterogeneity kn-keyword=heterogeneity END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=1 article-no= start-page=14999 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The association between hypothyroidism and proteinuria in patients with chronic kidney disease: a cross-sectional study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hypothyroidism is known to be correlated with kidney function and nephrotic range proteinuria. However, it is uncertain whether non-nephrotic proteinuria is associated with hypothyroidism. This study aimed to evaluate the association of proteinuria and hypothyroidism in chronic kidney disease (CKD) patients. We conducted a cross-sectional study composed of 421 CKD patients in a single hospital with measurements of 24-h urine protein excretion (UP) and thyroid function tests. Spearman correlation analysis revealed that 24-h Cr clearance (24hrCcr) was positively (r = 0.273, p < 0.001) and UP was negatively (r = - 0.207, p < 0.001) correlated with free triiodothyronine. Frequency distribution analysis stratified by CKD stage and UP for hypothyroidism revealed that the prevalence of hypothyroidism was higher among participants with higher CKD stage and nephrotic range proteinuria. Multivariate logistic regression analysis revealed that 24hrCcr and UP were significantly correlated with hypothyroidism (24hrCcr/10 mL/min decrease: odds ratio [OR], 1.29; 95% confidence interval [CI], 1.18-1.41; UP/1 g increase: OR, 1.10; 95% CI, 1.03-1.17). In addition, nephrotic range proteinuria, but not moderate UP (UP: 1.5-3.49 g/day), was significantly correlated with hypothyroidism compared to UP < 0.5 g/day. In summary, decreased kidney function and nephrotic range proteinuria, not non-nephrotic proteinuria, are independently associated with the hypothyroidism. en-copyright= kn-copyright= en-aut-name=Matsuoka-UchiyamaNatsumi en-aut-sei=Matsuoka-Uchiyama en-aut-mei=Natsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujiKenji en-aut-sei=Tsuji en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SangYizhen en-aut-sei=Sang en-aut-mei=Yizhen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakahashiKensaku en-aut-sei=Takahashi en-aut-mei=Kensaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FukushimaKazuhiko en-aut-sei=Fukushima en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakeuchiHidemi en-aut-sei=Takeuchi en-aut-mei=Hidemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=InagakiKenichi en-aut-sei=Inagaki en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=UchidaHaruhito A. en-aut-sei=Uchida en-aut-mei=Haruhito A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KitamuraShinji en-aut-sei=Kitamura en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SugiyamaHitoshi en-aut-sei=Sugiyama en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Chronic Kidney Disease and Cardiovascular Disease, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Human Resource Development of Dialysis Therapy for Kidney Disease, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=11 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=4 article-no= start-page=439 end-page=446 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202208 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Social Capital and Post-traumatic Stress Disorder among Heavy Rainfall and Flood Victims in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=This study examined the relationship between cognitive/structural social capital and post-traumatic stress disorder (PTSD) among victims of heavy rain and flood. Participants were individuals aged≥18 years affected by the July 2018 heavy rainfall in the cities of Kurashiki and Soja, Japan, and living in temporary housing. We distributed five copies of a questionnaire to 1,991 households and received responses from 1,927 individuals (907 men, 1,008 women, 12 respondents of unspecified sex) in 1,029 households (51.7%). We estimated odds ratios (ORs) and 95% confidence intervals (CIs) for associations between high (vs. low) social capital and PTSD or other outcomes. After covariate adjustment, the odds of having PTSD were lower in participants with high cognitive social capital than those with low cognitive social capital (OR=0.346, 95%CI: 0.263-0.456). Elderly women with higher structural social capital tended to have lower PTSD odds than those with lower structural social capital (OR=0.671, 95%CI: 0.431-1.046). The opposite pattern was observed for elderly men (OR=1.315, 95%CI: 0.792-2.183). Cognitive social capital is a protective factor that may reduce PTSD or promote a favorable PTSD prognosis after heavy rainfall and flood events. The associations between structural social capital and PTSD differ by age and sex. en-copyright= kn-copyright= en-aut-name=MiyajiChikara en-aut-sei=Miyaji en-aut-mei=Chikara kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NoguchiMasayuki en-aut-sei=Noguchi en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkazakiTsubasa en-aut-sei=Okazaki en-aut-mei=Tsubasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SatoShunsuke en-aut-sei=Sato en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YorifujiTakashi en-aut-sei=Yorifuji en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=4 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=5 en-affil=Okayama Prefectural Mental Health and Welfare Center kn-affil= affil-num=6 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=social capital kn-keyword=social capital en-keyword=post-traumatic stress disorder kn-keyword=post-traumatic stress disorder en-keyword=disaster kn-keyword=disaster en-keyword=flooding kn-keyword=flooding END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=4 article-no= start-page=429 end-page=437 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202208 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Association between Preoperative Blood Pressure Elevations and Postoperative Adverse Outcomes after Non-cardiac Surgery: A Single-center Retrospective Observational Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Blood pressure (BP) often rises before surgery. This study investigated whether BP elevation immediately before surgery was associated with adverse outcomes. Medical records of 11,732 patients (average age: 61 years; male: 47.4%) who underwent non-cardiac elective inpatient surgery under general anesthesia at Kagawa University Hospital between January 2011 and June 2019 were reviewed. Differences between the first BP values measured on the day before surgery and the first BP values in the operating room were defined as Δ systolic BP (ΔSBP) and Δ diastolic BP (ΔDBP). The relationships between ΔSBP/ΔDBP and 30-day mortality, 30-day readmission, and over-the-standard length of hospital stay (OSLOS) were assessed. OSLOS was defined as a hospital stay longer than mean+2 standard deviations and was calculated using the Japanese Diagnosis Procedure Combination data. In univariate analysis, the differences in ΔSBP and ΔDBP between the OSLOS and standard LOS groups were both 2 mmHg. In multivariate logistic regression analysis, only ΔDBP was associated with OSLOS. The adjusted odds ratio (95% confidence interval) for the largest quartile was 1.31 (1.02-1.69) (p<0.05). ΔDBP was associated with OSLOS; however, there may be little need to worry about large ΔSBPs and ΔDBPs in clinical practice. en-copyright= kn-copyright= en-aut-name=YamadoriYusuke en-aut-sei=Yamadori en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiraoTomohiro en-aut-sei=Hirao en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Nlandu R. Ngatu en-aut-sei=Nlandu R. Ngatu en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KandaKanae en-aut-sei=Kanda en-aut-mei=Kanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=Syed Mahfuz Al Hasan en-aut-sei=Syed Mahfuz Al Hasan en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiAkitsu en-aut-sei=Murakami en-aut-mei=Akitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MashimaYukinori en-aut-sei=Mashima en-aut-mei=Yukinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ShirakamiGotaro en-aut-sei=Shirakami en-aut-mei=Gotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Anesthesiology, Faculty of Medicine, Kagawa University kn-affil= affil-num=2 en-affil=Department of Public Health, Faculty of Medicine, Kagawa University kn-affil= affil-num=3 en-affil=Department of Public Health, Faculty of Medicine, Kagawa University kn-affil= affil-num=4 en-affil=Department of Public Health, Faculty of Medicine, Kagawa University kn-affil= affil-num=5 en-affil=Department of Public Health, Faculty of Medicine, Kagawa University kn-affil= affil-num=6 en-affil=Department of Anesthesiology, Faculty of Medicine, Kagawa University kn-affil= affil-num=7 en-affil=Department of Public Health, Faculty of Medicine, Kagawa University kn-affil= affil-num=8 en-affil=Department of Anesthesiology, Faculty of Medicine, Kagawa University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=4 article-no= start-page=359 end-page=371 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202208 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Therapeutic Approaches Targeting miRNA in Systemic Lupus Erythematosus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Systemic lupus erythematosus (SLE) is a potentially fatal systemic autoimmune disease, and its etiology involves both genetic and environmental factors such as sex hormone imbalance, genetic predisposition, epigenetic regulation, and immunological factors. Dysregulation of microRNA (miRNA) is suggested to be one of the epigenetic factors in SLE. miRNA is a 22-nucleotide single-stranded noncoding RNA that contributes to post-transcriptional modulation of gene expression. miRNA targeting therapy has been suggested to be useful for the treatment of cancers and other diseases. Gene knockout and miRNA targeting therapy have been demonstrated to improve SLE disease activity in mice. However, these approaches have not yet reached the level of clinical application. miRNA targeting therapy is limited by the fact that each miRNA has multiple targets. In addition, the expression of certain miRNAs may differ among cell tissues within a single SLE patient. This limitation can be overcome by targeted delivery and chemical modifications. In the future, further research into miRNA chemical modifications and delivery systems will help us develop novel therapeutic agents for SLE. en-copyright= kn-copyright= en-aut-name=Hiramatsu-AsanoSumie en-aut-sei=Hiramatsu-Asano en-aut-mei=Sumie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=systemic lupus erythematosus kn-keyword=systemic lupus erythematosus en-keyword=miRNA kn-keyword=miRNA en-keyword=miRNA targeting therapy kn-keyword=miRNA targeting therapy END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=1 article-no= start-page=128 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Verrucous carcinoma of the esophagus with complete response after chemoradiotherapy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background : Verrucous carcinoma of the esophagus (VCE) is a rare tumor that is difficult to diagnose. In most cases, biopsies show nonspecific inflammatory and hyperkeratotic changes and do not show malignant findings. Most VCEs are slowly growing, locally advanced tumors with few metastases. Treatments for VCE are the same as for normal esophageal cancer, involving combined chemotherapy, surgical resection, and radiation therapy. However, it has been reported that VCE has a poor response to radiation or chemoradiotherapy (CRT). A case of VCE with complete response (CR) after CRT is presented.
Case presentation : A 70-year-old man was found to have white, irregular esophageal mucosa 4 years earlier. He had been followed up as an outpatient as having candidal esophagitis. However, his tumor grew gradually, and biopsy was performed by endoscopic mucosal resection (EMR). He was finally diagnosed with VCE. He had no metastases to distant organs, but some lymph node metastases were suspected. The tumor invaded his left bronchus. The esophagostomy and gastrostomy were constructed as emergent procedures. The patient then underwent definitive CRT. 4 weeks after the end of CRT, two-stage esophagectomy was performed. First, he underwent esophagectomy with thoracic lymph node dissection. A latissimus dorsi flap was patched to the bronchus after primary suture of the hole. 6 weeks later, reconstruction of the gastric tube was performed through the antethoracic route. The pathological findings showed CR to CRT, with no proliferative cancer cells in the specimen. The patient has had no recurrence for three and half years after the resection.
Conclusions : We presented a locally advanced VCE that achieved CR to CRT. In cases that have some difficulty for local resection, CRT might be an appropriate treatment for VCE. en-copyright= kn-copyright= en-aut-name=HashimotoMasashi en-aut-sei=Hashimoto en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShirakawaYasuhiro en-aut-sei=Shirakawa en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanabeShunsuke en-aut-sei=Tanabe en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MaedaNaoaki en-aut-sei=Maeda en-aut-mei=Naoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakuramaKazufumi en-aut-sei=Sakurama en-aut-mei=Kazufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NomaKazuhiro en-aut-sei=Noma en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Esophagectomy kn-keyword=Esophagectomy en-keyword=Verrucous carcinoma kn-keyword=Verrucous carcinoma en-keyword=Esophageal squamous cell carcinoma kn-keyword=Esophageal squamous cell carcinoma END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=3 article-no= start-page=349 end-page=354 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202206 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bilateral Blunt Ocular Trauma Caused by an Exercise Resistance Band during Muscle Building Exercise for Swimming en-subtitle= kn-subtitle= en-abstract= kn-abstract=An 18-year-old male high school student presented to our clinic with bilateral blunt ocular trauma caused by an exercise resistance band (ERB) during a muscle-building exercise. Best-corrected decimal visual acuities (BCVAs) for right and left eyes were light perception and 0.15, respectively. The right eye was operated 10 days after injury for persistent vitreous hemorrhage, and the left eye 5 months later because of macular hole onset. After 36 months, the right eye showed extensive retinal degeneration (BCVA 0.04), and the left eye macular hole closure (BCVA 1.2). ERBs should be used cautiously as they can cause serious ocular trauma. en-copyright= kn-copyright= en-aut-name=AriyasuKanazu en-aut-sei=Ariyasu en-aut-mei=Kanazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KimuraShuhei en-aut-sei=Kimura en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatobaRyo en-aut-sei=Matoba en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Morizane HosokawaMio en-aut-sei=Morizane Hosokawa en-aut-mei=Mio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShiodeYusuke en-aut-sei=Shiode en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=DoiShinichiro en-aut-sei=Doi en-aut-mei=Shinichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KanzakiYuki en-aut-sei=Kanzaki en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MorizaneYuki en-aut-sei=Morizane en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=exercise resistance band kn-keyword=exercise resistance band en-keyword=traumatic macular hole kn-keyword=traumatic macular hole en-keyword=traumatic retinal degeneration kn-keyword=traumatic retinal degeneration en-keyword=bilateral blunt ocular trauma kn-keyword=bilateral blunt ocular trauma END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=3 article-no= start-page=291 end-page=295 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202206 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Factors Associated with Surgical Field Bacterial Detection during Total Hip Arthroplasty en-subtitle= kn-subtitle= en-abstract= kn-abstract=Total hip arthroplasty (THA) provides relief from hip pain and improves hip function. However, periprosthetic joint infection (PJI) remains an area of concern. We examined the detection rate of bacteria from surgical fields in wound closure, along with the relationship between bacterial detection rate and type of antiseptic, surgery time, and surgeon experience for 500 patients who underwent THA at our department. The mean age at surgery was 64.3 (± 27.3) years. The bacterial detection rate was 4.6%. None of the cases revealed PJI. No significant association between the detection rate and type of antiseptic used or surgery time was observed. However, for patients treated by surgeons with < 10 years of orthopedic experience, a detection rate of 7.3% was found, while a rate of 1.3% was observed for those treated by surgeons with ≥ 10 years of orthopedic experience. This finding indicated that orthopedic experience of less than 10 years was significantly associated with an increased bacterial detection rate (chi-square test, p=0.002). The detection rate was associated with surgeon experience but not with antiseptic type or surgery time. It is possible that intraoperative handling may increase the number of bacteria in surgical fields in wound closure. en-copyright= kn-copyright= en-aut-name=MiyakeYoshiaki en-aut-sei=Miyake en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MitaniShigeru en-aut-sei=Mitani en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NambaYoshifumi en-aut-sei=Namba en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UmeharaNorifumi en-aut-sei=Umehara en-aut-mei=Norifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawamotoToyohiro en-aut-sei=Kawamoto en-aut-mei=Toyohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=FuruichiShuro en-aut-sei=Furuichi en-aut-mei=Shuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= affil-num=2 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= affil-num=3 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School kn-affil= en-keyword=total hip arthroplasty kn-keyword=total hip arthroplasty en-keyword=bacterial contamination kn-keyword=bacterial contamination en-keyword=periprosthetic joint infection kn-keyword=periprosthetic joint infection END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=1 article-no= start-page=63 end-page=70 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Chidamide and Decitabine in Combination with a HAG Priming Regimen for Acute Myeloid Leukemia with TP53 Mutation en-subtitle= kn-subtitle= en-abstract= kn-abstract=We analyzed the treatment effects of chidamide and decitabine in combination with a HAG (homoharringtonine, cytarabine, G-CSF) priming regimen (CDHAG) in acute myeloid leukemia (AML) patients with TP53 mutation. Seven TP53 mutated AML patients were treated with CDHAG. The treatment effects were assessed using hemogram detection and bone marrow aspirate. The possible side effects were evaluated based on both hematological and non-hematological toxicity. Four of the seven patients were classified as having achieved complete remission after CDHAG treatment; one patient was considered to have achieved partial remission, and the remaining two patients were considered in non-remission. The overall response rate (ORR) to CDHAG was 71.4%. Regarding the side effects, the hematological toxicity level of the seven patients ranged from level III to level IV, and infections that occurred at lung, blood, and skin were recorded. Nausea, vomiting, liver injury, and kidney injury were also detected. However, all side effects were attenuated by proper management. The CDHAG regimen clearly improved the ORR (71.4%) of TP53-mutated AML patients, with no severe side effects. en-copyright= kn-copyright= en-aut-name=ZhangBei en-aut-sei=Zhang en-aut-mei=Bei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=PeiZhixin en-aut-sei=Pei en-aut-mei=Zhixin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WangHongxia en-aut-sei=Wang en-aut-mei=Hongxia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WuHuimin en-aut-sei=Wu en-aut-mei=Huimin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WangJunjie en-aut-sei=Wang en-aut-mei=Junjie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=BaiJunjun en-aut-sei=Bai en-aut-mei=Junjun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SongQinglin en-aut-sei=Song en-aut-mei=Qinglin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=2 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=3 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=4 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=5 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=6 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= affil-num=7 en-affil=Department of Hematology, Jiaozuo People’s Hospital kn-affil= en-keyword=acute myeloid leukemia kn-keyword=acute myeloid leukemia en-keyword=chidamide kn-keyword=chidamide en-keyword=decitabine kn-keyword=decitabine en-keyword=HAG kn-keyword=HAG en-keyword=TP53 mutation kn-keyword=TP53 mutation END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=1 article-no= start-page=51 end-page=56 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Obesity’s Influence on Insulin Resistance in Pregnant Women with Polycystic Ovary Syndrome en-subtitle= kn-subtitle= en-abstract= kn-abstract=Polycystic ovary syndrome (PCOS) is a common endocrine metabolic disorder that is associated with high insulin resistance and obesity. However, ~70% of women with PCOS in Japan are non-obese. We retrospectively analyzed the cases of 163 Japanese women with PCOS who visited our Ob/Gyn department in 2006-2018 to determine which has a greater effect on insulin resistance: PCOS or obesity. We reviewed the women’s medical records and calculated their insulin resistance and insulin secretion. The women’s mean age and pre-pregnancy body mass index (BMI) were 30±5.8 years and 24.8±5.6 kg/m2, respectively; their mean ± SD fasting plasma glucose, 94.1±13.7 mg/dL; HOMA-IR, 2.1±2.0; QUICKI, 0.4±0.0; and HOMA-β, 108.9±88.0%. Sixtyeight women were pregnant, and 37% (n=25) were obese (BMI ≥ 25 kg/m2). Obesity had a greater effect on insulin resistance: fasting plasma glucose F(1, 53)=6.134, p<0.05; fasting insulin F(1, 53)=31.606, p<0.01; HOMA-IR F(1, 53)=31.670, p<0.01; QUICKI F(1, 53)=16.156, p<0.01. There was no significant difference in values other than QUICKI and testosterone between the women with and without PCOS. Obesity thus had a greater effect on increased insulin resistance in pregnant women with PCOS. Further studies of the insulin resistance of non-obese women with PCOS is needed, as non-obese women with PCOS are common in Asia. en-copyright= kn-copyright= en-aut-name=EtoEriko en-aut-sei=Eto en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TaniKazumasa en-aut-sei=Tani en-aut-mei=Kazumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MakiJota en-aut-sei=Maki en-aut-mei=Jota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayataKei en-aut-sei=Hayata en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MasuyamaHisashi en-aut-sei=Masuyama en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=polycystic ovary syndrome kn-keyword=polycystic ovary syndrome en-keyword=insulin resistance kn-keyword=insulin resistance en-keyword=obesity kn-keyword=obesity en-keyword=pregnancy kn-keyword=pregnancy END start-ver=1.4 cd-journal=joma no-vol=76 cd-vols= no-issue=1 article-no= start-page=33 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=202202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Plasma Concentrations of Trace Elements Selenium and Cobalt During and After Coronary Artery Bypass Grafting Surgery en-subtitle= kn-subtitle= en-abstract= kn-abstract=Trace elements selenium (Se) and cobalt (Co) are essential in the human body, and a correlation between Se and cardiac surgery has been suggested. We investigated the plasma concentrations of Se and Co during and after coronary artery bypass grafting (CABG) surgery under cardiopulmonary bypass (CPB). From December 2019 to January 2020, preoperative plasma samples from isolated first-time CABG patients (n=20; 10 males, 10 females) were prospectively collected post-anesthesia and before CPB (T1), 45 min after CPB started (T2), 90 min after CPB started (T3), and postoperative days 1 (T4), and day 4 (T5). The plasma concentrations of Se and Co were measured. The Se concentration was significantly decreased at T2 (105.24±4.08 vs. 68.56±2.42 μg/L, p<0.001) and T3 (105.24±4.08 vs. 80.41±3.40 μg/L, p<0.001). The Co concentration was significantly decreased at T4 (0.35±0.19 vs. 0.26±0.13 μg/L, p<0.01) and T5 (0.35±0.19 vs. 0.23±0.11 μg/L, p<0.001). Five patients developed atrial fibrillation (AF); there was no other operative mortality or major morbidity. This is the first report of alterations of plasma Se and Co concentrations during and after CABG surgery. Our results may indicate that Se supplementation before or during CABG and Co supplementation after CABG may become necessary for patients undergoing CABG. en-copyright= kn-copyright= en-aut-name=ZhouJia-Yi en-aut-sei=Zhou en-aut-mei=Jia-Yi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HouHai-Tao en-aut-sei=Hou en-aut-mei=Hai-Tao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WangShi-Fu en-aut-sei=Wang en-aut-mei=Shi-Fu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YangQin en-aut-sei=Yang en-aut-mei=Qin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HeGuo-Wei en-aut-sei=He en-aut-mei=Guo-Wei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=The Institute of Cardiovascular Diseases & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital kn-affil= affil-num=2 en-affil=Unit of Perfusion, Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospita kn-affil= affil-num=3 en-affil=Unit of Perfusion, Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital kn-affil= affil-num=4 en-affil=The Institute of Cardiovascular Diseases & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital kn-affil= affil-num=5 en-affil=The Institute of Cardiovascular Diseases & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital kn-affil= en-keyword=trace element kn-keyword=trace element en-keyword=CABG kn-keyword=CABG en-keyword=cardiopulmonary bypass kn-keyword=cardiopulmonary bypass en-keyword=selenium kn-keyword=selenium en-keyword=cobalt kn-keyword=cobalt END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=6 article-no= start-page=751 end-page=754 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Novel Technique for Removing a Well-Fixed Cemented Cup with a Dedicated Original Device during Revision Total Hip Arthroplasty: Surgical Technique en-subtitle= kn-subtitle= en-abstract= kn-abstract=The improved cemented cup technique has attained excellent long-term results in primary total hip arthroplasty. When cup revision surgery was performed, the cemented cup, which was loosened at the bone-cement interface, was easily removed. However, with a well-fixed bone-cement interface, it remains difficult to remove the cemented cup for a revision in the event of a recurring dislocation. In addition, protrusions in the cement can cause unpredictable bone defects. A new removal device was created and used successfully to remove a well-fixed cemented cup safely and efficiently. This report introduces the device and the technique used in cemented cup removal. en-copyright= kn-copyright= en-aut-name=EndoHirosuke en-aut-sei=Endo en-aut-mei=Hirosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamadaKazuki en-aut-sei=Yamada en-aut-mei=Kazuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TestunagaTomonori en-aut-sei=Testunaga en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FurumatsuTakayuki en-aut-sei=Furumatsu en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MitaniShigeru en-aut-sei=Mitani en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School, Kurashiki City kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Denstry and Pharmaceutical Scineces kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Denstry and Pharmaceutical Scineces kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Denstry and Pharmaceutical Scineces kn-affil= affil-num=5 en-affil=Department of Bone and Joint Surgery, Kawasaki Medical School, Kurashiki City kn-affil= affil-num=6 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Denstry and Pharmaceutical Scineces kn-affil= en-keyword=total hip arthroplasty kn-keyword=total hip arthroplasty en-keyword=revision surgery kn-keyword=revision surgery en-keyword=well-fixed kn-keyword=well-fixed en-keyword=cemented cup kn-keyword=cemented cup END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=6 article-no= start-page=671 end-page=675 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Multiple Roles of Histidine-Rich Glycoprotein in Vascular Homeostasis and Angiogenesis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Histidine-rich glycoprotein (HRG) is a 75 kDa plasma protein that is synthesized in the liver of many verte-brates and present in their plasma at relatively high concentrations of 100-150 μg/mL. HRG is an abundant and well-characterized protein having a multidomain structure that enable it to interact with many ligands, func-tion as an adaptor molecule, and participate in numerous physiological and pathological processes. As a plasma protein, HRG has been reported to regulate vascular biology, including coagulation, fibrinolysis and angiogenesis, through its binding with several ligands (heparin, FXII, fibrinogen, thrombospondin, and plas-minogen) and interaction with many types of cells (endothelial cells, erythrocytes, neutrophils and platelets). This review aims to summarize the roles of HRG in maintaining vascular homeostasis and regulating angiogen-esis in various pathological conditions. en-copyright= kn-copyright= en-aut-name=GaoShangze en-aut-sei=Gao en-aut-mei=Shangze kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishiboriMasahiro en-aut-sei=Nishibori en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=histidine-rich glycoprotein kn-keyword=histidine-rich glycoprotein en-keyword=vascular biology kn-keyword=vascular biology en-keyword=coagulation kn-keyword=coagulation en-keyword=angiogenesis kn-keyword=angiogenesis END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=5 article-no= start-page=549 end-page=556 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Glial Cells as Possible Targets of Neuroprotection through Neurotrophic and Antioxidative Molecules in the Central and Enteric Nervous Systems in Parkinson’s Disease en-subtitle= kn-subtitle= en-abstract= kn-abstract=Parkinson’s disease (PD) is the second most common neurodegenerative disease worldwide. The loss of nigrostriatal dopaminergic neurons produces its characteristic motor symptoms, but PD patients also have non-motor symptoms such as constipation and orthostatic hypotension. The pathological hallmark of PD is the presence of α-synuclein-containing Lewy bodies and neurites in the brain. However, the PD pathology is observed in not only the central nervous system (CNS) but also in parts of the peripheral nervous system such as the enteric nervous system (ENS). Since constipation is a typical prodromal non-motor symptom in PD, often preceding motor symptoms by 10-20 years, it has been hypothesized that PD pathology propagates from the ENS to the CNS via the vagal nerve. Discovery of pharmacological and other methods to halt this progression of neurodegeneration in PD has the potential to improve millions of lives. Astrocytes protect neurons in the CNS by secretion of neurotrophic and antioxidative factors. Similarly, astrocyte-like enteric glial cells (EGCs) are known to secrete neuroprotective factors in the ENS. In this article, we summarize the neuroprotective function of astrocytes and EGCs and discuss therapeutic strategies for the prevention of neurodegeneration in PD targeting neurotrophic and antioxidative molecules in glial cells. en-copyright= kn-copyright= en-aut-name=IsookaNami en-aut-sei=Isooka en-aut-mei=Nami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyazakiIkuko en-aut-sei=Miyazaki en-aut-mei=Ikuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AsanumaMasato en-aut-sei=Asanuma en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Parkinson’s disease kn-keyword=Parkinson’s disease en-keyword=astrocyte kn-keyword=astrocyte en-keyword=enteric glial cell kn-keyword=enteric glial cell en-keyword=neurotrophic factor kn-keyword=neurotrophic factor en-keyword=antioxidative molecule kn-keyword=antioxidative molecule END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=4 article-no= start-page=543 end-page=548 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Usefulness of Middle Colic Artery Transposition Technique for Hepatic Arterial Reconstruction in Conversion Surgery for an Initially Unresectable, Locally Advanced Pancreatic Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=The outcomes of pancreatectomy with resection and reconstruction of the involved arteries for locally advanced pancreatic cancer following chemotherapy have improved in recent years. In pancreatic head cancers in which there is contact with the common and proper hepatic arteries, margin-negative resection requires pancreati-coduodenectomy, with the resection of these arteries and the restoration of hepatic arterial flow. Here, we describe a middle colic artery transposition technique in hepatic arterial reconstruction during pancreatoduo-denectomy for an initially unresectable locally advanced pancreatic cancer. This technique was effective and may provide a new option for hepatic artery reconstruction in such cases. en-copyright= kn-copyright= en-aut-name=YoshidaRyuichi en-aut-sei=Yoshida en-aut-mei=Ryuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YagiTakahito en-aut-sei=Yagi en-aut-mei=Takahito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YasuiKazuya en-aut-sei=Yasui en-aut-mei=Kazuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UmedaYuzo en-aut-sei=Umeda en-aut-mei=Yuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YoshidaKazuhiro en-aut-sei=Yoshida en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=FujiTomokazu en-aut-sei=Fuji en-aut-mei=Tomokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakagiKosei en-aut-sei=Takagi en-aut-mei=Kosei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KumanoKenjiro en-aut-sei=Kumano en-aut-mei=Kenjiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YoshimotoMasashi en-aut-sei=Yoshimoto en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=hepatic artery kn-keyword=hepatic artery en-keyword=locally advanced pancreatic cancer kn-keyword=locally advanced pancreatic cancer en-keyword=middle colic artery kn-keyword=middle colic artery en-keyword=pancreatoduodenectomy kn-keyword=pancreatoduodenectomy en-keyword=reconstruction technique kn-keyword=reconstruction technique END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=4 article-no= start-page=455 end-page=460 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Assessment of the Concordance Rate between Intraoperative Pathological Diagnosis and the Final Pathological Diagnosis of Spinal Cord Tumors en-subtitle= kn-subtitle= en-abstract= kn-abstract=The intraoperative pathological diagnosis (IPD) plays an important role in determining the optimal surgical treatment for spinal cord tumors. The final pathological diagnosis (FPD) is sometimes different from the IPD. Here, we sought to identify the accuracy of the IPD of spinal cord tumors compared to the FPD. We retrospec-tively analyzed the cases of 108 patients with spinal cord tumors treated surgically in our institute; the IPD, FPD, mismatched cases, and concordance rate between the IPD and FPD were investigated. Five cases involved a mismatch between the IPD and FPD. The overall concordance rate was 95.4%, with 90.9% for extra-dural lesions, 98.5% for intradural extramedullary lesions, 84.2% for intramedullary lesions, and 100% for dumbbell-type tumors. The concordance rate of intramedullary lesions tended to be lower than that of other lesions (p = 0.096). A lower concordance rate was revealed for intramedullary lesions compared to the other lesions. Despite the IPD clearly remaining a valuable tool during operative procedures, surgeons should recog-nize the limitations of IPDs and make comprehensive decisions about surgical treatments. en-copyright= kn-copyright= en-aut-name=MuraokaSosuke en-aut-sei=Muraoka en-aut-mei=Sosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamaneKentaro en-aut-sei=Yamane en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MisawaHaruo en-aut-sei=Misawa en-aut-mei=Haruo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakigawaTomoyuki en-aut-sei=Takigawa en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TetsunagaTomoko en-aut-sei=Tetsunaga en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OdaYoshiaki en-aut-sei=Oda en-aut-mei=Yoshiaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakanishiKazuo en-aut-sei=Nakanishi en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Intelligent Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=5 en-affil= kn-affil= affil-num=6 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Orthopaedic Surgery, Kawasaki Medical School Hospital kn-affil= affil-num=8 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=spinal cord tumor kn-keyword=spinal cord tumor en-keyword=intraoperative pathological diagnosis kn-keyword=intraoperative pathological diagnosis en-keyword= final pathological diagnosis kn-keyword= final pathological diagnosis en-keyword=concordance rate kn-keyword=concordance rate END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=4 article-no= start-page=403 end-page=413 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Surgical Treatment of Epiretinal Membrane en-subtitle= kn-subtitle= en-abstract= kn-abstract=Epiretinal membrane (ERM) is a common retinal disease characterized by cellular proliferation and metaplasia that lead to the formation of a pathological fibrocellular membrane immediately superjacent to the inner retinal surface. The vast majority of ERMs are considered idiopathic. However, ERM formation can result from various primary intraocular diseases, including retinal breaks and detachment, retinal vascular diseases, and vitreoretinal inflammatory conditions. Although ERMs are generally asymptomatic or cause mild metamorphopsia and/or a modest decrease in visual acuity, some can cause severe macular distortion and macular edema, resulting in significantly impaired function. Surgical removal of ERM is the only treatment, and improvements in vitrectomy systems have enabled less invasive treatment. However, there are currently no standardized criteria for ERM surgery, and the indications for surgery are determined from the patient’s subjective symptoms. Another problem with ERM surgery is that not all patients show satisfactory postoperative recovery of visual function. Thus, further research is needed to determine the criteria for ERM surgery and methods to improve the postoperative prognosis. en-copyright= kn-copyright= en-aut-name=MatobaRyo en-aut-sei=Matoba en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorizaneYuki en-aut-sei=Morizane en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=epiretinal membrane kn-keyword=epiretinal membrane en-keyword=vitrectomy kn-keyword=vitrectomy en-keyword=optical coherence tomography kn-keyword=optical coherence tomography en-keyword=internal limiting membrane kn-keyword=internal limiting membrane en-keyword=lamellar macular hole kn-keyword=lamellar macular hole END start-ver=1.4 cd-journal=joma no-vol=153 cd-vols= no-issue= article-no= start-page=98 end-page=108 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20218 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phase I dose-escalation study of endoscopic intratumoral injection of OBP-301 (Telomelysin) with radiotherapy in oesophageal cancer patients unfit for standard treatments en-subtitle= kn-subtitle= en-abstract= kn-abstract=Purpose: OBP-301 (Telomelysin) is an attenuated type-5 adenovirus that contains the human telomerase reverse transcriptase promoter to regulate viral replication. OBP-301 sensitises human cancer cells to ionising radiation by inhibiting DNA repair, and radiation enhances coxsackievirus and adenovirus receptor-mediated OBP-301 infection on the contrary. We assessed OBP-301 with radiotherapy in oesophageal cancer patients unfit for standard chemoradiation treatments.

Methods: A phase I dose-escalation study of OBP-301 with radiotherapy was conducted in 13 histologically confirmed oesophageal cancer patients deemed unfit to undergo surgery or chemotherapy. Study treatment consisted of OBP-301 administration by intratumoural needle injection using a flexible endoscope on days 1, 18 and 32. Radiotherapy was administered concurrently over 6 weeks, beginning on day 4, to a total of 60 Gy.

Results: Of the 13 patients, 7, 3 and 3 patients were treated with 10(10), 10(11) and 10(12) virus particles, respectively. Study group comprised 10 males and 3 females, with a median age of 82 years (range, 53-91 years). All patients developed a transient, self-limited lymphopenia. Distribution studies revealed transient virus shedding in the plasma. Eight patients had local complete response (CR); all of them exhibited no pathologically viable malignant cells in biopsy specimens, and 3 patients had a partial response. The objective response rate was 91.7%. The clinical CR rate was 83.3% in stage I and 60.0% in stage II/III. Histopathological examination revealed massive infiltration of CD8 thorn cells and increased PD-L1 expression.

Conclusion: Multiple courses of endoscopic intratumoural OBP-301 injection with radiotherapy are feasible and provide clinical benefits in patients with oesophageal cancer unfit for standard treatments. (C) 2021 Elsevier Ltd. All rights reserved. en-copyright= kn-copyright= en-aut-name=ShirakawaYasuhiro en-aut-sei=Shirakawa en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TazawaHiroshi en-aut-sei=Tazawa en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TanabeShunsuke en-aut-sei=Tanabe en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KanayaNobuhiko en-aut-sei=Kanaya en-aut-mei=Nobuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NomaKazuhiro en-aut-sei=Noma en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KoujimaTakeshi en-aut-sei=Koujima en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KashimaHajime en-aut-sei=Kashima en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KatoTakuya en-aut-sei=Kato en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KurodaShinji en-aut-sei=Kuroda en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KikuchiSatoru en-aut-sei=Kikuchi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KatsuiKuniaki en-aut-sei=Katsui en-aut-mei=Kuniaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=KanazawaSusumu en-aut-sei=Kanazawa en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=UrataYasuo en-aut-sei=Urata en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=11 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=12 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=13 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=14 en-affil=Oncolys BioPharma, Inc. kn-affil= affil-num=15 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Telomerase kn-keyword=Telomerase en-keyword=adenovirus kn-keyword=adenovirus en-keyword=radiotherapy kn-keyword=radiotherapy en-keyword=esophageal cancer kn-keyword=esophageal cancer en-keyword=immunotherapy kn-keyword=immunotherapy END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=3 article-no= start-page=381 end-page=384 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Presence of Microplastics in Four Types of Shellfish Purchased at Fish Markets in Okayama City, Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=The worldwide microplastic pollution in our environment is a matter of great concern. Harmful effects of plastics have been reported in various types of organisms including murine animals. We examined the presence of microplastics in four types of shellfish purchased from fish markets in Okayama, Japan and served to the public: short-neck clam (Ruditapes philippinarum, asari in Japanese), hard-shell clam (Meretrix lusoria, hamaguri), brackishwater clam (Cyrenidae, shijimi), and oyster (Crassostrea gigas, kaki). Our analyses demonstrated that approx. 3 pieces of microplastics were present per single shellfish, based on the division of the total number of pieces of microplastic obtained from all 4 types of shellfish by the total number of shellfish examined. Since health problems in humans due to microplastics have not yet been confirmed, further examinations of the effects of ingested microplastics are needed. en-copyright= kn-copyright= en-aut-name=YamamotoKen-ichi en-aut-sei=Yamamoto en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OshikiToshiyuki en-aut-sei=Oshiki en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KagawaHiroko en-aut-sei=Kagawa en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NambaMasayoshi en-aut-sei=Namba en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SakaguchiMasakiyo en-aut-sei=Sakaguchi en-aut-mei=Masakiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=3 en-affil=Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=microplastics, kn-keyword=microplastics, en-keyword=shellfish kn-keyword=shellfish en-keyword=Japan kn-keyword=Japan en-keyword= health effect kn-keyword= health effect en-keyword=pollution kn-keyword=pollution END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=3 article-no= start-page=373 end-page=379 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202106 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationships among Bone Metabolic Markers, Body Fat Composition and Carotid Intima–Media Thickness in Premenopausal Obese Women en-subtitle= kn-subtitle= en-abstract= kn-abstract=Osteocalcin (OC) is inversely related to body fat distribution and fasting glucose levels. We sought to observe the effect of OC on fat distribution and subclinical atherosclerosis as measured by carotid intima-media thickness (CIMT) in premenopausal obese women. In this prospective observational study, totally, 73 premenopausal obese women (aged 17-55 years) and 53 healthy women (aged 20-50 years) with normal weight were included as controls. Anthropometric measurements, total fat and fat ratio, insulin, fasting blood glucose, and OC levels were estimated. Ultrasonography was used to assess fat distribution, and fat thickness was measured in 4 regions. Subcutaneous fat (SCF), visceral fat (VF), and preperitoneal fat (PPF) thicknesses were considerably higher in obese subjects (p<0.01) than healthy controls, while OC levels were significantly lower. No correlation was observed between OC levels and SCF, VF, or PPF. In a multiple regression analysis, OC was significantly positively associated with SCF (p=0.04, Beta=0.284). No associations were observed between OC levels and VF, PPF, or CIMT. A significant association was observed between parathyroid hormone (PTH) and VF (p=0.021, Beta=0.284), and vitamin D levels were inversely associated with VF (p=0.002, r=−0.366). OC levels were lower in premenopausal obese women than normal-weight healthy controls, but OC exhibited no correlation with VF or PPF, and only a weak positive association with SCF. Additionally, VF was positively correlated with PTH and inversely correlated with vitamin D. These results suggest that OC may be an early indicator of lipid accumulation in te subcutaneous area and development of atherosclerosis. en-copyright= kn-copyright= en-aut-name=YaylaliGuzin F. en-aut-sei=Yaylali en-aut-mei=Guzin F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DedeogluOzen en-aut-sei=Dedeoglu en-aut-mei=Ozen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TopsakalSenay en-aut-sei=Topsakal en-aut-mei=Senay kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HerekDuygu en-aut-sei=Herek en-aut-mei=Duygu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SenolHande en-aut-sei=Senol en-aut-mei=Hande kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Endocrinology and Metabolism, Pamukkale University kn-affil= affil-num=2 en-affil=Department ofInternal Medicine, Pamukkale University kn-affil= affil-num=3 en-affil=Department of Endocrinology and Metabolism, Pamukkale University kn-affil= affil-num=4 en-affil=Department of Radiology, Pamukkale University kn-affil= affil-num=5 en-affil=Department of Biostatistics, Pamukkale University kn-affil= en-keyword=body fat composition kn-keyword=body fat composition en-keyword=carotid intima-media thickness kn-keyword=carotid intima-media thickness en-keyword=obesity kn-keyword=obesity en-keyword=osteocalcin kn-keyword=osteocalcin en-keyword=premenopausal women kn-keyword=premenopausal women END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=2 article-no= start-page=243 end-page=248 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pyogenic Ventriculitis After Anterior Skull Base Surgery Treated With Endoscopic Ventricular Irrigation And Reconstruction Using a Vascularized Flap en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ventriculitis is a rare, serious complication of neurosurgery. A 59-year-old man who had undergone a craniotomy for a paranasal adenocarcinoma, developed a right frontal cystic lesion. We performed a bifrontal craniotomy to remove the lesion. The dura was repaired with non-vascularized free fascia lata in watertight fashion. Ventriculitis occurred 3 days postoperatively. Ventricular drainage, craniectomy, and endoscopic irrigation were undertaken to remove an abscess. The dura and the resection cavity were reconstructed using a vascularized anterolateral thigh adipofascial flap. His symptoms disappeared, indicating that endoscopic irrigation and reconstruction can effectively address ventriculitis even in patients in critical clinical condition. en-copyright= kn-copyright= en-aut-name=TomitaYusuke en-aut-sei=Tomita en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimazuYosuke en-aut-sei=Shimazu en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawakamiMasato en-aut-sei=Kawakami en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsumotoHiroshi en-aut-sei=Matsumoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiiKentaro en-aut-sei=Fujii en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KamedaMasahiro en-aut-sei=Kameda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YasuharaTakao en-aut-sei=Yasuhara en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SurugaYasuki en-aut-sei=Suruga en-aut-mei=Yasuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OtaTomoyuki en-aut-sei=Ota en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KimataYoshihiro en-aut-sei=Kimata en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KurozumiKazuhiko en-aut-sei=Kurozumi en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=DateIsao en-aut-sei=Date en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= affil-num=1 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=11 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=12 en-affil=Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=ventriculitis kn-keyword=ventriculitis en-keyword=surgical site infection kn-keyword=surgical site infection en-keyword=intraventricular antimicrobial therapy kn-keyword=intraventricular antimicrobial therapy en-keyword=anterior skull base surgery kn-keyword=anterior skull base surgery END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=2 article-no= start-page=231 end-page=238 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Long-term Survival with a Rare Advanced Primary Gastrointestinal Malignant Melanoma Treated with Laparoscopic Surgery/Immune Checkpoint Inhibitor en-subtitle= kn-subtitle= en-abstract= kn-abstract=Targeted therapies for malignant melanoma have improved patients’ prognoses. A primary gastrointestinal malignant melanoma is very rare, with no standard treatment strategy. We treated a 78-year-old Japanese female with advanced primary gastrointestinal melanoma of the descending colon and gallbladder. We administered a multidisciplinary treatment: surgical resection of the descending colon and gallbladder tumors, resection of the metastatic lymph nodes behind the pancreas head, and immune checkpoint antibody-blockade therapy (nivolumab) for ~4 years. PET/CT demonstrated no recurrent lesion for > 3 years. Multidisciplinary therapies (e.g., surgery, chemotherapy, radiotherapy, target therapy, and immune checkpoint antibody-blockade therapy) can successfully treat primary gastrointestinal malignant melanoma. en-copyright= kn-copyright= en-aut-name=EndoMotochika en-aut-sei=Endo en-aut-mei=Motochika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YanoShuya en-aut-sei=Yano en-aut-mei=Shuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AsanoHiroaki en-aut-sei=Asano en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakedaSho en-aut-sei=Takeda en-aut-mei=Sho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HamadaYuki en-aut-sei=Hamada en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KondoYoshitaka en-aut-sei=Kondo en-aut-mei=Yoshitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KurodaShinji en-aut-sei=Kuroda en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ShigeyasuKunitoshi en-aut-sei=Shigeyasu en-aut-mei=Kunitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KikuchiSatoru en-aut-sei=Kikuchi en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TeraishiFuminori en-aut-sei=Teraishi en-aut-mei=Fuminori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=NishizakiMasahiko en-aut-sei=Nishizaki en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Surgery, Mitoyo general Hospital kn-affil= affil-num=4 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Pathology, Okayama University Hospital kn-affil= affil-num=11 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=12 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=13 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=14 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=primary gastrointestinal melanoma kn-keyword=primary gastrointestinal melanoma en-keyword=laparoscopic surgery kn-keyword=laparoscopic surgery en-keyword=immune checkpoint antibody-blockade inhibitor kn-keyword=immune checkpoint antibody-blockade inhibitor END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=2 article-no= start-page=199 end-page=204 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Laparoscopic Hepatectomy for the Patient with Hemophilia A with High Titer Factor VIII Inhibitor en-subtitle= kn-subtitle= en-abstract= kn-abstract=We present the first case of laparoscopic left lateral segmentectomy for hepatocellular carcinoma (HCC) in a patient with hemophilia A, acquired hepatitis C, and high-titer factor VIII inhibitor, which was confirmed by preoperative diagnosis. He underwent laparoscopic left lateral segmentectomy with the administration of recombinant activated factor VII. Surgery could be performed with reduced intraoperative hemorrhage. He experienced postoperative intra-abdominal wall hemorrhage, which was successfully managed with red cell concentrates transfusion and administration of recombinant activated factor VII. Laparoscopic hepatectomy can be applied for hemophilia patients with high titer inhibitors. en-copyright= kn-copyright= en-aut-name=MatsudaTatsuo en-aut-sei=Matsuda en-aut-mei=Tatsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UmedaYuzo en-aut-sei=Umeda en-aut-mei=Yuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshidaKazuhiro en-aut-sei=Yoshida en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsudaTadakazu en-aut-sei=Matsuda en-aut-mei=Tadakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=UnoMasatoshi en-aut-sei=Uno en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AbeMasaya en-aut-sei=Abe en-aut-mei=Masaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AsadaNoboru en-aut-sei=Asada en-aut-mei=Noboru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MaedaYoshinobu en-aut-sei=Maeda en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YagiTakahito en-aut-sei=Yagi en-aut-mei=Takahito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Surgery, Matsuda Hospital kn-affil= affil-num=5 en-affil=Department of Internal Medicine, Kaneda Hospital kn-affil= affil-num=6 en-affil=Department of Hematology and Oncology,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Hematology and Oncology,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Hematology and Oncology,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterological Surgery,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=hemophilia A kn-keyword=hemophilia A en-keyword=hepatectomy kn-keyword=hepatectomy en-keyword=inhibitor kn-keyword=inhibitor en-keyword=laparoscopy kn-keyword=laparoscopy en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=2 article-no= start-page=153 end-page=167 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lactoferrin-like Immunoreactivity in Distinct Neuronal Populations in the Mouse Central Nervous System en-subtitle= kn-subtitle= en-abstract= kn-abstract=Lactoferrin (Lf) is an iron-binding glycoprotein mainly found in exocrine secretions and the secondary granules of neutrophils. In the central nervous system (CNS), expression of the Lf protein has been reported in the lesions of some neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis, as well as in the aged brain. Lf is primarily considered an iron chelator, protecting cells from potentially toxic iron or iron-requiring microorganisms. Other biological functions of Lf include immunomodulation and transcriptional regulation. However, the roles of Lf in the CNS have yet to be fully clarified. In this study, we raised an antiserum against mouse Lf and investigated the immunohistochemical localization of Lf-like immunoreactivity (Lf-LI) throughout the CNS of adult mice. Lf-LI was found in some neuronal populations throughout the CNS. Intense labeling was found in neurons in the olfactory systems, hypothalamic nuclei, entorhinal cortex, and a variety of brainstem nuclei. This study provides detailed information on the Lf-LI distribution in the CNS, and the findings should promote further understanding of both the physiological and pathological significance of Lf in the CNS. en-copyright= kn-copyright= en-aut-name=ShimaokaShigeyoshi en-aut-sei=Shimaoka en-aut-mei=Shigeyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HamaokaHitomi en-aut-sei=Hamaoka en-aut-mei=Hitomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueJunji en-aut-sei=Inoue en-aut-mei=Junji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AsanumaMasato en-aut-sei=Asanuma en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TooyamaIkuo en-aut-sei=Tooyama en-aut-mei=Ikuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KondoYoichi en-aut-sei=Kondo en-aut-mei=Yoichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University kn-affil= affil-num=2 en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University kn-affil= affil-num=3 en-affil=Department of Anatomy and Cell Biology, Division of Life Sciences, Osaka Medical and Pharmaceutical University kn-affil= affil-num=4 en-affil=Department of Medical Neurobiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science kn-affil= affil-num=6 en-affil=Molecular Neuroscience Research Center, Shiga University of Medical Science kn-affil= en-keyword=lactoferrin kn-keyword=lactoferrin en-keyword=immunohistochemistry kn-keyword=immunohistochemistry en-keyword=brain mapping kn-keyword=brain mapping END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=2 article-no= start-page=139 end-page=145 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of the Imaging Process for a Novel Subtraction Method Using Apparent Diffusion Coefficient Values en-subtitle= kn-subtitle= en-abstract= kn-abstract=Diffusion-weighted imaging may be used to obtain the apparent diffusion coefficient (ADC), which aids the diagnosis of cerebral infarction and tumors. An ADC reflects elements of free diffusion. Diffusion kurtosis imaging (DKI) has attracted attention as a restricted diffusion imaging technique. The ADC subtraction method (ASM) was developed to visualize restricted diffusion with high resolution by using two ADC maps taken with different diffusion times. We conducted the present study to provide a bridge between the reported basic ASM research and clinical research. We developed new imaging software for clinical use and evaluated its performance herein. This software performs the imaging process automatically and continuously at the pixel level, using ImageJ software. The new software uses a macro or a plugin which is compatible with various operating systems via a Java Virtual Machine. We tested the new imaging software’s performance by using a Jurkat cell bio-phantom, and the statistical evaluation of the performance clarified that the ASM values of 99.98% of the pixels in the bio-phantom and physiological saline were calculated accurately (p<0.001). The new software may serve as a useful tool for future clinical applications and restricted diffusion imaging research. en-copyright= kn-copyright= en-aut-name=HamadaKentaro en-aut-sei=Hamada en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KurodaMasahiro en-aut-sei=Kuroda en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YoshimuraYuuki en-aut-sei=Yoshimura en-aut-mei=Yuuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KhasawnehAbdullah en-aut-sei=Khasawneh en-aut-mei=Abdullah kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=BarhamMajd en-aut-sei=Barham en-aut-mei=Majd kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TekikiNouha en-aut-sei=Tekiki en-aut-mei=Nouha kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SugiantoIrfan en-aut-sei=Sugianto en-aut-mei=Irfan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=BamgboseBabatunde O. en-aut-sei=Bamgbose en-aut-mei=Babatunde O. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KonishiKohei en-aut-sei=Konishi en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SugimotoKohei en-aut-sei=Sugimoto en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IshizakaHinata en-aut-sei=Ishizaka en-aut-mei=Hinata kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KurozumiAkira en-aut-sei=Kurozumi en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=MatsushitaToshi en-aut-sei=Matsushita en-aut-mei=Toshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=OhnoSeiichiro en-aut-sei=Ohno en-aut-mei=Seiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=KanazawaSusumu en-aut-sei=Kanazawa en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=AsaumiJunichi en-aut-sei=Asaumi en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= affil-num=1 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=2 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=3 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=4 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= affil-num=9 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=10 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=11 en-affil=Radiological Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=12 en-affil=Central Division of Radiology, Okayama University Hospital kn-affil= affil-num=13 en-affil=Central Division of Radiology, Okayama University Hospital kn-affil= affil-num=14 en-affil=Central Division of Radiology, Okayama University Hospital kn-affil= affil-num=15 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=16 en-affil=Department of Oral and Maxillofacial Radiology, Okayama University Hospital kn-affil= en-keyword=apparent diffusion coefficient kn-keyword=apparent diffusion coefficient en-keyword=ADC subtraction method kn-keyword=ADC subtraction method en-keyword=restricted diffusion kn-keyword=restricted diffusion en-keyword=ImageJ kn-keyword=ImageJ en-keyword=plugin kn-keyword=plugin END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue=1 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210304 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Exploratory classification of clinical phenotypes in Japanese patients with antineutrophil cytoplasmic antibody-associated vasculitis using cluster analysis en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel patient cluster in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) may be identified in Japan. We performed multiple correspondence and cluster analysis regarding 427 clinically diagnosed AAV patients excluding eosinophilic granulomatosis with polyangiitis. Model 1 included the ANCA phenotype, items of the Birmingham Vasculitis Activity Score, and interstitial lung disease; model 2 included serum creatinine (s-Cr) and C-reactive protein (CRP) levels with model 1 components. In seven clusters determined in model 1, the ANCA-negative (n=8) and proteinase 3-ANCA-positive (n=41) groups emerged as two distinct clusters. The other five myeloperoxidase-ANCA-positive clusters were characterized by ear, nose, and throat (ENT) (n=47); cutaneous (n=36); renal (n=256), non-renal (n=33); and both ENT and cutaneous symptoms (n=6). Four clusters in model 2 were characterized by myeloperoxidase-ANCA negativity (n=42), without s-Cr elevation (<1.3 mg/dL) (n=157), s-Cr elevation (1.3 mg/dL) with high CRP (>10 mg/dL) (n=71), or s-Cr elevation (>= 1.3 mg/dL) without high CRP (<= 10 mg/dL) (n=157). Overall, renal, and relapse-free survival rates were significantly different across the four clusters in model 2. ENT, cutaneous, and renal symptoms may be useful in characterization of Japanese AAV patients with myeloperoxidase-ANCA. The combination of s-Cr and CRP levels may be predictive of prognosis. en-copyright= kn-copyright= en-aut-name=WatanabeHaruki en-aut-sei=Watanabe en-aut-mei=Haruki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SadaKen-ei en-aut-sei=Sada en-aut-mei=Ken-ei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HarigaiMasayoshi en-aut-sei=Harigai en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AmanoKoichi en-aut-sei=Amano en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=DobashiHiroaki en-aut-sei=Dobashi en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakasakiYoshinari en-aut-sei=Takasaki en-aut-mei=Yoshinari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FujimotoShouichi en-aut-sei=Fujimoto en-aut-mei=Shouichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AtsumiTatsuya en-aut-sei=Atsumi en-aut-mei=Tatsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamagataKunihiro en-aut-sei=Yamagata en-aut-mei=Kunihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HommaSakae en-aut-sei=Homma en-aut-mei=Sakae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=ArimuraYoshihiro en-aut-sei=Arimura en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=Research Committee of Intractable Vasculitis Syndrome (JPVAS) & Research Committee of Intractable Renal Disease of the Ministry of Health, Labour, and Welfare of Japan en-aut-sei=Research Committee of Intractable Vasculitis Syndrome (JPVAS) & Research Committee of Intractable Renal Disease of the Ministry of Health, Labour, and Welfare of Japan en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Department of Rheumatology, Tokyo Women’s Medical University School of Medicine kn-affil= affil-num=4 en-affil=Department of Rheumatology and Clinical Immunology, Saitama Medical Center, Saitama Medical University kn-affil= affil-num=5 en-affil=Division of Hematology, Rheumatology and Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Kagawa University kn-affil= affil-num=6 en-affil=Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine kn-affil= affil-num=7 en-affil=Department of Hemovascular Medicine and Artificial Organs, Faculty of Medicine, University of Miyazaki kn-affil= affil-num=8 en-affil=Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University kn-affil= affil-num=9 en-affil=Department of Nephrology, Faculty of Medicine, University of Tsukuba kn-affil= affil-num=10 en-affil=Department of Advanced and Integrated Interstitial Lung Diseases Research, School of Medicine, Toho University kn-affil= affil-num=11 en-affil=Department of Nephrology and Rheumatology, Kyorin University School of Medicine kn-affil= affil-num=12 en-affil=Okayama University kn-affil= affil-num=13 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=413 cd-vols= no-issue= article-no= start-page=3339 end-page=3347 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210313 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Speciation of chromium in water samples using microfluidic paper-based analytical devices with online oxidation of trivalent chromium en-subtitle= kn-subtitle= en-abstract= kn-abstract= Speciation of chromium (Cr) was demonstrated using microfluidic paper-based analytical devices (μ-PADs) that permit the colorimetric determination of hexavalent chromium (Cr(VI)) and trivalent chromium (Cr(III)) via online oxidation. The μ-PADs consist of left and right channels that allow the simultaneous measurements of Cr(VI) and total Cr based on the colorimetric reaction of Cr(VI) with 1,5-diphenylcarbazide (DPC). For the determination of Cr(VI), a sample solution was directly reacted with DPC in the left channels whereas total Cr was determined in the right channels, which permitted online oxidation in the pretreatment zone containing cerium (IV) (Ce(IV)) followed by a colorimetric reaction with DPC. We found that the online oxidation of Cr(III) proceeded 100% whereas Ce(IV) inhibited the reaction of Cr(VI) with DPC. Therefore, speciation can be achieved by measuring the Cr(VI) and total Cr in the left and right channels followed by the subtraction of Cr(VI) from total Cr. The limits of detection and quantification were 0.008 and 0.02 mg L−1 for Cr(VI) and 0.07 and 0.1 mg L−1 for Cr(III) or total Cr, respectively. The linear dynamic ranges were 0.02–100 mg L−1 and 0.1–60 mg L−1 for Cr(VI) and Cr(III), respectively. The RSDs were less than 7.5%. The results obtained using μ-PADs were in good agreement with those obtained via ICP-OES with recoveries of 92–108% for Cr(III) and 108–110% for Cr (VI) using μ-PADs, and 106–110% for total Cr using ICP-OES. Thus, the μ-PADs could potentially be utilized for the speciation of chromium in developing countries where environmental pollution and the availability of sophisticated instruments are significant problems. en-copyright= kn-copyright= en-aut-name=MuhammedAbdellah en-aut-sei=Muhammed en-aut-mei=Abdellah kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HussenAhmed en-aut-sei=Hussen en-aut-mei=Ahmed kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Center for Environmental Science, College of Natural and Computational Sciences, Addis Ababa University kn-affil= affil-num=2 en-affil=Center for Environmental Science, College of Natural and Computational Sciences, Addis Ababa University kn-affil= affil-num=3 en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil= en-keyword=Microfluidic paper-based analytical device kn-keyword=Microfluidic paper-based analytical device en-keyword=Chromium kn-keyword=Chromium en-keyword=Cr(III) kn-keyword=Cr(III) en-keyword=Cr(VI) kn-keyword=Cr(VI) en-keyword=Online oxidation kn-keyword=Online oxidation en-keyword=Speciation kn-keyword=Speciation END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=1 article-no= start-page=1100 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210217 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structure of photosystem I-LHCI-LHCII from the green alga Chlamydomonas reinhardtii in State 2 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Photosystem I (PSI) and II (PSII) balance their light energy distribution absorbed by their light-harvesting complexes (LHCs) through state transition to maintain the maximum photosynthetic performance and to avoid photodamage. In state 2, a part of LHCII moves to PSI, forming a PSI-LHCI-LHCII supercomplex. The green alga Chlamydomonas reinhardtii exhibits state transition to a far larger extent than higher plants. Here we report the cryo-electron microscopy structure of a PSI-LHCI-LHCII supercomplex in state 2 from C. reinhardtii at 3.42 Å resolution. The result reveals that the PSI-LHCI-LHCII of C. reinhardtii binds two LHCII trimers in addition to ten LHCI subunits. The PSI core subunits PsaO and PsaH, which were missed or not well-resolved in previous Cr-PSI-LHCI structures, are observed. The present results reveal the organization and assembly of PSI core subunits, LHCI and LHCII, pigment arrangement, and possible pathways of energy transfer from peripheral antennae to the PSI core. en-copyright= kn-copyright= en-aut-name=HuangZihui en-aut-sei=Huang en-aut-mei=Zihui kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShenLiangliang en-aut-sei=Shen en-aut-mei=Liangliang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WangWenda en-aut-sei=Wang en-aut-mei=Wenda kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MaoZhiyuan en-aut-sei=Mao en-aut-mei=Zhiyuan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YiXiaohan en-aut-sei=Yi en-aut-mei=Xiaohan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KuangTingyun en-aut-sei=Kuang en-aut-mei=Tingyun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShenJian-Ren en-aut-sei=Shen en-aut-mei=Jian-Ren kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ZhangXing en-aut-sei=Zhang en-aut-mei=Xing kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HanGuangye en-aut-sei=Han en-aut-mei=Guangye kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine kn-affil= affil-num=2 en-affil=Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences kn-affil= affil-num=3 en-affil=Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences kn-affil= affil-num=4 en-affil=Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences kn-affil= affil-num=5 en-affil=Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine kn-affil= affil-num=6 en-affil=Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences kn-affil= affil-num=7 en-affil=Institute for Interdisciplinary Science, and Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=8 en-affil=Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine kn-affil= affil-num=9 en-affil=Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences kn-affil= END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=1 article-no= start-page=87 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=202102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Long-term Follow-up of Living-Donor Kidney Transplantation after Cadaveric Lung Transplantation en-subtitle= kn-subtitle= en-abstract= kn-abstract=Although chronic kidney disease (CKD) commonly develops after lung transplantation (LT), living-donor kid-ney transplantation (LDKT) for CKD after LT is known to provide favorable outcomes. We describe the long-term follow-up findings of a patient who underwent LDKT after bilateral cadaveric LT. A 37-year-old male underwent LDKT for CKD 18 years after receiving bilateral cadaveric LT. He developed chronic lung allograft dysfunction (CLAD) 20 years after the LT; however, at 26 years after the initial LT, he is still alive with no pro-gression of CLAD or CKD. KT could be a viable option for CKD even after LT in Japan. en-copyright= kn-copyright= en-aut-name=ShiotaniToshio en-aut-sei=Shiotani en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SugimotoSeiichiro en-aut-sei=Sugimoto en-aut-mei=Seiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiKota en-aut-sei=Araki en-aut-mei=Kota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TomiokaYasuaki en-aut-sei=Tomioka en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyoshiKentaroh en-aut-sei=Miyoshi en-aut-mei=Kentaroh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OtaniShinji en-aut-sei=Otani en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamaneMasaomi en-aut-sei=Yamane en-aut-mei=Masaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ToyookaShinichi en-aut-sei=Toyooka en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Organ Transplant Center, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Organ Transplant Center, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of General Thoracic Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Organ Transplant Center, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of General Thoracic Surgery, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of General Thoracic Surgery, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of General Thoracic Surgery, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of General Thoracic Surgery, Okayama University Hospital kn-affil= en-keyword=lung transplantation kn-keyword=lung transplantation en-keyword=kidney transplantation kn-keyword=kidney transplantation en-keyword=chronic kidney disease kn-keyword=chronic kidney disease en-keyword=non-tuberculous mycobacterial infection kn-keyword=non-tuberculous mycobacterial infection en-keyword=chronic lung allograft dysfunction kn-keyword=chronic lung allograft dysfunction END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=6 article-no= start-page=531 end-page=535 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Soft Tissue Myoepithelioma of the Shoulder en-subtitle= kn-subtitle= en-abstract= kn-abstract=Soft tissue myoepitheliomas are often misdiagnosed due to their rarity. Herein, we describe a case of soft tissue myoepithelioma of the shoulder. A 72-year-old woman had a suspected sarcoma on her shoulder and under-went open biopsy. She was referred to our hospital, where the tumor was widely resected and the diagnosis of myoepithelioma was histologically confirmed. No recurrence has been observed in the 3 years since the sur-gery. Careful and prompt planning is necessary for the effective treatment of myoepithelioma. en-copyright= kn-copyright= en-aut-name=HashimotoKazuhiko en-aut-sei=Hashimoto en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishimuraShunji en-aut-sei=Nishimura en-aut-mei=Shunji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ChikugoTakaaki en-aut-sei=Chikugo en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KakinokiRyosuke en-aut-sei=Kakinoki en-aut-mei=Ryosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AkagiMasao en-aut-sei=Akagi en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Orthopedic Surgery, Kindai University Hospital kn-affil= affil-num=2 en-affil=Department of Orthopedic Surgery, Kindai University Hospital kn-affil= affil-num=3 en-affil=Department of Pathology, Kindai University Hospital kn-affil= affil-num=4 en-affil=Department of Orthopedic Surgery, Kindai University Hospital kn-affil= affil-num=5 en-affil=Department of Orthopedic Surgery, Kindai University Hospital kn-affil= en-keyword=soft tissue myoepithelioma kn-keyword=soft tissue myoepithelioma en-keyword=unplanned resection kn-keyword=unplanned resection en-keyword=shoulder kn-keyword=shoulder END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=6 article-no= start-page=521 end-page=524 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202012 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bilateral Approach for Thoracoscopic Esophagectomy in a Patient with Esophageal Cancer and Solitary Posterior Thoracic Para-aortic Lymph Node Metastasis en-subtitle= kn-subtitle= en-abstract= kn-abstract=We report a successful dissection of metastatic posterior thoracic para-aortic lymph node (No. 112aoP) via bilateral thoracoscopic surgery. With the anesthetized patient (a 73-year-old Japanese woman) in the prone position, two working ports were inserted for the left-side approach, and artificial pneumothorax was created. Thoracoscopic examination revealed a swollen LN posterior to the descending aorta. Fat and metastatic LNs posterior to the aorta were dissected from the aortic arch level to the diaphragm while preserving intercostal arteries. For the right-side approach, two working ports were inserted and a routine thoracoscopic esophagec-tomy was performed. Gastric conduit reconstruction was achieved laparoscopically. Operation time for the left thoracic procedure: 54 min; estimated blood loss: almost none. No recurrence was detected 24 months post-operatively. There are several surgical options for approaching No. 112aoP, including transhiatal, left thora-cotomy, and thoracoscopy. Although a wide dissection of the posterior thoracic para-aortic area has not been reported, it may be feasible and safe if the artery of Adamkiewicz and intercostal arteries are preserved. A min-imally invasive bilateral thoracoscopic approach for a thoracoscopic esophagectomy is safe and useful for esophageal cancer patients with solitary No. 112aoP metastasis. en-copyright= kn-copyright= en-aut-name=ItazakiYujiro en-aut-sei=Itazaki en-aut-mei=Yujiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujimotoHironori en-aut-sei=Tsujimoto en-aut-mei=Hironori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SugasawaHidekazu en-aut-sei=Sugasawa en-aut-mei=Hidekazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YaguchiYoshihisa en-aut-sei=Yaguchi en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NomuraShinsuke en-aut-sei=Nomura en-aut-mei=Shinsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ItoNozomi en-aut-sei=Ito en-aut-mei=Nozomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HaradaManabu en-aut-sei=Harada en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SugiharaTakao en-aut-sei=Sugihara en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TsuchiyaSatoshi en-aut-sei=Tsuchiya en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IshibashiYusuke en-aut-sei=Ishibashi en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KouzuKeita en-aut-sei=Kouzu en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KishiYoji en-aut-sei=Kishi en-aut-mei=Yoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=UenoHideki en-aut-sei=Ueno en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=2 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=3 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=4 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=5 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=6 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=7 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=8 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=9 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=10 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=11 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=12 en-affil=Department of Surgery, National Defense Medical College kn-affil= affil-num=13 en-affil=Department of Surgery, National Defense Medical College kn-affil= en-keyword=bilateral approach kn-keyword=bilateral approach en-keyword=posterior thoracic para-aortic lymph node kn-keyword=posterior thoracic para-aortic lymph node en-keyword=thoracoscopic esophagectomy kn-keyword=thoracoscopic esophagectomy END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=5 article-no= start-page=401 end-page=406 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of Prognosis of Juvenile Differentiated Thyroid Carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract=Differentiated thyroid carcinoma (DTC) in juvenile patients is often an extensive and aggressive disease with a high frequency of recurrence. However, the prognosis is excellent, with a low mortality rate even when advanced disease is present, although prognostic factors and treatment strategy remain uncertain. Between April 2004 and March 2017, 33 juvenile patients (< 30 years old) were diagnosed with DTC and treated at our institution. We retrospectively investigated prognosis and factors including sex, reason for discovery, treatment, pathological factors and treatment progress to clarify the risk factors. All patients underwent curative surgical treatment. Pathologically, lymph node metastasis was identified in 25 patients (75%). Thirteen patients (39%) had bilateral cervical metastasis. In addition, 9 (27%) had more than 10 metastatic lymph nodes. The 2 patients with more than 20 metastatic lymph nodes were treated with radioactive iodine (RAI). Five patients (15%) had local recurrences and received surgery. There have been no further recurrences or deaths. However, no factors were determined to significantly predict the recurrence of juvenile DTC. Local recurrent disease was treated with surgery and/or RAI until remission, and survival was excellent in juvenile DTC. en-copyright= kn-copyright= en-aut-name=TsukiokiTakahiro en-aut-sei=Tsukioki en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShienTadahiko en-aut-sei=Shien en-aut-mei=Tadahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtaniYusuke en-aut-sei=Ohtani en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujiharaMiwa en-aut-sei=Fujihara en-aut-mei=Miwa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SuzukiYoko en-aut-sei=Suzuki en-aut-mei=Yoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KajiharaYukiko en-aut-sei=Kajihara en-aut-mei=Yukiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HatonoMinami en-aut-sei=Hatono en-aut-mei=Minami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawadaKengo en-aut-sei=Kawada en-aut-mei=Kengo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KochiMariko en-aut-sei=Kochi en-aut-mei=Mariko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IwamotoTakayuki en-aut-sei=Iwamoto en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IkedaHirokuni en-aut-sei=Ikeda en-aut-mei=Hirokuni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TairaNaruto en-aut-sei=Taira en-aut-mei=Naruto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=DoiharaHiroyoshi en-aut-sei=Doihara en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=9 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=10 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=11 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=12 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= affil-num=13 en-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital kn-affil= en-keyword=differentiated thyroid carcinoma kn-keyword=differentiated thyroid carcinoma en-keyword=juvenile kn-keyword=juvenile en-keyword=children kn-keyword=children END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=5 article-no= start-page=381 end-page=389 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202010 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical Relevance of Serum Prolactin Levels to Inflammatory Reaction in Male Patients en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the relevance of prolactin (PRL) to clinical parameters in patients who visited our general medicine department, medical records of 353 patients in whom serum PRL levels were measured during the period from 2016 to 2018 were retrospectively reviewed. Data for 140 patients (M/F: 42/98) were analyzed after excluding patients lacking detailed records and patients taking dopaminergic agents. Median serum PRL levels were significantly lower in males than females: 6.5 ng/ml (IQR: 4.2-10.3) versus 8.1 ng/ml (5.9-12.9), respectively. Pain and general fatigue were the major symptoms at the first visit, and past histories of hypertension and dyslipidemia were frequent. Male patients with relatively high PRL levels (≥ 10 ng/ml) had significantly lower levels of serum albumin and significantly higher levels of serum LDH than those with low PRL (< 10 ng/ml). There were significant correlations of male PRL level with the erythrocyte sedimentation rate (R=0.62), serum LDH level (R=0.39) and serum albumin level (R=−0.52), while the level of serum CRP (R=0.33) showed an insignificant but weak positive correlation with PRL level. Collectively, these results show that PRL levels had gender-specific relevance to various clinical factors, with PRL levels in males being significantly related to inflammatory status. en-copyright= kn-copyright= en-aut-name=YamamotoKoichiro en-aut-sei=Yamamoto en-aut-mei=Koichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HanayamaYoshihisa en-aut-sei=Hanayama en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HasegawaKou en-aut-sei=Hasegawa en-aut-mei=Kou kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TokumasuKazuki en-aut-sei=Tokumasu en-aut-mei=Kazuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyoshiTomoko en-aut-sei=Miyoshi en-aut-mei=Tomoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HagiyaHideharu en-aut-sei=Hagiya en-aut-mei=Hideharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OgawaHiroko en-aut-sei=Ogawa en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ObikaMikako en-aut-sei=Obika en-aut-mei=Mikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ItoshimaKoichi en-aut-sei=Itoshima en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OtsukaFumio en-aut-sei=Otsuka en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Laboratory Medicine, Okayama University Hospital kn-affil= affil-num=10 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=hormones kn-keyword=hormones en-keyword=hyperprolactinemia kn-keyword=hyperprolactinemia en-keyword=inflammation kn-keyword=inflammation en-keyword=pituitary kn-keyword=pituitary en-keyword=prolactin kn-keyword=prolactin END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=4 article-no= start-page=351 end-page=358 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Late Phase/Early Phase Ratio of Pancreatic CT Values as a Novel Predictor of Pancreatic Fistula after Distal Pancreatectomy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Post-operative pancreatic fistula (POPF) remains the most common complication after distal pancreatectomy (DP). In this retrospective study, we reviewed the data from patients who underwent DP between 2008 and 2019 in our institute to determine whether the late phase/early phase ratio (L/E ratio) by preoperative computed tomography (CT) scan in the pancreas could predict POPF occurrence after DP. We examined the relationship between preoperative or intraoperative factors and the occurrence of POPF after DP using statistical methods in 23 males and 21 females with a mean age of 73. The mean L/E ratio was significantly lower in the POPF group than the non-POPF group (p=0.035). The L/E ratio had moderate diagnostic accuracy, with a calculated optimal cutoff value of 0.77. In univariate analysis, a significant association was noted between POPF and stump thickness ≥ 16.9, body mass index ≥ 27.5, and L/E ratio ≤ 0.77. In the multivariate analysis, the L/E ratio (odds ratio, 5.96; p=0.036) was an independent risk factor for POPF. Our findings suggest that the pancreatic L/E ratio may predict the occurrence of POPF after DP. This measure may be useful in preoperative risk stratification, patient counseling, and perioperative patient management, improving clinical outcomes after DP. en-copyright= kn-copyright= en-aut-name=UtsumiMasashi en-aut-sei=Utsumi en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AokiHideki en-aut-sei=Aoki en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YabukiTakayuki en-aut-sei=Yabuki en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagahisaSeiichi en-aut-sei=Nagahisa en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishimuraSeitaro en-aut-sei=Nishimura en-aut-mei=Seitaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UneYuta en-aut-sei=Une en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KimuraYuji en-aut-sei=Kimura en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=WatanabeMegumi en-aut-sei=Watanabe en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TaniguchiFumitaka en-aut-sei=Taniguchi en-aut-mei=Fumitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ArataaTakashi en-aut-sei=Arataa en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KatsudaKoh en-aut-sei=Katsuda en-aut-mei=Koh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TanakayaKohji en-aut-sei=Tanakaya en-aut-mei=Kohji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=SatoYumiko en-aut-sei=Sato en-aut-mei=Yumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=2 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=3 en-affil=Department of Radiology, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=4 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=5 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=6 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=7 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=8 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=9 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=10 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=11 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=12 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=13 en-affil=Department of Pathology, National Hospital Organization, Iwakuni Clinical Center kn-affil= en-keyword=late phase/early phase ratio kn-keyword=late phase/early phase ratio en-keyword=pancreatic fistula kn-keyword=pancreatic fistula en-keyword=distal pancreatectomy kn-keyword=distal pancreatectomy END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=4 article-no= start-page=335 end-page=343 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Recurrence of Solitary Fibrous Tumor/Hemangiopericytoma Could Be Predicted by Ki-67 Regardless of Its Origin en-subtitle= kn-subtitle= en-abstract= kn-abstract=Since the discovery of the NAB2-STAT6 gene fusion in 2013, solitary fibrous tumor (SFT) and hemangiopericytoma (HPC) have been considered the same disease. STAT6 nuclear stain is approved as a highly sensitive and specific marker to diagnose SFT/HPC from other tumors with similar histology. As the next step, detection of fusion variants that may predict clinical malignancy of SFT/HPC has been attempted. However, no fusion variants with a clear relation to malignancy have been identified. In this study, the clinical and histological backgrounds of 23 Japanese patients diagnosed with SFT/HPC from 2000 to 2019 at Kochi University Hospital were examined to identify factors potentially related to recurrence. A significant relationship to recurrence was detected for mitosis ≥ 1/10 HPF (400×), necrosis, and Ki-67>5%. These findings indicate that a deliberate investigation of histological features such as mitosis and necrosis is crucial for the clinical observation of SFT/ HPC patients. In addition, Ki-67 was revealed to be a useful parameter to predict recurrence in SFT/HPC patients. en-copyright= kn-copyright= en-aut-name=YamamotoYumiko en-aut-sei=Yamamoto en-aut-mei=Yumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HayashiYoshihiro en-aut-sei=Hayashi en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiIchiro en-aut-sei=Murakami en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Diagnostic Pathology, Kochi University kn-affil= affil-num=2 en-affil=Department of Equipment of Support Planning Office, Kochi University kn-affil= affil-num=3 en-affil=Department of Diagnostic Pathology, Kochi University kn-affil= en-keyword=solitary fibrous tumor kn-keyword=solitary fibrous tumor en-keyword=hemangiopericytoma kn-keyword=hemangiopericytoma en-keyword=Ki-67 kn-keyword=Ki-67 en-keyword=NAB2-STAT6 kn-keyword=NAB2-STAT6 en-keyword=WHO classification kn-keyword=WHO classification en-keyword=WHO grading criteria kn-keyword=WHO grading criteria en-keyword=Marseille Grading System kn-keyword=Marseille Grading System END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=4 article-no= start-page=327 end-page=334 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cytotoxic Effects of Alcohol Extracts from a Plastic Wrap (Polyvinylidene Chloride) on Human Cultured Liver Cells and Mouse Primary Cultured Liver Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=An increasing accumulation of microplastics and further degraded nanoplastics in our environment is suspected to have harmful effects on humans and animals. To clarify this problem, we tested the cytotoxicity of two types of plastic wrap on human cultured liver cells and mouse primary cultured liver cells. Alcohol extracts from plastic wrap, i.e., polyvinylidene chloride (PVDC), showed cytotoxic effects on the cells. Alcohol extracts of polyethylene (PE) wrap were not toxic. The commercially available PVDC wrap consists of vinylidene chloride, epoxidized soybean oil, epoxidized linseed oil as a stiffener and stabilizer; we sought to identify which component(s) are toxic. The epoxidized soybean oil and epoxidized linseed oil exerted strong cytotoxicity, but the plastic raw material itself, vinylidene chloride, did not. Our findings indicate that plastic wraps should be used with caution in order to prevent health risks. en-copyright= kn-copyright= en-aut-name=YamamotoKen-ichi en-aut-sei=Yamamoto en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KagawaHiroko en-aut-sei=Kagawa en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArimotoSakae en-aut-sei=Arimoto en-aut-mei=Sakae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanXian Wen en-aut-sei=Tan en-aut-mei=Xian Wen kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YasuiKento en-aut-sei=Yasui en-aut-mei=Kento kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OshikiToshiyuki en-aut-sei=Oshiki en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SakaguchiMasakiyo en-aut-sei=Sakaguchi en-aut-mei=Masakiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Okayama University Hospital kn-affil= affil-num=3 en-affil=Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Cell Chemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=7 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=plastic wrap kn-keyword=plastic wrap en-keyword=plasticizer, kn-keyword=plasticizer, en-keyword=cytotoxicity, kn-keyword=cytotoxicity, en-keyword=liver cells kn-keyword=liver cells en-keyword=in vitro kn-keyword=in vitro END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=4 article-no= start-page=319 end-page=325 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship Between Partial Carbon Dioxide Pressure and Strong Ions in Humans: A Retrospective Study en-subtitle= kn-subtitle= en-abstract= kn-abstract=Little is known about the role of a strong ions in humans with respiratory abnormalities. In this study, we investigated the associations between partial carbon dioxide pressure (pCO2) and each of sodium ion (Na+) concentrations, chloride ion (Cl−) concentrations and their difference (SIDNa-Cl). Blood gas data were obtained from patients in a teaching hospital intensive care unit between August 2013 and January 2017. The association between pCO2 and SIDNa-Cl was defined as the primary outcome. The associations between pCO2 and [Cl−], [Na+] and other strong ions were secondary outcomes. pCO2 was stratified into 10 mmHg-wide bands and treated as a categorical variable for comparison. As a result, we reviewed 115,936 blood gas data points from 3,840 different ICU stays. There were significant differences in SIDNa-Cl, [Cl−], and [Na+] among all categorized pCO2 bands. The respective pCO2 SIDNa-Cl, [Cl−], and [Na+] correlation coefficients were 0.48, −0.31, and 0.08. SIDNa-Cl increased and [Cl−] decreased with pCO2, with little relationship between pCO2 and [Na+] across subsets. In conclusion, we found relatively strong correlations between pCO2 and SIDNa-Cl in the multiple blood gas datasets examined. Correlations between pCO2 and chloride concentrations, but not sodium concentrations, were further found to be moderate in these ICU data. en-copyright= kn-copyright= en-aut-name=IsoyamaSatoshi en-aut-sei=Isoyama en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KimuraSatoshi en-aut-sei=Kimura en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MorimatsuHiroshi en-aut-sei=Morimatsu en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Anesthesiology and Resuscitation, Okayama University Hospital kn-affil= en-keyword=acid-base phenomena kn-keyword=acid-base phenomena en-keyword=Stewart approach kn-keyword=Stewart approach en-keyword=strong ion difference kn-keyword=strong ion difference en-keyword=chlorine ion kn-keyword=chlorine ion en-keyword=partial carbon dioxide pressure kn-keyword=partial carbon dioxide pressure END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=4 article-no= start-page=265 end-page=274 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202008 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Factors Predicting a Favorable Disease Course Without Anti-TNF Therapy in Crohn’s Disease Patients en-subtitle= kn-subtitle= en-abstract= kn-abstract=Determining factors that predict a favorable disease course without anti-tumor necrosis factor (TNF) agents would help establish a more cost-effective strategy for Crohn’s disease (CD). A retrospective chart review was performed for CD patients with disease durations > 10 years who had not received anti-TNF agents as first-line therapy. Patients were divided into 2 groups: those who received neither anti-TNF agents nor bowel resection (G1), and those who had received an anti-TNF agent and/or bowel resection (G2). The patient backgrounds, therapies and clinical courses were compared between the groups. A total of 62 CD patients met the inclusion criteria (males: 71%; median duration of follow-up: 19 years). Six patients were included in G1; they were significantly less likely to have upper gastrointestinal lesions than G2 (p=0.007). A multivariate analysis revealed that the significant factors for avoidance of bowel resection without anti-TNF treatment were non-stricturing and non-penetrating behaviors, and absence of upper gastrointestinal lesions at the diagnosis (hazard ratios 0.41 and 0.52; p=0.004 and 0.04, respectively). In consideration of the long treatment course of CD, patients with non-stricturing and non-penetrating behaviors and no upper gastrointestinal lesions should not be treated with anti-TNF agents as first-line therapy. en-copyright= kn-copyright= en-aut-name=InokuchiToshihiro en-aut-sei=Inokuchi en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiraokaSakiko en-aut-sei=Hiraoka en-aut-mei=Sakiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YasutomiEriko en-aut-sei=Yasutomi en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OkaShohei en-aut-sei=Oka en-aut-mei=Shohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YamasakiYasushi en-aut-sei=Yamasaki en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KinugasaHideaki en-aut-sei=Kinugasa en-aut-mei=Hideaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakaharMasahiro en-aut-sei=Takahar en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawanoSeiji en-aut-sei=Kawano en-aut-mei=Seiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HaradaKeita en-aut-sei=Harada en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KatoJun en-aut-sei=Kato en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=11 en-affil=bDepartment of Gastroenterology, Mitsui Memorial Hospital kn-affil= en-keyword=Crohn’s disease kn-keyword=Crohn’s disease en-keyword=anti-TNF agent kn-keyword=anti-TNF agent en-keyword=upper gastrointestinal lesion kn-keyword=upper gastrointestinal lesion en-keyword=bamboo joint-like appearance kn-keyword=bamboo joint-like appearance END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=3 article-no= start-page=83 end-page=92 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200421 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Early Decline of alpha-Fetoprotein and Des-gamma-Carboxy Prothrombin Predicts the Response of Hepatic Arterial Infusion Chemotherapy in Hepatocellular Carcinoma Patients en-subtitle= kn-subtitle= en-abstract= kn-abstract=Introduction: Molecular targeting drugs are recommended as second-line treatment for intrahepatic advanced hepatocellular carcinoma (HCC). However, in Asia, hepatic arterial infusion chemotherapy (HAIC) is also considered as a second-line treatment because it improves the survival of responders. The aim of this study was to predict responders and non-responders to HAIC with low-dose cisplatin plus 5-fluorouracil (LFP) using tumor markers.
Objective and Methods: The data of 47 patients who received LFP for the first time in our hospital were analyzed retrospectively. We evaluated the association between treatment response by Response Evaluation Criteria in Solid Tumors and the changing ratio of the serum concentration of alpha-fetoprotein (AFP),Lens culinarisagglutinin-reactive fraction of AFP (AFP-L3), and des-gamma-carboxy prothrombin (DCP) 2 weeks after LFP initiation.
Results: The number of patients showing a complete response (CR), a partial response (PR), stable disease (SD), and progressive disease (PD) was 0 (0%), 20 (43%), 18 (38%), and 9 (19%), respectively. The AFP ratio showed significant positive correlations for PR vs. SD (p= 0.004) and PR vs. PD (p= 0.003). The DCP ratio correlated significantly for PR vs. SD (p= 0.02). The optimal cutoff values for responders were 0.79 for the AFP ratio and 0.53 for the DCP ratio. Prediction using both or either cutoff value showed 93% sensitivity, 53% specificity, a 94% negative predictive value, and a 57% positive predictive value.
Conclusion: Optimal cutoff values for AFP and DCP ratios enable prediction of nonresponders to HAIC with LFP. This simple and early assessment method allows the use of HAIC and molecular targeting drugs for HCC treatment. en-copyright= kn-copyright= en-aut-name=YamamotoShumpei en-aut-sei=Yamamoto en-aut-mei=Shumpei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OnishiHideki en-aut-sei=Onishi en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakakiAkinobu en-aut-sei=Takaki en-aut-mei=Akinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OyamaAtsushi en-aut-sei=Oyama en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AdachiTakuya en-aut-sei=Adachi en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WadaNozomu en-aut-sei=Wada en-aut-mei=Nozomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SakataMasahiro en-aut-sei=Sakata en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YasunakaTetsuya en-aut-sei=Yasunaka en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ShirahaHidenori en-aut-sei=Shiraha en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=Hepatocellular carcinoma kn-keyword=Hepatocellular carcinoma en-keyword=Hepatic arterial infusion chemotherapy kn-keyword=Hepatic arterial infusion chemotherapy en-keyword=Low-dose cisplatin plus 5-fluorouracil kn-keyword=Low-dose cisplatin plus 5-fluorouracil en-keyword=alpha-Fetoprotein kn-keyword=alpha-Fetoprotein en-keyword=Des-gamma-carboxy prothrombin kn-keyword=Des-gamma-carboxy prothrombin END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue= article-no= start-page=113 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pembrolizumab-induced hypothyroidism caused reversible increased serum creatinine levels: a case report en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background
The advent of immune checkpoint inhibitors (ICIs) has significantly improved the prognosis of patients with advanced malignancies. On the other hand, these drugs might cause immune-related adverse events (irAEs) including endocrinopathies and nephropathies. Thyroid dysfunction is one of the most common irAEs. For ICIs-induced nephropathies, most cases are due to tubulointerstitial nephritis, which might require steroid treatment. Here, we report a patient with non-small cell lung cancer treated with ICI who developed increased serum creatinine (s-Cr) levels due to ICIs-induced hypothyroidism.
Case presentation
A 57-year-old Asian man with refractory non-small cell lung cancer under ICIs therapy (pembrolizumab, an anti-programmed cell death-1 monoclonal antibody) developed increased s-Cr levels 5 months after the pembrolizumab initiation. His laboratory data, renal biopsy, and Gallium-67 scintigraphy findings denied pembrolizumab-induced tubulointerstitial nephritis. His renal function was correlated with thyroid function. Despite the increase of s-Cr levels, serum cystatin C levels were normal, which could be explained by the hypothyroidism. Levothyroxine treatment improved renal function as well as thyroid function. Then pembrolizumab was resumed, and both his thyroid and renal function remained normal level. Ultimately, we concluded that the increased s-Cr levels were caused by pembrolizumab-induced hypothyroidism.
Conclusion
All clinicians involved in ICI treatment need to recognize the possible increase in s-Cr levels caused by ICIs-induced hypothyroidism, and we propose monitoring serum cystatin C levels to differentiate ICIs-induced hypothyroidism from tubulointerstitial nephritis before invasive renal biopsies or steroid treatment, which are recommended by the prescribing information for pembrolizumab, are performed. en-copyright= kn-copyright= en-aut-name=MatsuokaNatsumi en-aut-sei=Matsuoka en-aut-mei=Natsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujiKenji en-aut-sei=Tsuji en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IchiharaEiki en-aut-sei=Ichihara en-aut-mei=Eiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HaraTakayuki en-aut-sei=Hara en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FukushimaKazuhiko en-aut-sei=Fukushima en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TomaKishio en-aut-sei=Toma en-aut-mei=Kishio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KitamuraShinji en-aut-sei=Kitamura en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=InagakiKenichi en-aut-sei=Inagaki en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SugiyamaHitoshi en-aut-sei=Sugiyama en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Hematology, Oncology, Allergy and Respiratory Medicine,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Human Resource Development of Dialysis Therapy for Kidney Disease, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=Pembrolizumab kn-keyword=Pembrolizumab en-keyword=Hypothyroidism kn-keyword=Hypothyroidism en-keyword=Creatinine kn-keyword=Creatinine en-keyword=Cystatin C kn-keyword=Cystatin C END start-ver=1.4 cd-journal=joma no-vol=790 cd-vols= no-issue= article-no= start-page=139418 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Microstructure-dependent hydrogen diffusion and trapping in high-tensile steel en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this work, the hydrogen embrittlement (HE) characteristics of high-tensile steel sheets with different microstructural characteristics were investigated. The sheets were fabricated via cold rolling (CR), water quenching (WQ), baking hardening (BH), and low-temperature annealing (LT), and their HE characteristics were clarified by examining the relationships between the microstructural characteristics and the severity of HE. Severe HE occurred in the WQ sample with hydrogen trapping at the boundaries of the retained austenite phases, resulting in intergranular and cleavage-like brittle failure. A reduction in HE was realized after the BH and LT processes. In these cases, hydrogen trapping was divided between the ε-carbide in the lattice spacings and at the boundaries of retained austenite, resulting in a mixed ductile/brittle failure mode. The extent of HE in the CR sample was similar to those in the BH and LT samples. However, the trapping sites were different; hydrogen trapping in the CR sample occurred in the slip band and around dislocations, resulting in delamination-like brittle failure on the slip planes. The extent of HE was also affected by the strain rate. More severe HE occurred in both the WQ and BH samples loaded slowly at 0.01 mm min−1 compared to the samples loaded 1.0 mm min−1 (i.e., intergranular failure). In this case, HE was affected by the large amount of hydrogen atoms trapped at the boundaries of the retained austenite phases. The hydrogen atoms in the lattice structure and ε-carbide migrated to the boundaries via dislocation movement. The extent of deterioration in tensile strength was two times higher in the samples loaded at the higher speed of 1.0 mm min−1 compared to those loaded at 0.01 mm min−1. Finally, the hydrogen trapping and failure mechanisms on the nano and atomic scales were discussed based on the results of the microstructural analyses. en-copyright= kn-copyright= en-aut-name=OkayasuMitsuhiro en-aut-sei=Okayasu en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MotojimaJun en-aut-sei=Motojima en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= en-keyword=High-tensile steel kn-keyword=High-tensile steel en-keyword=Hydrogen embrittlement kn-keyword=Hydrogen embrittlement en-keyword=Hydrogen trapping kn-keyword=Hydrogen trapping en-keyword=Hydrogen diffusion kn-keyword=Hydrogen diffusion en-keyword=Carbide kn-keyword=Carbide en-keyword=Lattice structure kn-keyword=Lattice structure END start-ver=1.4 cd-journal=joma no-vol=138 cd-vols= no-issue= article-no= start-page=105654 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Enhanced expression of nicotinamide nucleotide transhydrogenase (NNT) and its role in a human T cell line continuously exposed to asbestos en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of asbestos fibers on human immune cells have not been well documented. We have developed a continuously exposed cell line model using the human T-lymphotropic virus 1 (HTLV-1)-immortalized human T cell line MT-2. Sublines continuously exposed to chrysotile (CH) or crocidolite (CR) showed acquired resistance to asbestos-induced apoptosis following transient and high-dose re-exposure with fibers. These sublines in addition to other immune cells such as natural killer cells or cytotoxic T lymphocytes exposed to asbestos showed a reduction in anti-tumor immunity. In this study, the expression of genes and molecules related to antioxidative stress was examined. Furthermore, complexes related to oxidative phosphorylation were investigated since the production of reactive oxygen species (ROS) is important when considering the effects of asbestos in carcinogenesis and the mechanisms involved in resistance to asbestos-induced apoptosis. In sublines continuously exposed to CH or CR, the expression of thioredoxin decreased. Interestingly, nicotinamide nucleotide transhydrogenase (NNT) expression was markedly enhanced. Thus, knockdown of NNT was then performed. Although the knockdown clones did not show any changes in proliferation or occurrence of apoptosis, these clones showed recovery of ROS production with returning NADPH/NADP+ ratio that increased with decreased production of ROS in continuously exposed sublines. These results indicated that NNT is a key factor in preventing ROS-induced cytotoxicity in T cells continuously exposed to asbestos. Considering that these sublines showed a reduction in anti-tumor immunity, modification of NNT may contribute to recovery of the anti-tumor effects in asbestos-exposed T cells. en-copyright= kn-copyright= en-aut-name=YamamotoShoko en-aut-sei=Yamamoto en-aut-mei=Shoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=LeeSuni en-aut-sei=Lee en-aut-mei=Suni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuzakiHidenori en-aut-sei=Matsuzaki en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Kumagai-TakeiNaoko en-aut-sei=Kumagai-Takei en-aut-mei=Naoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YoshitomeKei en-aut-sei=Yoshitome en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SadaNagisa en-aut-sei=Sada en-aut-mei=Nagisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShimizuYurika en-aut-sei=Shimizu en-aut-mei=Yurika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ItoTastsuo en-aut-sei=Ito en-aut-mei=Tastsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NishimuraYasumitsu en-aut-sei=Nishimura en-aut-mei=Yasumitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OtsukiTakemi en-aut-sei=Otsuki en-aut-mei=Takemi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=2 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=3 en-affil=Department of Life Science, Faculty of Life and Environmental Science, Prefectural University of Hiroshima kn-affil= affil-num=4 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Biophysical Chemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=9 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= affil-num=10 en-affil=Department of Hygiene, Kawasaki Medical School kn-affil= en-keyword=Asbestos kn-keyword=Asbestos en-keyword=Continuous exposure kn-keyword=Continuous exposure en-keyword=Oxidative phosphorylation kn-keyword=Oxidative phosphorylation en-keyword=T cell kn-keyword=T cell en-keyword=nicotinamide nucleotide transhydrogenase (NNT) kn-keyword=nicotinamide nucleotide transhydrogenase (NNT) END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page=171 end-page=174 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Incarcerated Amyand’s Hernia en-subtitle= kn-subtitle= en-abstract= kn-abstract= A 76-year-old Japanese man was transferred to our hospital to undergo rehabilitation after traffic accident-related injuries. Seven days post-admission, he presented with abdominal pain and an 8-cm lump in the right inguinal region. He was diagnosed with an incarcerated inguinal hernia and underwent elective surgery the day after manual reduction. He had a normal vermiform appendix which was observed to have adhered to the right indirect hernia sac. An appendectomy and hernia repair using lightweight mesh were performed. We discuss the surgical management of this rare incarcerated Amyand’s hernia and the relevant literature. en-copyright= kn-copyright= en-aut-name=OkitaAtsushi en-aut-sei=Okita en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshidaOsamu en-aut-sei=Yoshida en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiMasakazu en-aut-sei=Murakami en-aut-mei=Masakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Surgery, Yakage Town National Health Insurance Hosptial kn-affil= affil-num=2 en-affil=Department of Surgery, Yakage Town National Health Insurance Hosptial kn-affil= affil-num=3 en-affil=Department of Surgery, Yakage Town National Health Insurance Hosptial kn-affil= en-keyword=Amyand’s hernia kn-keyword=Amyand’s hernia en-keyword=incarcerated inguinal hernia kn-keyword=incarcerated inguinal hernia en-keyword=appendectomy kn-keyword=appendectomy END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page=145 end-page=150 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Efficacy of Mirabegron for the Relief of Ureteral Stent-Related Symptoms en-subtitle= kn-subtitle= en-abstract= kn-abstract= To investigate the efficacy of mirabegron for lower urinary tract symptoms in patients with an indwelling ureteral stent after ureterorenoscopic lithotripsy. This was a prospective follow-up study of 76 patients with stent-related symptoms (SRSs). Patients with upper urinary calculi who were pre-stented for > 2 weeks before lithotripsy were examined for the presence of SRSs by tests including the International Prostate Symptom Score (IPSS), OAB Symptom Score (OABSS), and urinary bother and pain measured by a Visual Analogue Scale (VAS) before lithotripsy. Mirabegron (50 mg/day) was prescribed post-lithotripsy for 2 weeks. SRSs were assessed at the time of stent removal. The IPSS scores improved significantly from 16.2 to 14.3 (p<0.001) and the IPSS-QoL scores decreased significantly from 5.0 to 4.6 (p=0.012). The OABSS scores improved significantly from 7.7 to 6.8 (p=0.006), and the urinary urgency scores (OABSS-Q3) decreased significantly from 3.24 to 2.68 (p<0.001). The number of nocturia episodes decreased significantly from 2.5 to 2.2 (p=0.045). Urinary bother and pain assessed by the VAS declined from 4.2 and 3.1 to 3.8 (p=0.15) and 2.5 (p=0.075), respectively. Mirabegron significantly improved SRSs and the number of nocturia episodes due to a ureteral stent. en-copyright= kn-copyright= en-aut-name=OtsukiHideo en-aut-sei=Otsuki en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamasakiTomoya en-aut-sei=Yamasaki en-aut-mei=Tomoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HoriShunsuke en-aut-sei=Hori en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AokiHiroshi en-aut-sei=Aoki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KosakaTakeo en-aut-sei=Kosaka en-aut-mei=Takeo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UeharaShinya en-aut-sei=Uehara en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FujioKei en-aut-sei=Fujio en-aut-mei=Kei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Urology, Abiko Toho Hospital kn-affil= affil-num=2 en-affil=Department of Urology, Abiko Toho Hospital kn-affil= affil-num=3 en-affil=Department of Urology, Abiko Toho Hospital kn-affil= affil-num=4 en-affil=Department of Urology, Abiko Toho Hospital kn-affil= affil-num=5 en-affil=Department of Urology, Keio University kn-affil= affil-num=6 en-affil=Department of Urology, Kawasaki Medical School General Medical Center kn-affil= affil-num=7 en-affil=Department of Urology, Abiko Toho Hospital kn-affil= en-keyword=stent-related symptoms kn-keyword=stent-related symptoms en-keyword=overactive bladder kn-keyword=overactive bladder en-keyword=mirabegron kn-keyword=mirabegron en-keyword=ureterorenoscopic lithotripsy kn-keyword=ureterorenoscopic lithotripsy en-keyword=ureteral stent kn-keyword=ureteral stent END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page=137 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mucinous Carcinoma of the Breast: Clinicopathological Features and Long-term Prognosis in Comparison with Invasive Ductal Cancer; A Single Hospital’s 30+-Year Experience en-subtitle= kn-subtitle= en-abstract= kn-abstract= Mucinous carcinoma (MC) of the breast is a rare and special type of malignancy, with a substantial amount of extracellular mucin. We compared the clinicopathological features and the long-term survival of MC patients with those of invasive ductal carcinoma-no special type (IDC-NST) patients, and we examined prognostic factors of MC. A total of 116 patients with mucinous carcinoma and 3,258 patients with IDC-NST who underwent surgery at our hospital (1977-2008) were enrolled. The 10-year overall survival rate and breast cancer-specific survival rate (BSS) of the MC patients (88.3%, 93.7%) were both significantly higher than those of IDC-NST patients (81.6%, 85.0%) (p=0.015, p=0.005, respectively). A Cox regression analysis demonstrated that MC tended to be an independent prognostic factor (hazard ratio 0.44, p=0.098). The BSS of the MC patients with positive lymph node (LN) metastasis was significantly poorer than that of the patients without it, by univariate analysis (p=0.002). The tumor size in the MC patients with positive LN metastasis (mean 3.2 cm) was significantly larger than that in the patients without it (mean 1.9 cm) (p=0.0004). Although a Cox regression analysis revealed no independent factor, MC patients with positive LN metastasis should be treated for advanced invasive ductal breast cancer. en-copyright= kn-copyright= en-aut-name=IsozakiHiroshi en-aut-sei=Isozaki en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoYasuhisa en-aut-sei=Yamamoto en-aut-mei=Yasuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SakaiKunihiko en-aut-sei=Sakai en-aut-mei=Kunihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShoTatuo en-aut-sei=Sho en-aut-mei=Tatuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IshiharaKiyohiro en-aut-sei=Ishihara en-aut-mei=Kiyohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiShigeki en-aut-sei=Murakami en-aut-mei=Shigeki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MatsumotoSasau en-aut-sei=Matsumoto en-aut-mei=Sasau kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakamaTakehiro en-aut-sei=Takama en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=2 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=3 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=4 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=5 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=6 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=7 en-affil=Department of Surgery, Oomoto Hospital kn-affil= affil-num=8 en-affil=Department of Surgery, Oomoto Hospital kn-affil= en-keyword=breast cancer kn-keyword=breast cancer en-keyword=mucinous carcinoma kn-keyword=mucinous carcinoma en-keyword=clinicopathological features kn-keyword=clinicopathological features en-keyword=long-term prognosis kn-keyword=long-term prognosis END start-ver=1.4 cd-journal=joma no-vol=74 cd-vols= no-issue=2 article-no= start-page=129 end-page=135 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=202004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association between Histological Types and Enhancement of Dynamic CT for Primary Lung Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract= The aim of this study was to explore enhancement patterns of different types of primary lung cancers on 2-phase dynamic computed tomography (CT). This study included 217 primary lung cancer patients (141 adenocarcinomas [ADs], 48 squamous cell carcinomas [SCCs], 20 small cell lung carcinomas [SCLCs], and 8 others) who were examined using a 2-phase dynamic scan. Regions of interest were identified and mean enhancement values were calculated. After excluding the 20 SCLCs because these lesions had different clinical stages from the other cancer types, the mean attenuation values and subtractions between phases were compared between types of non-small cell lung carcinomas (NSCLCs) using the Kruskal–Wallis test. Late phase attenuation and attenuation of the late minus unenhanced phase (LMU) of SCCs were significantly higher than those of ADs (p<0.05). To differentiate SCC and AD in the late phase, a threshold of 80.21 Hounsfield units (HU) gave 52.9% accuracy. In LMU, a threshold of 52.16 HU gave 59.3% accuracy. Dynamic lung CT has the potential to aid in differentiating among NSCLC types. en-copyright= kn-copyright= en-aut-name=FukumaShogo en-aut-sei=Fukuma en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShinyaTakayoshi en-aut-sei=Shinya en-aut-mei=Takayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SohJunichi en-aut-sei=Soh en-aut-mei=Junichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FukuharaRyuichiro en-aut-sei=Fukuhara en-aut-mei=Ryuichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OgawaNanako en-aut-sei=Ogawa en-aut-mei=Nanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HigakiFumiyo en-aut-sei=Higaki en-aut-mei=Fumiyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IchiharaEiki en-aut-sei=Ichihara en-aut-mei=Eiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=HirakiTakao en-aut-sei=Hiraki en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ToyookaShinichi en-aut-sei=Toyooka en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KanazawaSusumu en-aut-sei=Kanazawa en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Radiology, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of General Thoracic Surgery, Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Pediatric Radiology, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Radiology, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Radiology, Okayama City General Medical Center kn-affil= affil-num=7 en-affil=Department of Pathology, Okayama University Hospital kn-affil= affil-num=8 en-affil=Department of Allergy and Respiratory Medicine, Okayama University Hospital kn-affil= affil-num=9 en-affil=Department of Radiology, Okayama University Hospital kn-affil= affil-num=10 en-affil=Department of General Thoracic Surgery, Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=11 en-affil=Department of Radiology, Okayama University Hospital kn-affil= en-keyword=differentiation kn-keyword=differentiation en-keyword=dynamic computed tomography kn-keyword=dynamic computed tomography en-keyword=primary lung cancer kn-keyword=primary lung cancer en-keyword=enhancement pattern kn-keyword=enhancement pattern END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=4 article-no= start-page=414 end-page=419 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201610 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of prostaglandin D2 on VEGF release by nasal polyp fibroblasts en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background: Vascular endothelial growth factor (VEGF) is known to be associated with the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). VEGF is produced by a variety of cells including fibroblasts. It was recently reported that prostaglandin (PG) E-2 induces VEGF release by nasal polyp fibroblasts. However, little is known regarding possible regulation of VEGF by other PGs. We have reported that molecules that regulate PGD(2) metabolism play roles in the pathogenesis of CRS including in local eosinophilia and type 2 cytokine production. In the present study, we sought to determine whether PGD(2) regulates VEGF release by nasal polyp fibroblasts.
Methods: Nasal polyp fibroblasts were established from nasal polyps. These fibroblasts were stimulated with serial dilutions of PGD(2) or PGD(2) receptor (DP/CRTH2)-selective agonists in the presence or absence of receptor-selective antagonists. The concentration of VEGF in the culture supernatants was determined using ELISA.
Results: 5 mM of PGD(2) significantly induced VEGF release by nasal polyp fibroblasts. VEGF release was also obtained by stimulation with a DP receptor-selective, but not with a CRTH2 receptor-selective agonist. In addition, PGD(2)-induced VEGF release was significantly inhibited by pre-treatment with DP receptor-selective antagonists. In contrast, pre-treatment with a CRTH2 receptor-selective antagonist significantly enhanced PGD(2)-induced VEGF release.
Conclusions: PGD(2) stimulates VEGF production via DP but not CRTH2 receptors in nasal polyp fibroblasts. Copyright (C) 2016, Japanese Society of Allergology. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. en-copyright= kn-copyright= en-aut-name=KanaiKengo en-aut-sei=Kanai en-aut-mei=Kengo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkanoMitsuhiro en-aut-sei=Okano en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KariyaShin en-aut-sei=Kariya en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HarunaTakenori en-aut-sei=Haruna en-aut-mei=Takenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OmichiRyotaro en-aut-sei=Omichi en-aut-mei=Ryotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MakiharaSei-ichiro en-aut-sei=Makihara en-aut-mei=Sei-ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HirataYuji en-aut-sei=Hirata en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department Otorhinolaryngology, Kagawa Rosai Hospital kn-affil= affil-num=8 en-affil=Department Otorhinolaryngology, Kagawa Prefectural Central Hospital kn-affil= affil-num=9 en-affil=Department Otorhinolaryngology, Kagawa Prefectural Central Hospital kn-affil= en-keyword=CRTH2 kn-keyword=CRTH2 en-keyword=DP kn-keyword=DP en-keyword=Nasal polyp fibroblast kn-keyword=Nasal polyp fibroblast en-keyword=PGD2 kn-keyword=PGD2 en-keyword=VEGF kn-keyword=VEGF END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=5 article-no= start-page=779 end-page=789 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190927 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cavity Adaptation of Composite Restorations Prepared at Crown and Root: Optical Assessment Using SS-OCT en-subtitle= kn-subtitle= en-abstract= kn-abstract= Evaluation of gap formation at the interfaces of a two-step self-etching adhesive with/without pre-etching was performed using sweptsource optical coherence tomography (SS-OCT). Round cavities were prepared in bovine incisors at the middle (MC) and cervical (CC) thirds of the crown and the cervical third of the root (CR). Clearfil SE bond was directly applied to one group (SE) and another (PA) was pretreated with K-etchant gel. Following restoration by flowable composite resin, the teeth were thermally challenged and stored for 2 months. Interfacial gaps observed in the cross-sectional OCT images were analyzed and the bottom cavities exhibited increased gaps compared to the margin and dentin-enamel junction (DEJ). The CR site had a larger gap than at MC and CC in the SE group. DEJ separation at the MC was significantly smaller than that at CC in both groups. Therefore, gap formation depends on the cavity region, location, and bonding protocol. en-copyright= kn-copyright= en-aut-name=Rima Zakzuk Alshahni en-aut-sei=Rima Zakzuk Alshahni en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimadaYasushi en-aut-sei=Shimada en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZhouYuan en-aut-sei=Zhou en-aut-mei=Yuan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshiyamaMasahiro en-aut-sei=Yoshiyama en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SadrAlireza en-aut-sei=Sadr en-aut-mei=Alireza kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SumiYasunori en-aut-sei=Sumi en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TagamiJunji en-aut-sei=Tagami en-aut-mei=Junji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University kn-affil= affil-num=2 en-affil=Department of Operative Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University kn-affil= affil-num=4 en-affil=Department of Operative Dentistry, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Restorative Dentistry, University of Washington, School of Dentistry kn-affil= affil-num=6 en-affil=Division of Oral and Dental Surgery, Department of Advanced Medicine, National Center for Geriatrics and Gerontology, National Hospital for Geriatric Medicine kn-affil= affil-num=7 en-affil=Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University kn-affil= en-keyword=Bottom kn-keyword=Bottom en-keyword=Dentin-enamel junction kn-keyword=Dentin-enamel junction en-keyword=Gap kn-keyword=Gap en-keyword=SS-OCT kn-keyword=SS-OCT en-keyword=Self-etch adhesive kn-keyword=Self-etch adhesive END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200114 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Serum IgG4 as a biomarker reflecting pathophysiology and post-operative recurrence in chronic rhinosinusitis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background: Type 2 chronic rhinosinusitis (CRS), especially eosinophilic CRS (ECRS), is an intractable upper airway inflammatory disease. Establishment of serum biomarkers reflecting the pathophysiology of CRS is desirable in a clinical setting. As IgG4 production is regulated by type 2 cytokines, we sought to determine whether serum IgG4 levels can be used as a biomarker for CRS.
Methods: Association between the serum IgG4 levels and clinicopathological factors was analyzed in 336 CRS patients. Receiver operating characteristics (ROC) analysis was performed to determine the cut-off value of serum IgG4 levels that can be used to predict the post-operative recurrence.
Results: Serum IgG4 levels were significantly higher in patients with moderate to severe ECRS versus those with non to mild ECRS. The levels were also significantly higher in asthmatic patients and patients exhibiting recurrence after surgery compared to controls. ROC analysis determined that the best cut-off value for the serum IgG4 level to predict the post-operative recurrence was 95 mg/dL. The corresponding sensitivity and specificity were 39.7% and 80.5%, respectively. When we combined the two cut-off values for the serum IgG4 and periostin, patients with high serum levels of either IgG4 or periostin exhibited a high post-operative recurrence (OR: 3.95) as compared to patients having low serum levels of both IgG4 and periostin.
Conclusions: The present results demonstrate that the serum IgG4 level is associated with disease severity and post-operative course in CRS. In particular, the combination of serum IgG4 and periostin could be a novel biomarker that predicts post-operative recurrence. en-copyright= kn-copyright= en-aut-name=OkaAiko en-aut-sei=Oka en-aut-mei=Aiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NinomiyaTakahiro en-aut-sei=Ninomiya en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SatoYasuharu en-aut-sei=Sato en-aut-mei=Yasuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GionYuka en-aut-sei=Gion en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MinouraAkira en-aut-sei=Minoura en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HarunaShin-ichi en-aut-sei=Haruna en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YoshidaNaohiro en-aut-sei=Yoshida en-aut-mei=Naohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=SakumaYasunori en-aut-sei=Sakuma en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IzuharaKenji en-aut-sei=Izuhara en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OnoJunya en-aut-sei=Ono en-aut-mei=Junya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=TaniguchiMasami en-aut-sei=Taniguchi en-aut-mei=Masami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=HarunaTakenori en-aut-sei=Haruna en-aut-mei=Takenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=HigakiTakaya en-aut-sei=Higaki en-aut-mei=Takaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=KariyaShin en-aut-sei=Kariya en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=KoyamaTakahisa en-aut-sei=Koyama en-aut-mei=Takahisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=TakabayashiTetsuji en-aut-sei=Takabayashi en-aut-mei=Tetsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=ImotoYoshimasa en-aut-sei=Imoto en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=SakashitaMasafumi en-aut-sei=Sakashita en-aut-mei=Masafumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= en-aut-name=KidoguchiMasanori en-aut-sei=Kidoguchi en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=21 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=22 ORCID= en-aut-name=FujiedaShigeharu en-aut-sei=Fujieda en-aut-mei=Shigeharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=23 ORCID= en-aut-name=OkanoMitsuhiro en-aut-sei=Okano en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=24 ORCID= affil-num=1 en-affil=Department of Otorhinolaryngology, International University of Health and Welfare Graduate School of Medicine kn-affil= affil-num=2 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=3 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=6 en-affil=Division of Pathophysiology, Okayama University Graduate School of Health Sciences kn-affil= affil-num=7 en-affil=Department of Hygiene, Public Health and Preventive Medicine, Showa University School of Medicine kn-affil= affil-num=8 en-affil=Department of Otorhinolaryngology, Head & Neck Surgery, Dokkyo Medical University kn-affil= affil-num=9 en-affil=Department of Otolaryngology, Jichi Medical University Saitama Medical Center kn-affil= affil-num=10 en-affil=Department of Otorhinolaryngology, Yokohama City Medical Center kn-affil= affil-num=11 en-affil=Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School kn-affil= affil-num=12 en-affil=Shino-Test Co., Ltd. kn-affil= affil-num=13 en-affil=Clinical Research Center for Allergy and Rheumatology, National Hospital Organization Sagamihara National Hospital kn-affil= affil-num=14 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=15 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=16 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=17 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=18 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=19 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=20 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=21 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=22 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=23 en-affil=Department of Otorhinolaryngology Head & Neck Surgery, Faculty of Medical Sciences, University of Fukui kn-affil= affil-num=24 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Chronic rhinosinusitis kn-keyword=Chronic rhinosinusitis en-keyword=Eosinophils kn-keyword=Eosinophils en-keyword=Eosinophils kn-keyword=Eosinophils en-keyword= IgG4 kn-keyword= IgG4 en-keyword=Severity kn-keyword=Severity en-keyword=Surgery kn-keyword=Surgery END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=4 article-no= start-page=577 end-page=583 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20191014 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mechanical and fatigue properties of long carbon fiber reinforced plastics at low temperature en-subtitle= kn-subtitle= en-abstract= kn-abstract=The mechanical properties of long unidirectional (UD) and crossply (CR) carbon fiber reinforced plastics (CFRPs) were investigated at a low temperature (−196 °C). The CFRPs were fabricated from 60 vol.% carbon fiber and epoxy resin. The bending strength of the UD-CFRP was approximately twice that of the CR-CFRP. The high strength of the UD-CFRP was directly attributed to the amount of carbon fiber oriented along the loading direction: 60% for UD-CFRP compared with 30% for CR-CFRP. The low-temperature (−196 °C) tensile and fatigue strengths of the UD-CFRP were over 1.5 times greater than those at room temperature (20 °C). This was attributed to the increased epoxy strength at low temperatures along with the internal compressive stress arising from the different thermal expansion coefficients of the carbon fiber and epoxy. Both the epoxy strength and internal compressive strength were employed as factors in a compound law to numerically estimate the low-temperature tensile strength. This work presents a systematic analysis for changes in the CFRP material properties at low temperatures. en-copyright= kn-copyright= en-aut-name=OkayasuMitsuhiro en-aut-sei=Okayasu en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsuchiyaYuki en-aut-sei=Tsuchiya en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= en-keyword=CFRP kn-keyword=CFRP en-keyword=Carbon fiber kn-keyword=Carbon fiber en-keyword=Tensile strength kn-keyword=Tensile strength en-keyword=Fatigue strength kn-keyword=Fatigue strength en-keyword=Low temperature kn-keyword=Low temperature END start-ver=1.4 cd-journal=joma no-vol=110 cd-vols= no-issue=5 S2 article-no= start-page=S81 end-page=S87 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201605 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of IAEA Clearance Concept for Low-level Radioactive Waste from a Radioisotope Research Institute en-subtitle= kn-subtitle= en-abstract= kn-abstract=The clearance of solid low-level radioactive laboratory waste (LLRW) after decay-in-storage (DIS) obtained from a research institute and thoroughly separated using the separation and classification protocols presented in this study was evaluated.
METHOD:
The radioisotope (RI) content of incinerated LLRW from the specified RI research group (group A); the RI content of LLRW obtained in fiscal year 2000, which contained radionuclides with half-lives of less than 164 d (LLRW2); and the RI content of the LLRW reported in group A's disposal records were compared. The LLRW2 and LLRW of group A were incinerated after 2 y of decay-in-storage and immediately after storage, respectively.
RESULTS:
The highest ratio of the RI of incinerated LLRW to the value in the disposal records was 2.52 for ⁵¹Cr. The radioactivities of radionuclides in both the LLRW2 and LLRW for ³⁵S, ⁴⁵Ca, ⁵¹Cr, ¹²⁵I, ³²P, ³³P, and ⁹⁹mTc and the incinerated ash after 1 y later of decay-in-storage were below the clearance level defined by the RS-G-1.7 of the International Basic Safety Standard without contamination by ³H and ¹⁴C. These remains contained very small amounts of some long-half-life radionuclides of natural origin after 7 y of decay-in-storage.
CONCLUSION:
This LLRW separation protocol was effective for the separation of ³H and ¹⁴C. LLRW2 after 2 years of DIS and its incinerated ash after one year later of DIS were below the clearance level for radioactivity and radioactivity concentration. en-copyright= kn-copyright= en-aut-name=YumotoYasuhiro en-aut-sei=Yumoto en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkadaShigeru en-aut-sei=Okada en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KinnoIkuo en-aut-sei=Kinno en-aut-mei=Ikuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagamatsuTomohiro en-aut-sei=Nagamatsu en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NousoKazuhiro en-aut-sei=Nouso en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NakayamaEiichi en-aut-sei=Nakayama en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Radiation Research, Shikata Laboratory Okayama University Advanced Science Research Center (RIC) kn-affil= affil-num=2 en-affil=Department of General Medicine, Okayama University Graduate School, Medicine, Dentistry and Pharmacological Research kn-affil= affil-num=3 en-affil=Department of Radiation Research, Shikata Laboratory Okayama University Advanced Science Research Center (RIC) kn-affil= affil-num=4 en-affil=Department of Radiation Research, Shikata Laboratory Okayama University Advanced Science Research Center (RIC) kn-affil= affil-num=5 en-affil=Department of Gastroenterology and Hepatology, Okayama University, Graduate School Medicine, Dentistry and Pharmacological Science kn-affil= affil-num=6 en-affil=Department of Radiation Research, Shikata Laboratory Okayama University Advanced Science Research Center (RIC) kn-affil= en-keyword=operational topics kn-keyword=operational topics en-keyword=waste kn-keyword=waste en-keyword=low-level kn-keyword=low-level en-keyword=waste disposal kn-keyword=waste disposal en-keyword=waste management kn-keyword=waste management END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=5 article-no= start-page=469 end-page=474 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Phase II Trial Using Romidepsin after Gemcitabine, Dexamethasone, and Cisplatin Therapy in Elderly Transplant-Ineligible Patients with Relapsed/Refractory Peripheral T-Cell Lymphoma: Study Protocol en-subtitle= kn-subtitle= en-abstract= kn-abstract= Romidepsin is an important therapeutic option for patients with peripheral T-cell lymphoma (PTCL). However, the timing of romidepsin administration remains controversial. Romidepsin was launched in Japan as a consolidation therapy agent after conventional salvage chemotherapy with gemcitabine, dexamethasone, and cisplatin (GDP). GDP therapy will be administered every 3 weeks. If complete response, partial response, or stable disease is confirmed after 2-4 GDP cycles, romidepsin will be administered every 4 weeks. The primary endpoint is a 2-year progression-free survival rate. Patients participating in this study and undergoing treatment can expect results similar to or better than those of conventional therapies. en-copyright= kn-copyright= en-aut-name=YamasakiSatoshi en-aut-sei=Yamasaki en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KadaAkiko en-aut-sei=Kada en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NagaiHirokazu en-aut-sei=Nagai en-aut-mei=Hirokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshidaIsao en-aut-sei=Yoshida en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ChoiIlseung en-aut-sei=Choi en-aut-mei=Ilseung kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SaitoAkiko M. en-aut-sei=Saito en-aut-mei=Akiko M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IwasakiaHiromi en-aut-sei=Iwasakia en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Hematology and Clinical Research Institute, NHO Kyushu Medical Cente kn-affil= affil-num=2 en-affil=Department of Clinical Trials and Research, Clinical Research Center, NHO Nagoya Medical Center kn-affil= affil-num=3 en-affil=Department ofHematology and Oncology Research, NHO Nagoya Medical Center kn-affil= affil-num=4 en-affil=Department of Hematologic Oncology, NHO Shikoku Cancer Center kn-affil= affil-num=5 en-affil=Department of Hematology, NHO Kyushu Cancer Center kn-affil= affil-num=6 en-affil=Department of Clinical Trials and Research, Clinical Research Center, NHO Nagoya Medical Center kn-affil= affil-num=7 en-affil=Department of Hematology and Clinical Research Institute, NHO Kyushu Medical Cente kn-affil= en-keyword=peripheral T-cell lymphoma not otherwise specified kn-keyword=peripheral T-cell lymphoma not otherwise specified en-keyword=angioimmunoblastic T-cell lymphoma kn-keyword=angioimmunoblastic T-cell lymphoma en-keyword=gemcitabine kn-keyword=gemcitabine en-keyword=cisplatin, romidepsin kn-keyword=cisplatin, romidepsin END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=5 article-no= start-page=387 end-page=392 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparison of Two Different Drugs for Overactive Bladder, Solifenacin and Mirabegron: A Prospective Randomized Crossover Study en-subtitle= kn-subtitle= en-abstract= kn-abstract= To assess the efficacy and safety of 2 drugs for overactive bladder (OAB), solifenacin and mirabegron. Fortyseven female OAB patients were randomized into 2 groups. Twenty-three patients were initially prescribed solifenacin for 4 weeks, followed by mirabegron for 4 weeks (group S). The other 24 patients were initially prescribed mirabegron for 4 weeks, followed by solifenacin for 4 weeks (group M). Evaluations included clinical determination of the OAB symptom score (OABSS), International Prostate Symptom Score (IPSS), and Visual Analog Scale. The IPSS significantly improved after the administration of solifenacin in both groups. The OABSS significantly improved in both groups after 4 weeks. In group M, the OABSS after eight weeks was significantly improved compared to that after 4 weeks. However, in group S, it was not significantly improved. Twelve patients experienced adverse events during the solifenacin treatment, while 2 patients experienced adverse events during the mirabegron treatment. Both solifenacin and mirabegron led to improved OAB symptoms. Switching from mirabegron to solifenacin significantly improved the OABSS. However, mirabegron led to fewer adverse events than solifenacin. We recommend that mirabegron be prescribed first for OAB patients. If patients are not satisfied with mirabegron, solifenacin should be used. en-copyright= kn-copyright= en-aut-name=InoueMiyabi en-aut-sei=Inoue en-aut-mei=Miyabi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YokoyamaTeruhiko en-aut-sei=Yokoyama en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Miyabi Urogyne Clinic kn-affil= affil-num=2 en-affil=Yokoyama Urological Clinic kn-affil= en-keyword=overactive bladder, kn-keyword=overactive bladder, en-keyword=randomized crossover study kn-keyword=randomized crossover study en-keyword=solifenacin kn-keyword=solifenacin en-keyword=mirabegron kn-keyword=mirabegron END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=5 article-no= start-page=379 end-page=382 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histidine-rich Glycoprotein Modulates the Blood-vascular System in Septic Condition en-subtitle= kn-subtitle= en-abstract= kn-abstract= Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 μg/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi. Therefore, reduction of plasma HRG levels in sepsis leads to dysregulation of coagulation, fibrinolysis, and immune response, resulting in disseminated intravascular coagulation and multiple organ failure. This review summarizes the binding and functional properties of HRG in sepsis. en-copyright= kn-copyright= en-aut-name=WakeHidenori en-aut-sei=Wake en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=htidine-rich glycoprotein kn-keyword=htidine-rich glycoprotein en-keyword=septic pathogenesis kn-keyword=septic pathogenesis en-keyword=immunothrombosis kn-keyword=immunothrombosis END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=2 article-no= start-page=216 end-page=224 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Significance of IgG4-positive cells in severe eosinophilic chronic rhinosinusitis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background: IgG4 production is regulated by type 2 (IL-4 and IL-13) and regulatory (IL-10) cytokines involved in the pathophysiology of chronic rhinosinusitis (CRS). We sought to determine the pathophysiological characteristics of IgG4-positive cells in sinonasal tissues in CRS, especially eosinophilic CRS (ECRS).
Methods: IgG4-positive cells in uncinate tissues (UT) and nasal polyps (NP) were examined by immunohistochemistry. Associations between the number of IgG4-positive cells and clinicopathological factors were analyzed. Receiver operating characteristics (ROC) analysis was performed to determine the cut-off value of IgG4-positive cells in tissue that can predict the post-operative course.
Results: IgG4 was mainly expressed in infiltrating plasma and plasmacytoid cells, and the number of IgG4-positive cells was significantly higher in NP, especially those from severe ECRS patients, than in UT. In CRS patients, the number of IgG4-positive cells significantly and positively correlated with blood and tissue eosinophilia, radiological severity, and serum level of total IgE. The number of infiltrating IgG4-positive cells was significantly higher in patients with a poor post-operative course (sustained sinus shadow 6 months after surgery) than in those with a good one. The number of IgG4-positive cells in NP could discriminate patients with a good or a poor post-operative course (area under the curve: 0.769). Also, 73.3% sensitivity and 82.5% specificity were achieved when the cut-off value was set at 17 cells/high-power field.
Conclusions: Our results suggest that the local expression of IgG4 on cells may be used as a biomarker that reflects the pathophysiology of CRS, including the post-operative course. en-copyright= kn-copyright= en-aut-name=KoyamaTakahisa en-aut-sei=Koyama en-aut-mei=Takahisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KariyaShin en-aut-sei=Kariya en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoYasuharu en-aut-sei=Sato en-aut-mei=Yasuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GionYuka en-aut-sei=Gion en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HigakiTakaya en-aut-sei=Higaki en-aut-mei=Takaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HarunaTakenori en-aut-sei=Haruna en-aut-mei=Takenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FujiwaraTazuko en-aut-sei=Fujiwara en-aut-mei=Tazuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MinouraAkira en-aut-sei=Minoura en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TakaoSoshi en-aut-sei=Takao en-aut-mei=Soshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OritaYorihisa en-aut-sei=Orita en-aut-mei=Yorihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KanaiKengo en-aut-sei=Kanai en-aut-mei=Kengo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=TaniguchiMasami en-aut-sei=Taniguchi en-aut-mei=Masami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NishizakiKazunori en-aut-sei=Nishizaki en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=OkanoMitsuhiro en-aut-sei=Okano en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Public Health, Showa University School of Medicine kn-affil= affil-num=9 en-affil=Department of Epidemiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Otolaryngology-Head and Neck Surgery, Kumamoto University kn-affil= affil-num=11 en-affil=Department of Otorhinolaryngology-Head & Neck Surgery, Kagawa Prefectural Central Hospital kn-affil= affil-num=12 en-affil=Clinical Research Center for Allergy and Rheumatology, National Hospital Organization Sagamihara National Hospital kn-affil= affil-num=13 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=14 en-affil=Department of Otolaryngology-Head & Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=Chronic rhinosinusitis kn-keyword=Chronic rhinosinusitis en-keyword=Eosinophils kn-keyword=Eosinophils en-keyword=IgG4 kn-keyword=IgG4 en-keyword=Nasal polyps kn-keyword=Nasal polyps en-keyword=Severity kn-keyword=Severity END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=4 article-no= start-page=315 end-page=323 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis: Evidence of the Contribution of PGN_0297 to Gingipain Activity en-subtitle= kn-subtitle= en-abstract= kn-abstract=The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism’s virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains. en-copyright= kn-copyright= en-aut-name=OnoShintaro en-aut-sei=Ono en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakayamaMasaaki en-aut-sei=Nakayama en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TachibanaMasato en-aut-sei=Tachibana en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Abu Saleh Muhammad Shahriar en-aut-sei=Abu Saleh Muhammad Shahriar en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HelingWang en-aut-sei=Heling en-aut-mei=Wang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakashibaShogo en-aut-sei=Takashiba en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OharaNaoya en-aut-sei=Ohara en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=periodontitis kn-keyword=periodontitis en-keyword=Porphyromonas gingivalis kn-keyword=Porphyromonas gingivalis en-keyword=gingipain kn-keyword=gingipain en-keyword=C-terminal domain kn-keyword=C-terminal domain en-keyword=secretion system kn-keyword=secretion system END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=4 article-no= start-page=299 end-page=305 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tuberosity-overlapping Fixation of the Humeral Shaft in Humeral Head Replacement Surgery en-subtitle= kn-subtitle= en-abstract= kn-abstract= The fixation technique of bony fragments is crucial for the bone union of the tuberosities after humeral head replacement (HHR) for a comminuted fracture of the proximal humerus. To increase the bone union rate, we reduce tuberosities to overlap on the humeral shaft by approx. 1 cm and fix them with cable wire. Herein, we retrospectively investigated the clinical and radiographic outcomes of our procedure. Twenty-six patients who underwent cementless HHR for the treatment of comminuted fractures of the proximal humerus were investigated. The Constant-Murley score, active shoulder mobility, and bone union rate were evaluated. The mean duration of follow-up was 56.3 months (range 24-197). At the final follow-up, the average Constant-Murley score was 58 (range 40-76). Forward elevation was 126° on average (range 35°-180°). Twenty-three cases (88%) showed bone union between the tuberosities and the shaft at an average follow-up of 4.1 months (range 4-5 months) after surgery. Non-union was noted in 1 case, and bone resorption was noted in 2 cases. The bone union rate and the clinical outcome of our procedure were relatively favorable en-copyright= kn-copyright= en-aut-name=HiranakaTakaaki en-aut-sei=Hiranaka en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KonishiikeTaizo en-aut-sei=Konishiike en-aut-mei=Taizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MikasaMotohiko en-aut-sei=Mikasa en-aut-mei=Motohiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Japanese Red Cross Okayama Hospital kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Density and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=cDepartment of Orthopaedic Surgery, Matsudo Orthopaedic Hospital kn-affil= en-keyword=humeral head replacement kn-keyword=humeral head replacement en-keyword=tuberosity-overlapping technique kn-keyword=tuberosity-overlapping technique en-keyword=bone union of tuberosities kn-keyword=bone union of tuberosities en-keyword=cable wire kn-keyword=cable wire en-keyword=cementless stem kn-keyword=cementless stem END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=4 article-no= start-page=611 end-page=633 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190320 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Method to Suppress Isobaric and Polyatomic Interferences for Measurements of Highly Siderophile Elements in Desilicified Geological Samples en-subtitle= kn-subtitle= en-abstract= kn-abstract= Sample decomposition using inverse aqua regia at elevated temperatures and pressures (e.g., Carius tube or high‐pressure asher) is the most common method used to extract highly siderophile elements (HSEs: Ru, Rh, Pd, Re, Os, Ir, Pt and Au) from geological samples. Recently, it has been recognised that additional HF desilicification is necessary to better recover HSEs, potentially contained within silicate or oxide minerals in mafic samples, which cannot be dissolved solely by inverse aqua regia. However, the abundance of interfering elements tends to increase in the eluent when conventional ion‐exchange purification procedures are applied to desilicified samples. In this study, we developed an improved purification method to determine HSEs in desilicified samples. This method enables the reduction of the ratios of isobaric and polyatomic interferences, relative to the measured intensities of HSE isotope masses, to less than a few hundred parts per million. Furthermore, the total procedural blanks are either comparable to or lower than conventional methods. Thus, this method allows accurate and precise HSE measurements in mafic and ultramafic geological samples, without the need for interference corrections. Moreover, the problem of increased interfering elements, such as Zr for Pd and Cr for Ru, is circumvented for the desilicified samples. en-copyright= kn-copyright= en-aut-name=ZhouXiaoyu en-aut-sei=Zhou en-aut-mei=Xiaoyu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamanakaMasahiro en-aut-sei=Yamanaka en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakaguchiChie en-aut-sei=Sakaguchi en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamuraEizo en-aut-sei=Nakamura en-aut-mei=Eizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=2 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=3 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=4 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=5 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= en-keyword=highly siderophile elements kn-keyword=highly siderophile elements en-keyword=desilicification kn-keyword=desilicification en-keyword=isotope dilution method kn-keyword=isotope dilution method en-keyword=high resolution ICP-MS kn-keyword=high resolution ICP-MS en-keyword=N-TIMS kn-keyword=N-TIMS END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=3 article-no= start-page=241 end-page=246 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Safety of Surgical Treatment for Elderly Patients with Gallbladder Carcinoma en-subtitle= kn-subtitle= en-abstract= kn-abstract= Gallbladder carcinoma (GBC) is a common malignancy with a poor prognosis. With the average life expectancy increasing globally, the incidence of GBC is predicted to increase as well. We investigated the safety and feasibility of surgical treatment for elderly patients with GBC. We retrospectively compared clinical pathological data and treatment outcomes in 45 consecutive GBC patients (23 patients ≥ 75 years [elderly group] and 22 patients < 75 years [younger group]) who underwent curative resection at the Iwakuni Center from January 2008 to December 2017. The proportion of preoperative comorbidities and anticoagulant use was significantly higher in the elderly group. The American Society of Anesthesiologists score was higher in the elderly versus the younger group, and the elderly group had significantly shorter operation times. Reduced activities of daily living was more common in the elderly versus younger group. The percentage of radical resection and overall 3-year survival (66.6% younger vs. 64.4% elderly) were similar between the groups. Controlling Nutritional Status (CONUT) score ≥ 3 and R0 resection were identified as prognostic factors for overall survival rate among all patients. After careful patient selection, en-copyright= kn-copyright= en-aut-name=UtsumiMasashi en-aut-sei=Utsumi en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AokiHideki en-aut-sei=Aoki en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishimuraSeitaro en-aut-sei=Nishimura en-aut-mei=Seitaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UneYuta en-aut-sei=Une en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KashimaHajime en-aut-sei=Kashima en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimuraYuji en-aut-sei=Kimura en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TaniguchiFumitaka en-aut-sei=Taniguchi en-aut-mei=Fumitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ArataTakashi en-aut-sei=Arata en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KatsudaKoh en-aut-sei=Katsuda en-aut-mei=Koh kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TanakayaKohji en-aut-sei=Tanakaya en-aut-mei=Kohji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=2 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=3 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=4 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=5 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center, kn-affil= affil-num=6 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=7 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=8 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=9 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= affil-num=10 en-affil=Department of Surgery, National Hospital Organization, Iwakuni Clinical Center kn-affil= en-keyword=elderly patient kn-keyword=elderly patient en-keyword=gallbladder carcinoma kn-keyword=gallbladder carcinoma en-keyword=prognostic factor kn-keyword=prognostic factor en-keyword=surgical treatment kn-keyword=surgical treatment END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=3 article-no= start-page=223 end-page=228 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Post-traumatic Articular Cartilage Lesions Increase at Second-look Arthroscopy Following Primary Anterior Cruciate Ligament Reconstruction en-subtitle= kn-subtitle= en-abstract= kn-abstract= Anterior cruciate ligament (ACL) reconstruction (ACLR) after ACL rupture improves the instability of the knee joint and decreases mechanical stress to the meniscus and articular cartilage. However, there are reports that post-traumatic osteoarthritis (PTOA) is observed over time following ACLR. In this study, we assessed changes in cartilage lesions by arthroscopic findings following anatomical double-bundle ACLR and at post-operative second-look arthroscopy about 14 months later. We retrospectively evaluated 37 knees in cases with patients <40 years of age who had undergone an anatomical double-bundle ACL reconstruction <1 year after ACL rupture injury from March 2012 to December 2016. Clinical results and arthroscopic cartilage/meniscal lesion were evaluated and compared between a cartilage lesion-detected group and intact-cartilage group. Surgery improved anteroposterior laxity and other clinical measures; however, cartilage lesions were detected at 11 sites during ACLR and at 54 sites at second-look arthroscopy. The periods from injury to second-look arthroscopy and from ACLR to second-look arthroscopy were significantly longer in the cartilage-lesion group (n=23) than in the intact-cartilage group (n=14). Conversely, 96% of meniscal damage observed during ACLR was cured at the time of second-look arthroscopy. Knee articular cartilage lesions after ACL rupture cannot be completely suppressed, even using the anatomical ACL reconstruction technique. This study suggested that articular cartilage lesions can progress to a level that can be confirmed arthroscopically at approximately 17 months after ACL injury. Therefore, in ACLR patients, the possibility of developing knee articular cartilage lesions and PTOA should be considered. en-copyright= kn-copyright= en-aut-name=SugiuKazuhisa en-aut-sei=Sugiu en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FurumatsuTakayuki en-aut-sei=Furumatsu en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KodamaYuya en-aut-sei=Kodama en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KamatsukiYusuke en-aut-sei=Kamatsuki en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkazakiYoshiki en-aut-sei=Okazaki en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OkazakiYuki en-aut-sei=Okazaki en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HiranakaTakaaki en-aut-sei=Hiranaka en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=anterior cruciate ligament reconstruction kn-keyword=anterior cruciate ligament reconstruction en-keyword=post-traumatic osteoarthritis kn-keyword=post-traumatic osteoarthritis en-keyword=meniscal lesion kn-keyword=meniscal lesion en-keyword=cartilage lesions kn-keyword=cartilage lesions en-keyword=second-look arthroscopy kn-keyword=second-look arthroscopy END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=3 article-no= start-page=197 end-page=203 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Geriatric Trauma in Patients ≧85 Years Old in an Urban District of Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract= Japan's population has been skewing toward the elderly, but the outcomes of advanced elderly trauma are not clear. Here we compared the outcomes of very elderly trauma patients (≧85 years old) with those of 65- to 84-year-old trauma patients. We retrospectively reviewed the medical records of patients treated at Hyogo Emergency Medical Center from August 2010 to August 2016; 631 patients were entered in the study. We divided them into the younger geriatrics (YG group, 65-84 years old: n=534) and older geriatrics (OG group, ≧85 years old: n=97). The group’s patient characteristics, mortality, 1-year survival rate, and Barthel index were tabulated and compared. The patients’ mean age was 75.6±7.5 years. There was no significant difference in mortality between the YG and OG groups (9.6% vs. 15.1%, odds ratio [OR] 1.73; 95% confidence interval [CI] 0.93-3.23, p=0.083). The 1-year survival rate (94.4% vs. 77.8%, OR 0.19, 95% CI 0.07-0.51; p<0.01) and Barthel index (Median score; 100 (IQR: 85-100) vs. 80 (IQR: 15-95), OR 0.98, 95% CI 0.97 to 0.99, p<0.01) differed significantly between the groups. Our study did not find a significant difference in-hospital mortality between patients in the YG group and those in the OG group. en-copyright= kn-copyright= en-aut-name=NishimuraTakeshi en-aut-sei=Nishimura en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaitoHiromichi en-aut-sei=Naito en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuyamaShigenari en-aut-sei=Matsuyama en-aut-mei=Shigenari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshiharaSatoshi en-aut-sei=Ishihara en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakaoAtsunori en-aut-sei=Nakao en-aut-mei=Atsunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NakayamaShinichi en-aut-sei=Nakayama en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Hyogo Emergency Medical Center kn-affil= affil-num=4 en-affil=Hyogo Emergency Medical Center kn-affil= affil-num=5 en-affil=Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Hyogo Emergency Medical Center kn-affil= en-keyword=aged kn-keyword=aged en-keyword=injury kn-keyword=injury en-keyword=mortality kn-keyword=mortality en-keyword=morbidity kn-keyword=morbidity en-keyword=trauma kn-keyword=trauma END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=3 article-no= start-page=189 end-page=195 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Anti-N-Methyl-D-Aspartate Receptor Encephalitis in Psychiatry en-subtitle= kn-subtitle= en-abstract= kn-abstract=Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a recently-discovered autoimmune disorder in which antibodies target NMDAR in the brain. The number of reported cases of anti-NMDAR encephalitis has increased rapidly. Anti-NMDAR encephalitis can be mistakenly diagnosed as psychiatric disorders because many patients present with prominent psychiatric symptoms and visit psychiatric institutions first. Thus, psychiatrists should cultivate a better understanding of anti-NMDAR encephalitis. In this review, we present the mechanisms, epidemiology, symptoms and clinical course, diagnostic tests, treatment and outcomes of patients with anti-NMDAR encephalitis. Furthermore, we discuss the diversity of clinical spectra of anti-NMDAR encephalitis, and demonstrate a differential diagnosis of psychiatric disease from the perspective of psychiatry. en-copyright= kn-copyright= en-aut-name=SakamotoShinji en-aut-sei=Sakamoto en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawaiHiroki en-aut-sei=Kawai en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkahisaYuko en-aut-sei=Okahisa en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TsutsuiKo en-aut-sei=Tsutsui en-aut-mei=Ko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanbayashiTakashi en-aut-sei=Kanbayashi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TanakaKeiko en-aut-sei=Tanaka en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MizukiYutaka en-aut-sei=Mizuki en-aut-mei=Yutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakakiManabu en-aut-sei=Takaki en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamadaNorihito en-aut-sei=Yamada en-aut-mei=Norihito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Neuropsychiatry, Akita University Graduate School of Medicine kn-affil= affil-num=5 en-affil=Department of Neuropsychiatry, Akita University Graduate School of Medicine kn-affil= affil-num=6 en-affil=Department of Animal Model Development, Brain Research Institute, Niigata University kn-affil= affil-num=7 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=NMDAR kn-keyword=NMDAR en-keyword=encephalitis kn-keyword=encephalitis en-keyword=psychiatric symptom kn-keyword=psychiatric symptom en-keyword=schizophrenia kn-keyword=schizophrenia en-keyword=mood disorder kn-keyword=mood disorder END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=2 article-no= start-page=127 end-page=133 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Thyroid Function Decline and Diet in Female High School Long-distance Runners en-subtitle= kn-subtitle= en-abstract= kn-abstract= We aimed to clarify the state of thyroid function in female high school long-distance runners. We evaluated the associations between thyroid function and menstrual condition, bone mineral density (BMD), nutritious status, and body composition. The subjects’ height and weight were measured, along with fat percentage, fat mass, muscle mass, and BMD with dual-energy X-ray absorptiometry. A nutrition and dietary survey measured the subjects’ intake of energy and nutrients based on meals provided at the subjects’ dorm for 3 days in July of 2016 and 2017. Blood parameters including thyroid hormone and estradiol were measured. Most of the subjects (81.3%) were underweight (body mass index <18.5). The thyroid hormone free T3 value was decreased, but TSH was not increased and was similar to that observed in individuals with anorexia nervosa. In our subjects, thyroid hormone was associated with BMD and nutritional intake. To improve the menstruation abnormality of female athletes and to increase their bone density, the athletes’ weight should be managed by proper nutrient intake and the maintenance of their thyroid function. en-copyright= kn-copyright= en-aut-name=IwasakiYukari en-aut-sei=Iwasaki en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyaharaKimiko en-aut-sei=Miyahara en-aut-mei=Kimiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MiyatakeNobuyuki en-aut-sei=Miyatake en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NakatsukaMikiya en-aut-sei=Nakatsuka en-aut-mei=Mikiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Health Sciences, Okayama University kn-affil= affil-num=2 en-affil=Faculty of Health Care, Department of Nutrition, Kiryu University kn-affil= affil-num=3 en-affil=Department of Hygiene, Faculty of Medicine, Kagawa University kn-affil= affil-num=4 en-affil=Graduate School of Health Sciences, Okayama University kn-affil= en-keyword=thyroid function kn-keyword=thyroid function en-keyword=nutritious status kn-keyword=nutritious status en-keyword=female high school long-distance runners kn-keyword=female high school long-distance runners en-keyword=bone mineral density kn-keyword=bone mineral density en-keyword=menstrual condition kn-keyword=menstrual condition END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1 article-no= start-page=77 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Flap Reconstruction for Esophageal Perforation Following Anterior Cervical Plate Fixation en-subtitle= kn-subtitle= en-abstract= kn-abstract= Anterior cervical plate fixation is a common surgical treatment for cervical spine trauma, disc herniation, or cervical spondylosis. Esophageal perforation following anterior cervical plate fixation is a rare but serious complication. Management of esophageal perforation is controversial; however, we suggest treating most cases surgically because this condition is slow to heal and often fatal. We managed 2 cases of esophageal perforation following anterior cervical plate fixation by flap reconstruction with the pectoralis major muscle in one case and a jejunal free flap in the other. Here, we report our experience and review the surgical indications. en-copyright= kn-copyright= en-aut-name=MoritaMio en-aut-sei=Morita en-aut-mei=Mio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsumotoHiroshi en-aut-sei=Matsumoto en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShirakawaYasuhiro en-aut-sei=Shirakawa en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NomaKazuhiro en-aut-sei=Noma en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanabeShunsuke en-aut-sei=Tanabe en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimataYoshihiro en-aut-sei=Kimata en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=2 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=4 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= affil-num=6 en-affil=Department of Plastic and Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science kn-affil= en-keyword=anterior cervical plate fixation kn-keyword=anterior cervical plate fixation en-keyword=esophageal perforation kn-keyword=esophageal perforation en-keyword=reconstruction kn-keyword=reconstruction en-keyword=pectoralis major flap kn-keyword=pectoralis major flap en-keyword=jejunal free flap kn-keyword=jejunal free flap END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1 article-no= start-page=29 end-page=39 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Histidine-rich Glycoprotein Could Be an Early Predictor of Vasospasm after Aneurysmal Subarachnoid Hemorrhage en-subtitle= kn-subtitle= en-abstract= kn-abstract= Cerebral vasospasm (CVS) is a major contributor to the high morbidity and mortality of aneurysmal subarachnoid hemorrhage (aSAH) patients. We measured histidine-rich glycoprotein (HRG), a new biomarker of aSAH, in cerebrospinal fluid (CSF) to investigate whether HRG might be an early predictor of CVS. A total of seven controls and 14 aSAH patients (8 males, 6 females aged 53.4±15.4 years) were enrolled, and serial CSF and serum samples were taken. We allocated these samples to three phases (T1-T3) and measured HRG, interleukin (IL)-6, fibrinopeptide A (FpA), and 8-hydroxy-2’-deoxyguanosine (8OHdG) in the CSF, and the HRG in serum. We also examined the release of HRG in rat blood incubated in artificial CSF. In contrast to the other biomarkers examined, the change in the CSF HRG concentration was significantly different between the nonspasm and spasm groups (p<0.01). The rat blood/CSF model revealed a time course similar to that of the human CSF samples in the non-spasm group. HRG thus appears to have the potential to become an early predictor of CVS. In addition, the interaction of HRG with IL-6, FpA, and 8OHdG may form the pathology of CVS. en-copyright= kn-copyright= en-aut-name=MatsumotoAtsushi en-aut-sei=Matsumoto en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraTakehiro en-aut-sei=Nakamura en-aut-mei=Takehiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShinomiyaAya en-aut-sei=Shinomiya en-aut-mei=Aya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawakitaKenya en-aut-sei=Kawakita en-aut-mei=Kenya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawanishiMasahiko en-aut-sei=Kawanishi en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MiyakeKeisuke en-aut-sei=Miyake en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KurodaYasuhiro en-aut-sei=Kuroda en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KeepRichard F. en-aut-sei=Keep en-aut-mei=Richard F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TamiyaTakashi en-aut-sei=Tamiya en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Department of Neurological Surgery, Kagawa University Faculty of Medicine kn-affil= affil-num=2 en-affil=Department of Medical Technology, Kagawa Prefectural University of Health Sciences kn-affil= affil-num=3 en-affil=Department of Neurological Surgery, Kagawa University Faculty of Medicine kn-affil= affil-num=4 en-affil=Emergency Medical Center, Kagawa University Hospital kn-affil= affil-num=5 en-affil=Department of Neurological Surgery, Kagawa University Faculty of Medicine kn-affil= affil-num=6 en-affil=Department of Neurological Surgery, Kagawa University Faculty of Medicine kn-affil= affil-num=7 en-affil=Emergency Medical Center, Kagawa University Hospital kn-affil= affil-num=8 en-affil=Department of Neurosurgery, University of Michigan kn-affil= affil-num=9 en-affil=Department of Neurological Surgery, Kagawa University Faculty of Medicine kn-affil= en-keyword=biomarker kn-keyword=biomarker en-keyword=histidine-rich glycoprotein kn-keyword=histidine-rich glycoprotein en-keyword=predictor kn-keyword=predictor en-keyword=subarachnoid hemorrhage kn-keyword=subarachnoid hemorrhage en-keyword=vasospasm kn-keyword=vasospasm END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Role of Kallikrein-Related Peptidases in Atopic Dermatitis en-subtitle= kn-subtitle= en-abstract= kn-abstract= Excessive protease activity is a characteristic abnormality that affects the epidermal barrier in patients with atopic dermatitis (AD). Kallikrein-related peptidases (KLKs) are excessively expressed in AD lesions, and it is suggested that the abnormal action of KLKs is involved in the skin barrier dysfunction in AD. In other words, overexpressed KLKs disrupt the normal barrier function, and due to that breakdown, external substances that can become antigens of AD easily invade the epidermis, resulting in dermatitis, coupled with the induction of Th2 cytokines. Further investigations are required to elucidate the role of KLKs in AD; this knowledge could contribute to the design of new therapeutic and prophylactic drugs for AD. en-copyright= kn-copyright= en-aut-name=MorizaneShin en-aut-sei=Morizane en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=atopic dermatitis kn-keyword=atopic dermatitis en-keyword=kallikrein-related peptidases kn-keyword=kallikrein-related peptidases en-keyword=epidermal barrier dysfunction kn-keyword=epidermal barrier dysfunction END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=6 article-no= start-page=553 end-page=562 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=201812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Changes in Serum Biochemical Markers in Relation to Chief Complaints and Aging in General Medicine en-subtitle= kn-subtitle= en-abstract= kn-abstract= To clarify potential relationships between chief complaints of patients and laboratory data with a focus on aging-related changes, we retrospectively analyzed the data of 843 patients who visited a general medicine department for the first time. Their chief complaints were classified into 8 major symptoms: visceral pain, somatic pain, fever, cough, dizziness, fatigue, appetite loss, and edema. We compared the laboratory data obtained from the patients with complaints with the data of symptom-free (control) patients. The serum sodium and potassium levels in the fever group were decreased compared to those in the control group. In the fever group, the serum sodium level was inversely correlated with age. The ratio of serum urea nitrogen to creatinine (UN/Cr) was increased in the appetite-loss group. There were significant age-dependent increases in the UN/Cr ratio in the appetite-loss and edema groups. Of note, serum levels of free thyroxin were lower in the dizziness group compared to the control group. In addition, the free thyroxin level was inversely correlated with age in the dizziness group but not in the asymptomatic control group. Collectively, the results indicated that osmolality-related laboratory data are strongly associated with individual primary symptoms at the first visit regardless of the final diagnosis. The consideration of age-dependent changes of these markers is helpful for diagnosing latent disorders based on various primary symptoms. en-copyright= kn-copyright= en-aut-name=OmuraDaisuke en-aut-sei=Omura en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatoAsuka en-aut-sei=Sato en-aut-mei=Asuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkaKosuke en-aut-sei=Oka en-aut-mei=Kosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HanayamaYoshihisa en-aut-sei=Hanayama en-aut-mei=Yoshihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OgawaHiroko en-aut-sei=Ogawa en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=ObikaMikako en-aut-sei=Obika en-aut-mei=Mikako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OtsukaFumio en-aut-sei=Otsuka en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of General Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=dizziness kn-keyword=dizziness en-keyword=fever kn-keyword=fever en-keyword=osmolality kn-keyword=osmolality en-keyword=serum sodium kn-keyword=serum sodium en-keyword=thyroid dysfunction kn-keyword=thyroid dysfunction END start-ver=1.4 cd-journal=joma no-vol=81 cd-vols= no-issue=6 article-no= start-page=063623 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100614 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Spin textures in condensates with large dipole moments en-subtitle= kn-subtitle= en-abstract= kn-abstract= We have solved numerically the ground states of a Bose-Einstein condensate in the presence of dipolar interparticle forces using a semiclassical approach. Our motivation is to model, in particular, the spontaneous spin textures emerging in quantum gases with large dipole moments, such as Cr-52 or Dy condensates, or ultracold gases consisting of polar molecules. For a pancake-shaped harmonic ( optical) potential, we present the ground-state phase diagram spanned by the strength of the nonlinear coupling and dipolar interactions. In an elongated harmonic potential, we observe a helical spin texture. The textures calculated according to the semiclassical model in the absence of external polarizing fields are predominantly analogous to previously reported results for a ferromagnetic F = 1 spinor Bose-Einstein condensate, suggesting that the spin textures arising from the dipolar forces are largely independent of the value of the quantum number F or the origin of the dipolar interactions. en-copyright= kn-copyright= en-aut-name=HuhtamakiJ. A. M. en-aut-sei=Huhtamaki en-aut-mei=J. A. M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiM. en-aut-sei=Takahashi en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SimulaT. P. en-aut-sei=Simula en-aut-mei=T. P. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MizushimaT. en-aut-sei=Mizushima en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MachidaK. en-aut-sei=Machida en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Physics, Okayama University kn-affil= affil-num=2 en-affil=Department of Physics, Okayama University kn-affil= affil-num=3 en-affil=Department of Physics, Okayama University kn-affil= affil-num=4 en-affil=Department of Physics, Okayama University kn-affil= affil-num=5 en-affil=Department of Physics, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=2 article-no= start-page=127 end-page=133 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=201704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Outcome of Radiation Monotherapy for High-risk Patients with Stage I Esophageal Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=Currently, chemoradiation is the most widely used nonsurgical treatment for esophageal cancer. However, some patients, particularly the very elderly or those with severe vital organ dysfunction, face difficulty with the chemotherapy component. We therefore examined the outcome of radiation therapy (RT) alone for patients with esophageal cancer at our facility. Between January 2005 and December 2014, 84 patients underwent RT at our hospital, and 78 of these patients received concomitant chemotherapy. The remaining 6 patients underwent RT alone; these patients were considered to be high-risk and to have no lymph node metastasis (stage I). Five of them received irradiation up to a curative dose: 4 showed a complete response (CR) and 1 showed a partial response (PR). Of the patients exhibiting CR, 3 are currently living recurrence-free, whereas 1 patient underwent endoscopic submucosal dissection (ESD) as salvage therapy for local recurrence, with no subsequent recurrence. High-risk stage I esophageal cancer patients can be treated radically with RT alone under certain conditions. In the future, to broaden the indications for RT monotherapy to include some degree of advanced cancers, a novel concurrent therapy should be identified. en-copyright= kn-copyright= en-aut-name=ShirakawaYasuhiro en-aut-sei=Shirakawa en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NomaKazuhiro en-aut-sei=Noma en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaedaNaoaki en-aut-sei=Maeda en-aut-mei=Naoaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanabeShunsuke en-aut-sei=Tanabe en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KurodaShinji en-aut-sei=Kuroda en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KagawaShunsuke en-aut-sei=Kagawa en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KatsuiKuniaki en-aut-sei=Katsui en-aut-mei=Kuniaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KatayamaNorihisa en-aut-sei=Katayama en-aut-mei=Norihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KanazawaSusumu en-aut-sei=Kanazawa en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine,Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Proton Beam Therapy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=esophageal cancer kn-keyword=esophageal cancer en-keyword=radiation therapy kn-keyword=radiation therapy en-keyword=high-risk patient kn-keyword=high-risk patient END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=6 article-no= start-page=435 end-page=439 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Urinary Cross-linked N-terminal Telopeptide of Type I Collagen Levels of Infants with Osteogenesis Imperfecta and Healthy Infants en-subtitle= kn-subtitle= en-abstract= kn-abstract=The urinary cross-linked N-terminal telopeptide of type I collagen (uNTx) levels in infantile osteogenesis imperfecta (OI) have not been well studied. Here we investigated the levels of uNTx in infants with OI and healthy infants. We collected spot urine samples from 30 infants with OI (male/female, 14/16; Sillence classification, I/II/III/IV: 15/3/6/6; age, 5.2±4.4 months) and 120 healthy infants (male/female, 75/45; age, 5.1±4.1 months) for the measurement of uNTx levels. The uNTx levels of the OI infants were significantly lower than those of the healthy infants (mean±SD, 1,363.7±530.1 vs. 2,622.2±1,202.6 nmol BCE/mmol Cr; p＜0.001). The uNTx levels of the infants with type I OI were significantly lower than those of the age-matched healthy infants, although an overlap was observed between the 2 groups. Among the 1-month-old infants, the uNTx levels of the infants with types I, III or IV OI were significantly lower than those of the healthy infants, without overlap (1,622.5±235.8 vs. 3,781.0±1,027.1 nmol BCE/mmol Cr; p＜0.001). These results indicate that uNTx levels are significantly lower in infants with OI than in healthy infants, and they suggest that uNTx might be useful as a reference for diagnosing OI. en-copyright= kn-copyright= en-aut-name=YamashitaMiho en-aut-sei=Yamashita en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HasegawaKosei en-aut-sei=Hasegawa en-aut-mei=Kosei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HiguchiYousuke en-aut-sei=Higuchi en-aut-mei=Yousuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MiyaiTakayuki en-aut-sei=Miyai en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkadaAyumi en-aut-sei=Okada en-aut-mei=Ayumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TanakaHiroyuki en-aut-sei=Tanaka en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TsukaharaHirokazu en-aut-sei=Tsukahara en-aut-mei=Hirokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Pediatrics, Okayama University Hospital kn-affil= affil-num=5 en-affil=Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Pediatrics, Okayama Saiseikai General Hospital kn-affil= affil-num=7 en-affil=Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=bone resorption marker kn-keyword=bone resorption marker en-keyword=bone turnover kn-keyword=bone turnover en-keyword=bone mass kn-keyword=bone mass END start-ver=1.4 cd-journal=joma no-vol=128 cd-vols= no-issue=3 article-no= start-page=201 end-page=205 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20161201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ectopic sebaceous glands in the esophagus that became evident over a three-year span kn-title=3 年間の内視鏡所見の変化を観察できた食道異所性皮脂腺の1 例 en-subtitle= kn-subtitle= en-abstract= kn-abstract= A 43-year-old Japanese woman was diagnosed with ectopic sebaceous glands in the esophagus by esophagogastroduodenoscopy and biopsy. At the age of 46, typical ectopic sebaceous glands were recognized in the upper esophagus, whereas yellowish white granules were faintly observed in the lower esophagus. Esophagogastroduodenoscopy examinations were repeated when she was 47 and again at 50 years old, and the lesions in the lower esophagus had become more evident over the ensuing 3 years. Esophageal ectopic sebaceous glands are relatively infrequent, and there have been few case reports describing the progression of the endoscopic features. We also report the clinical and endoscopic features of the five similar cases with pathologically proven ectopic sebaceous glands in the esophagus. en-copyright= kn-copyright= en-aut-name=IwamuroMasaya en-aut-sei=Iwamuro en-aut-mei=Masaya kn-aut-name=岩室雅也 kn-aut-sei=岩室 kn-aut-mei=雅也 aut-affil-num=1 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name=岡田裕之 kn-aut-sei=岡田 kn-aut-mei=裕之 aut-affil-num=2 ORCID= en-aut-name=HaradaKeita en-aut-sei=Harada en-aut-mei=Keita kn-aut-name=原田馨太 kn-aut-sei=原田 kn-aut-mei=馨太 aut-affil-num=3 ORCID= en-aut-name=KanzakiHiromitsu en-aut-sei=Kanzaki en-aut-mei=Hiromitsu kn-aut-name=神崎洋光 kn-aut-sei=神崎 kn-aut-mei=洋光 aut-affil-num=4 ORCID= en-aut-name=HoriKeisuke en-aut-sei=Hori en-aut-mei=Keisuke kn-aut-name=堀圭介 kn-aut-sei=堀 kn-aut-mei=圭介 aut-affil-num=5 ORCID= en-aut-name=KitaMasahide en-aut-sei=Kita en-aut-mei=Masahide kn-aut-name=喜多雅英 kn-aut-sei=喜多 kn-aut-mei=雅英 aut-affil-num=6 ORCID= en-aut-name=KawanoSeiji en-aut-sei=Kawano en-aut-mei=Seiji kn-aut-name=川野誠司 kn-aut-sei=川野 kn-aut-mei=誠司 aut-affil-num=7 ORCID= en-aut-name=KawaharaYoshiro en-aut-sei=Kawahara en-aut-mei=Yoshiro kn-aut-name=河原祥朗 kn-aut-sei=河原 kn-aut-mei=祥朗 aut-affil-num=8 ORCID= en-aut-name=TanakaTakehiro en-aut-sei=Tanaka en-aut-mei=Takehiro kn-aut-name=田中健大 kn-aut-sei=田中 kn-aut-mei=健大 aut-affil-num=9 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name=山本和秀 kn-aut-sei=山本 kn-aut-mei=和秀 aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 affil-num=2 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 affil-num=3 en-affil=Department of Endoscopy, Okayama University Hospital kn-affil=岡山大学病院　光学医療診療部 affil-num=4 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 affil-num=5 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 affil-num=6 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 affil-num=7 en-affil=Department of Endoscopy, Okayama University Hospital kn-affil=岡山大学病院　光学医療診療部 affil-num=8 en-affil=Department of Endoscopy, Okayama University Hospital kn-affil=岡山大学病院　光学医療診療部 affil-num=9 en-affil=Department of Pathology, Okayama University Hospital kn-affil=岡山大学病院　病理診断科 affil-num=10 en-affil=Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・肝臓内科学 en-keyword=食道異所性脂腺（ectopic sebaceous glands in the esophagus） kn-keyword=食道異所性脂腺（ectopic sebaceous glands in the esophagus） en-keyword=粘膜下腫瘍（submucosal tumor） kn-keyword=粘膜下腫瘍（submucosal tumor） en-keyword=食道黄色腫（esophageal xanthoma） kn-keyword=食道黄色腫（esophageal xanthoma） END start-ver=1.4 cd-journal=joma no-vol=128 cd-vols= no-issue=2 article-no= start-page=103 end-page=109 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Identification of the adipokine ‘vaspin’ and its significance in metabolic syndrome kn-title=アディポカイン「バスピン」の同定とメタボリックシンドロームにおける意義 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name=和田淳 kn-aut-sei=和田 kn-aut-mei=淳 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学 en-keyword=metabolic syndrome kn-keyword=metabolic syndrome en-keyword=adipokine kn-keyword=adipokine en-keyword=atherosclerosis kn-keyword=atherosclerosis en-keyword=endothelial cells kn-keyword=endothelial cells en-keyword=apoptosis kn-keyword=apoptosis END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=3 article-no= start-page=187 end-page=195 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20151201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Basic and clinical research regarding vascular endothelial function kn-title=血管内皮機能を対象にした基礎および臨床医学研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsukaharaHirokazu en-aut-sei=Tsukahara en-aut-mei=Hirokazu kn-aut-name=塚原宏一 kn-aut-sei=塚原 kn-aut-mei=宏一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 en-keyword=アルギニン代謝 kn-keyword=アルギニン代謝 en-keyword=一酸化窒素 kn-keyword=一酸化窒素 en-keyword=ガス生物学 kn-keyword=ガス生物学 en-keyword=血管内皮学 kn-keyword=血管内皮学 en-keyword=酸化ストレス kn-keyword=酸化ストレス END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=2 article-no= start-page=103 end-page=109 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150803 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Role of COLXV/XVⅢ gene, Multiplexin, as a basement membrane toolkit kn-title=基底膜ツールキットとしてのXV/XVⅢ型コラーゲン遺伝子の機能 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OohashiToshitaka en-aut-sei=Oohashi en-aut-mei=Toshitaka kn-aut-name=大橋俊孝 kn-aut-sei=大橋 kn-aut-mei=俊孝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 en-keyword=基底膜 kn-keyword=基底膜 en-keyword=ツールキット kn-keyword=ツールキット en-keyword=XV/XVⅢ型コラーゲン kn-keyword=XV/XVⅢ型コラーゲン en-keyword=プロテオグリカン kn-keyword=プロテオグリカン en-keyword=ミトコンドリア kn-keyword=ミトコンドリア END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=1 article-no= start-page=63 end-page=65 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=PD-1,CTLA-4 kn-title=免疫疲弊分子PD-1，CTLA-4 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=EikawaShingo en-aut-sei=Eikawa en-aut-mei=Shingo kn-aut-name=榮川伸吾 kn-aut-sei=榮川 kn-aut-mei=伸吾 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　免疫学 END start-ver=1.4 cd-journal=joma no-vol=110 cd-vols= no-issue= article-no= start-page=29 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990315 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Proton Magnetic Resonance Spectroscopy in Patients with Migration Disorders kn-title=磁気共鳴スペクトロスコピー法による脳障害の評価 ―神経遊走障害の検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Proton Magnetic Resonance Spectroscopy (1H-MRS) can be used to detect cerebral metabolites including N-acetylaspartate (NAA)，creatine (Cr) and choline (Ch). Hence，clinical applications of this method for neuropediatric diseases can be expected. However，regarding neuronal migration disorders，there have been only a few reported studies. We therefore examined the lH-MRS in six patients with migration disorders，ages ranged from 8months to 28years 10months with a mean of 10years 10months. Investigation was performed using Magnetom H15 (Siemens) with a repetition time of 1500 msec and an echo time of 270msec. The ratio of NAA/Cr，Ch/Cr were examined. The volume of interest with the size of 2 × 2 × 2 ～ 3 × 3 × 5cm3 was chosen in the area including lesions，and a contralateral area without lesions was also investigated. Results were as follows. 1) The ratio of NAA/Cr was low in the area with lesions in all 6cases; 1.41，1.95，2.27 and 1.71 in cases with heterotopic gray matter，0.99 in one case with polymicrogyria，an d 1.30 in one case with hemimegalencephaly，contrasted with a contralataral area without lesions: 1.89， 2.89，2.87，2.55，3.26，2.03，respectively. 2) The ratio of Ch/Cr showed no consistent difference between the area including lesions and contralataral area without lesions. Our findings of a decreased NAA/Cr ratio can be inferred to reflect the decreased numbers of neuronal cell population，or reduced metabolism in the lesions. en-copyright= kn-copyright= en-aut-name=SanadaSatoshi en-aut-sei=Sanada en-aut-mei=Satoshi kn-aut-name=眞田敏 kn-aut-sei=眞田 kn-aut-mei=敏 aut-affil-num=1 ORCID= en-aut-name=AsanoTakashi en-aut-sei=Asano en-aut-mei=Takashi kn-aut-name=浅野孝 kn-aut-sei=浅野 kn-aut-mei=孝 aut-affil-num=2 ORCID= en-aut-name=OkaEiji en-aut-sei=Oka en-aut-mei=Eiji kn-aut-name=岡鍈次 kn-aut-sei=岡 kn-aut-mei=鍈次 aut-affil-num=3 ORCID= en-aut-name=KawaharaMichiko en-aut-sei=Kawahara en-aut-mei=Michiko kn-aut-name=河原道子 kn-aut-sei=河原 kn-aut-mei=道子 aut-affil-num=4 ORCID= en-aut-name=SakaeKatsumi en-aut-sei=Sakae en-aut-mei=Katsumi kn-aut-name=栄勝美 kn-aut-sei=栄 kn-aut-mei=勝美 aut-affil-num=5 ORCID= en-aut-name=HirakiNorio en-aut-sei=Hiraki en-aut-mei=Norio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=6 ORCID= en-aut-name=OhtaharaShunsuke en-aut-sei=Ohtahara en-aut-mei=Shunsuke kn-aut-name=大田原俊輔 kn-aut-sei=大田原 kn-aut-mei=俊輔 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学教育学部障害児教育講座 affil-num=2 en-affil= kn-affil=Department of Child Neurology，Okayama University Medical School affil-num=3 en-affil= kn-affil=Department of Child Neurology，Okayama University Medical School affil-num=4 en-affil= kn-affil=Department of Radiology，Okayama University Medical School affil-num=5 en-affil= kn-affil=Department of Radiology，Okayama University Medical School affil-num=6 en-affil= kn-affil=Department of Radiology，Okayama University Medical School affil-num=7 en-affil= kn-affil=Kibi International University Health Sciences School en-keyword=MR spectroscopy kn-keyword=MR spectroscopy en-keyword=migration disorder kn-keyword=migration disorder en-keyword=heterotopic gray matter kn-keyword=heterotopic gray matter en-keyword=polymicrogyria kn-keyword=polymicrogyria en-keyword=hemimegalencephaly kn-keyword=hemimegalencephaly END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=3 article-no= start-page=259 end-page=262 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20131202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Drug interaction (28. combination with therapeutic drugs used to treat overactive bladder) kn-title=薬物相互作用 (28―過活動膀胱治療薬の薬物相互作用） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HarutaYusuke en-aut-sei=Haruta en-aut-mei=Yusuke kn-aut-name=晴田佑介 kn-aut-sei=晴田 kn-aut-mei=佑介 aut-affil-num=1 ORCID= en-aut-name=KawasakiYoichi en-aut-sei=Kawasaki en-aut-mei=Yoichi kn-aut-name=河崎陽一 kn-aut-sei=河崎 kn-aut-mei=陽一 aut-affil-num=2 ORCID= en-aut-name=KitamuraYoshihisa en-aut-sei=Kitamura en-aut-mei=Yoshihisa kn-aut-name=北村佳久 kn-aut-sei=北村 kn-aut-mei=佳久 aut-affil-num=3 ORCID= en-aut-name=SendoToshiaki en-aut-sei=Sendo en-aut-mei=Toshiaki kn-aut-name=千堂年昭 kn-aut-sei=千堂 kn-aut-mei=年昭 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=2 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=3 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=4 en-affil= kn-affil=岡山大学病院　薬剤部 END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=3 article-no= start-page=235 end-page=238 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20131202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Two operated cases of mesenteric abscess caused by penetration of the transverse colon associated with a diverticulum kn-title=横行結腸憩室穿通による腸間膜膿瘍に対して手術を施行した２症例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We encountered 2 cases of penetration of fecal matter through a diverticulum of the transverse colon, which is a rare disease. Case 1 was a 33-year-old man who was examined in the clinic with a complaint of epigastralgia. Tenderness and muscular defense were found in the upper abdomen. On abdominal CT examination, wall thickening and increased fat concentration were seen in the transverse colon. He was diagnosed with peritonitis and underwent emergency surgery. On laparotomy, a tumor mass was found in the transverse colon close to the liver curvature. The patient was diagnosed with mesenteric abscess due to penetration of fecal matter through a diverticulum of the transverse colon. Right hemicolectomy was carried out. Case 2 was a 43-year-old woman who was examined in the clinic with a complaint of right lower abdominal pain. Muscular defense and rebound tenderness were found in the lower abdomen. On abdominal CT examination, abscess formation was seen in the right lower abdomen. She was diagnosed with peritonitis and underwent emergency surgery. On laparotomy, a tumor mass was found in the transverse colon. The patient was diagnosed with mesenteric abscess due to penetration of the transverse colon associated with a diverticulum. Partial removal of the transverse colon was carried out. en-copyright= kn-copyright= en-aut-name=MaedaNaoaki en-aut-sei=Maeda en-aut-mei=Naoaki kn-aut-name=前田直見 kn-aut-sei=前田 kn-aut-mei=直見 aut-affil-num=1 ORCID= en-aut-name=KondoHidenori en-aut-sei=Kondo en-aut-mei=Hidenori kn-aut-name=近藤秀則 kn-aut-sei=近藤 kn-aut-mei=秀則 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=医療法人敬和会近藤病院　外科 affil-num=2 en-affil= kn-affil=医療法人敬和会近藤病院　外科 en-keyword=横行結腸憩室（transverse colon diverticulum） kn-keyword=横行結腸憩室（transverse colon diverticulum） en-keyword=腸間膜内穿通（mesenteric penetration） kn-keyword=腸間膜内穿通（mesenteric penetration） en-keyword=腸間膜膿瘍（mesenteric abscess） kn-keyword=腸間膜膿瘍（mesenteric abscess） END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=3 article-no= start-page=205 end-page=209 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20131202 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of GBR12909 on affective behavior：Distinguishing motivational behavior from antidepressant-like and addiction-like behavior using the runway model of intracranial self-stimulation kn-title=GBR12909の情動行動への影響：脳内自己刺激行動の Runway法を用いた動機付け行動と，抗うつ様行動および依存様行動の区別 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=EsumiSatoru en-aut-sei=Esumi en-aut-mei=Satoru kn-aut-name=江角悟 kn-aut-sei=江角 kn-aut-mei=悟 aut-affil-num=1 ORCID= en-aut-name=SagaraHidenori en-aut-sei=Sagara en-aut-mei=Hidenori kn-aut-name=相良英憲 kn-aut-sei=相良 kn-aut-mei=英憲 aut-affil-num=2 ORCID= en-aut-name=NakamotoAkihiko en-aut-sei=Nakamoto en-aut-mei=Akihiko kn-aut-name=中本秋彦 kn-aut-sei=中本 kn-aut-mei=秋彦 aut-affil-num=3 ORCID= en-aut-name=KawasakiYoichi en-aut-sei=Kawasaki en-aut-mei=Yoichi kn-aut-name=河崎陽一 kn-aut-sei=河崎 kn-aut-mei=陽一 aut-affil-num=4 ORCID= en-aut-name=GomitaYutaka en-aut-sei=Gomita en-aut-mei=Yutaka kn-aut-name=五味田裕 kn-aut-sei=五味田 kn-aut-mei=裕 aut-affil-num=5 ORCID= en-aut-name=SendoToshiaki en-aut-sei=Sendo en-aut-mei=Toshiaki kn-aut-name=千堂年昭 kn-aut-sei=千堂 kn-aut-mei=年昭 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=2 en-affil= kn-affil=松山大学薬学部　医薬情報解析学 affil-num=3 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=4 en-affil= kn-affil=岡山大学病院　薬剤部 affil-num=5 en-affil= kn-affil=就実大学　薬学部 affil-num=6 en-affil= kn-affil=岡山大学病院　薬剤部 en-keyword=intracranial self-stimulation kn-keyword=intracranial self-stimulation en-keyword=GBR12909 kn-keyword=GBR12909 en-keyword=motivation kn-keyword=motivation en-keyword=depression kn-keyword=depression en-keyword=addiction kn-keyword=addiction END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=2 article-no= start-page=139 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of strangulated ileus caused by internal hernia through a defect in the broad ligament of the uterus kn-title=子宮広間膜裂孔ヘルニアによる絞扼性イレウスの１例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 41-year-old woman was admitted to our hospital because of lower abdominal pain. She had no history of laparotomy. An abdominal CT scan at the time of admission indicated closed-loop intestinal obstruction of the small intestine within the pelvis and deviation of the uterus to the right. We diagnosed this as a strangulated internal hernia and decided to conduct an emergency operation. The ileum had herniated through a defect in the broad ligament of the uterus. The strangulated intestinal loop, which was about 25cm long, was released, and the defect of the broad ligament was closed. The postoperative course was uneventful. The differential diagnosis of intestinal obstruction should include internal hernia, especially in the absence of a previous laparotomy. An abdominal CT scan is quite useful for the preoperative and prompt diagnosis of internal hernia through a defect in the broad ligament of the uterus. en-copyright= kn-copyright= en-aut-name=HaisaMinoru en-aut-sei=Haisa en-aut-mei=Minoru kn-aut-name=羽井佐実 kn-aut-sei=羽井佐 kn-aut-mei=実 aut-affil-num=1 ORCID= en-aut-name=SakaiRyo en-aut-sei=Sakai en-aut-mei=Ryo kn-aut-name=酒井亮 kn-aut-sei=酒井 kn-aut-mei=亮 aut-affil-num=2 ORCID= en-aut-name=KuriharaEisuke en-aut-sei=Kurihara en-aut-mei=Eisuke kn-aut-name=栗原英祐 kn-aut-sei=栗原 kn-aut-mei=英祐 aut-affil-num=3 ORCID= en-aut-name=TeramotoAtsushi en-aut-sei=Teramoto en-aut-mei=Atsushi kn-aut-name=寺本淳 kn-aut-sei=寺本 kn-aut-mei=淳 aut-affil-num=4 ORCID= en-aut-name=ShougaKazuhiko en-aut-sei=Shouga en-aut-mei=Kazuhiko kn-aut-name=庄賀一彦 kn-aut-sei=庄賀 kn-aut-mei=一彦 aut-affil-num=5 ORCID= en-aut-name=MitsuokaShintarou en-aut-sei=Mitsuoka en-aut-mei=Shintarou kn-aut-name=光岡晋太郎 kn-aut-sei=光岡 kn-aut-mei=晋太郎 aut-affil-num=6 ORCID= en-aut-name=NishimuraMamoru en-aut-sei=Nishimura en-aut-mei=Mamoru kn-aut-name=西村守 kn-aut-sei=西村 kn-aut-mei=守 aut-affil-num=7 ORCID= en-aut-name=SatouYuuki en-aut-sei=Satou en-aut-mei=Yuuki kn-aut-name=佐藤雄紀 kn-aut-sei=佐藤 kn-aut-mei=雄紀 aut-affil-num=8 ORCID= en-aut-name=NanbaJiro en-aut-sei=Nanba en-aut-mei=Jiro kn-aut-name=難波次郎 kn-aut-sei=難波 kn-aut-mei=次郎 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=2 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=3 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=4 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=5 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=6 en-affil= kn-affil=岡山市立市民病院 消化器外科 affil-num=7 en-affil= kn-affil=岡山市立市民病院 消化器内科 affil-num=8 en-affil= kn-affil=岡山市立市民病院 消化器内科 affil-num=9 en-affil= kn-affil=岡山市立市民病院 消化器内科 en-keyword=子宮広間膜裂孔（broad ligament） kn-keyword=子宮広間膜裂孔（broad ligament） en-keyword=内ヘルニア（internal hernia） kn-keyword=内ヘルニア（internal hernia） en-keyword=絞扼性イレウス（strangulated ileus） kn-keyword=絞扼性イレウス（strangulated ileus） en-keyword=MDCT（multi-detector CT） kn-keyword=MDCT（multi-detector CT） END start-ver=1.4 cd-journal=joma no-vol=18 cd-vols= no-issue=1 article-no= start-page=110 end-page=115 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201302 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Could salvage surgery after chemotherapy have clinical impact on cancer survival of patients with metastatic urothelial carcinoma? en-subtitle= kn-subtitle= en-abstract= kn-abstract=The clinical impact of salvage surgery after chemotherapy on cancer survival of patients with metastatic urothelial carcinoma is controversial. We aimed to verify the clinical role of salvage surgery by analyzing the long-term outcome in patients with urothelial carcinoma treated by chemotherapy. Between 2003 and 2010 at a single institution, 31 of 47 patients (66%) with metastatic urothelial carcinoma showed objective responses (CR in 4, PR in 27) after multiple courses of cisplatin/gemcitabine/paclitaxel-based chemotherapy, and a cohort of patients with partial response (PR) were retrospectively enrolled. Twelve (10 male and 2 female, median age 64.0 years) of 27 patients with PR underwent salvage surgeries after the chemotherapy: metastatectomy of residual lesions (10 retroperitoneal lymph nodes, 2 lung), and 6 radical surgeries for primary lesions as well. Progression-free survival and overall patient survival rates were analyzed retrospectively and compared with those of patients without salvage surgery. All 12 patients achieved surgical CR. Pathological findings of metastatic lesions showed viable cancer cells in 3 patients. In univariate analysis, sole salvage surgery affected overall survival in 27 patients with PR to the chemotherapy (P = 0.0037). Progression-free survival and overall survival rates in patients with salvage surgery were better than those in 15 PR patients without the surgery (39.8 vs. 0%, and 71.6 vs. 12.1% at 3 years, P = 0.01032 and 0.01048; log-rank test). Salvage surgery for patients with residual tumor who achieve partial response to chemotherapy could have a possible impact on cancer survival. en-copyright= kn-copyright= en-aut-name=BekkuKensuke en-aut-sei=Bekku en-aut-mei=Kensuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SaikaTakashi en-aut-sei=Saika en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KobayashiYasuyuki en-aut-sei=Kobayashi en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KioshimotoRyo en-aut-sei=Kioshimoto en-aut-mei=Ryo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanbaraTaiki en-aut-sei=Kanbara en-aut-mei=Taiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NasuYasutomo en-aut-sei=Nasu en-aut-mei=Yasutomo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KumonHiromi en-aut-sei=Kumon en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=2 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=3 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=4 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=5 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=6 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci affil-num=7 en-affil= kn-affil=Okayama Univ, Dept Urol, Grad Sch Med Dent & Pharmaceut Sci en-keyword=Chemotherapy kn-keyword=Chemotherapy en-keyword=Metastatectomy kn-keyword=Metastatectomy en-keyword=Metastatic urothelial carcinoma kn-keyword=Metastatic urothelial carcinoma en-keyword=Salvage surgery kn-keyword=Salvage surgery en-keyword=Urothelial carcinoma kn-keyword=Urothelial carcinoma END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=1 article-no= start-page=51 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A successful rechallenge with cetuximab for a case with metastatic rectal cancer kn-title=一次治療でセツキシマブ不耐となった後，三次治療での再使用により奏功した進行大腸癌の一例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　A 55-year-old man who had been diagnosed with rectal cancer with multiple liver metastases and lymph node metastases on colonoscopy and computed tomography (CT) was referred to Okayama University Hospital for treatment. Based on the diagnosis of non-curative rectal cancer, we planned to perform systematic chemotherapy after surgical resection. We performed a low anterior resection of a 36×35 mm upper rectal moderately differentiated adenocarcinoma with wil-type KRAS. After the resection, a FOLFIRI regimen with cetuximab was given as the first-line chemotherapy. Although metastatic lesions in the liver showed shrinkage, we decided to switch regimens because of intolerable adverse events. A modified FOLFOX6 regimen with bevacizumab was administered as the second-line treatment. There were no signs of disease progression until eight months later, when positron emission tomography (PET)/CT scans revealed that the new metastatic lesions appeared. As the third-line treatment, an irinotecan with cetuximab regimen was administered, leading to a good response for over 12 months. 　We experienced a successful rechallenge with cetuximab for a case with metastatic rectal cancer. For patients with wild-type KRAS colorectal cancer, rechallenge with cetuximab-based chemotherapy can be an effective therapeutic option. en-copyright= kn-copyright= en-aut-name=InadaRyo en-aut-sei=Inada en-aut-mei=Ryo kn-aut-name=稲田涼 kn-aut-sei=稲田 kn-aut-mei=涼 aut-affil-num=1 ORCID= en-aut-name=NagasakaTakeshi en-aut-sei=Nagasaka en-aut-mei=Takeshi kn-aut-name=永坂岳司 kn-aut-sei=永坂 kn-aut-mei=岳司 aut-affil-num=2 ORCID= en-aut-name=MoriYoshiko en-aut-sei=Mori en-aut-mei=Yoshiko kn-aut-name=母里淑子 kn-aut-sei=母里 kn-aut-mei=淑子 aut-affil-num=3 ORCID= en-aut-name=UmedaYuzo en-aut-sei=Umeda en-aut-mei=Yuzo kn-aut-name=楳田祐三 kn-aut-sei=楳田 kn-aut-mei=祐三 aut-affil-num=4 ORCID= en-aut-name=KubotaNobuhito en-aut-sei=Kubota en-aut-mei=Nobuhito kn-aut-name=久保田暢人 kn-aut-sei=久保田 kn-aut-mei=暢人 aut-affil-num=5 ORCID= en-aut-name=MorikawaTatsuya en-aut-sei=Morikawa en-aut-mei=Tatsuya kn-aut-name=森川達也 kn-aut-sei=森川 kn-aut-mei=達也 aut-affil-num=6 ORCID= en-aut-name=KondoYoshitaka en-aut-sei=Kondo en-aut-mei=Yoshitaka kn-aut-name=近藤喜太 kn-aut-sei=近藤 kn-aut-mei=喜太 aut-affil-num=7 ORCID= en-aut-name=UnoFutoshi en-aut-sei=Uno en-aut-mei=Futoshi kn-aut-name=宇野太 kn-aut-sei=宇野 kn-aut-mei=太 aut-affil-num=8 ORCID= en-aut-name=SadamoriYu en-aut-sei=Sadamori en-aut-mei=Yu kn-aut-name=貞森裕 kn-aut-sei=貞森 kn-aut-mei=裕 aut-affil-num=9 ORCID= en-aut-name=YagiTakahito en-aut-sei=Yagi en-aut-mei=Takahito kn-aut-name=八木孝仁 kn-aut-sei=八木 kn-aut-mei=孝仁 aut-affil-num=10 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name=藤原俊義 kn-aut-sei=藤原 kn-aut-mei=俊義 aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=8 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=9 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=10 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=11 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 en-keyword=大腸癌（colorectal cancer） kn-keyword=大腸癌（colorectal cancer） en-keyword=化学療法（chemotherapy） kn-keyword=化学療法（chemotherapy） en-keyword=セツキシマブ再投与（cetuximab rechallenge） kn-keyword=セツキシマブ再投与（cetuximab rechallenge） END start-ver=1.4 cd-journal=joma no-vol=125 cd-vols= no-issue=1 article-no= start-page=47 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of metastatic cecal cancer with mutation in the BRAF oncogene and poor survival kn-title=予後不良な経過をたどったBRAF遺伝子変異を伴うStage Ⅳ大腸癌の１例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　A 79-year-old woman visited a previous hospital with a complaint of general fatigue. The patient was diagnosed with cecal cancer with multiple liver metastases and lymph node metastases on colonoscopy, abdominal ultrasonography and CT scan, and was referred to our division for treatment. Based on the diagnosis of non-curative colonic cancer, we planned to perform systematic chemotherapy after local surgical treatment. We performed an ileocecal resection, and the specimen showed poorly differentiated adenocarcinoma with mutation in the BRAF oncogene. After the surgical treatment, the tumor grew rapidly and the patient died from cancer on the 19th postoperative day without having the opportunity to undergo chemotherapy. 　Multiple genetic and epigenetic alterations in oncogenes and tumor suppressor genes are involved in the process of colorectal carcinogenesis. Some of the alterations have been identified as predictive and prognostic biomarkers. A mutation in the BRAF oncogene was reported to be associated with a very unfavorable prognosis in colorectal cancers. Some of the cases with rapid progression are suggested to have the BRAF oncogene mutation. According to our experience, chemotherapy before surgical treatment might improve the prognosis of cases with the BRAF mutation. en-copyright= kn-copyright= en-aut-name=InadaRyo en-aut-sei=Inada en-aut-mei=Ryo kn-aut-name=稲田涼 kn-aut-sei=稲田 kn-aut-mei=涼 aut-affil-num=1 ORCID= en-aut-name=NagasakaTakeshi en-aut-sei=Nagasaka en-aut-mei=Takeshi kn-aut-name=永坂岳司 kn-aut-sei=永坂 kn-aut-mei=岳司 aut-affil-num=2 ORCID= en-aut-name=TakeharaKiyoto en-aut-sei=Takehara en-aut-mei=Kiyoto kn-aut-name=竹原清人 kn-aut-sei=竹原 kn-aut-mei=清人 aut-affil-num=3 ORCID= en-aut-name=SugiharaMasahiro en-aut-sei=Sugihara en-aut-mei=Masahiro kn-aut-name=杉原正大 kn-aut-sei=杉原 kn-aut-mei=正大 aut-affil-num=4 ORCID= en-aut-name=MoriYoshiko en-aut-sei=Mori en-aut-mei=Yoshiko kn-aut-name=母里淑子 kn-aut-sei=母里 kn-aut-mei=淑子 aut-affil-num=5 ORCID= en-aut-name=UmedaYuzo en-aut-sei=Umeda en-aut-mei=Yuzo kn-aut-name=楳田祐三 kn-aut-sei=楳田 kn-aut-mei=祐三 aut-affil-num=6 ORCID= en-aut-name=KubotaNobuhito en-aut-sei=Kubota en-aut-mei=Nobuhito kn-aut-name=久保田暢人 kn-aut-sei=久保田 kn-aut-mei=暢人 aut-affil-num=7 ORCID= en-aut-name=MorikawaTatsuya en-aut-sei=Morikawa en-aut-mei=Tatsuya kn-aut-name=森川達也 kn-aut-sei=森川 kn-aut-mei=達也 aut-affil-num=8 ORCID= en-aut-name=KondoYoshitaka en-aut-sei=Kondo en-aut-mei=Yoshitaka kn-aut-name=近藤喜太 kn-aut-sei=近藤 kn-aut-mei=喜太 aut-affil-num=9 ORCID= en-aut-name=UnoFutoshi en-aut-sei=Uno en-aut-mei=Futoshi kn-aut-name=宇野太 kn-aut-sei=宇野 kn-aut-mei=太 aut-affil-num=10 ORCID= en-aut-name=SadamoriYu en-aut-sei=Sadamori en-aut-mei=Yu kn-aut-name=貞森裕 kn-aut-sei=貞森 kn-aut-mei=裕 aut-affil-num=11 ORCID= en-aut-name=YagiTakahito en-aut-sei=Yagi en-aut-mei=Takahito kn-aut-name=八木孝仁 kn-aut-sei=八木 kn-aut-mei=孝仁 aut-affil-num=12 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name=藤原俊義 kn-aut-sei=藤原 kn-aut-mei=俊義 aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=8 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=9 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=10 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=11 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=12 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=13 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 en-keyword=大腸癌（colorectal cancer） kn-keyword=大腸癌（colorectal cancer） en-keyword=BRAF変異（BRAF mutation） kn-keyword=BRAF変異（BRAF mutation） en-keyword=化学療法（chemotherapy） kn-keyword=化学療法（chemotherapy） en-keyword=分子標的薬（molecular target therapy） kn-keyword=分子標的薬（molecular target therapy） END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=3 article-no= start-page=243 end-page=247 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20121203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of intracystic carcinoma of the breast showing pathological complete response by neoadjuvant chemotherapy kn-title=術前化学療法により壁が菲薄化し組織学的寛解（pCR）となった巨大嚢胞内乳癌の１例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　A 31-year-old woman presented to our hospital because of a lump 5cm in diameter in her right breast. Ultrasonography revealed a multilocular cystic mass with partial thickening of the wall and the septum. Core needle biopsy (CNB) of the septum confirmed invasive ductal carcinoma. The patient was administered neoadjuvant chemotherapy consisting of 12 cycles of weekly paclitaxel followed by 4 cycles of FEC (5FU, Epilubicin and cyclophosphamide). While the thickness of the wall and septum became thinner, the tumor size did not change in diameter. She underwent right mastectomy and sentinel node biopsy. Post-surgical histopathological findings showed a pathological complete response (pCR).
　We report a rare case of invasive ductal carcinoma presenting a multilobular phenotype which showed pCR by neoadjuvant chemotherapy despite no size reduction of the tumor. en-copyright= kn-copyright= en-aut-name=ImadaTakako en-aut-sei=Imada en-aut-mei=Takako kn-aut-name=今田孝子 kn-aut-sei=今田 kn-aut-mei=孝子 aut-affil-num=1 ORCID= en-aut-name=MatsuokaJunji en-aut-sei=Matsuoka en-aut-mei=Junji kn-aut-name=松岡順治 kn-aut-sei=松岡 kn-aut-mei=順治 aut-affil-num=2 ORCID= en-aut-name=MotokiTakayuki en-aut-sei=Motoki en-aut-mei=Takayuki kn-aut-name=元木崇之 kn-aut-sei=元木 kn-aut-mei=崇之 aut-affil-num=3 ORCID= en-aut-name=IwamotoTakayuki en-aut-sei=Iwamoto en-aut-mei=Takayuki kn-aut-name=岩本高行 kn-aut-sei=岩本 kn-aut-mei=高行 aut-affil-num=4 ORCID= en-aut-name=OomoriMasako en-aut-sei=Oomori en-aut-mei=Masako kn-aut-name=大森昌子 kn-aut-sei=大森 kn-aut-mei=昌子 aut-affil-num=5 ORCID= en-aut-name=IhaSigemichi en-aut-sei=Iha en-aut-mei=Sigemichi kn-aut-name=伊波茂道 kn-aut-sei=伊波 kn-aut-mei=茂道 aut-affil-num=6 ORCID= en-aut-name=HongoToshie en-aut-sei=Hongo en-aut-mei=Toshie kn-aut-name=本後登志江 kn-aut-sei=本後 kn-aut-mei=登志江 aut-affil-num=7 ORCID= en-aut-name=HenmiNoriko en-aut-sei=Henmi en-aut-mei=Noriko kn-aut-name=逸見典子 kn-aut-sei=逸見 kn-aut-mei=典子 aut-affil-num=8 ORCID= en-aut-name=MakabeMikio en-aut-sei=Makabe en-aut-mei=Mikio kn-aut-name=真壁幹夫 kn-aut-sei=真壁 kn-aut-mei=幹夫 aut-affil-num=9 ORCID= en-aut-name=NogamiHiromi en-aut-sei=Nogami en-aut-mei=Hiromi kn-aut-name=野上浩實 kn-aut-sei=野上 kn-aut-mei=浩實 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=5 en-affil= kn-affil=岡山大学病院　病理診断科 affil-num=6 en-affil= kn-affil=岡山大福クリニック affil-num=7 en-affil= kn-affil=岡山大福クリニック affil-num=8 en-affil= kn-affil=岡山大福クリニック affil-num=9 en-affil= kn-affil=岡山中央病院 affil-num=10 en-affil= kn-affil=野上病院 en-keyword=嚢胞内乳癌（intracystic breast cancer） kn-keyword=嚢胞内乳癌（intracystic breast cancer） en-keyword=術前化学療法（neoadjuvant chemotherapy） kn-keyword=術前化学療法（neoadjuvant chemotherapy） en-keyword=組織学的完全奏功（pahthological CR） kn-keyword=組織学的完全奏功（pahthological CR） END start-ver=1.4 cd-journal=joma no-vol=108 cd-vols= no-issue=4 article-no= start-page=043916-1 end-page=043916-4 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100815 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Bulk and surface physical properties of a CrO2 thin film prepared from a Cr8O21 precursor en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have prepared a CrO(2) thin film by chemical vapor deposition from a Cr(8)O(21) precursor and studied the bulk and surface physical properties. The CrO(2) thin film is grown on a TiO(2) (100) substrate by heating of a Cr(8)O(21) precursor and TiO(2) (100) substrate together in a sealed quartz tube. The prepared film is found from x-ray diffraction analysis to be an (100)-oriented single phase. The magnetization and resistivity measurements indicate that the film is a ferromagnetic metal with a Curie temperature of about 400 K. Cr 3s core-level and valence band photoelectron spectroscopy spectra reveal the presence of a metallic CrO(2) in the surface region of the film. Our work indicates that preparation from a Cr(8)O(21) precursor is promising for obtaining a CrO(2) thin film with the metallic surface. en-copyright= kn-copyright= en-aut-name=IwaiK. en-aut-sei=Iwai en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MuraokaY. en-aut-sei=Muraoka en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WakitaT. en-aut-sei=Wakita en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HiraiM. en-aut-sei=Hirai en-aut-mei=M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YokoyaT. en-aut-sei=Yokoya en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KatoY. en-aut-sei=Kato en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MuroT. en-aut-sei=Muro en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TamenoriY. en-aut-sei=Tamenori en-aut-mei=Y. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Faculty of Science, Research Laboratory for Surface Science, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=Japan Synchrotron Radiation Research Institute (JASRI)/SPring-8 affil-num=7 en-affil= kn-affil=Japan Synchrotron Radiation Research Institute (JASRI)/SPring-8 affil-num=8 en-affil= kn-affil=Japan Synchrotron Radiation Research Institute (JASRI)/SPring-8 END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=2 article-no= start-page=149 end-page=153 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of strangulated ileus caused by transomental hernia with reference to previously reported cases kn-title=術前診断し得た大網裂孔ヘルニアの一例―本邦報告203例の臨床病理学的検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Transomental hernia often develops into strangulated ileus. We report on an 81-year-old man with strangulated ileus due to transomental hernia, diagnosed preoperatively by abdominal CT. The patient was referred to our surgical division because of progressive abdominal pain and vomiting. He had no history of laparotomy. An abdominal CT scan showed dilated small intestinal loops with intraluminal air and strangulated small intestinal loops with engorged mesenteric vessels. We diagnosed this as a strangulated internal hernia due to transomental hernia and conducted an emergency laparotomy. The jejunum had herniated through an abnormal hiatus of the greater omentum to the peritoneal cavity. The strangulated intestinal loop, about 15 cm long, was released and the postoperative course was uneventful. In the absence of a previous laparotomy, the differential diagnosis of intestinal obstruction should include internal hernia. An abdominal CT scan is useful for the preoperative and prompt diagnosis of transomental hernia. en-copyright= kn-copyright= en-aut-name=KimuraYuji en-aut-sei=Kimura en-aut-mei=Yuji kn-aut-name=木村裕司 kn-aut-sei=木村 kn-aut-mei=裕司 aut-affil-num=1 ORCID= en-aut-name=IwakawaKazuhide en-aut-sei=Iwakawa en-aut-mei=Kazuhide kn-aut-name=岩川和秀 kn-aut-sei=岩川 kn-aut-mei=和秀 aut-affil-num=2 ORCID= en-aut-name=NishieManabu en-aut-sei=Nishie en-aut-mei=Manabu kn-aut-name=西江学 kn-aut-sei=西江 kn-aut-mei=学 aut-affil-num=3 ORCID= en-aut-name=InagakiMasaru en-aut-sei=Inagaki en-aut-mei=Masaru kn-aut-name=稲垣優 kn-aut-sei=稲垣 kn-aut-mei=優 aut-affil-num=4 ORCID= en-aut-name=IwagakiHiromi en-aut-sei=Iwagaki en-aut-mei=Hiromi kn-aut-name=岩垣博巳 kn-aut-sei=岩垣 kn-aut-mei=博巳 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=国立病院機構福山医療センター　外科 affil-num=2 en-affil= kn-affil=国立病院機構福山医療センター　外科 affil-num=3 en-affil= kn-affil=国立病院機構福山医療センター　外科 affil-num=4 en-affil= kn-affil=国立病院機構福山医療センター　外科 affil-num=5 en-affil= kn-affil=国立病院機構福山医療センター　外科 en-keyword=大網裂孔ヘルニア（transomental hernia） kn-keyword=大網裂孔ヘルニア（transomental hernia） en-keyword=絞扼性イレウス（strangulated ileus） kn-keyword=絞扼性イレウス（strangulated ileus） END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=1 article-no= start-page=63 end-page=66 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pathological complete response of advanced gastric cancer with pyloric stenosis to neoadjuvant S-1/CDDP chemotherapy: A case report kn-title=S-1/CDDP術前化学療法により組織学的CRが得られた幽門狭窄合併進行胃癌の１例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 59-year-old man with epigastric discomfort and anorexia was referred to our hospital. Endoscopy revealed a type 3 advanced gastric cancer with pyloric stenosis diagnosed as a poorly differentiated adenocarcinoma in the biopsy specimens. A gastrojejunal bypass operation was performed because of direct invasion to the pancreas. The patient was treated by three courses of neoadjuvant chemotherapy with S-1/CDDP. Follow-up abdominal CT scan revealed that the primary tumor had become smaller, suggesting that a partial response had been achieved. Distal gastrectomy with D2 lymphadenectomy was performed. The histopathological examination showed no residual cancer cells in the primary lesion or dissected lymph nodes. Final chemotherapy efficacy was evaluated as Grade 3. The patient was treated with S-1 for one year after the gastrectomy and lymphadenectomy and has been followed up for 18 months without evidence of recurrence. en-copyright= kn-copyright= en-aut-name=NishizakiMasahiko en-aut-sei=Nishizaki en-aut-mei=Masahiko kn-aut-name=西崎正彦 kn-aut-sei=西崎 kn-aut-mei=正彦 aut-affil-num=1 ORCID= en-aut-name=FujiwaraYasuhiro en-aut-sei=Fujiwara en-aut-mei=Yasuhiro kn-aut-name=藤原康宏 kn-aut-sei=藤原 kn-aut-mei=康宏 aut-affil-num=2 ORCID= en-aut-name=ChoudaYasuhiro en-aut-sei=Chouda en-aut-mei=Yasuhiro kn-aut-name=丁田泰宏 kn-aut-sei=丁田 kn-aut-mei=泰宏 aut-affil-num=3 ORCID= en-aut-name=KanazawaTakashi en-aut-sei=Kanazawa en-aut-mei=Takashi kn-aut-name=金澤卓 kn-aut-sei=金澤 kn-aut-mei=卓 aut-affil-num=4 ORCID= en-aut-name=NinomiyaMotoki en-aut-sei=Ninomiya en-aut-mei=Motoki kn-aut-name=二宮基樹 kn-aut-sei=二宮 kn-aut-mei=基樹 aut-affil-num=5 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name=藤原俊義 kn-aut-sei=藤原 kn-aut-mei=俊義 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 affil-num=2 en-affil= kn-affil=広島市立広島市民病院　外科 affil-num=3 en-affil= kn-affil=広島市立広島市民病院　外科 affil-num=4 en-affil= kn-affil=広島市立広島市民病院　外科 affil-num=5 en-affil= kn-affil=広島市立広島市民病院　外科 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器外科学 en-keyword=幽門狭窄 (pyloric stenosis) kn-keyword=幽門狭窄 (pyloric stenosis) en-keyword=進行胃癌 (advanced gastric cancer) kn-keyword=進行胃癌 (advanced gastric cancer) en-keyword=S-1/CDDP kn-keyword=S-1/CDDP en-keyword=術前化学療法 (neoadjuvant chemotherapy) kn-keyword=術前化学療法 (neoadjuvant chemotherapy) en-keyword=組織学的CR (pathological CR) kn-keyword=組織学的CR (pathological CR) END start-ver=1.4 cd-journal=joma no-vol=32 cd-vols= no-issue=4 article-no= start-page=365 end-page=372 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=1990 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Assay of GBM antigen in urine and serum with an anti-human renal monoclonal antibody kn-title=抗ヒト腎モノクローナル抗体を用いた尿中及び血中のGBM抗原の測定 第2編 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using a monoclonal antibody (Mab-G3) recognizing glomerular basement membrane (GBM), we assayed GBM antigen (G3-Ag) in the urine and serum of renal disease patients by sandwich ELISA. The subjects included normal control (NOR), minimal change nephrotic syndrome (MCNS), IgA nephropathy (IgA), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN) and chronic renal failure (CRF). The urine and serum was used as the material. With urinary G3-Ag, there were no statistically significant differences among the NOR, MCNS, IgA, MN, MPGN and CRF groups. Although no correlation was observed with proteinuria, hematuria, serum creatinine, serum β2 microglobulin and urinary NAG, urinary G3-Ag showed a significant (p<0.05) increase in excretion in the group of progressive CRF patients with s-Cr more than 1.0 mg/dl/month as compared to the stationary CRF group with s-Cr<1.0 mg/dl/month. Serum G3-Ag showed lower values in almost all cases, and there were no significant differences among the renal disease groups. The above findings led us to believe that the assay of urinary G3-Ag was useful in determining the degree of GBM disorder. It was also presumed that assay of renal antigens in urine and serum with the respective anti-human renal monoclonal antibodies could be a new tool in diagnosing renal diseases. en-copyright= kn-copyright= en-aut-name=MinoYasuaki en-aut-sei=Mino en-aut-mei=Yasuaki kn-aut-name=味埜泰明 kn-aut-sei=味埜 kn-aut-mei=泰明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第三内科 en-keyword=monoclonal antibody kn-keyword=monoclonal antibody en-keyword=sandwich ELISA kn-keyword=sandwich ELISA en-keyword=GBM antigen kn-keyword=GBM antigen en-keyword=renal disease kn-keyword=renal disease END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue=5 article-no= start-page=455 end-page=465 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=1989 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Spontaneous Cell Mediated Cytotoxicity (SCMC) against Rat Skin Epidermal Cells - Comparison of Natural Cytotoxicity Cell (NC) with Killer Cell (KC) - kn-title=ラット皮膚細胞に対する natural cytotoxicity (SCMC) の検討―natural cytotoxicity 細胞(NC)とキラー細胞(KC)との比較 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We have reported that normal rat spleen cells show spontaneous cell-mediated cytotoxicity (SCMC) against rat skin epidermal cells and that the cold target inhibition assay reveals that NC and immune spleen cells belonged to different cell populations. This time, we attempted to separate NC and KC from skin allografted immune spleen cells by using Percoll sedimentation and compare their cytotoxicity with that of normal spleen cells. Normal and skin grafted immune spleen cells were fractionated by 30-50% (grading by 5%) Percoll, and each fraction was used as an effector in the (51)Cr release cytotoxicity assay with the target of allogeneic or syngeneic rat tail epidermal cells. The results were; Fr. 40% as NC and Fr. 50% as KC population. Cytotoxicity of nonadherent cells to the plastic dish and nylon wool column showed approximately the same distribution. Cold target inhibition assay was then performed with each fraction, confirming the results in our previous report. Each fraction was treated with antiasialo GM1 serum and complement and drastic elimination of the cytotoxicity was seen only in Fr. 40%. In addition, cytotoxicity of Fr. 40% and Fr. 50% of 0, 1, 2, 3, 4, 7, 10, 13 and 16th days, following the syngeneic and allogeneic skin graft, was mointored in order to determine the kinetics of NC and KC activities. en-copyright= kn-copyright= en-aut-name=FujimotoHideo en-aut-sei=Fujimoto en-aut-mei=Hideo kn-aut-name=藤本英雄 kn-aut-sei=藤本 kn-aut-mei=英雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=First Department of Surgery, Okayama University Medical School en-keyword=natural cytotoxicity cell kn-keyword=natural cytotoxicity cell en-keyword=killer cell kn-keyword=killer cell en-keyword=Percoll sedimentation kn-keyword=Percoll sedimentation END start-ver=1.4 cd-journal=joma no-vol=123 cd-vols= no-issue=3 article-no= start-page=231 end-page=235 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20111201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Japanese guidelines for the diagnosis and management of soft tissue tumor kn-title=軟部腫瘍診療ガイドライン en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MorimotoYuki en-aut-sei=Morimoto en-aut-mei=Yuki kn-aut-name=森本裕樹 kn-aut-sei=森本 kn-aut-mei=裕樹 aut-affil-num=1 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name=尾﨑敏文 kn-aut-sei=尾﨑 kn-aut-mei=敏文 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学病院　整形外科 affil-num=2 en-affil= kn-affil=岡山大学病院　整形外科 END start-ver=1.4 cd-journal=joma no-vol=116 cd-vols= no-issue=1350 article-no= start-page=220 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Chemical recycling of inorganic wastes by using phase separation of glass en-subtitle= kn-subtitle= en-abstract= kn-abstract=A chemical recycling process using phase separation of glass was applied to a granulated blast furnace slag with high CaO content. Glasses were prepared by adding B(2)O(3) to the slag in order to promote phase separation, and the glasses were heat-treated above glass transition temperature. In the subsequent acid-treatment, however, gelation due to the elution of SiO(2) phase containing CaO occurred, not obtaining high SiO(2) solids. Then, pretreatment was introduced to reduce CaO content in slag, where the raw slag was briefly washed in acid. The slag glasses prepared from the pretreated slag were commonly phase-separated by heat-treatment regardless of B(2)O(3) content. After subsequent acid-treatment, colorless insoluble solids were successfully recovered. The end products consist of 70-90 mass% SiO(2), and coloring ions such as Mn, Fe and Cr were almost completely removed from the slag glasses. Reutilization as raw materials for glass is expected. en-copyright= kn-copyright= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MikamiShuhei en-aut-sei=Mikami en-aut-mei=Shuhei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ImaokaTakuya en-aut-sei=Imaoka en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakidaShinichi en-aut-sei=Sakida en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiuraYoshinari en-aut-sei=Miura en-aut-mei=Yoshinari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Environmental Science, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Environmental Science, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Environmental Science, Okayama University affil-num=4 en-affil= kn-affil=Health and Environment Center, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Environmental Science, Okayama University en-keyword=Blast furnace slag kn-keyword=Blast furnace slag en-keyword=Waste recycling kn-keyword=Waste recycling en-keyword=Chemical recycling kn-keyword=Chemical recycling en-keyword=Phase separation kn-keyword=Phase separation en-keyword=Glass kn-keyword=Glass END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=4 article-no= start-page=231 end-page=237 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Clinical Efficacy of Capecitabine and Cyclophosphamide (XC) in Patients with Metastatic Breast Cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=Combined low-dose therapy of oral capecitabine (Xeloda) and cyclophosphamide (XC) has been demonstrated to be useful for long-term control of lesions in patients with metastatic breast cancer (MBC) and is aimed at symptomatic alleviation and prolongation of survival. Here, a retrospective review was conducted of MBC patients administered XC at the Okayama University Hospital (OUH), to evaluate responses to XC, adverse events and time to progression (TTP). Twenty patients with MBC received XC between 2006 and 2009. With the exception of 2 elderly patients who were over the age of 70 at the initial examination, all of the patients had received prior treatment with an anthracycline and/or a taxane. No complete response (CR) cases were observed, but partial response (PR) was achieved in 6 patients (30%) and SD in 9 (45%), of whom 5 (20%) sustained SD status for >12 months. The median TTP was 6 months (range:3-27 mo.). Three patients developed Grade 3 adverse events (diarrhea, nausea and stomatitis), but no other patients developed adverse reactions causing interruption of the therapy. XC was safe even in previously treated and elderly MBC patients;moreover, it yielded remarkable clinical responses. en-copyright= kn-copyright= en-aut-name=ShienTadahiko en-aut-sei=Shien en-aut-mei=Tadahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DoiharaHiroyoshi en-aut-sei=Doihara en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishiyamaKeiko en-aut-sei=Nishiyama en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MasudaHiroko en-aut-sei=Masuda en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NogamiTomohiro en-aut-sei=Nogami en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IkedaHirokuni en-aut-sei=Ikeda en-aut-mei=Hirokuni kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TairaNaruto en-aut-sei=Taira en-aut-mei=Naruto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=2 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=3 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=4 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=5 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=6 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital affil-num=7 en-affil= kn-affil=Department of Breast and Endocrine Surgery, Okayama University Hospital en-keyword=metastatic breast cancer kn-keyword=metastatic breast cancer en-keyword=metronomic kn-keyword=metronomic en-keyword=chemotherapy kn-keyword=chemotherapy END start-ver=1.4 cd-journal=joma no-vol=40 cd-vols= no-issue=5 article-no= start-page=985 end-page=999 dt-received= dt-revised= dt-accepted= dt-pub-year=1928 dt-pub=19280531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die Färbung des Glykogens im tierischen Gewebe (1. Mitteilung) Färbuugsergebnisse des Glykogens mit basischen und sauren Teerfarbstoffen kn-title=「グリコゲーン」染色ニ就テ (第1囘報告) 鹽基性及ビ酸性「テール」色素ヲ以テスル「グリコゲーン」染色ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Während die histologische Untersuchung des Glykogens in tierischen Geweben von verschiedenen Seiten vielfach unternommen wurde, sind wir doch über den Färbe-Prozess desselben Bestandteils nur ungenügend unterrichtet. Daher habe ich mich mit diesem Thema beschäftigt und einige Ergebnisse erzielt, welche ich als meine erste Mitteilung veröffentlichen will. Sie lauten wie folgt: A. Ausser wenn das indirekte Färbungsverfahren nach A. Fischer, wobei ich übrigens anstatt der Kaliumbichromatlösung eine Lösung von Stibio-Kalium tartaricum mit gutem Erfolg brauchen konnte, verwendet wird, vermag man das Glykogen im Gewebe gleichgültig nicht mit basischen Teerfarbstoffen zu färben. Selbst wenn man dabei den Schnitt mit einer alkalischen Lösung vor-bzw. nachbehandelt, lässt sich diese Ingredienz nicht elektiv färben. B. Dagegen kann man mit manchen sauren Farbstoffen wie Säurefuchsin, Trypanblau, Bleu de Lyon, Anilinblau, Wasserblau usw. Glykogen direkt tingieren, u. z. mit gutem Erfolge. In diesem Falle färben sich aber auch die Zellkerne mehr oder weniger intensiv. C. Bei der indirekten Glykogenfärbung nach Fischer kann man anstatt der basischen auch manche saure Farbstoffe wie Säurefuchsin, Eosin, Azur II, Anilinblau etc. ohne Nachteil gut anwenden. D. Viele saure Farbstoffe, besonders Eosin, Orange G, Säurefuchsin, Trypanblau und Kongorot färben Glykogen stärker mit Hilfe der metallischen Beizen wie Al, Fe, Cr, Cu etc. E. Lässt man auf den Schnitt nach bzw. vor der Färbung eine Säurelöosung einwirken, so erhöht sich in der Regel die Färbungskraft der sauren Farbstoffe. Doch kann man diese Tatsache zur Glykogenfärbung nicht ausnützen, denn man erzielt dadurch nur eine allgemeine intensive Färbung, so dass Glykogen sich nicht scharf abhebt. Eine Ausnahme bildet die Glykogenfärbung mit Kongorot, welche durch eine Vor- bzw. Nachbehandlung mit Pikrinsäure sehr hübsch ausfällt. F. Die Färbung des Glykogens mit Kongorot ergibt sehr verschiedene Resultate je nach der Prozedur. 1. Wenn man einen mit Kongorot 10 Minuten lang gefärbten Schnitt in Äthyl-oder Methylalkohol legt und dann mit Xylol aufhellt, so erhält man ein vorzügliches Präparat für die Glykogenfärbung. Im allgemeinen wirkt reines Xylol auf die Glykogenfärbung besser als Karbolxylol. 2. Wenn ein mit Kongorot 10 Minuten lange gefärbter Schnitt in Methyl- oder Äthylalkohol gelegt und darauf der Wirkung des salzsäurehaltigen Alkohols unterworfen wird, so bekommt man ein Präparat von blauschwärzlichem Farbenton, worin aber Glykogen durch seine intensivere Färbung besonders ausgezeichnet ist. Dabei fällt die Kernfärbung viel günstiger aus, wenn man statt des Methylalkohols Äthylalkohol anwendet. 3. Wenn man einen mit Kongorot 10 Minuten lang gefärbten Schnitt zuerst in Wasser auswäscht und dann mit Alkohol entwässert, so ergibt sich die Glykogenfärbung immer sehr schlecht. Dies ist, m. E., darauf zurückzuführen, dass der absorbierte Farbstoff vom Glykogen leicht ins Wasser ausspringt und dadurch weggespült wird. 4. Dagegen kann man den nach Nr. 2 behandelten Schnitt ruhig in Wasser answaschen und dann mit Alkohol entwässern. In diesem Fall lässt sich ein gutes oder noch schöneres Präparat darstellen als bei den anderen Verfahren. 5. Durch Zusatz von Säure nehmen die mit Kongorot gefärbten Schnitte, wie gesagt, immer einen blauen Farbenton an. Dieser Farbenton verwandelt sich aber durch die Abspülung mit Leitungswasser wieder in die ursprüngliche rote Farbe, während dies bei der Auswaschung in reinem destilliertem Wasser keineswegs der Fall ist. Es liegt auf der Hand, dass der Umschlag der Farbe auf der alkalischen Reaktion des Leitungswassers beruht. en-copyright= kn-copyright= en-aut-name=YuienKazue en-aut-sei=Yuien en-aut-mei=Kazue kn-aut-name=結縁主計 kn-aut-sei=結縁 kn-aut-mei=主計 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學解剖學教室 END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=1 article-no= start-page=125 end-page=131 dt-received= dt-revised= dt-accepted= dt-pub-year=1936 dt-pub=19360131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimentelle Untersuchung über den Geschmack (I. Mitteilung.) Über die Vorgänge an der peripheren Sinnesfläche des Geschmacksorganes. (Anhang; Über die Unterschiedsempfindlichkeit des Geschmackes) kn-title=味覺ニ關スル實驗的研究(第1報)末梢味覺領域ニ於ケル轉機ニ就テ(附)識別閾ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Renqvist hat für die Entstehung des Geschmackes an der peripheren Sinnesorgan folgende Gleichung aufgestellt: D · α · Cr1/n=Const. Wo D der Diffusionscoeffizient, Cr die Schwellenkonzentration für Geschmacksempfindung, α und 1/n die Adsorptionskonstanten sind. Die Ergebnisse meiner Untersuchung bezüglich seiner Gleichung lassen sich kurz, wie folgt, zusammenfassen. 1) Bei den Schwellenkonzentrationen für die Geschmacksempfindung bei einigen Zuckerarten ist D · α · Cr1/n constant und fast gleich gross. 2) Die bei der Schwelle des Geschmacksempfindung geltende Gleichung für Neutralsalze erhält die folgende Gestalt: D · Cr=Const. Verfasser schliesst aus seinen Versuchen über die Unterschiedsempfindlichkeit des Geschmackes, dass das Webersche Gesetz für den Geschmack ebenfalls gültig ist. en-copyright= kn-copyright= en-aut-name=KameiTosio en-aut-sei=Kamei en-aut-mei=Tosio kn-aut-name=龜井俊夫 kn-aut-sei=龜井 kn-aut-mei=俊夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學生理學教室 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=4 article-no= start-page=808 end-page=838 dt-received= dt-revised= dt-accepted= dt-pub-year=1937 dt-pub=19370430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über den Einfluss der Nukleinsäure anf Hamazakis Cr-säurefeste Granula bei hungernden Tieren kn-title=饑餓動物ノ濱崎「クローム」・耐酸性顆粒ニ及ボス核酸ノ影響ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In Heften hat Verf. zweimal eingehend über den Einfluss des Hungers auf die betreffenden Granula berichtet. Beim Hungern nehmen die Cr-säurefesten Granula allmahlich ab und verschwinden in der Regel nach 5- bis 13-tägigem Versuch. Hamazaki hat oft die Ansicht geäussert, dass die säurefesten Substanzen zu den Nukleoproteiden und ihren Spaltungsprodukten gehoren. Demgemäss wollte er die Wirkung der Nukleinsäure auf die Cr-säurefesten Granula des Hungernden Tieres prüfen und liess den Verf. diesbezügliche systematische Versuche vornehmen. In der ersten Reihe der Versuche bekamen die hungernden erwachsenen Kaninchen am dritten Tage des Hungers eine intravenöse Injektion nukleinsaurer Natrönlosung (1.0-1.5: 100.0), am vierten Tage bekamen sie dieselbe Injektion zum zweiten Male und 24 Stunden nach dieser letzten Injektion wurden sie durch Luftembolie getotet. In der zweiten Reihe wurde die erste Injektion am fünften Tage, die zweite am sechsten Tage verabreicht und 10 Stunden nach der letzten Injektion wurden die Versuchstiere getotet. In der dritten Reihe wurde nukleinsaures Natron per os appliziert. Als Kontrollversuche nahm Verf. intravenöse und perorale Darreichung von Traubenzuckerlosung und schliesslich intravenöse Injektion von physiologischer Köchsalzlosung vor. Die Resultate lassen sich wie folgt zusammenfassen: 1) Die durch Hunger verminderten bzw. verschwundenen Cr-säurefesten Granula konnen durch die intravenose Injektion des hefenukleinsauren Natrons wieder hergestellt werden. Ausserdem kann durch die Wiederholung derselben Injektion abnorme Steigerung der Granulazahl festgestellt werden. 2) Bei der abnormen Granulavermehrung ist zu beachten, dass die Organgewebe, die normalerweise keine oder nur etliche Granula fuhren-wie die Sammelrohrepithelien und die des dunneren Teils der Henleschen Schleife der Niere, das Knochenmark, die Drusenblaschenepithelien des Pankreas, das Kapsel-und Balkengewebe der Milz, das Interstitium des Hodens and der Leber u.a.-sehr deutlich die Cr-säurefeste Granula zeigen. 3) Die Perorale Darreichung des Hefenukleinsauren Natrons hat wenig Einfluss auf die Cr-säurefesten Granula der hungernden Tiere. 4) Die intravenose und perorale Darreichung von Traubenzucker kann eine leichte Vermehrung der Cr-säurefesten Granula der hungernden Tiere hervorrufen. en-copyright= kn-copyright= en-aut-name=KonishiNobuo en-aut-sei=Konishi en-aut-mei=Nobuo kn-aut-name=小西信雄 kn-aut-sei=小西 kn-aut-mei=信雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=2 article-no= start-page=236 end-page=262 dt-received= dt-revised= dt-accepted= dt-pub-year=1937 dt-pub=19370228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über den Einfluss des Hungers. auf Hamazakis Cr-säurefeste Granula. (I. Mitteilung.) kn-title=濱崎氏「クローム」・耐酸性顆粒ニ及ボス饑餓ノ影響ニ就テ（第1報） en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seit drei Jahren hat sich Hamazaki mittels seines eigenen Forschungsverfahrens, welches in der Fixation mit Schwermetallsalzen und der Färbung durch KarbolfuchsinJod-Methode besteh, mit den säurefesten Substanzen beschäftigt und bis jetzt vier Arten von säurefesteu Granula nachgewiesen, nämlich Cr-säurefeste, Cu-säurefeste, Fe-säurefeste und Hg-säurefeste-Granula. Diese Granula stehen in so untrennbarer Beziehung zueinander, dass sie ein neues Stoffwechselsystem auszubauen gestatten. Unter ihnen gehoren die Cr-säurefesten Granula zu einer hoheren Stufe der in Frage kommenden Substanzen. Sie verbreiten sich in fast allen Parenchpmzellen der Organe und in mesenchymalen Geweben, insbesondere sind sie in Gehirn, Herzmuskel, Dünndarmepithelien, Skelettmuskeln, Nebennieren, Nieren und Leber reichlich vorhanden. Die biologische Untersuchung der Cr-säurefesten Granula muss also reizvoll und interessant sein. Für zukünftige Untersuchungen der Granula sind Hungerversuche als Grundlage unentbehrlich. Anlässlich des 26. Japanischen Pathologenkongresses haben Hamazaki und Konishi vorlaufig über das in Frage kommende Thema berichtet und nachher hat Verf. unter der Leitung von ao. Prof. Hamazaki diesbezügliche systematische Untersuchungen vorgenommen. Als versuchstiere wurden erwachsene gesunde Kaninchen, die mit Tohukasu ernährt worden waren, benutzt. Nach dem Beginn des Hungerns wurden sie am dritten, bzw. fünften, siebenten, zehnten, zwölften und schliesslich am dreizehnten Tage durch Luftembolie getötet. In jedem Falle wurden die 35 verschiedenen in der Tabelle angegebenen Arten von Geweben und Organen frisch mit Müller-Eisessig (100:5) drei Tage lang fixiert. Nach dem Auswaschen wurden Paraffinschitte hergestellt und diese nach Hamazakis Karbolfuchsin-Jod-Methode behandelt (s. Virch. Arch. Bd. 295). Als Kontrollfärbung wurden Hämatoxylin-Eosin-Färbung und Ciaccios Lipoidnachweis durchgeführt. Beim Hungern nehmen die Cr-säurefesten Granula allmählich ab und verschwiden sich in der Regel durch 5 bis 13 tägigen Versuch. Die Cr-säurefesten Granula des lockeren Bindegewebes, des glatten Muskelgewebes und des lymphadenoiden Gewebes sind im allgemeinen am wenigsten widerstandsfähig gegen Hungerwirkungen, und binnen 5 Tagen vermindern sie sich bis zum Verschwinden. Die Granula des Herzens und des Zentralnervensystems sind ganz widerstandsfähig gegen Hungerwirkungen. Gegen das Endstadiums des Hungerns treten hie und da Degenerationsatrophien der Körperzellen auf und dann entstehen aus den betreffenden Zellkernen degenerativ viele Cr-säurefeste Granula. Am deutlichsten ist dieser Vorgang in den Mgenfundusdrüsen nachweisbar. en-copyright= kn-copyright= en-aut-name=KonishiNobuo en-aut-sei=Konishi en-aut-mei=Nobuo kn-aut-name=小西信雄 kn-aut-sei=小西 kn-aut-mei=信雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=9 article-no= start-page=1878 end-page=1892 dt-received= dt-revised= dt-accepted= dt-pub-year=1938 dt-pub=19380930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über Hamazakische säurefeste Granula der normalen Ratte. (II. Mitteilung) Studien äber Hg-säurefeste Granula, insbesondere uber die Säurefestigkeit von Zellkernen des Drüsenepithels. kn-title=正常白鼠ノ濱崎氏耐酸性顆粒ノ研究（第2報）汞・耐酸性顆粒ニ就テ，殊ニ腺細胞核ノ耐酸性ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Durch eingehende Untersuchungen beim Kaninchen hat Hamazaki bereits klargemacht, daß die Hg-säurefesten Granula als Hauptbestandteil Purinbasen enthalten, und nicht nur vom histologischen, sondern auch vom morphologischen Standpunkt aus einen großen Unterschied von den 3 andern Hamazakischen. säurefesten Granula zeigen. Der Verfasser, der bei seinern letzten Untersuchungen der Cr-säurefesten Granula ausfuhrlich studied hatte, stellte diesmal systematische histologische Untersuchungen über die oben genannten charakteristischen Hg-säurefesten Granula an. Es ergaben sich folgende interestante Resultate: 1) Bei der Ratte kommen die Hg-säurefesten Granula in geringerer Zahl und in beschränkterer Verteilung vor als die Cr-säurefesten Granula. 2) Bei der Darstellung der Hg-säurefesten Granula ist das Lipoid im allgemeinen nicht so häufig fixierbar wie beim Nachweis der Cr-säurefesten Granula. 3) Bei der Fixation der Hg-säurefesten Granula werden eine amorphe säurefeste Substanz und ein merkuraffines Korperchen, welche in den 3 andern Hamazakischen säurefesten Granula nicht nachgewiesen werden konnen, dargestellt. Sie kommen in den Rattengeweben bei weitem nicht so haufig vor wie beim Kaninchen. 4) In den Kernen der Drüsenzellen, wie in den der Submaxillardräse, der Magenschleimhaut, des Leber- und Pankreasparenchyms, des Harnkanalchens, der Blinddarmschleimhaut und Hypophysenvorderlappens findet man die saurefesten Granula sehr oft. 5) Die säurefesten Granula im Zellkerne zeigen sich abwechselnd mit den Granula des betreffenden Protoplasmas, was darauf hindeutet, deß eine intime Korrelation zwischen den Funktionen der Zelle und des Kerns besteht. 6) Im allgemeinen kann man, abgesehen von den obengenannten Drüsenzellen, in Zellkernen keine saurefesten Granula nachweisen. Sie werden aber ausnahmsweise in Nervenzellen gefunden. Dies ist wahrscheinlich darauf zurückzufuhren, daß die Nervenzelle im postfoetalen Leben keine Kernteilung durchmachen kann. 7) Die säurefeste Substanz des Kerns findet sich am haufigsten im Kernkorperchen. Diese Tatsache stimmt mit der alten Theorie vollig uberein, daß das Kernkorperchen die Spaltungsprodukten der Kernsubstanz enthalt. 8) Im Myometrium der Ratte kann man diejenige Wanderzellen, die den Hamazakischen Glanzzellen sehr ahnlich sind, selten nachweisen. Nach Meinung des Verf. konmen dieselben Zellen unter bestimmten Bedingungen. auch in tierischen Geweben auftreten. en-copyright= kn-copyright= en-aut-name=SugaYukio en-aut-sei=Suga en-aut-mei=Yukio kn-aut-name=菅幸雄 kn-aut-sei=菅 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學耳鼻咽喉科教室 END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=7 article-no= start-page=1444 end-page=1458 dt-received= dt-revised= dt-accepted= dt-pub-year=1938 dt-pub=19380731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die Beziehung zwischen den Hamazakischen säurefesten Granula und der durch Natriumsilikatinjektion erzeugten Amyloidosis. (2. Mitteilung) Dio Cu-säurefesten Grannla und die Bg-säurefesten Grannla kn-title=濱崎氏耐酸牲顆粒ト硅曹注射ニ依ルAmyloidosisトノ關係ニ就テ（第2報）濱崎氏銅及ビ汞・耐酸性顆粒ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Verfasser wünschte die Beziehung zwischen der Störung des EiweiBstoffwechsels und den säurefesten Substanzen experimentell zu untersuchen und, weiterhin den Zusammenhang der Amyloidsubstanz mit den Zerfallprodukten der Nucleoproteide klar zu machen. Über den EinfluB der Natriumsilikatinjektion auf die Hamazakischen säurefesten Granula hat Verfasser die Resultate seiner tierexperimentellen Untersuchungen durch den Nachweis der Cr-säurefesten Granula in seiner letzten Arbeit mitgeteilt. Nämlich, durch dauernde Injektiou der Silikatlosung ergibt sich im allgemeiuen eine deutliche Verminderung der Cr-säurefesten Granula, insbesondere in der Umgebung der Amyloidsubstanz, und in der letzteren finden sich weder säurefeste Granula noch säurefeste Substanz. Verfasser hat demnach angenommen, daB die Nucleinsäure, die wichtigste säurefeste Substanz in den Abbauprodukten der Nucleoproteide, unmittelbar an der Entstehung der Amyloidsubstanz nicht beteiligt sein kann. Dabei gibt es aber noch eine Möglichkeit, daß nicht die Nucleinsäure selbst, sondern ihre weiteren Spaltprodukte (Purinderivate) eine gewisse Rolle bei der Amyloidbildung spielen konnen. Demgemäß hat sich Verfasser weiter mit dem Einfluß der Natriumsilikatinjektiou auf die Cu-säurefesten Granula, welche hauptsächlich aus Mononucleotiden und Mononucleosiden bestehen, und auf die Hg-säurefesten Granula, deren Hauptbestandteile Purinbasen sind, beschäftigt. Die Resultate dieser Untersuchung sind, zusammengefaßt, folgende: Die Cu-säurefesten, Hg-säurefesten Granula und das säurefeste Lipoid findet man nicht in der Amyloidsubstanz, welche durch die Natriumsilikatinjektion erzeugt worden ist. Die Cu-saurefesten und Hg-säurefesten Granula zeigen auch eine deutliche Verminderung und sogar oft verschwinden sie in den durch die Amyloidsubstanz infiltrierten Geweben. Jedoch hat diese Verminderung der Cu-säurefesten Granula einen inuigen Zusammenhang mit dem Ernährungszustand des Orgauismus; bei gut genährten Tieren tritt diese Erscheinung nur undeutlich zutage. Im Gegeusatz zu den Cr-säurefesten und Cu-säurefesten Granula. verringern Bich die Hg-säurefesten Granula bei einem günstigen Ernährungszustand ebenfalls sehr deutlich. Aufgrund den oben genaunten Daten kann man wohl annehmen, daß sich weder Nucleinsäure noch Purinmononucleotide bis zu Purinbasen unmittelbar an der Amyloid- bildung beteiligen konnen. Während die braunen Abnutzungspigmente bei der Verhungerung des Kontrollversuches deutlich zunehmen, findet bei der experimentellen Silikatinjektion kaum eine Vermehrung derselben statt. Die Abnutzungspigmente entstehen, wie schon Hamazaki bestätigt hat, aus den endogenen säurefesten Granula, welche Spaltungsprodukte des KerneiweiBes sind. Deshalb ist es unwahrscheinlich, daß man, wie Gierke-Aschoff es tut, den Zellkernzerfall des betreffenden Organismus als eine der wichtigsten Ursachen der Amyloiderkrankung betrachten kann. en-copyright= kn-copyright= en-aut-name=MihuneKan-ichi en-aut-sei=Mihune en-aut-mei=Kan-ichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=4 article-no= start-page=879 end-page=894 dt-received= dt-revised= dt-accepted= dt-pub-year=1938 dt-pub=19380430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die Beziechung zwischen den Hamazakischen säurefesten Granula und der durch Natriumsilikatinjektion erzeugten Amyloidosis. (I. Mitteilung) Über die Cr-saurefesten Granula kn-title=濱崎氏耐酸性顆粒ト硅曹注射ニ依ルAmyloidosisノ關係ニ就テ（第1報）「クローム」・耐酸性顆粒ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Die säurefesten Granula (Hamazaki), Abbauprodukt der Nueleoproteiae, werden in 4 Arten eingeteilt: namlich in Cr-säurefeste, Fe-säurefeste, Cu-säurefeste und Hgsaurefeste Granula. Nach Hamazaki bestehen die Cr-saurefesten Granula hauptsächelich aus Nucleinsäure und Mononucleotide. Obwohl die Entatehung der Amyloidsubstanz viel bestritten ist, doch hat die Literatur bisher kaum einheitlich ausgesagt, welche Rolle dabei das Nucleoproteid spielt. Gierke u. Aschoff legten groBen Wert auf die Zerspaltung des Kerns, und Letterer konnte durch die Nucleininjektion Amyloidablagerung hervortreten lassen. Verfasser wünschte die Beziehung zwischen den Storung des EiweiBstoffwechsels und den säurefesten Substanzen zu betrachten, und weiterhin den Zusammenhang der Amyloidsubstanz mit den Nucleoproteide klar zu machen. Demnach habe ich 2ccm 1%iger Silikatlosung pro kilo zehn Kaninchen täglich zweimal injiziert, und versohiedene Organe mittels des Forschungsverfahrens der Hamazakischen säurefesten Granula untersucht. Zur Kontrolle machte ich denselben Versuch an drei Kaninchen, welche nach 20-30 tägigem Hunger zur Untersuchung kamen. Aus der Untersuchung der einzelnen Organen ergaben sich folgende Befunde. Im Herzmuskel finden sich sparlich die Cr-säurefesten Granula, welche sich rundlich oder eckig gestalten, vor. Die Granula kommen um den Kerne herum oder in der Peripherie der Muskelfasern zum Vorschein. Hier und da, wo kein Granulum mehr vorhanden ist, farben sich die hyalinos degenerierten Muskelfasern diffus violett. In der Milz zeigen die Cr-säurefesten Granula eine deutliche Verminderung in gewohnlichen Fallen. Vor allem verschwanden sie in den Fallen, insofern die Amyloidsubstanz, in welcher man gar kein saurefestes Granulum finden kann, deutlich nachweisbar ist. In der Lunge kommt ein totales Verschwinden der Crsäurefesten Granula zustande. GroBhirn verliert die Cr-säurefesten Granula so deutlich, daB man diese in den Ganglienzellen der Rinde kaum, und feine Granula in den Pyramidenzellen des Hippocampus nur selten finden kann. Im Kleinhirn kommt eine deutliche Verminderung der Cr-säurefesten Granula zum Vorschein. Die degenerierten Purkinjeschen Zellen farben sich meist dick violett und zeigen spärliche feine Cr-säurefeste Granule. Auch in der Zunge vermindern sich zwar die Cr-saurefesten Granula im Axialteile des Zungenmuskels ziemlich, befinden sich aber noch reichlich in der Peripherie der Muskelfasern. Im Magendrusenepithel vermindern sich die Cr-säurefesten Granula mehr oder weniger. Bei der Kontrolle nehmen die saurefesten Granula dagegen auBerordentlich zu und zeigen einen deutlichen zahlenmaBigen Unterschied von den Experimentsfällen. Die Cr-säurefesten Granula der Leber haben im hohen Grade abgenommen. Vor allem vermindern sich die Granula deutlich, falls das Amyloid abgelagert ist. Die Cr-säurefesten Granula in den Harnkanalchen nehmen ihre Zahl deutlich ab und verkleinern sich auffällend. Das Zwischenstück hat die groBte Menge von Granula, dessen Zelleib sich deswegen violett färbt; dann färbt sich die auf Bowmanschen Kapseln, Harnkanalchen, und Arterienwande abgelagerte Amyloidsubstanz, welche nicht mit dem Barytwasser abgeblasen wird, diffus violett. Die dauernde Injektion der Silikatlosung ergibt, kurz gefaBt, eine deutliche Verminderung der Cr-säurefesten Granula; vor allem handelt es sich wohl bei einer ganz deutlichen Verminderung, sogar oft einem Verschwinden, um die Ablagerung von Amyloidsubstanz, iu der die säurefeste Substanz nie eingeschlossen ist. Es ist Behr wahrscheinlich, daB, je mehr die Amyloidsubstanz abgelagert ist, desto weniger die Cr-säurefesten Granula am betreffenden Tiere auftreten. Beim dauernden Hungerversuch vermindern sich die säurefeste Granula ebenfalls beträchtlich ; aber diese Verminderung tritt nicht in so starken Maße ein als bei der Silikatinjektion ; auch ist die letztere von jener durch die Verteilung der Granule leicht zu unterschieden. en-copyright= kn-copyright= en-aut-name=MihuneKan-ichi en-aut-sei=Mihune en-aut-mei=Kan-ichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=3 article-no= start-page=535 end-page=555 dt-received= dt-revised= dt-accepted= dt-pub-year=1938 dt-pub=19380331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische säurefeste Granula bei gesunden Ratten. (I. Mitteilung) Cr-säurefeste Granula. kn-title=正常白鼠ニ於ケル濱崎氏耐酸性顆粒ノ研究（第1報）「クローム耐酸性顆粒」ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hamazakische säurefeste Granula wurden bisher hauptsächlich an Kaninchen studiert. Hamazaki stellte eingehende systematische Untersuchungen über Cr-, Fe-, Cu-, and Hg-säurefeste Granula im Gewebe des gesunden Kaninchens an. Durch seine ununterbrochenen Beiträge Bind die grundlegenden Studien über die chemischen Eigenschaften der säurefesten Granula fast vollendet; das Problem über die Phylogenie sowie die Ontogenie solcher Granula steht dagegen zurzeit noch offen. Verfasser studierte an gesunden Ratten, welche als Versuchstiere neben Kaninchen am häufigsten benutzt wurden, vier Arten von säurefesten Granula. Die vorliegende 1. Mitteilung betrifft die Cr-säturefesten Granula, die von den vier Arten am interestantesten sind. Als Untersuchungsverfahren diente die Hamazakische Methode zum Nachweis säurefester Granula: Fixieren des frischen Gewebes in Müllerscher Flüssigkeit mit 5%igen Eisessig 3 Tage, Herstellung der Paraffinschnitte nach der Regel und Färben nach der Hamazakischen Karbolfuchsin-Jod-Methode. Zur Differenzierung der Granula gegen säurefestes Lipoid kam die Eraktionierungsmethode mit Barytwasser in Anwendung. Als Kontrollpräparate wurden Schnitte, die nach der Ciaccioschen oder der Regaudschen Fixierung mit Karbolfuchsin oder Ciacciosehem Sudam-III gefärbt waren, mikroskopisch untersucht. Die Resultate sind wie folgt: 1) Aus der Untersuchung der Hamazakischen Cr-säurefesten Granula in verschiedenen Organen der gesunden Ratte ergaben sich bemerkeuswerte histologische Befunde. 2) Im Vergleich zu Cr-säurefesten Granula des Kaninchens sind die der Ratte im allgemeinen grob, zum Konfluieren geneigt und unregelmässig geformt. Dies beruht wahrscheinlich darauf, dass in den betreffenden Granula Mononucleotide und Mononucleoside reichlicher als Nucleinsäure enthalten siud, und auch der Lipoidgehalt grösser ist. 3) Cr-säurefeste Granula im Gewebe bei Batten sind an Zahl bei weitem geringer als bei Kaninchen und säurefeste Lipoidgranula haben die Neigung, an ihrer Stelle in Erscheinung zu treten. 4) Von Cr-säurefesten Granula der Ratte gibt es ziemlich viele, die einen relativ starken Wiederstand gegen Barytwasser zeigen. Solche Granula schliessen reichlich adsorbiertes Lipoid in sich ein, stellen in allen Beschaffenheiten eine Übergangsform zwischen Cr-säurefesten Granula und säurefesten Lipoidgranula dar, und scheinen beim Steigern des Lipoidgehaltes in echte Lipoidgranula übergehen zu können. 5) Im Gewebe der Ratte können die Lipoidgranula nach der Fixierung der Cr-säurefesten Granula meist reichlicher und zwar auffallender als durch die Ciaccioschen Lipoidnachweismethode festgestellt werden. 6) Bei Ratten steht der Nucleinsäurestoffwechsel zum Lipoidumsatz in inniger Beziehung; im lipoidreichen Gewebe können sich beide Granula unter gewissen Bedingungen die einen in die andereu umwandeln. 7) Ebenso wie bei Kaninchen lassen sick Cr-säurefeste Granula auch bei Ratten nach der Ciaccioschen oder Regaudschen Fixiermethode in der Regel nicht fixieren, nur bei wenigen kann dies ausnahmsweise nach dem Ciaccioschen Verfahren geschehen. Nach der Meinung des Verfasser hängt die Fixierbarkeit solcher Granula vielleicht von ihrem Lipoidgehalt ab. 8) In Samenepithel erkennt man oft Cr-säurefeste Granula, die in morphologischer Hinsicht mit Mitochondria übereinstimmen. Solche Granula verschwinden durcb die Differenzierung mit Barytwasser und können im Regaudschen fixierten Material nach der Harbolfuchsin-Jod-Methode nicht dargestellt werden. Sie gehören daher zweifellos zu den echten Cr-säurefesten Granula und gelten, vom morphologischen Standpunkt aus gesehen, als Umwandlungsform der Mitochondria in gewissem Funktionszustand. 9) Durch die bisheriegen Studien konnte gar kein Verhältnis der Hamazakischen Cr-säiurefesten Granula zu Mitochondria nachgewiesen werden, dagegen konnte der Verfasser wohl ausnahmsweise im Samenepithel einen solchen Zusammenhang erkennen. 10) Bei Ratten finden sich im glatten Muskelgewebe und im Bindegewebe keine Cr-säurefeste Granula. Sie fehlen in der Regel auch in den Lymphocyten sowie in den Rindenzellen des Thymus. 11) Die Granula in histogenen Mastzellen der Ratte sind als dritte charakterische säurefeste Granule, auszusehen, welche weder zu den Cr-säurefesten Granula noch zu den säurefesten Lipoidgranula in Beziehung stehen. en-copyright= kn-copyright= en-aut-name=SugaYukio en-aut-sei=Suga en-aut-mei=Yukio kn-aut-name=菅幸雄 kn-aut-sei=菅 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=12 article-no= start-page=2551 end-page=2563 dt-received= dt-revised= dt-accepted= dt-pub-year=1939 dt-pub=19391231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die experimentelle Erzeugung der Hamazakischen säurefesten Granula und einige Beiträge über die Karyopathologie. (I. Mitteilung) Aseptische Aufbewahrung der frischen Gewebe. 2). Über die Cu- und Hg-säurefesten Granula. kn-title=濱崎氏耐酸性顆粒ノ實驗的生成竝ニ細胞核病理學知見補遺 第1篇 新鮮組織ノ無菌的保存法 其ノ2 銅・及ビ汞・耐酸性顆粒ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Verfasser wünschte den Abbauprozeb der Nucleoproteide durch die Fermente, welche in den frischen Geweben vorhanden sind, experimentell klar zu machen und hat demgemäB in seiner letzten Arbeit durch den Nachweis der Cr-säurefesten Granula über die Beziehung zwischen den Cr-säurefesten Granula und der Feulgenschen Reaktion berichtet. Diesmal untersuchte Verfasser die Cu- und Hg-säurefesten Granula unter gleichen Umständen und konnte weiterhin einige Beobachtungen über die Correlation der oben genannten 3 säurefesten Granula machen. Während die Gu-säurefesten Granula nach dem Abbauprozeb der Nucleinsäure am deutlichsten nach ca. 16-20 Stunden (22-37°C) auftreten, ergeben sich die Hg-säurefesten Granula am zahlreichsten nach ca. 20-24 Stunden (37°C) und die amorfe Hg-säurefeste Substanz vermehrt sich auch noch nach ca. 24 Stunden. Obwohl die oben genannten säurefesten Granula zum Teil an der Kernwand liegen, finden sie sich doch wohl hauptsächlich im Zelleib und zwar in dessen Peripherie. Dies ist nicht anders zu erklären als damit, daB die beiden säurefesten Granula mit dem Abbau der Nucleinsäure an derselben stelle wie die Cr-säurefesten Granula zutagetreten. Eine immer deutlicher werdende Abschwächung der Feulgenschen Reaktion trat kurz nach dem Versuche auf, trotz einer vorübergehenden Reaktionssteigerung im ganz frühen Stadium. Obwohl die Vermehrung der Cr-säurefesten Granula mehr oder weniger zeitliche Schwankungen aufwies, so kann man doch sagen, dab sie im allgemeinen ndch 8-16 Stunden (22-37°C) am deutlichsten ist. Die Cu-säurefesten Granula treten demnächst am deutlichsten nach ca. 16-20 Stunden (22-37°C) auf, dann folgen die Hg-säurefesten Granula und die säurefeste Substanz nach ca. 20-24 Stunden (37°C). Unter gleichen Bedingungen zeigen die Gu- und Hg-säurefesten Granula eine zeitliche Schwankung der experimentellen Resultate deutlicher als die Cr-säurefesten Granula. Nach den Abbaustufen der Nucleoproteide nämlich vermehren sich die 3 Arten säefester Granula in der eben beschriebenen Reihenfolge allmählich und dann vermindern sie sich mit der Zeit in derselben Reihenfolge. Die oben genannten Daten passen gut zu den Angaben Hamazkis, dab die Cr-, Cu- und Hg-säurefesten Granual jedes für sich häuptsachlich aus Purinderivaten von freier Nucleinsäure bis zu Purinbasen bestehen. Die Abschwächung der Feulgenschen Reaktion und die Vermehrung der Cr-säurefesten Granula kommen abwechselnd in inniger Beziehung zueinander vor; in solchen Geweben, welche überhaupt nur wenig mit nucleoproteidspaltenden Fermenten bedacht sind, sind diese Erscheinung nicht so deutlich. Diese Tatsache bestätigt die Meinung Hamazakis, dab das die Feulgensche Nuclealreaktion ergebende KerneiweiB nicht anderes ist als die mit Proteine verbundene Nucleinsäure, und daB die Cr-säurefesten Granula hauptpachlich aus freier Nucleinsäure bestehen. Die Abschwächung der Feulgenschen Reaktion und die Vermehrung der 3 Arten säurefester Granula zu bestimmmten Stunden stehen im Verhältnis mit der Höhe der Temperatur. Das säurefeste Lipoid, welches sich in aufbewahrten Geweben reichlich entwickelt, kann aus den lipoidhaltigen Mitochondria durch Resorption der säurefesten Substanzen entstehen, andrerseits aber auch aus den säurefesten Granula durch die Resorption des Lipoids. Die Zellkerndegeneration der aufbewahrten Geweben beginnt mit Chromatinentmischung (Hamazaki), dann geht sie durch das Stadium der Karyopyknose hindurch und endet schlieblich in Karyorrhexis. Das Stadium, in welchem die Feulgenschen Reaktion deutlich abgeschwächt erscheint und die Cr-säurefesten Granula deutlich auftreten, entsprich wohl dem Stadium des Endes der Pyknose bis zum Beginn der Karyorrhexis. In den aufbewahrten Geweben findet relativ selten ein Ubergang von der Chromatin-entmischung zur Chromatlyse und weiterhin zur Karyolyse statt, aber oft kommt unter bestimmten Bedingungen der Ubergang von der Karyopyknose zur Karyolyse vor. Obgleich die Feulgensche Reaktion in diesem Zustand , wie gewöhnlich, schwächer wird oder sogar ganz verschwindet, vermehren sich die Cr-säurefesten Granula merkwürdiger-weise nicht so deutlich. Wahrscheinlich ist dieses Phenomen durch die Wasserionen-konzentratiion den aufbewahrten Geweben bedingt. en-copyright= kn-copyright= en-aut-name=MihuneKanichi en-aut-sei=Mihune en-aut-mei=Kanichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=11 article-no= start-page=2406 end-page=2425 dt-received= dt-revised= dt-accepted= dt-pub-year=1939 dt-pub=19391130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische spezifische süurefeste Granula in der Bronchialschleimhaut der Maus. (I. Mitteilung) Über ihre histologischen Bilder bei erwachsenen sowie bei unausgewachsen gesunden Mäusen nach Anwendung der Karbolfuchsin-Jod-Methode. kn-title=二十日鼠氣管枝粘膜ニ於ケル濱崎氏特殊耐酸性顆粒ノ研究（第1報）健常成熟竝ニ幼若動物ニ於ケル之ガ組織像ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Im Jahre 1935 beschrieb Hamazaki in der Bronchialschleimhaut der Maus eine Art spezifischer säurefester Granula, die er bei seiner Hg-säurefesten Granula-Forschung zufällig gefunden hatte. Solche Granula zeigen morphologisch und biologisch sehr auffallende Eigentümlichkeiten. Sie kommen als schöne gröbere Tröpfchen an dem freien Rand der Epithelzellen zum Vorschein, und geben uns morphologisch den Eindruck von Sekretgranula. Sie sind durch andere gebräuchliche Färbungen nicht tingierbar. Es ist merkwürdig, daB man kein einziges solches Granulum bei anderen Säugetieren (wie Mensch, Affe, Kaninchen, Ratte, Meerschweinchen, Ziege und Schwein) vorfindet. Verfasser stellte systematische histochemische Untersuchungen über die spezifischen säurefesten Granula an um die Natur ihrer Grundsubstanz sowie ihre biologische Bedeutung klar zu machen. Als Versuchstiere wurden erwachsene gesundene Mäuse (11 Fälle) sowie unausgewachsene Mäuse (10 Fälle) benutzt. Frisches Lungengewebe würde mit der Hamazakischen Nachweismethode der Cr-, Cu-, Fe-und Hg-säurefesten Granula behandelt. An den mit KFJ-Methode gefärbten Schnitten wurde zum Teil Hämatoxylin-Nachfärbung ausgeführt, um den Zusammenhang der säurefesten Granula mit den betreffenden Zellkernen genau zu beobachten. Die Differenzierungsmethode durch verdünntes Barytwasser wurde dabei auch probiert. Zur Kontrollfärbung waren die Schnitte mit Hämatoxylin-Eosin oder Ciaccioscher Sudan III-Lösung gefärbt und nach der Ciaccioschen Methode Jixiertes Material mit der KFJ-Methode oder Ciaccioscher Sudan III-Lösung behandelt. Die Resultate lassen sich wie folgt zusammenfassen: 1) Bei der gesunden erwachsenen Maus sind die Hamazakischen speaifischen säurefesten Granula des Bronchialepithels immer mehr oder weniger nachweisbar, dagegen nie bei der unausgewachsenen. 2) Sie finden sicn reichtich in den Zylinderepithelzellen des Bronchiolus respiratorius (I Ordnung bis III Ordnung), aber kaum in gröBeren Bronchien, und gar nicht im kubischen respiratorischen Epithel. 3) In der Regel ergibt sich nur ein solches gröberes (3-6μ) Granulum im freien Rand bei der einzelnen Zelle, gelegentlich finden sich aber einige rundliche kleinere (1.5μ) nahe um den Kern hermn. 4) Diese Granula werden mit der Hg-Methode am deutlichsten und am reichlichsten fixiert, dann mit Cu-und Cr-, aber gar nicht mit der Fe-Methode. 5) Die Grundsubstanz der Granula ist wohl eine echte säurefeste Substanz, enthält aber eine mässige Menge von Lipoid, welches mit der Ciaccioschen Methode nicht fixierbar ist. 6) Es steht wohl auBer Zweifel, daB diese säurefesten Granula endogener Herkunft sind. Ihre Beschaffenheiten sind denen des Lipofuszins so ähnlich, daB sie mit der Hg-Methode am deutlichsten fixierbar sind, die alt gewordenen Granula eine bräunlicbe Eigenfarbe bekommen und daB ihre Zahl im allgemeinen mit dem Alter der Tiere zunimmt. 7) Aus den morphalogischen Befunden ist es wohl denkbar, daB diese Granula mit besbimmter physinlogischer Funktion als eine Art von Sekretmasse ins Bronchiallumen abgesondert werden, was bei den gewöhnlichen säurefesten Granula noch nicht nachgewiesener Ausgang ist. 8) Im mit Hg-Fixierungsgemisch gehärteten Material finden wir die Granula, welche im Begriff stehen, sich abzusondern, am deutlichsten, weniger aber bei dem mit Cu-, und nie bei dem mit Cr-Fixationsgemisch gehärteten. 9) Diese Granula werden, von den betreffenden Zellkernen produziert, und zwar die gröberen sind nichts anderes als die totale säurefeste Umwandlung des Kerns. 10) Was die kleineren betriffts,o kann man annehmen, daß sie　aus der vom Kem ausgetretenen Chromatinmasse gebildet werden, ähnlich wie der bekannte Vorgang der Kernsekretion, welcher schon van Dimitrowa, Krabbe und Berg nachgewiesen wurde. 11) Im Schleimhautepithel des Seitenbronchus und des Bronchiolus tritt auch eine ähnliche Sekretion der säurefesten Substanz aus dem Kern sowie eine sänrefest Deegeneration des Kerns zu tage, aber dieser letztere verschiebst ich weder zum Zellrand noch geht er in einen schönen Tropfen über. 12) Bei den jungen Mäusen zeigen sich die spezifischen säurefesten Granula am deutlichsten durch Cr-Fixierungsgemisch in der Schleimhaut des Bronchiolus resp., aber bei keiner der vier Fixiermethoden kann man die ringförmigen sowie blaß bräunlichen Granula, die bei erwachsenen Mäusen in Erscheinung zu treten pflegen, darstellen. en-copyright= kn-copyright= en-aut-name=MatudaKunisige en-aut-sei=Matuda en-aut-mei=Kunisige kn-aut-name=松田國重 kn-aut-sei=松田 kn-aut-mei=國重 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=11 article-no= start-page=2379 end-page=2398 dt-received= dt-revised= dt-accepted= dt-pub-year=1939 dt-pub=19391130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische säurefeste Gracula bei gesunden Ratten. (3. Mitteilung) Fe- und Cu-säurefeste Granula. kn-title=正常白鼠ニ於ケル濱崎氏耐酸性顆粒ノ研究（第3報）鐵・竝ニ銅・耐酸性顆粒ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hamazaki hat bereits auf Grund seiner chemischen Unterschungen fest9estellt, daB Cr-säurefeste Granula hauptsächlich aus freier tierischer Nucleinsäure, Fe-säurefeste Granula hauptsächlich aus Purinmononcleotiden Cu-säurefeste Granula hauptsächlich aus Purinmononucleosiden und Hg-säurefeste Granula zum gröBten Teil aus Prinbasen entsanden sind. Verfasser hat bei gesunden weiBen Ratten die oben genannten säurefesten Granula in der Hauptsaehe vom histologisehen Gesichtipunkt aus untersuehb und berichtete schon in seiner Mitteilung über die Befunde an Gr-säurefesten Granula und in seiner Zweiten Mitteilung über die an Hg-säuresesten Granula. In der vorliegenden Mitteilung handelt es sich histologische Befunde an Hamazakischen Fe- und Cu-säurefesten Granula in verschiedenen Organen und Geweben von gesunden weiBen Ratten, worüber im folgenden kurz zusammenfassend berichtet sei: 1) Fe- und Cu-säurefeste Granula haben im allgemeinen eine groBe morphologische Ahnlichkeit; jede dieser zwei Arten ist jedoch als selbständige Granula anzusehen, weil man in einem bestimmten Gewebe einen deutlichen morphologischen Unterschied zwischen ihnen finden kann. 2) Im Vergleich zu den Fe-säurefesten Granula enthalten die Cu-säurefesten Granula eine gröBere Menge Lipoid. Sie sind meistens grob und rundlich. 3) Cu-saurefeste Granula bzw. Substanzen, die von einem Harnkanälchen ausgesondert werden, sind ein Bestandteil des Harns. 4) Auf Grund des oben Gesagten stimmt Verf. der Hamazakischen Ansicht zu, daB die Gu-säurefesten Granula den Hg-säurefesten Grannula am ähnlichsten sind und deshalb als eine dem Endprodukte des Stoffwechsels sehr nahe stehende Substanz anzusehen sind. 5) Die Histiocyten in den Alveolarsepten der weiBen Ratten, anders als die in anderen Geweben, enthalten zahlreiche Cu-säurefeste Granula, sodaB sie beim Stoffwechsel der säurefesten Substanzen, eine spezifische Rolle spielen. 6) Im geschiehteten Plattenepithel sind im allgemeinen nur wenige säurefeste Substanzen vorhanden, mit Ausnahme der Cu-säurefesten Substanzen, welche in der Schleimhaut des Vordermageas in einer bestimmten Lagebeziehung zu dem Histon stehen. Die Oben genanten 4 Arten von säurefesten Granula bzw. Substanzen Hamazakis zeigen bei weiBen Ratten je nach der Art des Schwermetalls, die zur Fixstion des Gewebs dient, einen deuulichen morphologischen Unterscheid; die säurefester Lipoidgranula weisen aber, unabhängig vom Fixations verfahren, ein fast gleiohes Gewebsbild auf. Es besteht kein Zweifel darüber, daB die säurefesten Granula in verschiedenen Geweben, sowohl bei weiBell Ratten wie bei KaninChen, die säurefesten Granula von Exogen sind und demgemaB die von Eudogen produzierten säurefesten Granula äuBerst selten sind. Wenn man aber die Herkunft der säaurefesten Granula überaupt und ihre biologische Bedeutung genauer untersuchen will, so ist die Forschung der endogenen säurefesten Granula durchaus nicht entbehrlich. Gr-säurefeste Granula werden an der Schleimhaut des untern Teils des Osophagus, an der Schleimhaut des Magens sowie des Dünndarms und an den Leberzellen im Zellkerne Naehgewiesen: Fe- sowie Cu-säurefeste Granula zeigen sich ebenfalls im Zellkerne der Lymphdrüse, der Vorderfiornzelle des Rückenmarks, der Basalzelle der Zumgenschleimhaut, der Epithelzelle des vorderen Magenteils und der Leberzelle. Was die säurefesten Granula, die um den Zellkern umschrieben sind, anbetteifft, so sind sie überall zu bemerken. Wie bereits in der 2. Mitteilung berichtet wurde, wiesen die Hg-säurefesten Granula in der Drüsenzelle einen beachtenswerten spezifischen Befund auf (Cf. 2. Mitt.). Alle solche in einer bostimmten Positionsbeziehug zum Zellkerne stehenden Granula scheinen sich nicht nur als. ein Nualeinstoffwech selprodukt, sondern als eine solche Substanz zu prasensieren, die zu einem bestimten physiologischen Zweck durch die physiologiscFhuen ktione rzeugtw ird. en-copyright= kn-copyright= en-aut-name=SugaYukio en-aut-sei=Suga en-aut-mei=Yukio kn-aut-name=菅幸雄 kn-aut-sei=菅 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=9 article-no= start-page=1842 end-page=1857 dt-received= dt-revised= dt-accepted= dt-pub-year=1939 dt-pub=19390930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die experimentelle Erzeugung der Hamazakischen säaurefesten Granula und einige Beiträge über die Karyopathologie. (I. Mitteilung) Aseptische Aufbewahrung der frischen Gewebe. 1.) Beziehung zwischen Cr-sänrefesten Granula und Feulgenscher Reaktion. kn-title=濱崎氏耐酸性顆粒ノ實驗的生成竝ニ細胞核病理學知見補遺 第1篇 新鮮組織ノ無菌的保存法 其ノ1 「クローム・耐酸性顆粒」トFeulgen氏反應 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eine Art Autoferment, welches nach dem Tod des Organismus Kerneiweiß abbaut, ist schon von Salkowski, Schmaus und Albrecht u.a. experimentell nachgewiesen worden. Es spaltet Nucleoproteide und Purinkörpern in aseptisch aufbewahrten frischen Geweben. Hamazaki behauptct, daß die Cr-, Cu- und Hg-saurefesten Granula jedes für sich hauptsächlich aus Purindcrivaten von freler Nucleinsäure bis zu Purinbasen bestehen und daß das Feulgensche Nuclealreaktion ergebende Kerneiweiß nicht anderes als mit Proteinen verbundene Nucleinsäure ist. Verfasser wünschte die Beziehung zwischen den Nucleoproteidabbauprozessen durch die Fermente, welche in den frischen Geweben sind, und dem Schicksal der oben genannten 3 säurefesfesten Granula experimentell zu untersuchen. Als Material benutzte er Herz, Milz, Lymphdrüsen, Magen, Leber und Niere von gesunden Kaninchen. In der ersten Untersuchungsreihe bewahrte er die alle Organe bei 0°, 22°, und 37°C auf, fixierte sie nach 8, 16 und 24 Stunden durch das Fixationsmittlel für Cr-, Cu-und Hg-säurefeste Granula und untersuchte jedesmal die säurefesten Granula und Feulgensche Reaktion. In der zweiten experimentellen Untersuchungsreihe untersuchte er die oben angegebenen Organgewebe, welche bei 37°C aufbewahrt wurden, alle 4 bis 30 Stunden, um das zeitliche Verhältnis genauer als bei der ersten Untersuchungsreihe zu betrachten. Um die Beziehung zwischen säaurefesten Granula und Lipoid klarzustellen führte er zugleich das Ciacciosche Fixationsverfahren und Färbung aus. Die Resultate des Experiments seien hier zusammenfassend angegeben. Obgleich die Vermehrung der Cr-säaurefesten Granula mehr oder weniger zeitliche Schwankungen aufwies, so kann man doch sagen, daß sie im allgemeinen nach 8-16 Stunden (22-37°C) am deutlichsten ist, d.h. nach ungefähr 12-16 Stunden in Herz und Niere, aber nach ungefähr 8-12 Stunden in Milz, Lymphdrüsen, Magen und Leber. Die Cr-säurefesten Granula lassen sich nach der genannten Zeit im Protoplasma oder in der Umgebung des Kernes erkennen. Später vermindern sie sich allmählich in der Umgebung des Kernes und treten mehr in der Peripherie des Zelleibes auf, wo sie allmählich mit dem Lipoid, welches sich bei der Autolyse auch deutlich vermehrt, zusammenfließt, um schließlich zu säurefestem Lipoid zu werden. Letzteres färbt sich durch die Karbolfuchsinjodmethode rötlichviolett, durch das Ciacciosche Verfahren orangegelb, und zeigt gegen Differenzierung durch Barytwasser auffallenden Widerstand. Nach 20 Stunden verringert sich dieses säurefeste Lipoid allmahlich auch und ist nach ungefähr 30 Stunden kaum mehr nachzuweisen. Bei der Hämatoxylineinfarbung treten regressive Veränderungen der Zellkerne ebenfalls ungefähr nach 8-12 Stunden auf. Eine immer deutlicher werdende Abschwäachung der Feulgechen Reaktion trat kurz nach dem Versuch auf, abgesehen von einer vorübergehenden Reaktionssteigerung im ganz früheren Stadium. Zu bemerken ist dabei, daß die Reaktionsstärke mit der Vermehrung der Cr-säurefesten Granula immer herabgesetzt worden ist. Nach ungefähr 30 Stunden zeigen sich regressive Kernveränderungen sehr deutlich und die Feulgensche Reaktion fällt schon früuh negativ aus. während die Färbbarkeit durch Hämatoxylin nocch weiter abnimmt. en-copyright= kn-copyright= en-aut-name=MihuneKanichi en-aut-sei=Mihune en-aut-mei=Kanichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=10 article-no= start-page=2535 end-page=2547 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19401031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die Ketoenolgranula in verschiedenen Organen bei Encephalitis epidemica kn-title=夏期流行性腦炎屍ニ於ケル「ケトエノール顆粒」ノ研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Die phylogenetischen Studien der Ketoenolsubstanz (KES) wurden schon von Hamazaki (bei Kaninchen, Meerschweinchen, Affe, Ziege und Schwein), Suga (Ratte), Sigemori (Kröte) und Watanabe (Huhn) eingehend ausgeführt. Was die ontogenetischen Forschungen der KES anbelangt, erschienen auch Veröffentlichungen von Hiramoto hinsichtlich des Foetus von Menschen und Kaninchen. Seit langer Zeit beabsichtigten wir, die KES im gesunden sowie im pathologischen Gewebe des Menschen zu erforschen Es bot sich jetzt die erste Gelegenheit, welche uns die Untersuchungen der KES bei Encephalitis epidemica ermöglichte. Das Material wurde mit Hamazakischem Cr-und CuFixierungsgemisch gehärtet und nach der Regel wurden Paraffinschnitte hergestellt und dann nach der Karbolfuchsin-Jod-Methode (KFJ-Methode) gefärbt. Für die Darstellung des Ketoenollipoides Wurde die Differenzierungsmethode mittels Barytwassers ausgeführt. Das Alter, der Zeitablauf nach dem Tod und hauptsächliche Krankheitsveränderungen der einzelnen Fälle sind aus der Tabelle 1 ersichtlich. Aber es ist sehr bedauerlich, dass wir auf die Untersuchung des Gross-und Kleinhirns, weil sie für die nosologische Untersuchung der Encephalitis benutzt wurden, verzichten mussten. Die Ergebnisse lassen sich wie folgt zusammenfassen: 1) Verfasser untersuchten Cr-und Cu-Ketoenolgranula (KEG) bei menschlichem Material der Encephalitis epidemica im Okayama Distrikt. 2) Im allgemeinen nehmen die auf die KFJ-Methode positiv reagierenden Granula in verschiedenen Organen an Zahl und Färbbarkeit ab. 3) Wenn man aber die KFJ-Methode nach der Differenzierung mittels Barytwassers ausführt, dann nehmen die Granula zum grüssten Teil an Farbbarkeit deutlich zu. 4) Indessen ist zu bemerken, dass die feinen Granula, welche vor der Behandlung mittels Barytwassers nicht wahrnehmbar sind reichlich und deutlich vorkommen. 5) Aus den oben erwähnten Tatsachen künnen wir schliessen, dass die Granula in verschiedenen Organen der Encephalitis epidemica ausgedehnt in ergiebige fettige Degeneration geraten. 6) Die nicht verfettenden KEG sind geringiügig und finden sich vorzüglich im Innern des Kcrns oder anhaftend an der Kernmembran. 7) Bei einem Fall, welcher klinisch irrtümlich als Encephalitis epidemica diagnostiziert wurde, war die fettige Degeneration der KEG geringgradig zu finden. en-copyright= kn-copyright= en-aut-name=HamazakiYukio en-aut-sei=Hamazaki en-aut-mei=Yukio kn-aut-name=濱崎幸雄 kn-aut-sei=濱崎 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= en-aut-name=MatudaKunisige en-aut-sei=Matuda en-aut-mei=Kunisige kn-aut-name=松田國重 kn-aut-sei=松田 kn-aut-mei=國重 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 affil-num=2 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=9 article-no= start-page=2192 end-page=2202 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische spezifische säurefeste Granula in der Bronchialschleimhaut der Maus. (IV. Mitteilung.) Über ihr Verhalten beim künstlichen Pneumothorax kn-title=二十日鼠氣管枝粘膜ニ於ケル濱崎氏特殊耐酸性顆粒ノ研究（第IV報）本顆粒ノ人工氣胸ニ對スル態度ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In der letzten Mitteilung berichtete Verf. eingehend über das Verhalten der spezifischen säurefesten Granula gegen Eingriffe mit Äther und anderen verschiedenen pharmazeutischen Gasen. Die Wirkungen dieser Gase greifen dabei die Granula fast direkt an. Diesmal wurde die Untersuchung mit künstlichem, einseitigem Pneumothorax bei Mausen durchgefuhrt, um die Einflüsse auf die Granula bei den durch mechanische Schädigung verursächten Veranderungen des betreffenden Organs sowie bei den allgemeinen Veränderungen des Organismus festzuatellen. Beim Experiment wurden die Mäuse von fast gleicher Grösse (15-20g) in 2 Gruppen eingeteilt. Bei den Mausen der I. Gruppe wurde Rippenresektion ausgeführt: nämlich eine rechtsseitige Rippe wurde in der unteren Gegend der Vorderbrust ca. 3-4mm lang durchgeschnitten und mit Pinzette oder Schere wurde die Granulation jeden Tag beseitigt, um damit den Wundverschluss vermeiden zu können. Die Versuchstiere wurden 2, 4, 6, 8, 15, 22, 29, 32 und 36 Tage nach der Operation getötet. Bei den Mäusen der II. Gruppe wurde Luftinjektion in die Pleurahöhle ausgeführt: an der gleichen Stelle der Brust (rechts, vorn, unten) wie bei der Rippenresektion liess man Luft in die Pleurahöhle mit einer Spritze solange einströmen, bis der intrathorakale Druck sich mit dem atmosphärischen ausglich; nach je 3 Tagen wiederholte man die Lufteinspritzung in der gleichen Weise und tötete die Tiere 7 Tage (nach zweimaliger Lufteinspritzungen), 10 Tage (3 mal) und 13 Tage (4 mal) nach der ersten Lufteinspritzung. Ausserdem wurden der Pneumothorax von 50 Tagen Dauer auf erstere Methode und die Pneumothorax von 25 sowie 39 Tagen Dauer auf letztere Methode geprüft. Die Materialien beider Gruppen wurden nach der Hamazakischen Methode mit Cr-, Cu- und Hg-Fixierungsgemisch gehärtet und danach Paraffinschnitte hergestellt. Bei der Färbung kamen die KFJ-Methode, zum Teil Anilinwasser-Dahlia-Methode und Hämatoxylin-Eosin-Färbung zur Anwendung. Schlüsse. 1) Beim künstlichen, einseitigen Pneumothoran der Maus nehmen die spezifischen säurefesten Granula der Bronchialschleimhaut im allgemeinen an Zahl ab. Die zahlenmässige Abnahme der Granula ist bei der behandelten Brustseite viel deutlicher. 2) Beim Pneumothorax durch Rippenresektion scheint die Abnahme der Granula hochgradiger zu sein als bei dem durch Lufteinspritzung. 3) Beim künstlichen Pneumothorax nehmen die Cr-, Cu- und Hg-Granula in jedem experimentellen Fall in fast gleichem Masse miteinander an Zahl zu oder ab, dabei sieht man in dieser Beziehung keine bestimmte Ordnung wie bei der Ätherinhalation. 4) Beim kunstlichen Pneumothorax zeigen sich bei den Granula Zerfall und Schwund in mannigfaltigen Verlaufen, aber diese Veranderungen kommen relativ langsam und leichtgradig zustande. 5) Die Wiederherstellung der Granula geschieht auch relativ langsam und ein Teil der gröberen scheint von dem unmittelbaren Übergang des säurefest umgewandelten Kerns herzurühren. 6) Ein Teil der durch Pneumothorax beeinflussten Granula geht in braunes Pigment über. en-copyright= kn-copyright= en-aut-name=MatudaKunisige en-aut-sei=Matuda en-aut-mei=Kunisige kn-aut-name=松田國重 kn-aut-sei=松田 kn-aut-mei=國重 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=8 article-no= start-page=1851 end-page=1872 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische spezifische säurefeste Granula in der Bronchialschleimhaut der Maus. (III. Mitteilung.) Über ihr Verhalten gegen pharmazeutischen Eingriff kn-title=二十日鼠氣管枝粘膜ニ於ケル濱崎氏特殊耐酸性顆粒ノ研究（第III報）本顆粒ノ藥物學的刺激ニ對スル態度ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In der I Mitteilung berichtete Verfasser über die histologischen Bilder der Hamazakischen spezifischen säurefesten Granula in der Bronchialschleimhaut der Maus, und in der II Mitteilung über die karyopathologischen Forschungen; diesmal aber handelt es sich um die morphologischen Veränderungen infolge von Anwendung verschiedener pharmakologischen Substanzen. Beim Experiment teilte Verf. die Mäuse in 3 Gruppen ein. Die Maus der I Gruppe brachte er in einer bestimmten Glocke unter, und liess sie dort 10 Minuten lang Ätherdampf inhalieren. Dann tötete er sie in den verschiedenen Zeitabschnitten nach dem Erwachen. Bei der II Gruppe narkotisierte man sie in der Glocke mit Äther 10 Minuten lang. Die Narkose wurde 2 bis 5 Mal wiederholt mit je 1 stündigem Abstand, und alle Tiere wurden 40 Minuten nach dem letzten Erwachen getötet. Bei III wurde langzeitige Narkose von 25 Minuten 1 und 2 Mal versucht und die Tiere ganz gleich wie bei der II Gruppe behandelt. Als Kontrolle wurde eine 8 Minuten dauernde Chloroformnarkose 1 und 2 Mal ausgeführt, ausserdem wurden Formol-Gas-(15 und 30 Minuten) und Nitrogen-Gas-Inhalation (7 Minuten), welche ganz andersartige Gift-Gasp sind, angewandt und die Tiere je 1 Stünden nach der Inhalation getötet. Da die spezifischen säurefesten Granula, wie schon in der I Mitteilung berichtet wurde, vermuten lassen, dass es sich morphologisch um eine Art von Sekretgranula handelt, stellte Verf. Untersuchungen hinsichtlich der Wirkung des Pilokarpins und Atropins auf die Granula an. Er injizierte daher den Mausen pro 20g 0.1 sowie 0.3cc 0.1%iger Pilokarpinlosung und auch 0.01%iger Atropinlosung in der gleichen Weise und tötete sie 25 Minuten nach der Injektion. Das Lungengewebe wurde mit dem Hamazakischen Cr.-, Cu.- und Hg.-Fixierungsgemisch, ausserdem ein anderes Stück mit Alkohol, der mit Cup. sul. völlig wasserfrei gemacht wurde, gehärtet, und nach der Regel stellte man Paraffinschnitte her. Die nach der Hämazakischen Methode hergestellten Präparate waren prinzipiell nach der KFJMethode gefärbt, ausserdem wurden Hämatoxylin-Eosin, Doppelfaibung nach KFJMethode und Hämatoxylin, auch Anilinwasser-Dahlia und Ciaccios Sudan III Färbung benutzt. Die mit Alkohol fixierten waren mit Brillant Azurin B tingiert. Zuerst untersuchte Verf. eingehend die durch die Ätherinhalation verursachten verschiedenen Ver. anderungen der Granula, dann verglich er diese mit den durch andere Mittel verursachten Veranderungen der Granula, und erzielte dabei folgende Resultate: 1) Wenn man die Maus Äther inhalieren lasst, zeigen die spezifischen säurefesten Granula die Neigung zu zerfallen bzw. sich aufzulosen und nehmen an Zahl ab, um allmählich im Schwund zu geraten. 2) Der Schwund spezifischer säurefester Granula wird je nach Zunahme der Inhalationsstunde deutlicher, seine Geschwindigkeit ist an der Hg.-Granula am grössten, dann kommen die Cr.-Granula. Ebenso erfolgt ihre Wiederherstellung in umgekehrter Reihenfolge. Die Cu.-Granula scheinen in ihrem Schwund am langsamsten zu sein und ihre Wiederherstellungszeit findet zwischen denjenigen der oben genannten beiden Granula statt. 3) Durch Ätherinhalation zeigen die Granula an Form und Färbbarkeit eine bestimmte Veränderung. An den Cu.-Granula sind morphologische Variationen sehr deutlich bemerkbar: es kommt nämlich in einem Tei dieser Granula eine wabige Struktur zum Vorschein, jedoch lösen sie sich allmählich auf. Was die Färbbarkeit anbelangt, so verlieren die Hg.-Granula sie zuerst am deutlichsten, dann die Cr.-Granula, aber an der Cu.-Granula konnen wir relativ geringe Veränderungen finden. 4) Bei der Ätherinhalation treten zahlreihe staubfeine Granula in der Bronchialschleimhaut auf, was normalerweise gar nicht stattfinden, und was zweifellos von dem Zerfall spezifischer säurefester Granula herrühren dürfte. 5) Im Cu.-Präparate sind diese feinen Granula am deutlichsten, sodann im Cr.-Präparate, im Hg.-Präparate jedoch findet man sie gar nicht. Es ist besonders bemerkenswert, dass diese feinen Granula dann am deutlichsten sind, wenn die Cr.-und Cu.-Granula an Zahl am geringsten sind, und sie sich allmählich mit der Wiederherstellung spezifischer säurefester Granula vermindern. 6) Vom morphologischen und histochemischen Standpunkt aus stimmen diese feinen Granula mit den Hamazakischen typischen säurefesten Granula überein ; es ist also höchstwahrscheilich, dass sie die Hauptbestandteile der spezifischen säurefesten Granula sind. Auf Grund diesmaliger Untersuchung, bei welcher der Zerfall spezifischer säurefester Granula klar zu sehen ist, können wir nicht irrtümlich annehmen, dass die spezifischen säurefesten Granula vielleicht mehr oder weniger Lipoid enthalten. 7) Bei der Chloroforminhalation zeigen die spezifischen Granula, wenn auch nicht so deutlich, eine ähnliche Veränderung wie die bei der Ätherinhalation. 8) Bei der Formol- und Nitrogen-Gas-Inhalation nehmen die spezifischen Granula im allgemeinen an Zahl ab, zuweilen werden sogar geringe feine Granula angetroffen ; die spezifischen Granula zeigen jedoch keine systematische Umformung wie beim Äther. Hieraus können wir schliessen, dass diese Veränderungen nur durch Ortreiz der Mittel verursacht wurden. 9) Bei der Injektion des Pilokarpine und Atropins konnte man keine sichere Wirkung auf die spezifischen säurefesten Granula wahrnehmen. en-copyright= kn-copyright= en-aut-name=MatudaKunisige en-aut-sei=Matuda en-aut-mei=Kunisige kn-aut-name=松田國重 kn-aut-sei=松田 kn-aut-mei=國重 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=8 article-no= start-page=1802 end-page=1812 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die experimentelle Erzeugung der Hamazakischen säurefesten Granula und einige Beiträge über die Karyopathologie. (III. Mitteilung.) Durchtränkungsversuch des frischen Gewebes durch organische Lösungsmittel kn-title=濱崎氏耐酸性顆粒ノ實驗的生成竝ニ細胞核病理學知見補遺（第3篇）新鮮組織ノ有機溶媒浸漬實驗 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Verfasser hat die experimentelle Aufbewhrung der in Chloroform antiseptisch eingetauchten frischen Kaninchengewebe bearbeitet. Das von ihm wahrgenommene histologische Bild der säurefesten Granula ist deutlich verschieden von dem Fall, welcher einfach aseptisch im Brutofen aufbewahrt worden war. Daher unternahm er es, systematische Untersuchungen in Bezug auf diesen interessanten Befünd auszufuhren, um die Ursache dafur festzustellen. Ausser mit Chloroform beschäftigte er sich mit Tetrachlorkohlenstoff, Aether und Aether-alkohol bei 37°C, fixierte sie nach 1, 2, 4, 8, 12, 24, 48 und 72 Stunden durch das Fixationsmittel fur Cr-saurefeste Granula und untersuchte jedesmal die säurefesten Granula. Die Resultate des Experiments seien hier zusämmenfassend angegeben. Durch die Anwendung von Chloroform, Tetrachlorkohlenstoff und Aether wird das säurefeste Lipoid aus dem frischen Gewebe extrahiert, indem die echten säurefesten Granula, welche bei Aufbewahrung durch Kerndegeneration entstanden waren, im Gewebe zurückbleiben. Jedoch passt sich diese Methode nicht der histologischen Untersuchung an, weil zugleich eine Umformung der säurefesten Granula stattfindet. Bei Anwendung des Aether-Alkoholgemisches lassen sich keine echten säurefesten Granula nachweisen. Die saurefesten Granula zeigen bei dieser Aufbewahrung ein sehr merkwurdiges Bild, welches von niemand vorher wahrgenommen wurde. Sie lassen sich von Beginn an im Protoplasma als diffus zerstreute, relativ feinere, unscharf begrenzte Granula erkennen. Obwohl die Vermehrung der Cr-saurefesten Granula in allen Organen, welche mit Tetrachlorkohlenstoff aufbewahrt worden waren, mehr oder weniger zeitliche Schwankungen aufweist, so kann man doch sagen, dase sie im allgemeinen nach 1 Stund (37°C) am deutlichsten ist. Jedoch vermindern sie sich nicht so deutlich bis zu 24 Stunden, im weitern Zeitverlauf jedoch vermindern sie sich allmahlich. Die auffallenden Unterschiede zwischen dem diesmaligen Versuch und dem fruheren ergeben sich vielleicht daraus, dass infolge von Auflosung der Fettsubstanzen durch Chloroform, Tetrachlorkohlenstoff und Aether die nucleoproteolytischen Fermente vom Protoplasma aus in den Kern hinein unmittelbar eintreten konnen und dann die Crsaurefesten Granula sehr rasch in Erscheinung treten. In den aufbewahrten Geweben tritt die Karyopyknose sehr deutlich auf, aber sie geht schnell zur Chromatolyse und weiterhin zur Karyolyse ohne Aufquellung des Kerns über, was man nach der Regel der Karyopathologie als einen ungewohnlichen Verlauf ansehen kann. en-copyright= kn-copyright= en-aut-name=MihuneKanichi en-aut-sei=Mihune en-aut-mei=Kanichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=7 article-no= start-page=1635 end-page=1655 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über das Verhältnis der Hamazakischen säurefesten Granula in der anderseitigen Niere bei einseitiger Nierenarterienunterbiudung kn-title=1側腎動脈結紮ニ因ル他側腎臟ニ於ケル濱崎氏耐酸性顆粒ノ消長ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Wenn die eine Seite von Geschwisterorganen künstlich exstirpiert oder ihre Arterie unterbunden wird, so ist es wohl denkbar, dass das anderseitige Organ morphologisch und funktionell eine gewisse kompensatorische Veränderung zeigen wird. Diese Tatsache ist schon bei der Kaninchenniere von Albu, Furuya und Ozawa nachgewiesen worden. Verfasser unterband die einseitige Nierenarterie des Kaninchens, und stellte durch die Hamazakische KFJ-Methode genaue histopathologische Untersuchungen an, um die durch die Ligatur erzeugte funktionelle Veränderung der anderseitigen Niere klar darzustellen und dadurch die Ausscheidungsstelle der saurefesten Substanz leicht erkennbar zu machen. Der Stoffwechsel saurefester Substanz, welchen Hamazaki neuerdings nachgewiesen hat, ist ganz spezifisch und ihr Endprodukt wird von der Niere aus im Harn ausgeschieden. Es interessiert dabei besonders festzustellen, dass welche morphologischen Veranderungen die säurefesten Granula verschiedener Nierenteile zeigen, und gleichzeitig in welchem Verhältnis diese Granula mit den durch die Ligatur verursachten histologischen Bildern der anderseitigen Niere stehen. Als Versuchstiere wurden 18 Kaninchen von ca. 2.5Kg. Körpergewicht benutzt. Man fixierte das Tier in Rückenlage, öffnete die Bauchhohle ohne Narkose, und schloss nach Unterbindung der linksseitigen Nierenarterie die Bauchdecke wieder. Jeweils 4, 8, 12, 16, 20 und 24 Stunden nach der Operation wurden 2 Kaninchen, ausserdem 2 Tiere 10 Tage nach der Operation, durch Luftembolie getötet. Die rechtsseitige Niere war in dreifacher Weise mit dem Hamazakischen Cr-, Cu- und Hg-Fixierungsmittel gehärtet, ein anderes Stuck derselben in Alkohol, der mit Cup. sulf. völlig wasserfrei gemacht wurde, 3 Tage lang gelegt und Paraffinschnitte hergestellt worden. Die nach der Hamazakischen Methode fixierten Präparate waren mit der KFJ-Methode sowie Hämatoxylin-Eosin gefarbt, auch die Differenzierung mit Barytwasser ausgeführt. Die mit Alkohol gehärteten Präparate waren mit Brillant Azurin B gefärbt. Ausserdem wurden als Kontrollversuch noch 4 Kaninchen benutzt, die 16 und 24 Stunden nach der Laparotomie, bei welcher einfach die Baucheingeweide blossgelegt und herumgerührt waren, getötet waren. Ergebnisse: 1) Wenn die einseitige Nierenarterie unterbunden wird, so zeigt die Verteilung säurefester Granula im anderseitigen Nierengewebe eine anffallende Veranderung. 2) 4 und 8 Stunden nach der Operation nehmen die säurefesten Granula meist in den Zwischen-und Hauptstücken zu, wo man normalerweise reichliche Granula zu finden pflegt. 3) Diese Granula sitzen näher an dem freien Epithelrand als an dem Basalteil. Sie quellen auf, konfluieren miteinander und losen sich deutlich auf; eine gewisse Anzahl von ihnen nimmt seinen Weg in das Kanälchenlumen. 4) Nach 16 und 24 Stunden nehmen die Granula an dieser Stelle allmahlich ab, und dann kommen abnorme eigentümliche säurefeste Granula im Epithel des Zwischen-und Schaltstücks, Sammelröhrehens und selten auch im Epithel des Ductus papillaris zum Vorschein. 5) In diesem Stadium werden in den Epithelzellen der Haupt-und Zwischenstücke merkwürdige regressive Kerndegenerationen, nämlich Chromatinentmischung, Chromatolyse, Chromatinauslaugung und Kernhydrops, sowie Trübeschwellung des Protoplasmas nachgewiesen, was vielleicht die durch die Überbelastung der betreffenden Funktion erzeugte Zelldegeneration sein dürfte. 6) Die in den Schaltstucken und Sammelrohrchen abnorm vorkommenden säurefesten Granula sind besonders reichlich in den einzeln verstreuten speaifischen Epithelzellen zu finden. Diese Zellen sind rundlich angeschwollen, ihre Kerne bisweilen von den Granula verdeckt, und in regressive Degenerationen verwandelt. 7) Diese Zellen sind, wenn durch das Hamazakische Cr-Fixierungsmittel gehärtet, mit Hämatoxy-lin-Eosin Färbung durch ihre auffallende Acidophilität leicht unterscheidbar. 8) 10 Tage nach der Operation sind diese spezifischen Zellen noch weiter nachweisbar, aber ihre Anschwellung sowie Kerndegeneration ist nicht mehr deutlich. Ausserdem nehmen hierbei die Bilder der Haupt- und Zwischenstücke wieder ein fast nomales Aussehen an. 9) Daraus dürfte sich die Annahme ergeben, dass die Ebstein- und Nicolaierschen Uratzellen teilweise mit den oben beschriebenen angeschwollenen Zellen übereinstimmen. 10) Wie bei der normalen Niere ist das Ausscheidungsbild an Cu-säurefester Substanz ganz deutlich. 11) Aus den oben angeführten Tatsachen kann man vielleicht den Ausscheidungsvorgang an säurefester Substanz etwa folgendermassen annehmen : bewegen sich die säurefesten Granula zum Bürstenrand, dann konfluieren sie in gröberen Sekretgranula miteinander und ordnen sich wie ein Rosenkranz an. Allmählich quellen und lösen sich die Granula zur amorphen säurefesten Substanz auf, um schliesslich in das Harnkanälchen überzugehen. Normalerweise bestehen diese Sekretgranula aus Cu-und Hg-säurefester Substanz, aber es wurde bei dieser Untersuchung festgestellt, dass sie ausserdem auch Cr-säurefeste Substanz in sich einschliessen. 12) Die Stagnation der säurefesten Substanz im Nierengewebe tritt nach der Ordnung von Hg-, Cu-und Cr- Granula auf, und ihr Schwund beginnt in umgekehrter Folge. 13) Gewöhnlich finden wir im dicken Teil der Henleschen Schleife eine ziemlich grosse Anzahl der säurefesten Granula, aber sie scheinen keinen direkten Zusammenhang mit der Ausscheidung säurefester Substanz zu haben. 14) Die durch einseitige Arterienunterbindung verursachte regressive Degeneration der Kerne in den Epithelzellen der anderseitigen Niere stellt meist eine lytische Veränderung dar. Demgemäss kann man hierbei keine Zunahme der typischen, endogenen säurefesten Granula konstatieren. en-copyright= kn-copyright= en-aut-name=MatudaKunisige en-aut-sei=Matuda en-aut-mei=Kunisige kn-aut-name=松田國重 kn-aut-sei=松田 kn-aut-mei=國重 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=6 article-no= start-page=1449 end-page=1470 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über Hamazakische säurefeste Granula bei Kröten (Vufo vulgaris japonicus) (I. Mitteilung.) Über die Cr-säurefesten Granula bei Märzkröten, insbesondere über Entstehung und Verfettung der endogenen Granula. kn-title=蟾蜍 (Bufo vulgaris japonicus) ニ於ケル濱崎氏耐酸性顆粒ノ研究（第1報）三月蟾蜍「クローム」・耐酸性顆粒ニ就テ，特ニ其ノ内生的顆粒ノ産生及ビ脂肪化ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Heute steht es ausser Zweifel, dass Hamazaki's Karbolfuchsin-Jod-Methode, bei welcher eine Fixation durch Schwermetallsalzgemisch unbedingt nötig ist und als. die spezifische Nachweissmethode der Hamazakischen säurefesten Granula gilt, eine chemische Reaktion ist, und zwar eine Art von Ketonreaktion. Infolgedessen sind nun die chemischen Eigenschaften der saurefesten Granula klar geworden. Cr-säurefeste Granula bestehen hauptsächlich aus freier tierischer Nukleinsäure und auch aus Abbauprodukten, die deren Zuckerkomponente mit sich führen. Durch die unermüdlichen Forschungen Hamazaki's sind die grundlegenden Studien über die biochemischen Eigenschaften der säurefesten Granula und den Chemismus der KFJ-Methode fast vollendet, dagegen ist bis jetzt für die Forschung dieser Granula auf ontogenetischem und phyllogenetischem Gebiete noch wenig unternommen worden; die Studien über säurefeste Granula wurden bislang ausschliesslich an Mammalien, wie z.B. Kaninchen und Ratten, angestellt. Hamazaki brachte seine ausführlichen systematischen Untersuchungen der säurefesten Granula bei gesunden Kaninchen zu Ende. Er bestätigt, dass diese säurefesten Granula im Grunde etwas anderes sind als die verschiedenen schon bekannten Zellgranula und einer Art von karyogenen Granula zugehört. Konisi führte Hungerversuche bei Kaninchen durch und stellte fest, dass bei Hungern die Cr-säurefesten Granula allmählich abnehmen und in der Regel nach 5 bis 13 Tage langen Versuchen ganz verschwinden. Demnach ist es ganz sicher, dass diese durch Hunger zum verschwinden gebrachte Cr-säurefesten Granula zum Paraplasma gehören. Suga berichtete über säurefesten Granula bei normalen Ratten; bei diesen sind die Cr-säurefesten Granula im allgemeinen grob, zum Konfluieren geneigt und unregelmässig geformt sind. Er kommt daher zu der Ansicht, dass bei Ratten der Nukleinsäurestoffwechsel zum Lipoidumsatz in innigster Beziehung steht und dass sich in Lipoidreichen Geweben beide Granula unter gewissen Bedingungen die einen in die andern umwandeln können. Verfasser hatte Gelegenheit, unter der freundlichen Leitung von a.o. Prof. Hamazaki die säurefesten Granula bei Kröten zu studieren. In der I. Mitteilung berichtet Verf. uber seine eingehenden systematischen Untersuchungen der Cr-säurefesten Granula bei Märzkröten, besonders Entstehung und Verfettung ihrer endogenen Granula. Die Resultate lassen sich wie folgt zusammenfassen: 1) Bei Märzkröten steht der Stoffwechsel der säurefesten Substanz zum Lipoidumsatz noch in viel cngerer Beziehung als bei Mammalien. 2) In den Geweben der Märzkräten enthalten die Cr-säurefesten Granula eine grosse Menge Lipoid, einen Teil davon in der Form von säurefesten Lipoid umwandelt. 3) Bei Märzkröten lässt sich konstatieren, dass die reichlichen endogenen Cr-säurefesten Granula nach Austreten aus dem betreffenden Kern teilweise schnell verfetten und sogar schliesslich in säurefeste Granula übergehen. 4) In den verschiedenen Drüsenepithelien der Märzkräten bemerkt man überall mehr oder weniger endogene Granula, vor allem treten öfters alle 3 Arten von endogenen Granula in einer Zelle zusammen auf (Gehirn, Leber, Herz, Niere u.a.). Dabei beobachte Verf., dass der 3te Typus von endogenen säurefesten Granula einen relativ starkten Widerstand gegen Barytwasser und eine deutliche Affinität zu Sudan III hat. 5) Im Endstadium des Winterschlafes, wo in langerem Zeitraum keine Zufuhr von exogener säurefester Substanz stattfindet, kann man noch in den für das Leben sehr wichtigen Organen (Hirn, Herz, Leber, Niere u.a.) eine grosse Zahl Cr-säurefester Granula auffinden. 6) In den übrigen Organen und Geweben der Märzkröten ist die Farbenreaktion der Cr-säurefesten Granula im allgemeinen nicht so deutlich ; sie pflegen zu unscharfer Begrenzung zu neigen, und einen relativ starken Widerstand gegen Barytwasser zu zeigen. Dies beruht wahrscheinlich darauf, dass sich in den betreffenden Granula wasserlösliche Purinderivate relativ reichlich vorfinden und auch der Gehalt an säurefestem Lipoid grösser ist. 7) Nach Barytwasserdifferenzierung zeigen sich zuweilen die säurefesten Granula der Märzkröten ringförmig od. schaumig, und zuwar nimmt die Farbbarkeit ihrer durch Barytwasser unangriffenen Partien zu. Solche Granula hielt Verfasser für Granula, die säurefestes Lipoid sekundär an sich adsorbieren. 8) Bei Märzkröten beobachtet Verf. spärliche Abnutzungspigmente in Gehirn, Niere, und Dickdarmschlcimhaut. Diese Pigmente stammen wohl aus den Cr-säurefesten Granula, ebenso wie bei Mammalien. 9) Auch in Ovarium und Bidderschem Organ gehen die säurefesten Granula offenbar zu braunen Pigmentgranula über. 10) Bei Märzkröten beobachtete Verf. zeitweis durch Hämatoxylin metachromatisch dunkel verfärbte Granula, welche von Nukleinstoffwechselstörung der betreffenden Kerne herrühren können, in und ausserhalb der Kerne von Harnkanälchenepithelzellen sowie Lipoidzellen der Nebenniere. In solchen Zellen bemerkt man gleichzeitig ganz reichliche endogene Granula ; dies ist nicht anders zu erklären, als dass ein ganz, lnniger Zusammenhang zwischen Cr-säurefesten Granula und basophilen Granula besteht. 11) In unterem Abschnitt der Dünndarmschleimhaut finden sich eine Art von spezifischen säurefesten Granula, welche 5μ gross und nierenförmig sind, und eine starke Widerstandskraft gegen Barytwasser besitzen, ohne jedoch sudanophile Beschaffenheit zu zeigen. en-copyright= kn-copyright= en-aut-name=SigemoriHukuhitiro en-aut-sei=Sigemori en-aut-mei=Hukuhitiro kn-aut-name=重盛福七郎 kn-aut-sei=重盛 kn-aut-mei=福七郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue=5 article-no= start-page=1092 end-page=1103 dt-received= dt-revised= dt-accepted= dt-pub-year=1940 dt-pub=19400531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Granula, sowie Beiträge zur Karyopathologie. (II. Mitteilung.) Ligatur der Nierenarterie kn-title=濱崎氏耐酸性顆粒ノ實驗的生成竝ニ細胞核病理學知見補遺 第2篇 腎動脈結紮法 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Es ist lcicht verständlich, dass die Unterbindung der Art. renalis Nekrose des Nierengewebes verursacht. Anch ist schon in der Literatur darauf hingewiesen worden, dass durch autolytische Vorgange regressive Kerndegeneration bis zu Kernschwund, sowie körnigschollige Untwandlung des Protoplasmas im betreffenden Nierengewebe zustandekommen, wie dies Verf. bei aseptisch aufbewahten Geweben schon in seiner I. Mitteilung ausführlich erörtert hat. In dieser Mitteilung beschäftigt sich Verf. vom karynpathologischen Standpunkt aus mit dem Gewebstod und den Abbauprozessen der Kerneiweisse durch autolytische Fermente. Verf. hat bei erwachsenen gesunden Kaninchen linkseitige Arterienligatur des Nierenhilus vorgenommen; dann wurden die Tiere in Abständen von je vier Stunden bis 30 St. nach der Operation getötet. Darnach wurde die systernatische karyopathologische Untersuchungsniethode nach Hamazaki benutzt, d.h. Nachweis der Nucleoproteide durch Feulgensche Reaktion, das der Nucleinsäure und ihrer Abbauprodukte durch die KFJMethode, das des Histons durch Brillant A, B Färbung. Die Vermehrung der säurefesten Granula zeigt sich durch die genannte Methode im allgemeinen deutlicher als bei der aseptischen Aufbewahrung und das histologische Bild ist dort weit schöner als bei aseptischer Aufbewahrung. Ein Nachteil der Arterienunterbindung ist allerdings der Umstand, dass die histologischen Bilder der säurefesten Granula nach ihrer Lokalisation voneinander abweichen, aber wenn man einen bestimmten Herd topographisch ins Auge fast, so Jassen sich die zeitlichen Übergänge der Granula stufenweise ziemlich regelmässig verfolgen. Obwohl die Vermehrung der Cr-säurefesten Granula mehr oder weniger zeitliche Schwankungen aufweist, so kann man doch sagen, dass sie im allgemeinen nach 8-16 Stunden am deutlichsten ist. Die Cu-säurefesten Granula treten demnächst am deutlichsten nach ca. 12-20 Stunden auf, dann folgen die Hg-säurefesten Granula, und die säurefeste Substanz nach ca. 12-21 Stunden. Also, nach den Abbaustufen der Nucleoproteide vermehren sich die 3 Arten säurefester Granula in der eben beschriebenen Reihenfolge allmählich und dann vermindern sie sich mit der Zeit in derselben Reihenfolge. Die oben genannten Daten passen gut zu der Angabe Hamazaki's, dass nähmlich die Cr-, Cu- und Hg-säurefesten Granula, jede Art für sich, hauptsächlich aus Purinderivaten von freier Nucleinsaure bis zu Purinbasen bestehen. Trotz gleichen Bedingungen zeigen die Cu-und Hg-saurefesten Granula eine zeitliche Schwankung der experimentellen Resultate deutlicher als die Cr-saurefesten Granula. Das säurefeste Lipoid, welches sich bei der aseptischen Aufbewahrung reichlich entwickelt und von den lipoidhaltigen Mitochondria durch Resorption der säurefesten Substanz herrühren, ist diesmal überhaupt nirgends zu finden; es sind nur die durch Lipoidresorption verfetteten saurefesten Granula deutlich nachzuweisen. Im Gegensatz zu den säurefesten Granula trat kurz nach dem Versuche eine immer deutlicher werdende Abschwächung der Feulgenschen Reaktion auf, was man nur so erklären kann, dass fortlaufend Nucleoproteide in Purinderivate abgespaltet werden. Im Cytoplasma verhält sich die Zu-und Abnahme des Histons im allgemeinen wie die der Cr-säurefesten Granula, aber die Histonlyse (Hamazaki) tritt am deutlichsten nach ca. 16-20 Stunden auf. Es ist nicht wenig lehrreich, dass sich im Herde einer deutlichen Histonlyse zahlreiche staubfeine säurefeste Granula diffus vorfinden und so ein auffallendes histologisches Bild zu zeigen. Demgemäss müssen wir einen innigen Zusammenhang zwischen den beiden Substanzen annehmen. Bei der Histonlyse zeigt sich die Feulgensche Reaktion der Zellkerne sehr schwach; ihre Kernmembran reagiert nur mässigstark. Die Zellkerndegeneration des Nierenparenchyms beginnt mit Chromatinentmischung (Hamazaki), dann geht sie durch das Stadium der Karyopynose hindurch und endet schliesslich in Karyorrhexis. Das Stadium, in welchem die Feulgensche Reaktion deutlich abgeschwächt erscheint und die Cr-säurefesten Granula deutlich auftreten, entspricht wohl dem Stadium des Endes der Pyknose bis zum Beginn der Karyorrhexis. Obgleich die Feulgensche Reaktion in diesem Zustand, wie gewöhnlich, schwächer wird oder sogar ganz versehwindet, vermehren sich die Cr-säurefesten Granula merkwürdigerweise nicht so deutlich. en-copyright= kn-copyright= en-aut-name=MihuneKanichi en-aut-sei=Mihune en-aut-mei=Kanichi kn-aut-name=三船歡一 kn-aut-sei=三船 kn-aut-mei=歡一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=11 article-no= start-page=2322 end-page=2333 dt-received= dt-revised= dt-accepted= dt-pub-year=1941 dt-pub=19411130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranula bei Leichen von Krebskranken. II. Mitteilung. Studien bei den durch Hg-Gemish fixierten Praparaten kn-title=癌屍ニ於ケル濱崎氏「けとえのーる顆粒」ノ研究（第2報）汞・固定ニ依ル研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Der Verf., der den Vorgang des Nucleinstoffwechsels bei Leichen von Krebskranken histochemisch klarzulegen unternahm, hat in der vorhergehenden I. Mitteilung seine Studien über die Cr-Ketoenolgranula gründlich niedergelegt. Die Cr-Ketoenolgranula bestehen, chemisch betrachtet, aus den freien tierischen Nucleinsäuren und deren Spaltprodukten, welche Zucker enthalten. Im Gegensatz. dazu sind die Hg-Ketoenolgranula, die der Verf. diesmal zur Untersuchung verwendete, Endprodukte des Stoffwechsels der Ketoenolsubstanz und bestehen vorwiegend aus Purinbasen. Vor kurzem hat Hamazaki über die Hg-Ketoenolgranula im Krebsgewebe eine umfangreiche histochemische Darstellung gegeben. Der Verf. untersuchte in Sektionsfällen von Krebskranken ausführlich die Hg-Ketoenolgranula systematisch und morphologisch, und zwar nicht nur bei Krebsgeweben, sondern auch bei verschiedenen Organen des ganzen Kørpers und gelangte zu nachstehenden Schlussfolgerungen: In den verschiedenen Organen der Krebsleichen sind die Hg-Ketoenolgranula in mannigfaltiger Form anzutreffen: staubfein und rundlich, ahnähernd rundlich, eckig, schollig usw. Im Krebsgewebe kommen die Hg-Ketoenolgranula im allgemeinen in geringer Anzahl vor, jedoch sind sie, verglichen mit denselben Granula im normalen Muttergewebe, immer eher geneigt, sich zu vermehren. Im Vergleich zu den Granula bei normalen Geweben des Menschen zeigen sie in Herzmuskel, Milz, Trachea, Lunge, Kleinhirn, Zunge, Speiserøhre, Magen, Dickdarm, Pankreas, Nebennieren usw. eine Verringerung in äusserst starkem bzw. nur geringem Grade. In Grosshirn, Dünndarm, Niere usw. dagegen tritt die Verringerung in mässigem Masse auf. Solche quantitativen Schwankungen der Ketoenolgranula in den obengenannten verschiedenen Organen kann man nicht auf die vom Krebs hervorgerufenen Veränderungen allein zurückführen, da die Krebserkrankungen ausnahmslos entweder von sekundären Veränderungen oder von verschiedenen Komplikationen begleitet sind. Bei desquamierten Follikelepithelien der Schilddrüse verfallen die Kerne einer eigenartigen Kerndegeneration und erzeugen Ketoenolsubstanz. Des weiteren ist bemerkenswert: wenn krebsige Veränderungen in ausgedehntem Masse im Pfortadergebiete aufgetreten sind, so kommt bisweilen in den Leberzellen die Ketoenolsubstanz in gesteigerter Menge angehäuft zum Vorschein. en-copyright= kn-copyright= en-aut-name=KôsakaEigorô en-aut-sei=Kôsaka en-aut-mei=Eigorô kn-aut-name=高坂英五郎 kn-aut-sei=高坂 kn-aut-mei=英五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=9 article-no= start-page=1917 end-page=1931 dt-received= dt-revised= dt-accepted= dt-pub-year=1941 dt-pub=19410930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranura bei Leichen von Krebskranken. I. Mitteilung Studien bei den im Cr-Gemisch fixierten Präparaten kn-title=癌屍ニ於ケル濱崎氏「けとえのーる顆粒」ノ研究（第1報）「クローム固定」ニ依ル研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Seitdem die Ketoenolgranula von Hamazaki zum erstenmal entdeckt wurden, sind sie durch sorgfältige Untersuchungen von Hamazaki und seinen Mitarbeitern morphologisch und chemisch genau charakterisiert worden. Es stellte sich namentlich heraus, dass die Ketoenolgranula hauptsächtlich aus Nucleinsäure und Purinderivaten bestehen. Bezuglich der spezifischen Nachweismethode dieser Granula, der Karbolfuchsin-Jod-Methode, konnte man den Beweis erbringen, dass sie eine ketoenolbildende Reaktion darstellt. Hamazaki hat sich sodann in eingehenden Studien mit den einschlägigen Granula befasst und sich bemüht ihr Wesen aus Verschiedenen Vorgängen, wie z.B. Purinumsatz usw. zu erklären. In Anbetracht der Beziehungen zwischen Krebs und Purinumsatz hat er in der letzten Zeit bei operativ erhaltenen Krebsgeweben die Hg-Ketoenolgranula (säurefeste Granula) auf das genaueste untersucht. Seine Mitarbeiter, Hiramoto und Nishii, haben sogar durch die Untersuchungen der Ketoenolsubstanzen im Harn der Krebskranken zur Kenntnis der betr. Granula vieles beigetragen. Was aber die Ketoenolgranula im menschlichen Gewebe anbetrifft, so sind sie noch wenig erforscht. Hamazaki hat neulich bei normalen Geweben und dann mit Matsuda bei Encephalitis epidemica die Ketoenolgranula untersucht; Konishi sowie Nakamura haben die betr. Granula im menschlichen Skelettmuskel untersucht. Der Verf. hat nun in Sektionsfallen von Krebskranken die Cr-Ketoenolgranula im Geschwulstgewebe und in verschiedenen Organen des ganzen Körpers untersucht und erhielt folgende Ergebnisse; Im Vergleich mit den normalen Geweben des Menschen sind die Organe der Krebskranken etwas reicher an Ketoenolgranula und zwar an eckigen Granula. Ausserdem sind in diesen Organen noch rundliche bzw. grob-rundliche und hohle Granula anzutref fen, die gegen die Differenzierung mittels Barytwassers einen starken Widerstand leisten Ziemlich gross ist die Zahl dieser Granula, die namentlich Übergangsformen zu den Ketoenollipoiden darstellen. Eine hochgradige Verfettung dieser Granula jedoch, die bei der Encephalitis epidemica stattfinden kann, wird nicht beobachtet. Im Vergleich mit den normalen Menschen zeigen die Granula der Krebskranken im Herzen, in der Aorta und im Kleinhirn fast keine Veränderungen an Zahl. In der Lunge, Zunge, im Magen, Dickdarm, in der Leber, in den Nieren, Nebennieren usw. ist eine kleine Verringerung der Granula erkennbar. In der Trachea, im Grosshirn, Oesophagus, im Pankreas usw. ist die Verringerung der Granula eine massige. In der Milz und im Dünndarm dagegen tritt die Verringerung in starkerem Mässe auf. In den Krebsgeweben sind in der Regel die Granula nur in kleiner Menge vorhanden. In den älteren oder nekrotisierenden Krebsgeweben nehmen die Granula an Zahl zu. Auf Grund dieser Beobachtungen nimmt der Verf. an, dass die in der bisherigen Literatur chemisch festgestellte Vermehrung der Nucleinsäure in den Krebsgeweben hauptsachlich als eine Vermehrung der Nucleproteide oder der Chromatinsubstanz ausgedeutet werden muss. Da die Leichen der an Krebs gestorbenen Menschen entweder sekundäre Veränderungen infolge von Tumoren erleiden oder Komplikationen mit andersartigen krankheiten zeigen, ist es nicht berechtigt, die quantitativen sowie qualitativen Schwankungen der Ketoenolgranula in den obengenannten verschiedenen Organen den vom Krebs hervorgerufenen Veränderungen allein zuzuschreiben. en-copyright= kn-copyright= en-aut-name=KôsakaEigorô en-aut-sei=Kôsaka en-aut-mei=Eigorô kn-aut-name=高坂英五郎 kn-aut-sei=高坂 kn-aut-mei=英五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=8 article-no= start-page=1608 end-page=1623 dt-received= dt-revised= dt-accepted= dt-pub-year=1941 dt-pub=19410831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die Schwankungen des Gehaltes an Hamazakischen Ketoenolgranula (KEG) bei mit Viamin-A-armem Futter ernährten weissen Ratten I. Mitteilung. Über die Cr-Ketoenolgrannla (Cr-KEG) kn-title=「ヴイタミンA」缺乏食ニテ飼養サレタル白鼠ニ於ケル濱崎氏「けとえの-る顆粒」ノ消長ニ就テ（第1報）「くろ-む・けとえの-る顆粒」ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Wie allgemein bekannt, ist das Vitamin-A fettlöslich und steht zun Fettumsatz in enger Beziehung. Es erscheint dem Verf. nicht ohne Interesse, Festzustellen, wie sich die Ketoenolsubstanzen in der Form von Ketoenollipoiden (KEL) bei durch Vitamin-A-Mangel der Nahrung hervorgerufenen Stoffwechselstörungen verhalten. Verf. hab an dem Verhalten der KEG in verschiedenen Organen von weissen Ratten, die mit Vitamin-A freier Kost ernährt worden waren, Untersuchungen angestellt und berichtet in der I. Mitteilung zunächst über die Schwankungen des Cr-KEG-Gehaltes. Bei den mit Vitamin-A freier Nahrung ernährten Ratten wird in jedem Organ gewöhnlich eine Vermehrung der KEG und eine Erschöpfung, eventuell eine beträchtliche Verringerung, der KEL beobachtet. Die Vermehrung der KEG kommt wohl daher, dass einerseits die KEG, welche vorher infolge ven Verfettung physiologisch als KEL betrachtet werden konnten, arihrem Fettgehalt einbüssen und dadurch zu ihrem alten Zustand zurückkehren, und dass anderseits auch die Zellkerne degenerieren. Unter den bis heute bekanntgewordenen pathologisch-histologischen Veranderungen infolge von Vitamin-A mangel der Nahrung stehen die fortgeschrittene Verhornung des Schleimhautepithels und die Epitheldegeneration des Samenkanälchens an der Vermehrung der KEG der betreffenden Organe in unmittelbare Beziehung. Die Zunahne der KEG bei den übrigen Organgeweben kann wohl durch die infolge dem Mangels an Vitamin-A eingetretenen Stoffwechselstörungen, insbesondere durch die Fettumsatzstörung, herbeigeführt werden. Bei Mangel an Vitamin-A tritt eine beträchtliche Zunahme der KEG bei Nebennieren, Milz, Lymphdrüsen, Kleinbirn, Rückenmark, Magen und Ovarien ein. Eine ziemlich starke Vermehrung wird bei Trachea, Grosshirnrinde, Harnblase und Hypophyse beobachtet. Eine Verringerung der KEG kann man nur bei Nieren und Chorioidea wahrnehmen. Bie KEL dagegen vermehren sich nirgends. Bei Nebennieren, Lunge, Milz, Gyrus hippocampi, Chorioidea, Kleinhirn, Zunge und Grosshirn verschwinden sie sogar vollständig. Bei Lymphdrüien, Grosshirnrinde, Rückenmark, Dünndarm, Leber, Nieren, Hoden, Ovarien und Brustdrüsen bleiben sie in verschwindend geringer Menge zurück. Ferner sei erwähnt, dass die KEG der Leberzellen an der Mittelachse des Zellbalkens dichtgedrängt in grosser Anzahl vorhanden, an dessen Randpartie aber fast gar nicht anzutreffen sind. Sie sind in der engsten Beziehung zu den Stammgefässen der Gallenkapillaren bandförmig angeordnet. en-copyright= kn-copyright= en-aut-name=TakamiShuichi en-aut-sei=Takami en-aut-mei=Shuichi kn-aut-name=高見修一 kn-aut-sei=高見 kn-aut-mei=修一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=12 article-no= start-page=2037 end-page=2045 dt-received= dt-revised= dt-accepted= dt-pub-year=1942 dt-pub=19421231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Gu-Ketoenolgranula (Hamazaki) im Hunger. I. Über den Einfluss des Hungers auf die Cu-Ketoenolgranula kn-title=饑餓時ニ於ケル「銅ケトエノール顆粒」(濱崎)ノ研究（第1篇）饑餓ノ「銅ケトユノール顆粒」ニ及ボス影響ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Vor kurzem hat Konighi durch Hungerversuche bei Kaninchen die Einflüsse von Hunger auf die Hamazakischen Cr-Ketoenolgranula (Cr-KEG) untersucht und konnte die biologische Bedeutupg der Cr-KEG feststellen. Nach den ausgedehntesten histochemischen Untersuchungen. von Hamazaki bestehen Cr-KEG hauptsächlich aus den Nucleinsauren, Fe-und Cr-KEG aus abbauprodukten der letzteren, Hg-KEG bestehen hauptsächlich aus den Purinbasen. Alle diese Granula bilden unter Beteiligung der Lipoide ein spezielles Stoffwechselsystem. Für die Feststellung biologischer Bedeutung der Ketoenolsubstanzen ist die Untersuchung der Cr-KEG, der Substanzen ersten Ranges im genannten Stoffwechselsystem, ohne Zweifel von Wichtigkeit; wenn man aber das Gesamtbild diesen Systems ins Tageslicht bringen wollte, darf man ausführliche Untersuchungen auch niedrigerer Spaltprodukte nicht vernaehlässigen. Darum hat der Verf. unter Leitung von a.o. Prof. Dr. Hamazaki Hungerversuche bei Kaninchen ausgeführt, um den Einfluss des Hungers auf die Cu-KEG festzustellen. Die Ergebnisse wareh wie folgt: 1) Der Einfluss von Hunger weist bei Cu-KEG von Kaninchen viel stärkere Schwankungen auf als bei Cr-KEG. 2) Cu-KEG sind Granula, die ins Paraplasma eingereiht werden müssen, da, sie im Hunger gleich Cr-KEG versch winden der Cu-KEG fin det in den lymphadenoide Geweben einschliesslich def Thymusdrüse am erheblichsten statt. 4) Cu-KEG verschwinden im Hunger in den meisten Fällen etwas schneller als Cr-KEG. 5) In der Tatsache, dass bei Gu-KEG nach 5 tägigem Hunger das Versch winden bezw. die Verringerung am stärksten stattfndet, sind die Cu-KEG im allgemeinen den Cr-KEG sehr ähnlich. 6) Die Ketoenollipoide stimmen mit denen der Cr-Fixation darin überein, dass sie im Hunger quantitativ nicht abnehmen, abgesehen davon, dass sie sich im Anfang des Hungers bisweilen einigermassen verringern. 7) Die Cu-Ketoenolsubstanzen, die frei ausserhalb der Zellen vorhanden sind, vermehren sich im Hunger etiwas stärker als bei normalen Zeit. en-copyright= kn-copyright= en-aut-name=WatanabeNaoaki en-aut-sei=Watanabe en-aut-mei=Naoaki kn-aut-name=渡邊直明 kn-aut-sei=渡邊 kn-aut-mei=直明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=10 article-no= start-page=1777 end-page=1791 dt-received= dt-revised= dt-accepted= dt-pub-year=1942 dt-pub=19421031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranura bei Leichen von Sarkomkranken kn-title=肉腫屍ニ於ケル濱崎氏「ケトエノール顆粒」ノ研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Vorher hat der Verf. an den Leichen von Krebskranken die Hamazakischen Ketoenolgranula (KEG) eingehender untersucht und über die Ergebnisse ausführlich berichtet. Diesmal hat er bei Leichen von an Sarkom gestorbenen Kranken die KEG in den verschiedenen Organen erforscht und kam zu folgenden Eegebnissen: In den verschiedenen Organen und Sarkomgeweben der an Sarkom gestorbenen Menschen wurde weitgehende fettige Entartung der Cr- und Hg-KEG beobachtet. Diese fettige Entartung ging in den Meisten Fällen mit einer Vergrosserung und Umformung der KEG einher. Durch Differenzierung mittels Barytwassers wurden die KEG in der Farbreaktion erheblich gesteigert und zeigten bisweilen auch eine gewisse Vermehrung der feinen Granula. In der letzten Zeit haben Hamazaki und Matsuda über fettige Dageneration der KEG bei den der Encephalitis epidemica erlegenen Leichen berichtet Nach den Verf. erleiden dabei die KEG keine Veränderung in ihrer ürsprunglichen Form. Bei den Sarkom-Leichen trat die Verfettung der KEG in der Leber, Niere, Nebenniere und im Sarkomgewebe in sehr erheblichem Masse aus, während sie in den übrigen Organen nur in geringem Masse beobachtet wurde. Es scheint jedoch nicht ganz berechtigt zu sein, die erwöhnten Befunde vollständig dem Einfluss des Sarkoms zuzuschreiben, weil an den untersuchten Sarkom-Leichen auch noch verschiedene Komplikationen oder sekundäre Veränderungen festgestellt wurden. Ferner ist zu bemerken, dass das Gewebe des Rhabdomyom im Vergleich zu andersartigen Sarkomen an Ketoenolsubstanzen viel reichlichen war und dass beim Vorhandensein irgendeiner Geschwulst im Pfortadergebiet die KEG in der Leber reichlich angehäuft vorkamen. en-copyright= kn-copyright= en-aut-name=KosakaEigoro en-aut-sei=Kosaka en-aut-mei=Eigoro kn-aut-name=高坂英五郎 kn-aut-sei=高坂 kn-aut-mei=英五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=4 article-no= start-page=618 end-page=628 dt-received= dt-revised= dt-accepted= dt-pub-year=1942 dt-pub=19420430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Über die quantitativen Schwankungen der Hamazakischen Ketoenolgranula (KEG) bei mit Vitamin-A freiem Futter ernährten weissen Ratten II. Mitteilung Über Hg- und Cu-Ketoenolgranula (Hg- u. Cu-KEG) kn-title=「ヴイタミンA」缺乏食ニテ飼養サレタル白鼠ニ於ケル濱崎氏「けとえのーる顆粒」(耐酸性顆粒)ノ消長ニ就テ（第2報）汞竝ニ銅「けとえのーる顆粒」ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In der vorhergehenden I. Mitteilung hat Verf. bei mit Vitamin-A freiem Futter ernährten Ratten das Verhalten der Cr-KEG untersucht und darüber Interessantes berichtet. Für die Erforschung der KEG ist abes die Untersuchung der Cr-KEG allein noch nicht vollauf hinreichend. Von den 4 Arten von KEG muss man, will man sich hinlängliche Kenntnisse vom Verhalten der KEG in den einzelnen Organen erwerben, wenigstens 3 Arten untersuchen, unter denen die im höchsten Range stehenden Cr- bezw. die dem niedrigsten Range zugehörigen Hg-KEG vertreten sein müssen. Darum will Verf hier nach dem vorangehenden Bericht über Cr-KEG den Bericht über seine Versuchsergebnisse bezüglich der Cu- und Hg-KEG geben. In den einzelnen Organen von mit Vitamin-A freier Kost ernährten weissen Ratten nehmen die mercuraffinen Substanzen im allgemeinen ab, was in den Herz- und-Skelettmuskeln in erheblichem Masse der Fall ist. Dabei verringern sich in verschiedenen Organen die Hg-KEG; nur in der Milz und Lunge, im Plexus chorioidei und im Dickdarm weisen sie eine quantitative Zunahme auf. Die Cu-KEG nehmen in der Regel an Menge ab, und zwar ist die Abnahme in Lunge. zunge, Leber und Nieren auffallend stark, in der Milz, in den Skelettmuskeln und im Pankreas ziemlich stark; in keinem der Organe wird irgendeine Zunahme der Granula beobachtet. Den Grund, warum die Hg- und Cu-KEG bei den mit Vitamin-A freier Nahrung ernahrten Ratten quantitativ abnehmen, während die Cr-KEG im allgemeinen zunehmen, muss man wohl einzig und allein in der infoige von Darniederliegen der Lebenskräfte der Tiere eintretenden Herabsetzung Vorgänge des oxydativen Abbaues suchen. Bei Anwendnng der Hg-fixation vermehren sich die Ketoenollipoide (KEL) in den meisten Organen, wahrend sie sich bei Anwendung der Cu-Fixation erheblich verringern. Die Ursache dieser gegensätzlichen Erscheinungen ist zwar noch nicht sicher festgestellt, es ist aber von Interesse fur uns, dass die KEL je nach der Verschiedenheit der Fixationsart hinsichtlich ihrer biologischen Bedeutung völlig verschieden in Erscheinung treten. Der auffälligste Befuud an den Hg-KEG ist die Erscheinung eigenartiger Granula. In jedem der Zellkerne sind eines oder einige davon aufgespeichert. Durch die Differen-zierung mittels Barytwassers treten sie in einem dunkelvioletten bezw. schwarzen Farbton als kleinschollige oder rundliche Granula sehr deutlich zutage. Diese eigenartigen Granula darf man wohl in den Bereich der Granula einreihen, die Hamazaki voreinigen Jahren im Zellkern der Krebsgewebe nachgewiesen hat. Sie lassen sich in zwei Arten einteilen: die eine färbt sich vor der Differenzierung in Barytwasser bereits hellviolett, während die andere erst nach der Differenzierung in Barytwasser in die Erscheinung tritt. en-copyright= kn-copyright= en-aut-name=TakamiShuichi en-aut-sei=Takami en-aut-mei=Shuichi kn-aut-name=高見修一 kn-aut-sei=高見 kn-aut-mei=修一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=8 article-no= start-page=1101 end-page=1111 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut. V. Mitteilung Studien beim Menschen kn-title=嗅球及ビ嗅粘膜ニ於ケル濱崎氏Ketoenol顆粒ノ研究（第5報）人體ニ就キテ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Der Verf., der vorher bei Kaninchen, Meerschweinchen, weissen Ratten und Mäusen die Hamazakischen Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut untersuchte und daruber ausfuhrlich berichtete, hat diesmal im Anschluss an diese Untersuchungen Studien und Beschreibung derselben Granula bei den zur pathologischen Obduktion eingelieferten menschlichen Leichen vorgenommen, um schliesslich die Versuchsergebnisse beim menschlichen Material und den oben genannten Tieren zusammenfassend miteinander zu vergleichen. Als Material dienten die Leichen erwachsener Menschen, an denen seit dem vorletzten Jahre in unserem Institut eine pathologische Sektion ausgeführt worden war. Von diesen Leichen wählte der Verf. besonders 30 Fälle aus, in denen postmortale Veränderungen noch möglichst leichtgradig vorzufinden und keine Affektionen im Bereich der Nase festzustellen waren. Die Ergebnisse lassen sich folgendermassen zusammenfassen: 1. Die Ketoenolgranula im menschlichen Bulbus olfactorius treten durch CrFixierung am reichlichsten auf, weniger reichlich durch Fe-Fixierung. Sie verhalten sich also etwas anders als bei den genannten Tieren, bei denen sie durch Fe-Fixierung am reichlichsten auftraten. 2. Die menschliche Riechschleimhaut ist hinsichtlich des Gehaltes au Ketoenolgranula etwas ärmer als die der Kaninchen; sie treten aber gleich wie bei Kaninchen durch Fe-Fixierung am reichlichsten in Erscheinung. 3. In der Nasenschleimhaut der Regio respiratoria finden sich die Ketoenolgranula im Vergleich zu denen der Regio olfactoria in kleinerer Menge. Ihre Begrenzung ist viel versch wommener als bei den Tieren. 4. Die Ketoenollipoide lassen sich im Bulbus olfactorius im Gu-Gemisch am reichlichsten fixieren, weniger reichlich im Cr-, Hg- und Fe-Gemisch in dieser Reihenfolge. In der Begel sind sie in etwas grösserer Menge anzutreffen als bei den Tieren. In der Nasenschleimhaut finden sie sich im allgemeinen nur spärlich. 5. Die in den Nervenzellen des Bulbus olfactorius reichlich anzutreffenden Granula sind meistens grob, auf einer Seite der einzelnen Zelle dicht gedrängt vorhanden und stark geneigt, miteinander zu verschmelzen. Die Ketoenolsubstanzen in der Markscheide der Nervenfasern verlieren zum Unterschiede von denen der Tiere durch Differenzierung mittels Barytwassers fast vollständig ihre Färbbarkeit. 6. Aus alledem geht hervor, dass die Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut meistens in geringerer Menge beim Menschen als bei den Tieren anzutreffen sind. Dies röhrt wohl davon her, dass der Geruchssinn bei Menschen und Tieren in der Entwicklung tiefgreifende Unterschiede aufzuweisen hat. en-copyright= kn-copyright= en-aut-name=WatanabeTakeshi en-aut-sei=Watanabe en-aut-mei=Takeshi kn-aut-name=渡邊武 kn-aut-sei=渡邊 kn-aut-mei=武 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=7 article-no= start-page=987 end-page=994 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut. IV. Mitteilung, Studien bei Mäusen kn-title=嗅球及ビ嗅粘膜ニ於ケル濱崎氏Ketoenol顆粒ノ研究（第4報）二十日鼠ニ就キテ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Der Verfasser, der in den letzten 3 Arbeiten die Hamazakischen Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut des normalen Kaninchens, Meeerschweinchens und der normalen Ratte erforscht hatte, berichtet diesmal uber die folgenden Untersuchungsresultate der genannten Granula bei der normalen Maus. 1) Im Bulbus olfactorius der Maus zeigen sich Fe-Ketoenolgranula wie bei Kaninchen und Meersch weinchen am deutlichsten und reichlichsten; sie nehmen an Zahl in der Reihenfolge von Cr-, Cu- und Hg-granula ab. 2) In der Riechschleimhaut treten die Ketoenolgranula bei den durch Gr-und HgGemisch fixierten Praeparaten reichlich in die Erscheinung, die jedoch geringer sind und sich unregelmässiger als beim Bulbus olfactorius gestalten. Sie sind geringer als die des Kaninchens, aber fast gleich an Zahl mit denen des Meersch weinchens und der Ratte. 3) In der Riechschleimhaut sind die Ketoenolgranula geringer und undeutlicher als beim Kaninchen, Meersch weinchen etc. 4) Die Ketoenolgranula im Jacobsonschen Organ der Maus sind etwas reichlicher und deutlicher als die des Kaninchens etc. 5) Die Ketoenollipoide sind reichlich bei den durch Cr-Gemisch fixierten Praeparaten, denen die durch Cr-Gemisch fixierten folgen. 6) Es gibt einen besonderen Befund in den Riechdrüsenzellen, wo sich eine grosse Menge von rötlich violetter und grosscholliger Ketoenolsubstanz bei den durch Cr-, Cuund Hg-Gemisch fixierten Praeparaten zeigt. Diese Substanz äussert sich als diffuse Färbung der Zellkerne od. deckt die Kerne uber od. haftet an der Kernmembran an od. fullt sich im Zelleib miteinander einschmelzend. 7) An den Riechnervenfasern der Maus treten Ketoenolgranula reichlichor als bei den oben beschriebenen Tieren auf. en-copyright= kn-copyright= en-aut-name=WatanabeTakeshi en-aut-sei=Watanabe en-aut-mei=Takeshi kn-aut-name=渡邊武 kn-aut-sei=渡邊 kn-aut-mei=武 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=7 article-no= start-page=995 end-page=1005 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazaki'schen Ketoenolgranula bei Carassius auratus. II. Mitteilung. Untersuchung durch Fe-Fixation kn-title=鮒 (Carassius Auratus) ニ於ケル濱崎「ケトエノール顆粒」ノ研究 第2報 鐵固定ニ依ル檢索 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Im Ansehluss an die vorhergehende I. Mitteilung hat der Verf. diesmal durch Anwendung der Fixation mittels Fe-Gemisches die Ketoenolgranula der Karausche untersueht. Bei der Karausche treten die Fe-Ketoenolgranula, im Vergleich zu den Cr-Ketoenolgranula in viel grösserer Menge auf, sind scharf abgesetzt und enthalten keine bläschenartigen Granula vermischt. Ebenso wie die Cr-Ketoenolgranula sind sie auch arm an Gehalt von Lipoiden. Sie sind von eckiger oder unregelmässig rundlicher Form, im allgemeinen regelmässig fein. Winter und Sommer rufen bei den Fe-Ketoenolgranula zahlenmäBig keinen grossen Unterschied in vielen Organen hervor. Im Hirnmark, im Mesonephros, im Hoden usw. nehmen sie aber im winter an Zahl wenn auch in geringfugigerem Masse als im Sommer zu. In der Leber hingegen zeigen sie die Tendenz, im Sommer etwas mehr als im Winter zuzunehmen. en-copyright= kn-copyright= en-aut-name=TakasakaEigorô en-aut-sei=Takasaka en-aut-mei=Eigorô kn-aut-name=高坂英五郎 kn-aut-sei=高坂 kn-aut-mei=英五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=5 article-no= start-page=706 end-page=718 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranula bei Carassius auratus. (I. Mitteilung) Über die Cr-Fixation kn-title=鮒 (Carassius Auratus) ニ於ケル濱崎「ケトエノール顆粒」ノ研究（第1報）「クローム」固定ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Als Versuchstiere dienten insgesamt 38 männliche sowie weibliche Karauschen (Carassiusauratus) von verschiedener Grösse, welche im Winter Anfang Februar und im Sommer Anfang Juli in der Näbe der Stadt Okayama gefangen wurden. Bei 22 von diesen Karauschen wurde dann die Cr-Fixation ausgeführt, um an verschiedenen Organen dieser Fische die Hamazaki'schen Ketoenolgranula zu untersuchen. Die Cr-Ketoenolgranula verschiedener Organe der Karausche sind meistens rundlich oder unregelmässig rundlich gestaltet, weisen mehr oder weniger adhärente Beschaffenheit auf, enthalten Ketoenollipoide in nur verhältnismässig kleiner Menge und sind darum wenig resistent gegen Barytwasser-Differenzierung. Im Sommer zeigen die Cr-Ketoenolgranula der Leber, Milz, des Hodens und Darmkanals eine etwas grössere zahlenmäBige Zunahme als im Winter, Die Tatsache, dass die ebengenannten Organe für die Erhaltung der Individuen sowie der Art sämtlich von unentbehrlicher Wichtigkeit sind, legt den Schluss nahe, dass anch bei Karauschen die Cr-Ketoenolgranula biologisch eine sehr wichtige Rolle spielen. en-copyright= kn-copyright= en-aut-name=KôsakaEigorô en-aut-sei=Kôsaka en-aut-mei=Eigorô kn-aut-name=高坂英五郎 kn-aut-sei=高坂 kn-aut-mei=英五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=5 article-no= start-page=697 end-page=705 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studien über die Hamazakischen Ketoenolgranula im Bulbus olfactorius und in der Riechschleimhaut. III. Mitteilung. Studien bei Ratten kn-title=嗅球及ビ嗅粘膜ニ於ケル濱崎氏Ketoenol顆粒ノ研究（第3報）白鼠ニ就キテ en-subtitle= kn-subtitle= en-abstract= kn-abstract=Der Verf., der über die Untersuchungsergebnisse der Hamazakischen Ketoenolgranula im Bubus olfactorius und in der Riechschleimhaut des normalen Kaninchens und Meerschweinchens berichtet hatte, erforschte diesmal die genannten Granula bei der normalen Ratte. Es ergaben sich folgende interessante Resultate: 1) Im Bulbus olfactorius der Ratte sind Cu-Ketoenolgranula am deutlichsten und reichlichsten anzutreffen. 2) Die Ketoenolgranula in der Riechschleimhaut zeigen sich bei den durch Fe-Gemisch fixierten Praparaten am reichlichsten, dagegen bei den durch die anderen drei Gemische fixierten fast gleichartig spärlich. 3) In der respiratorischen Nasenschleimhaut sind Cr-Ketoenolgranula relativ reichlich vorhanden, denen dann Hg-Granula folgen. 4) Die Ketoenolgranula im Jacobsonschen Organ sind uberhaupt spärlich, aber bei den durch Fe- und Hg-Gemisch fixierten Präparaten sind sie relativ zahlreich. Diese Granula haben im allgemeinen unregelmässige und undeutliche Gestalt wie beim Kaninchen und Meerschweinchen. 5) Die Ketoenollipoide treten bei den durch Cr-Gemisch fixierten Präparaten relativ reichlich, im Gegensatz zu den bei Kaninchen und Meerschweinchen, in Erscheinung. 6) Die Cr-Ketoenolgranula in den Mitralzellen des Bulbus olfactorius sind meistens parallel zur Verlaufsrichtung der Neurofibrjllen im Wurzelabschnitt des Nervenfortsatzes gelagert. Sie zeigen sich hüllenatig od. granulaarrtig wie die Ketoenolsubstanz in den Nervenfasern. 7) Im Riechdrüsenepithel findet man bei den durch Hg-Gemisch fixierten Praparaten einen merkwürdigen Befund: im Basalteil der Drüsenzellen ist der Zelleib mit Metachondriagranula ausgefüllt, welche leicht violett und matt zum Vorschein kommen, sodann zeigt der Kern als helles Feld. Hie und da ist die Ketoenolsubstanz im Basalteil der übrigen Riechdrüsenzellen als Käppchen anzutreffen, welche sich dreieckig gestalten und mit ihrer Basis an der Kernmembrane anhaften. en-copyright= kn-copyright= en-aut-name=WatanabeTakeshi en-aut-sei=Watanabe en-aut-mei=Takeshi kn-aut-name=渡邊武 kn-aut-sei=渡邊 kn-aut-mei=武 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=85 end-page=93 dt-received= dt-revised= dt-accepted= dt-pub-year=1943 dt-pub=19430131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=tudien über die Hamazakischen Cu-Ketoenolgranula im Hungerzustand. II. Über den Einfluss der Nucleinsäure auf die Cu-Ketoenolgranula bei ausgehungerten Kaninchen kn-title=饑餓時ニ於ケル「銅ケトエノール顆粒」（濱崎）ノ研究（第2篇）核酸給與ノ饑餓家兎「銅ケトエノール顆粒」ニ及ボス影響ニ就テ en-subtitle= kn-subtitle= en-abstract= kn-abstract=In der letzten Zeit hat Konishi bei ausgehungerten Kaninchen festgestellt, dass verringerte oder verschwundene Cr-Ketoenolgranula (Cr-KEG) durch Einverleibiang von Nucleinsäure in erheblichem Masse wieder in die Erscheinung treten, woraus er geschlossen hat, dass diese Granule von der Nucleinsäure und ihren Spaltungsprodukten abstammen. Der Vert hat nun bei Hungerkaninchen den Einfluss ein verleibter Nucleinsäure auf die Cu-KEG untersucht. Die Methode bestand darin, dass 5 Tage lang ausgehungerten Kaninchen Wasserlösung des Nucleinsäuren Natriums intravenös injiziert oder such peroral verabreicht wurde. Als Kontrolle dienten die Kaninchen, denen Traubenzuckerlösung oder physiologische Kochsalziösung intravenös injiziert. worden war. Die Ergebnisse waren wie folgt: 1. Die Einflüsse der Nucleinsäureverabreichung auf die Cu-KEG des verhungerten Kaninchens werden in etwas starkere quaptitative Schwankungen versetzt als die Cr-KEG. 2. Durch intravenose Injektion der Nucleinsäurelösung werden bei ausgehungerten Tieren die abgenommenen oder verschwundenen Cu-KEG zwar wiederhergestellt, im Grad der Wiederherstellung aber steht dieser Fall gegen den der Cr-KEG etwas zuruck. 3. Durch perorale Darreichung derselben Losung werden bei Hungertierep die abgenommenen oder verschwundenen Cu-KEG wiederhergestellt. 4. Durch perorale Darreichung erweist sich die Nucleinsäurelösung, dass sie die Cu-KEG im Vergleich zu den Cr-KEG in stürkerem Masse wiederherstellt bezw. zur Vormehrung bringt. 5. Die Zunahme der Granula findet in der Regel in den an Granula stets reichen Organen, wie Grosshirn, Herz, Zunge, Niere usw., statt. 6. Durch die Injektion von Glykose werden die Cu-KEG zu einer gewissen Vermehrung gebracht. en-copyright= kn-copyright= en-aut-name=WatahabeNaoaki en-aut-sei=Watahabe en-aut-mei=Naoaki kn-aut-name=渡邊直明 kn-aut-sei=渡邊 kn-aut-mei=直明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山醫科大學病理學教室 END start-ver=1.4 cd-journal=joma no-vol=69 cd-vols= no-issue=3 article-no= start-page=855 end-page=860 dt-received= dt-revised= dt-accepted= dt-pub-year=1957 dt-pub=19570331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Biological Reaction to a Dye Protein I Congo red-azo-serum protein and its serological specificities kn-title=色素蛋白に対する生体の反応 第一編 コンゴーロートを用いて作つた色素蛋白及び之を用いての血清学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The hydrophilic dye-proteins have been prepared combining oxen serum protein with Congo red (CR) in varied proportion. DPO No.1 (50 mg. of CR per 100 cc. of serum), 2 (100 mg. per 100 cc), 3 (500 mg per 100 cc), 4 (1500 mg. per 100 cc). The binding between protein and dye molecules proved to be fairly strong by the test using several protein-precipitating agents and electrochromatograph. 1) The paraenteral introduction of these dye-proteins proved to produce two sorts of antibodies, the antibodies to the normal serum protein and to the dye-protein. The antibody to the normal serum decreased as the quantity of dye bound to antigen increased, showing the increased production of the antibody specific to the dye-protein. 2) Antibody formation varied according to the varied root of the introduction of antigen. Subcutaneous and intraperitoneal injection resulted in the higher antigen production comparing to the intravenous injection. The dye-protein introduced were mainly found in the macrophages. 3) The sensitized animals showed a severe shock symptomes by the reinjection of antigen. From the results mentioned above it has been suggested that the responsibility for the antibody formation to the dye-protein will be attributed to the macrophages, in which cytoplasm the injected antigen is found in a mass. en-copyright= kn-copyright= en-aut-name=KamonShunzo en-aut-sei=Kamon en-aut-mei=Shunzo kn-aut-name=掃部俊造 kn-aut-sei=掃部 kn-aut-mei=俊造 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部病理学教室 END start-ver=1.4 cd-journal=joma no-vol=17 cd-vols= no-issue=1 article-no= start-page=1 end-page=5 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20101224 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=西南日本の熱変成超苦鉄質岩体中のかんらん石と輝石の組成変化に関する覚書 kn-title=A note on compositional variation of olivine and pyroxene in thermally metamorphosed ultramafic complexes from SW Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=This short article presents some diagrams showing the compositional variations of primary and metamorphic olivine, orthopyroxene and clinopyroxene in peridotites and serpentinites from thermally metamorphosed ultramafic complexes in SW Japan. In contrast to olivine, which shows a gradual change of chemical composition corresponding with metamorphic grade, orthopyroxene and clinopyroxene show clear differences in composition between primary and metamorphic phases. Compared with primary pyroxenes, even though their compositions could be variable depending on original rock composition, metamorphic orthopyroxene and metamorphic clinopyroxene is clearly deficient in Cr(2)O(3) and CaO, and in Cr(2)O(3) and Al(2)O(3), respectively. These characteristics are useful for the discrimination between the pyroxenes of different origin. en-copyright= kn-copyright= en-aut-name=NozakaToshio en-aut-sei=Nozaka en-aut-mei=Toshio kn-aut-name=野坂俊夫 kn-aut-sei=野坂 kn-aut-mei=俊夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Earth Sciences, Faculty of Science, Okayama University en-keyword=chemical composition kn-keyword=chemical composition en-keyword=olivine kn-keyword=olivine en-keyword=orthopyroxene kn-keyword=orthopyroxene en-keyword=clinopyroxene kn-keyword=clinopyroxene en-keyword=metaperidotite kn-keyword=metaperidotite END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=9-1 article-no= start-page=5695 end-page=5704 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Histochemical Studies on the Degeneration of Retina Treated with Sodium Iodate Report II. On Histopathological Changes and HAMAZAKI'S Hg-Keto Enol Substance kn-title=沃度酸曹達による網膜変性症の組織化学的研究 第2報 クロームケトエノール物質並びにD. N. A. の変化に就て en-subtitle= kn-subtitle= en-abstract= kn-abstract=The author studied on the early histochemical changes of the peculiar retinal degeneration of the rabbit treated with intravenous injection of 5% solution of sodium iodate as the preceding report. The Cr-K E S (chrmo-keto-enol substance of HAMAZAKI), chief constituents of which are nucleotides of D N A, in the pigment epithelium and outer segments of rods begins to diminish gradually after 12 hrs. D N A decreases as the pigment epithelium and visual cells degenerate. In general, the Cr-K E S diminishes and disappears earlier than the Hg-K E S in the pigment epithelium and in visual cells. The authers that sodium iodate acts rather indirectly on the metabolism of the D N A and related substances in the outer layers of the retina. en-copyright= kn-copyright= en-aut-name=KoyamaYasuta en-aut-sei=Koyama en-aut-mei=Yasuta kn-aut-name=小山泰太 kn-aut-sei=小山 kn-aut-mei=泰太 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部眼科学教室 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=8-2 article-no= start-page=5265 end-page=5270 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590815 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Exprimental and Clinical Study in the Effects, on External Secretion of the Liver and Pancreas and Digestion and Abs orption, of Cutting Automonic Nerves in Radical Operation of Stomach Cancer with Complete Removal ol the Regional Lymphnodes Part 3. Clinical Study in the Effects on Digestion and Absorption of Cutting Autonomic Nerves in Radical Operation of Stomach Cancer with Complete Removal of the Regional Lymphnodes kn-title=根治的胃癌リンパ節廓清手術時における自律神経切断の肝，膵外分泌および消化吸収能におよぼす影響に関する実験的ならびに臨床的研究 第3編 根治的胃癌リンパ節廓清手術時における自律神経切断の消化吸収能におよぼす影響に関する臨床的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effecs on digestion and absorption produced by a radical operation of stomach cancer with complete removal of the regional lymphnodes, studied by means of the patients subjected to the same operation, whose digestion and absorption was tested by Cr O ratio method. At the same time, for comparison, the same test was performed on patients subjected to simple gastrectomy and healthy men, with the following conclusion. 1. In patients after simple gastrectomy, absorption of fat and protein goes not so well as in healthy persons. 2. In patients after airadical operation of stomach cancer with complete removal of the regional lymphnodes, absorption of fat and protein is well performed as in patients after simple gastrectomy. 3. In patients after redical operation of stomach cancer and in those after simple gastrectomy, absorption of carbohydrate is carried on with almost the same rate, i. e. more than 99%, as in healthy persons. en-copyright= kn-copyright= en-aut-name=YoshimuraHaruo en-aut-sei=Yoshimura en-aut-mei=Haruo kn-aut-name=吉村晴夫 kn-aut-sei=吉村 kn-aut-mei=晴夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1（陣内）外科教室 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=3-4 article-no= start-page=371 end-page=377 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Metabolic changes of hepatic and renal guanidino compounds by inhalation of halothane in rats kn-title=ハロセンによる肝腎グアニジノ化合物代謝の変動に関する実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A metabolic relationship between the liver and kidney exists in combination with the urea cycle. In patients with acute renal or hepatic insufficiency, certain variations among several guanidino compounds are relevant to the condition of the liver or kidney. An experimental model of rats exposed to inhaled halothane was evaluated for metabolic changes in the liver and kidney by measuring guanidino compound levels using high performance liquid chromatography. As a result, no significant difference in serum ornithine carbamoyltransferase (OCT) activities were observed among all groups. In the liver, concentrations of creatine (CR) and guanidinobutyric acid (GBA) were significantly elevated immediately after inhalation of halothane without any accompanying changes in guanidinoacetic acid (GAA) and argnine (ARG). In the kidney, ARG and GAA were significantly decreased without any accompanying changes in CR and GBA. No significant difference in serum GC levels was observed among the groups. The inhalation of halothane appers to change the concentrations of GAA, ARG and CR in the liver and kidney. It also appers that inhalation of halothane caused GBA to appear in the blood, indicating a possible association of halothane with hepatotoxicity. en-copyright= kn-copyright= en-aut-name=KitauraMichio en-aut-sei=Kitaura en-aut-mei=Michio kn-aut-name=北浦道夫 kn-aut-sei=北浦 kn-aut-mei=道夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部麻酔・蘇生学教室 en-keyword=ハロセン kn-keyword=ハロセン en-keyword=尿素回路 kn-keyword=尿素回路 en-keyword=アルギニン kn-keyword=アルギニン en-keyword=肝腎代謝 kn-keyword=肝腎代謝 END start-ver=1.4 cd-journal=joma no-vol=122 cd-vols= no-issue=2 article-no= start-page=107 end-page=112 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Analysis of fecal DNA methylation to detect gastrointestinal neoplasia kn-title=便中メチル化DNA検出による消化器がんスクリーニング：消化器がんを非侵襲的にスクリーニングすることは可能か？ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NagasakaTakeshi en-aut-sei=Nagasaka en-aut-mei=Takeshi kn-aut-name=永坂岳司 kn-aut-sei=永坂 kn-aut-mei=岳司 aut-affil-num=1 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name=田中紀章 kn-aut-sei=田中 kn-aut-mei=紀章 aut-affil-num=2 ORCID= en-aut-name=SunDong-Sheng en-aut-sei=Sun en-aut-mei=Dong-Sheng kn-aut-name=孫冬生 kn-aut-sei=孫 kn-aut-mei=冬生 aut-affil-num=3 ORCID= en-aut-name=NaomotoYoshio en-aut-sei=Naomoto en-aut-mei=Yoshio kn-aut-name=猶本良夫 kn-aut-sei=猶本 kn-aut-mei=良夫 aut-affil-num=4 ORCID= en-aut-name=MastubaraNagahide en-aut-sei=Mastubara en-aut-mei=Nagahide kn-aut-name=松原長秀 kn-aut-sei=松原 kn-aut-mei=長秀 aut-affil-num=5 ORCID= en-aut-name=YagiTakahito en-aut-sei=Yagi en-aut-mei=Takahito kn-aut-name=八木孝仁 kn-aut-sei=八木 kn-aut-mei=孝仁 aut-affil-num=6 ORCID= en-aut-name=FujiwaraToshiyoshi en-aut-sei=Fujiwara en-aut-mei=Toshiyoshi kn-aut-name=藤原俊義 kn-aut-sei=藤原 kn-aut-mei=俊義 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬総合研究科　消化器・腫瘍外科学 affil-num=2 en-affil= kn-affil=鳥取市立病院 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬総合研究科　消化器・腫瘍外科学 affil-num=4 en-affil= kn-affil=川崎医科大学附属病院　外科 affil-num=5 en-affil= kn-affil=兵庫医科大学　外科 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬総合研究科　消化器・腫瘍外科学 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬総合研究科　消化器・腫瘍外科学 en-keyword=methylation kn-keyword=methylation en-keyword=stool kn-keyword=stool en-keyword=colorectal cancer kn-keyword=colorectal cancer en-keyword=gastric cancer kn-keyword=gastric cancer en-keyword=screening kn-keyword=screening END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=3 article-no= start-page=203 end-page=208 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Relationship between Estimated Glomerular Filtration Rate (eGFR) and Metabolic Syndrome in Japanese en-subtitle= kn-subtitle= en-abstract= kn-abstract=We investigated the link between renal function as evaluated by estimated glomerular filtration rate (eGFR) and metabolic syndrome in Japanese. A total of 11,711 Japanese subjects, aged 20-79 years, were recruited in a cross-sectional clinical investigation. From this group, we further investigated the data on 1,576 subjects. eGFR was calculated using serum creatinine (Cr), age and sex. The diagnosis of metabolic syndrome was based on the Japanese criteria. In the first analysis, 288 men (7.8%) and 498 women (6.2%) were diagnosed with reduced eGFR (<60ml/min). eGFR was not correlated with anthropometric, body composition parameters in either sex. In the second analysis, in subjects without medications, 132 men (20.8%) and 15 women (1.6%) were diagnosed with metabolic syndrome. eGFR was lower in men with abdominal obesity and in women with hypertension was than in those without. Among Japanese not taking medications, lower eGFR may be a characteristic of men with abdominal obesity and of women with hypertension. en-copyright= kn-copyright= en-aut-name=MiyatakeNobuyuki en-aut-sei=Miyatake en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShikataKenichi en-aut-sei=Shikata en-aut-mei=Kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NumataTakeyuki en-aut-sei=Numata en-aut-mei=Takeyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Hygiene, Faculty of Medicine, Kagawa University affil-num=2 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Okayama Southern Institute of Health, Okayama Health Foundation en-keyword=metabolic syndrome kn-keyword=metabolic syndrome en-keyword=estimated glomerular filtration rate (eGFR) kn-keyword=estimated glomerular filtration rate (eGFR) en-keyword=abdominal circumference kn-keyword=abdominal circumference END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=10 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20049 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structure and magnetic properties of the single-molecule magnet [Mn11CrO12(O2CCH3)(16)(H2O)(4)]center dot 2CH(3)COOH center dot 4H(2)O: magnetization manipulation and dipolar-biased tunneling in a Mn11Cr/Mn-12 mixed crystal en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The structural and magnetic properties of the single-molecule magnet [Mn11CrO12(O2CCH3)(16)(H2O)(4)].2CH(3)COOH.4H(2)O (Mn11Cr) are studied through the analysis of a Mn11Cr/Mn-12 (approximate to1:1) mixed crystal, where Mn-12 is [Mn12O12(O2CCH3)(16)(H2O)(4)].2CH(3)COOH.4H(2)O. X-ray absorption spectra reveal that the Cr ion in Mn11Cr is in the +3 valence state and occupies a specific Mn3+ site in the Mn-12 skeleton. High-frequency electron paramagnetic resonance (EPR) spectra are well explained by assuming that Mn11Cr is in a ground spin-state of S=19/2 with nearly the same EPR parameter set as for Mn-12. The lower spin quantum number results in lower barrier height (56.8 K) compared to Mn-12. The magnetization curves indicate a coercive field of 0.95 T for Mn11Cr at 1.8 K, nearly half that for Mn-12. Quantum tunneling of magnetization (QTM) in Mn11Cr is observed below the blocking temperature T-B, with the same field interval as for Mn-12. The magnetization of Mn11Cr and Mn-12 in the mixed crystal can be independently manipulated by utilizing the difference between their coercive fields. The resonance fields of QTM in Mn11Cr are significantly affected by the magnetization direction of Mn-12, suggesting the effect of dipolar-biased tunneling.

en-copyright= kn-copyright= en-aut-name=HachisukaHidekazu en-aut-sei=Hachisuka en-aut-mei=Hidekazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AwagaKunio en-aut-sei=Awaga en-aut-mei=Kunio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YokoyamaToshihiko en-aut-sei=Yokoyama en-aut-mei=Toshihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KuboTakeji en-aut-sei=Kubo en-aut-mei=Takeji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=GotoTakao en-aut-sei=Goto en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NojiriHiroyuki en-aut-sei=Nojiri en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Nagoya University affil-num=2 en-affil= kn-affil=Nagoya University affil-num=3 en-affil= kn-affil=Institute for Molecular Sciences affil-num=4 en-affil= kn-affil=Nara University of Education affil-num=5 en-affil= kn-affil=Graduate School of Human and Environmental Studies affil-num=6 en-affil= kn-affil=Okayama University en-keyword=high-spin molecules kn-keyword=high-spin molecules en-keyword=jahn-teller isomerism kn-keyword=jahn-teller isomerism en-keyword=ground-state kn-keyword=ground-state en-keyword=mn-12 kn-keyword=mn-12 en-keyword=complexes kn-keyword=complexes en-keyword=relaxation kn-keyword=relaxation en-keyword=susceptibility kn-keyword=susceptibility en-keyword=mn12012(02cr)(16)(H20)(4) kn-keyword=mn12012(02cr)(16)(H20)(4) en-keyword=mn12012(02CR)16(h20)4 kn-keyword=mn12012(02CR)16(h20)4 en-keyword=cluster kn-keyword=cluster en-keyword=s=9 kn-keyword=s=9 END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=2 article-no= start-page=388 end-page=393 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20051013 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=On-line preconcentration using dual mini-columns for the speciation of chromium(III) and chromium(VI) and its application to water samples as studied by inductively coupled plasma-atomic emission spectrometry en-subtitle= kn-subtitle= en-abstract= kn-abstract=

On-line preconcentration system for the selective, sensitive and simultaneous determination of chromium species was investigated. Dual minicolumns containing chelating resin were utilized for the speciation and preconcentration of Cr(III) and Cr(VI) in water samples. In this system, Cr(III) was collected on first column packed with iminodiacetate resin. Cr(VI) in the effluent from the first column was reduced to Cr(III), which was collected on the second column packed with iminodiacetate resin. Hydroxyammonium chloride was examined as a potential reducing agent for Cr(VI) to Cr(III). The effects of pH, sample flow rate, column length, and interfering ions on the recoveries of Cr(III) were carefully studied. Five millilitres of a sample solution was introduced into the system. The collected species were then sequentially washed by 1 M ammonium acetate, eluted by 2 M nitric acid and measured by ICP-AES. The detection limit for Cr(III) and Cr(VI) was 0.08 and 0.15 mu g l-1, respectively. The total analysis time was about 9.4 min. The developed method was successfully applied to the speciation of chromium in river, tap water and wastewater samples with satisfied results.

en-copyright= kn-copyright= en-aut-name=SumidaTakashi en-aut-sei=Sumida en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IkenoueTamami en-aut-sei=Ikenoue en-aut-mei=Tamami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HamadaKazuhide en-aut-sei=Hamada en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=5 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Industrial Technology Center of Kochi Prefecture affil-num=2 en-affil= kn-affil=Industrial Technology Center of Kochi Prefecture affil-num=3 en-affil= kn-affil=Industrial Technology Center of Kochi Prefecture affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=6 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=speciation kn-keyword=speciation en-keyword=chromium kn-keyword=chromium en-keyword=on-line preconcentration kn-keyword=on-line preconcentration en-keyword=ICP-AES kn-keyword=ICP-AES en-keyword=chelating resin kn-keyword=chelating resin END start-ver=1.4 cd-journal=joma no-vol=22 cd-vols= no-issue=1 article-no= start-page=161 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060224 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Speciation of chromium in seawater by ICP-AES with dual mini-columns containing chelating resin en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A method for the preconcentration and speciation of chromium in seawater was developed. On-line preconcentration and determination were carried out by using inductively coupled plasma atomic emission spectrometry (ICP-AES) with dual mini-columns containing a chelating resin. In this system, Cr(III) was collected on the first column. The effluent containing residual chromium from the first column was collected on the second column after passing through a reduction-switching unit, in which the reducing agent was introduced, or not, for the reduction of Cr(VI) to Cr(Ill). Cr(VI) was determined as the difference between the concentration of pre-reduced Cr(VI) and Cr(III) in the effluent from the first column. The detection limits for Cr(III) and Cr(VI) were 0.04 and 0.09 mu g l(-1), respectively.

en-copyright= kn-copyright= en-aut-name=SumidaTakashi en-aut-sei=Sumida en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kochi Prefectual Industrial Technology Center affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=ONLINE PRECONCENTRATION kn-keyword=ONLINE PRECONCENTRATION en-keyword=SPECTROMETRY kn-keyword=SPECTROMETRY END start-ver=1.4 cd-journal=joma no-vol=55 cd-vols= no-issue=1 article-no= start-page=41 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=200102 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Experimental study of fast and ultrafast T2-weighted imaging sequences using AMI-25 superparamagnetic iron oxide (SPIO). en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The objective of this study was to evaluate fast and ultrafast T2-weighted images (T2WI), including echo planar imaging (EPI), using an AMI-25 agar phantom. Image quality for conventional spin echo (CSE) and turbo spin echo (TSE) was almost equivalent. In high-resolution TSE, image quality was highest due to the use of a 512 x 256 matrix. Half-Fourier single-shot turbo SE (HASTE) was associated with blurring of images, and turbo-gradient SE (TGSE) showed a deterioration of image quality. EPI also suffered from poor image quality because this method is very sensitive to magnetic field inhomogeneity. CSE showed good signal-to-noise ratio (S/N) and contrast ratio (CR), but also required the longest imaging times. Among the TSE sequences, TSE with a short echo train length (ETL) was superior in terms of S/N. The CR of EPI and fast low angle shot (FLASH) images were improved in proportion to the effective echo time (TE). At present, TSE is inferior to CSE in terms of S/N and CR. However, taking into consideration scanning time, TSE with a short ETL is thought to be suitable for routine examinations. Effective TE is an important factor in gradient echo (GRE) examinations.</P>

en-copyright= kn-copyright= en-aut-name=KurokawaHironori en-aut-sei=Kurokawa en-aut-mei=Hironori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TogamiIzumi en-aut-sei=Togami en-aut-mei=Izumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsunodaMasatoshi en-aut-sei=Tsunoda en-aut-mei=Masatoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HikariYoshio en-aut-sei=Hikari en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=MRI kn-keyword=MRI en-keyword= SPIO(superparamagnetic iron oxide) kn-keyword= SPIO(superparamagnetic iron oxide) en-keyword=liver phantom kn-keyword=liver phantom en-keyword= various T2WI(T2-weighted images) kn-keyword= various T2WI(T2-weighted images) END start-ver=1.4 cd-journal=joma no-vol=40 cd-vols= no-issue=3 article-no= start-page=175 end-page=177 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=198606 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Aclarubicin in the treatment of elderly patients with acute nonlymphocytic leukemia. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Thirteen previously untreated patients aged 70 and above with acute nonlymphocytic leukemia were treated with aclarubicin (ACR) alone. Among 10 cases (3, acute myelocytic leukemia; 4, acute myelomonocytic leukemia; 2, acute monocytic leukemia; and one, acute erythroleukemia) in which an evaluation was possible, 5 cases (3, acute myelomonocytic leukemia; and 2, acute monocytic leukemia) obtained complete remission (CR). The CR rate was 83% in 6 patients with acute myelomonocytic leukemia or acute monocytic leukemia. The median CR duration and survival was 7.5 and 10 + months, respectively. Although side effects of the drug on digestive system such as nausea, vomiting and anorexia were observed in all patients, they were controllable by conventional treatments. The results suggest that ACR is effective for the clinical management of elderly patients with acute nonlymphocytic leukemia, especially those with acute myelomonocytic leukemia or acute monocytic leukemia.

en-copyright= kn-copyright= en-aut-name=TakahishiIsao en-aut-sei=Takahishi en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YorimitsuSeiichi en-aut-sei=Yorimitsu en-aut-mei=Seiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HaraMasamichi en-aut-sei=Hara en-aut-mei=Masamichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=InagakiToshihiro en-aut-sei=Inagaki en-aut-mei=Toshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakadaHiroyuki en-aut-sei=Nakada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SekitoNoriko en-aut-sei=Sekito en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HayashiNaoki en-aut-sei=Hayashi en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=Nonakakenichi en-aut-sei=Nonaka en-aut-mei=kenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OhmotoEijiro en-aut-sei=Ohmoto en-aut-mei=Eijiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=UchidaKouzaburo en-aut-sei=Uchida en-aut-mei=Kouzaburo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TakizawaMichihiro en-aut-sei=Takizawa en-aut-mei=Michihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=SanadaHiroshi en-aut-sei=Sanada en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=AdachiTomiro en-aut-sei=Adachi en-aut-mei=Tomiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=TsubotaTeruhiko en-aut-sei=Tsubota en-aut-mei=Teruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=KitajimaKoichi en-aut-sei=Kitajima en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University affil-num=11 en-affil= kn-affil=Okayama University affil-num=12 en-affil= kn-affil=Okayama University affil-num=13 en-affil= kn-affil=Okayama University Hospital affil-num=14 en-affil= kn-affil=Okayama Shimin Hospital affil-num=15 en-affil= kn-affil=Okayama Shimin Hospital affil-num=16 en-affil= kn-affil=Okyama University School of Allied Medical Science en-keyword=acute leukemia in elderly patients kn-keyword=acute leukemia in elderly patients en-keyword=chemotherapy of acute leukemia kn-keyword=chemotherapy of acute leukemia en-keyword=aclarubicin kn-keyword=aclarubicin END start-ver=1.4 cd-journal=joma no-vol=53 cd-vols= no-issue=3 article-no= start-page=133 end-page=140 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Urinary excretion of type I collagen cross-linked N-telopeptides, bone mass and related lifestyle in middle-aged women. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The relationship between past and present lifestyle and urinary excretion of type I collagen cross-linked N-telopeptides (NTx) was studied in 61 Japanese females aged 34-59, with a view toward using NTx excretion rates as a predictor of future osteoporosis. Bone mineral density (BMD) of the lumbar spine, the speed of sound (SOS) and broadband ultrasound attenuation (BUA) of the os calcis, urinary NTx, serum osteocalcin (BGP) and bone-specific alkaline phosphatase (BAP) were measured. Stiffness index (stiffness) was calculated from SOS and BUA. The subjects were asked whether they took regular exercise in their childhood and teen years (in elementary, junior-high, senior-high school and college), the past (20-40 years of age) and present adulthood. Regular calcium intake, smoking habits, alcohol and other beverage consumption and milk consumption were also covered in the questionnaire. The mean NTx values of premenopausal and postmenopausal group were 22.2 and 56.0 nM bone collagen equivalents (BCE)/mM urinary creatinine (Cr), respectively. The group which did not exercise regularly between the ages of 20 and 40 had a higher mean NTx value (40.9 nMBCE/mMCr) than the group which did exercise regularly (22.7 nMBCE/mMCr). In multiple regression analyses, age, stiffness and exercise in past adulthood could explain 43.5% of the NTx variance. For prevention of bone metabolic increases around menopause, habitual exercise in early adulthood seems to be effective.

en-copyright= kn-copyright= en-aut-name=MasatomiChie en-aut-sei=Masatomi en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ImaiKaori en-aut-sei=Imai en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WangDa-Hong en-aut-sei=Wang en-aut-mei=Da-Hong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IkedaSatoru en-aut-sei=Ikeda en-aut-mei=Satoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakataShinji en-aut-sei=Takata en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KiraShohei en-aut-sei=Kira en-aut-mei=Shohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Ibara City Hospital affil-num=6 en-affil= kn-affil=Okayama Saiseikai General Hospital affil-num=7 en-affil= kn-affil=Okayama University en-keyword=N-telopeptides kn-keyword=N-telopeptides en-keyword=bone turnover kn-keyword=bone turnover en-keyword=osteoporosis kn-keyword=osteoporosis en-keyword=menopause kn-keyword=menopause en-keyword=lifestyle kn-keyword=lifestyle END start-ver=1.4 cd-journal=joma no-vol=31 cd-vols= no-issue=1 article-no= start-page=71 end-page=80 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197702 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=COP, a new alloy for surgical implants en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Today Vitallium is used for surgical implants. It is a casting alloy which, with advances in casting technology, is also used commercially for making instruments of fairly complex shape. Because of its expense, however, it is not widely used in Japan. Instead, a series of 18-8 Mo alloys are used in Japan even though of insufficient strength. Used over a long period of time in the body, especially for the purpose of preserving structual functions as part of the human skeleton, it often corrodes, resulting in either abnormalities in tissue cells or, because of its insufficient strength, danger of bending and breaking with aging. In spite of a marked advance in fracture treatment, we have hardly any suitable materials for making instruments appropriate to the internal fixation of fractures in Japan. We, therefore, conducted various experiments to develop an alloy with sufficient corrosive resistance and strength that could be formed into a complex shape to take the place of Vitallium alloy, finally succeeding in developing an alloy we call "COP". The characteristic properties of COP may be summarized as follows: 1. The main components are 20% Cr, 20% Ni, 20% Co and 4% Mo aside from 0.2% P. 2. As it contains "P", it shows a marked age-hardening. In its molten state its machinability is excellent, and later it can readily be hardened by heat-treatment. 3. It has not only a marked yield point and tensile strength but also has toughness in elongation and reduction of area, showing a strength which surpasses Vitallium. 4. Its corrosive resistance is great. 5. Its cost is far cheaper than Vitallium.

en-copyright= kn-copyright= en-aut-name=SunamiYoshifumi en-aut-sei=Sunami en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IshikawaEijiroo en-aut-sei=Ishikawa en-aut-mei=Eijiroo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Tokushu Seiko Co. Ltd. END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=3 article-no= start-page=145 end-page=150 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199606 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Regulation of Interleukin-2 Receptor y Chain mRNA Expression in Human Monocytic Cell Line THP-1 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P < 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.

en-copyright= kn-copyright= en-aut-name=YanaiHiroyuki en-aut-sei=Yanai en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NinomiyaYoshifumi en-aut-sei=Ninomiya en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=IL-2R ??chain kn-keyword=IL-2R ??chain en-keyword=phorbol ester kn-keyword=phorbol ester en-keyword=monocyte kn-keyword=monocyte en-keyword=differentiation kn-keyword=differentiation en-keyword=protein kinase kn-keyword=protein kinase END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=3 article-no= start-page=153 end-page=159 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Increased urinary excretion of non-albumin antigen detected with YO-2, a novel monoclonal antibody, in diabetic patients. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were not reactive with an anti-human albumin antibody. The urine levels of YO-2-reactive antigen in the normal control were 0.97 +/- 0.37 U/g-Cr (units per gram of urine creatinine) (mean +/- SD). Those of the normo-, micro-, and macroalbuminuric diabetic patients, respectively, were 1.38 +/- 1.36, 2.87 +/- 2.07, and 3.92 +/- 3.33 U/g-Cr. They were significantly higher in the micro- and macroalbuminuric patients. The urine levels of YO-2-reactive antigen had no significant correlation with the urine albumin levels and hemoglobin A1c. We concluded that; a) monoclonal antibody YO-2 recognized a non-albumin urine antigen increasingly excreted in diabetic patients with nephropathy, b) recent glycemic control of diabetes would not significantly affect the urinary excretion rate of YO-2-reactive antigen, and c) the excretion rate and probably the mechanism of YO-2-reactive protein differed from those of albumin. The urine levels of YO-2-reactive antigen could be a clinical marker of diabetic nephropathy.

en-copyright= kn-copyright= en-aut-name=YoneiTaiji en-aut-sei=Yonei en-aut-mei=Taiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WataraiShinobu en-aut-sei=Watarai en-aut-mei=Shinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkadaYoshio en-aut-sei=Okada en-aut-mei=Yoshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YasudaTatsuji en-aut-sei=Yasuda en-aut-mei=Tatsuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=diabetes kn-keyword=diabetes en-keyword=nephropathy kn-keyword=nephropathy en-keyword=monoclonal antibody kn-keyword=monoclonal antibody en-keyword=microalbuminuria kn-keyword=microalbuminuria END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ユレイライトのMn-Cr年代：初期太陽系におけるCr同位体構造の解釈 kn-title=Mn-Cr age of monomict ureilites: implications for Cr isotopic structure of the early inner solar system en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamakawaAkane en-aut-sei=Yamakawa en-aut-mei=Akane kn-aut-name=山川茜 kn-aut-sei=山川 kn-aut-mei=茜 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue= article-no= start-page=4 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Determination of trace metals in environmental water samples by inductively coupled plasma - mass spectrometry (ICP-MS) kn-title=誘導結合プラズマ―質量分析法による環境水中の微量金属の定量 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Trace metals in water samples, such as tap water, river water, and sea water, were determined by inductively coupled plasma-mass spectrometry (ICP-MS). ICP-MS has sufficient sensitivity to detect even below the concentrations of ppt level, and therefore the samples were not pretreated with a concentration column. Practical samples were diluted by lo-fold with ultrapure water and measured directly by ICP-MS. The measuring time of one sample was 2.5 min, and the data for 30 elements were obtained simultaneously. The concentrations of heavy metals, such as Cr, Mn, Co, Ni, Cu, Zn, As, Cd, Sb, and Pb, in the water samples were in the ranges of 0.05~82ng/ml. Zasu river and Sibukawa (sea water) contained various kinds of metals, and the concentration ranges were spread in a wide range; for example, Mg was 25700 ppb and In was 2 ppt. ICP-MS is found to be a useful and a powerful instrument for trace amounts of elements, and can be applied satisfactorily to the environmental water analysis. en-copyright= kn-copyright= en-aut-name=LeeKyue-Hyung en-aut-sei=Lee en-aut-mei=Kyue-Hyung kn-aut-name=李啓熒 kn-aut-sei=李 kn-aut-mei=啓熒 aut-affil-num=1 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=2 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name=高柳俊夫 kn-aut-sei=高柳 kn-aut-mei=俊夫 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学理学部化学科 affil-num=2 en-affil= kn-affil=岡山大学理学部化学科 affil-num=3 en-affil= kn-affil=岡山大学理学部化学科 affil-num=4 en-affil= kn-affil=岡山大学理学部化学科 END start-ver=1.4 cd-journal=joma no-vol=29 cd-vols= no-issue= article-no= start-page=11 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=200710 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Material recycling of blast furnace slag by phase separation of glass kn-title=ガラスの分相現象を利用した高炉水砕スラグの再資源化技術の開発 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel recycling process of blast furnace slag was developed in order to obtain colorless silica－rich solids by using phase separation of borosilicate glass. B(2)O(3) was added to blast furnace slag to promote the phase separation. The slag glasses were heat-treated above glass transition temperatures. The slag glass prepared from blast furnace slag gelled after the heat treatment and the subsequent three types of acid treatment. The ratios of SiO(2) component in the gels were 40 - 60mass%. On the other hand, phase separation was observed on the surface of the slag glasses prepared from pre-treated slag by 2.5N HCI after the heat treatment. After soaking in acid, they did not gel and changed to insoluble colorless solids. According to compositional analyses, it was found that the insoluble colorless solids contained 70 - 90mass% SiO(2). No colored ions such as Cr, Mn, and Fe were confirmed in the remaining insolubles by optical absorption measurement. Therefore, the colorless silica-rich solids were successfully obtained in the present process. The end products obtained in the present process are expected as material of glass. en-copyright= kn-copyright= en-aut-name=SakidaShinichi en-aut-sei=Sakida en-aut-mei=Shinichi kn-aut-name=崎田真一 kn-aut-sei=崎田 kn-aut-mei=真一 aut-affil-num=1 ORCID= en-aut-name=MikamiSyuuhei en-aut-sei=Mikami en-aut-mei=Syuuhei kn-aut-name=三上修平 kn-aut-sei=三上 kn-aut-mei=修平 aut-affil-num=2 ORCID= en-aut-name=NanbaTokuro en-aut-sei=Nanba en-aut-mei=Tokuro kn-aut-name=難波徳郎 kn-aut-sei=難波 kn-aut-mei=徳郎 aut-affil-num=3 ORCID= en-aut-name=MiuraToshinari en-aut-sei=Miura en-aut-mei=Toshinari kn-aut-name=三浦嘉也 kn-aut-sei=三浦 kn-aut-mei=嘉也 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学保健環境センター環境安全部門 affil-num=2 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=3 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 affil-num=4 en-affil= kn-affil=岡山大学環境理工学部環境物質工学科 en-keyword=Blast furnace slag kn-keyword=Blast furnace slag en-keyword=Recycling kn-keyword=Recycling en-keyword=Colorless silica-rich solids kn-keyword=Colorless silica-rich solids en-keyword=Phase separation kn-keyword=Phase separation en-keyword=Glass kn-keyword=Glass END start-ver=1.4 cd-journal=joma no-vol=122 cd-vols= no-issue=1 article-no= start-page=17 end-page=25 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Dual tyrosine kinase inhibitor for focal adhesion kinase and insulin-like growth factor-I receptor exhibits an anticancer effect in esophageal adenocarcinoma in vitro and in vivo kn-title=Focal adhesion kinase (FAK) と Insulin-like growth factor-I receptor (IGF-IR) に対するデュアルチロシンキナーゼ阻害剤の食道腺癌における抗腫瘍効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WatanabeNobuyuki en-aut-sei=Watanabe en-aut-mei=Nobuyuki kn-aut-name=渡辺信之 kn-aut-sei=渡辺 kn-aut-mei=信之 aut-affil-num=1 ORCID= en-aut-name=TakaokaMunenori en-aut-sei=Takaoka en-aut-mei=Munenori kn-aut-name=髙岡宗徳 kn-aut-sei=髙岡 kn-aut-mei=宗徳 aut-affil-num=2 ORCID= en-aut-name=SakuramaKazufumi en-aut-sei=Sakurama en-aut-mei=Kazufumi kn-aut-name=櫻間一史 kn-aut-sei=櫻間 kn-aut-mei=一史 aut-affil-num=3 ORCID= en-aut-name=TomonoYasuko en-aut-sei=Tomono en-aut-mei=Yasuko kn-aut-name=友野靖子 kn-aut-sei=友野 kn-aut-mei=靖子 aut-affil-num=4 ORCID= en-aut-name=HatakeyamaShinji en-aut-sei=Hatakeyama en-aut-mei=Shinji kn-aut-name=畠山慎二 kn-aut-sei=畠山 kn-aut-mei=慎二 aut-affil-num=5 ORCID= en-aut-name=OhmoriOsamu en-aut-sei=Ohmori en-aut-mei=Osamu kn-aut-name=大森修 kn-aut-sei=大森 kn-aut-mei=修 aut-affil-num=6 ORCID= en-aut-name=MotokiTakayuki en-aut-sei=Motoki en-aut-mei=Takayuki kn-aut-name=元木崇之 kn-aut-sei=元木 kn-aut-mei=崇之 aut-affil-num=7 ORCID= en-aut-name=ShirakawaYasuhiro en-aut-sei=Shirakawa en-aut-mei=Yasuhiro kn-aut-name=白川靖博 kn-aut-sei=白川 kn-aut-mei=靖博 aut-affil-num=8 ORCID= en-aut-name=YamatsujiTomoki en-aut-sei=Yamatsuji en-aut-mei=Tomoki kn-aut-name=山辻知樹 kn-aut-sei=山辻 kn-aut-mei=知樹 aut-affil-num=9 ORCID= en-aut-name=HaisaMinoru en-aut-sei=Haisa en-aut-mei=Minoru kn-aut-name=羽井佐実 kn-aut-sei=羽井佐 kn-aut-mei=実 aut-affil-num=10 ORCID= en-aut-name=MatsuokaJunji en-aut-sei=Matsuoka en-aut-mei=Junji kn-aut-name=松岡順治 kn-aut-sei=松岡 kn-aut-mei=順治 aut-affil-num=11 ORCID= en-aut-name=BeerDavid G. en-aut-sei=Beer en-aut-mei=David G. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NagatsukaHitoshi en-aut-sei=Nagatsuka en-aut-mei=Hitoshi kn-aut-name=長塚仁 kn-aut-sei=長塚 kn-aut-mei=仁 aut-affil-num=13 ORCID= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name=田中紀章 kn-aut-sei=田中 kn-aut-mei=紀章 aut-affil-num=14 ORCID= en-aut-name=NaomotoYoshio en-aut-sei=Naomoto en-aut-mei=Yoshio kn-aut-name=猶本良夫 kn-aut-sei=猶本 kn-aut-mei=良夫 aut-affil-num=15 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=4 en-affil= kn-affil=重井医学研究所 affil-num=5 en-affil= kn-affil=ノバルティスファーマ　筑波研究所 affil-num=6 en-affil= kn-affil=ノバルティスファーマ　筑波研究所 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=8 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=9 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=10 en-affil= kn-affil=岡山市立市民病院　外科 affil-num=11 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=12 en-affil= kn-affil=ミシガン大学　胸部外科 affil-num=13 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　口腔病理学 affil-num=14 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 affil-num=15 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　消化器・腫瘍外科学 en-keyword=focal adhesion kinase (FAK) kn-keyword=focal adhesion kinase (FAK) en-keyword=バレット食道癌 kn-keyword=バレット食道癌 en-keyword=FAK阻害剤 kn-keyword=FAK阻害剤 END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=3 article-no= start-page=103 end-page=108 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890305 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Spectrophotometric determination of copper with iodide ion and Crystal Violet kn-title=ヨウ化物イオンとクリスタルバイオレットを用いる銅の吸光光度定量 en-subtitle= kn-subtitle= en-abstract=A method for spectrophotometric determination of micro amounts of copper with iodide ion and Crystal Violet (CV) was developed. The method is based on the formation of triiodide ion by the oxidative reaction of iodide ion with copper(II) in dilute sulfuric acid solution and the extraction of triiodide ion into toluene as an ion association complex with CV. The main extracted species is expected to be an ion association complex, CV(+)I(-)(3) copper(II) acts as a catalyst. The recommended procedure is as follows. Take a sample solution containing copper(II) up to 1.6 μg into a 25-ml stoppered test tube. Add 1 ml of 0.46 M sodium iodide solution, 1 ml of 0.5 M sulfuric acid solution and 1 ml of 3.2×10(-4)M CV solution, and dilute the solution to 10 ml with distilled water. Shake the mixture with 5 ml of toluene for 8 min. After the phase separation, measure the absorbance of the organic phase at 607 nm against the reagent blank. The calibration graph shows linearity up to 2.5×10(-6) M of copper(II) in the aqueous phase, and the apparent molar absorptivity is 1.7×10(5)1 mol(-1) cm(-1). Small amounts of oxidizing and reducing agents such as Fe(III), Cr(III) and Co(II) interfered with the determination. The present method is very simple and has a high sensitivity. kn-abstract=硫酸酸性溶液中でCu(2+)に対して過剰のI(-)が存在すると,I(-)は酸化されて三ヨウ化物イオン(I(-)(3))となり,Cu(2+)はジヨード銅(I)酸イオン(CuI(-)(2))となる.生成したI(-)(3),CuI(-)(2)はクリスタルバイオレット(CV(+))の存在下でイオン会合体となり,トルエンに抽出される.このトルエン相の607nmにおける吸光度を測定すれば,銅を定量することができる.検量線より算出した見掛けのモル吸光係数は1.7×10(5)1 mol(-1)cm(-1)であった.Fc(III),Cr(III),Co(II)などの酸化性及び還元性物質の共存は定量を妨害するが,その他多くのイオンは多量共存しても妨害とはならない.更に抽出化学種について検討した結果,主な抽出種はI(-)(3)とCV(+)の1:1のイオン会合体であり,大部分の銅イオンは水相に残っていることを確認した.これらのことから,本抽出系では銅(II)イオンは触媒的作用をしているものと結論された. en-copyright= kn-copyright= en-aut-name=YamamotoKoichi en-aut-sei=Yamamoto en-aut-mei=Koichi kn-aut-name=山本幸市 kn-aut-sei=山本 kn-aut-mei=幸市 aut-affil-num=1 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=米子工業高等専門学校工業化学科 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=spectrophotometric dtermination of copper with iodide ion and Crystal Violet kn-keyword=spectrophotometric dtermination of copper with iodide ion and Crystal Violet en-keyword=extraction of ion association complex into toluene kn-keyword=extraction of ion association complex into toluene END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=3 article-no= start-page=957 end-page=964 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=1958 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Anticonvulsive Effects of Diamox. Part Ⅴ.The Effects of Diamox on the Keto-enol Granula in the Brain. kn-title=Diamoxの抗痙攣効果に関する研究 第5編 Diamoxの脳髄ケトエノール顆粒に及ぼす影響 en-subtitle= kn-subtitle= en-abstract=The effects of Diamox on Cr K.E.G. and Hg K.E.G. of the nerve cells in cerebral cortex and Nissl gray of cats brain by Hamazaki K.E.G. method. Concerning about the (experimental) results of each 6 cases, on Cr K.E.G., the granula increased as approximately twice as much in both of the nerve cells and Nissle gray at 9 hours and then gradually decreased at 24 hours, but they were still suspected to be much more than the normal, while Hg K.E.G. showed no remarkable changes after the injection. This increasing of granula is considered to be caused by acceleration of metabolism in the brain. kn-abstract= en-copyright= kn-copyright= en-aut-name=KawaharaYoshimasa en-aut-sei=Kawahara en-aut-mei=Yoshimasa kn-aut-name=河原佳正 kn-aut-sei=河原 kn-aut-mei=佳正 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=72 cd-vols= no-issue=3 article-no= start-page=821 end-page=830 dt-received= dt-revised= dt-accepted= dt-pub-year=1960 dt-pub=19600228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Sulfur Metabolism in Infants Part I. Some Suggestion for the Quautative Analysis of Total Sulfur in Milk, Faces and Urine and the Total Sulfur Contents in Various Milk kn-title=乳児の硫黄代謝に関する研究 第1編 乳汁，屎及び尿中の総硫黄定量法の考案並びに各種乳汁中の総硫黄量 en-subtitle= kn-subtitle= en-abstract= kn-abstract=There are numerous articles concerning the estination of total sulfur in organic substances and yet the results obtained hitherto are not by any means quite satisfactory. The gratest difficulty encountered in the estination of sulfur seems to lie in the insufficient ashing of the substance. Therefore, the author studied various ashing metheds and finally modified Bethge's wet ashing in the following manner: 1, Ammoniated vanadium (NH(4)VO(3)) is added as an oxidizing catalyst. 2. As an indicator of the complete oxidation potassium chromate (K(2)Cr(2)O(7)) is used. 3. The ratio of acid mixture is set in the proportion of three parts perohloric acid to two parts nitric acid. 4. The reduction mixture is placed into an ampule filled with pitrogan immediately after it is prepared and is sealed tightly, and the quantity of the mixture to be added is increased. By the modified method mentioned above it has been possible to raise the recovery rate of sulfur added as much as 95.4 per Gent, proving that this method is sufficiently useful for the estimation of the total sulfur in milk, urine and faces. In the estimations of total sulfur of various milk such as breast milk, milk on the market and 18% solution of regulated powered milk the average contents of total sulfur have been found to be 11.7 mg and 31.8 mg per dl respectively. en-copyright= kn-copyright= en-aut-name=HamadaGibun en-aut-sei=Hamada en-aut-mei=Gibun kn-aut-name=濱田義文 kn-aut-sei=濱田 kn-aut-mei=義文 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科教室 END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1-2 article-no= start-page=39 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=19760228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effects of heavy metals on developing sea urchin eggs. kn-title=ウニ卵の発生に及ぼす重金属の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of heavy metals, such as Hg, Pb, Cu, Cd, As, Sn, Cr, Mn, Ni and Zn on the two developing sea urchin eggs. Temnopleurus and Hemicentrotus were investigated. The results obtained were as follows. 1. The development of fertilized eggs was inhibited when embryos were exposed to 10(-6) M of HgCl(2) and CuCl(2) and to 10(-4) M of CrCl(2) and NiCl(2) in Herbst's artificial seawater from fertilization until the formation of the blastula larva. 2. Chlorate and acetate of heavy metals were dissolved in filtered seawater. The morphological differences of embryo were not observed until the formation of the pluteus larva in the two solutions. Inhibitory effects were observed when embryos were exposed to seawater at concentrations of 10(-7) M of Hg and Cu, to 10(-6) M of Cd, and to 10(-5) M of Pb, Ni and Zn. At a concentration of 10(-4) M of Cd there were no visible effects until the appearance of swimming blastula larva, but larva development was strongly inhibited at time of bonification from pluteus larva, and there was no further later development. In 10(-6) M Hg, Cr, and Ni, the larvae were structural abnonal and shaped like Apollo. In 10(-7) M of Hg exogastrula embryos were produced. 3. Threshold concentration of complete inhibition on the early development of sea urchin embryos was at 10(-6) M of Hg, 10(-5) M of Cu, Cr, Ni, and Zn, 10(-3) M of Mn. In the case of Cd 10(-5) M, complete inhibition didn't occur until the pluteus larva stage. 4. Only additive effects were obtained on the developing sea urchin eggs when two heavy metals were combined in filtered seawater. A high frequency of morphological abnormalities were formed such as exogastrula and Apollo shaped pluteus especially in coupling of Hg and Cu or Cd and Zn. 5. There were no visible effects in filtered sewage from fertilization to the formation of the pluteus larva. When embryos were exposed to acetate salts of Hg, Cu, Cd, and Zn in filtered sewage, there were 10 fold inhibitory effects on development. But formation of skeleton in the pluteus larva was found in filtered sewage containing Cd. 6. Only additive effects were observed on sea urchin embryo when combined with Hg, Cu, Cd, and Zn in filterd sewage. But the abnormalities were decreased in all cases. Inhibition of bonification were reduced in mixture of Cd and filtered sewage. en-copyright= kn-copyright= en-aut-name=MurakamiTetuhide en-aut-sei=Murakami en-aut-mei=Tetuhide kn-aut-name=村上哲英 kn-aut-sei=村上 kn-aut-mei=哲英 aut-affil-num=1 ORCID= en-aut-name=HayakawaMasashi en-aut-sei=Hayakawa en-aut-mei=Masashi kn-aut-name=早川昌志 kn-aut-sei=早川 kn-aut-mei=昌志 aut-affil-num=2 ORCID= en-aut-name=FujiiToshitake en-aut-sei=Fujii en-aut-mei=Toshitake kn-aut-name=藤井和武 kn-aut-sei=藤井 kn-aut-mei=和武 aut-affil-num=3 ORCID= en-aut-name=HaraTakehito en-aut-sei=Hara en-aut-mei=Takehito kn-aut-name=原武仁 kn-aut-sei=原 kn-aut-mei=武仁 aut-affil-num=4 ORCID= en-aut-name=ItamiYoshiaki en-aut-sei=Itami en-aut-mei=Yoshiaki kn-aut-name=伊丹義明 kn-aut-sei=伊丹 kn-aut-mei=義明 aut-affil-num=5 ORCID= en-aut-name=KishidaAkira en-aut-sei=Kishida en-aut-mei=Akira kn-aut-name=岸田昭 kn-aut-sei=岸田 kn-aut-mei=昭 aut-affil-num=6 ORCID= en-aut-name=NisidaIsamu en-aut-sei=Nisida en-aut-mei=Isamu kn-aut-name=西田勇 kn-aut-sei=西田 kn-aut-mei=勇 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部第一生理学教室 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=3-4 article-no= start-page=369 end-page=377 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of tumor-BCG mixture to the cellular cytotoxicity against tumor allograft kn-title=同種腫瘍移植に於けるCellular CytotoXiCityに及ぼすBCG―腫瘍混合接種の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of tumor-BCG mixture to the cellular cytotoxicity of the regional lymph node and the spleen were observed in BALB/c mice received allogeneic tumor cells. This tumor cells, when grafted subcutaneously, were normally enhanced in 40% of BALB/c mice and 100% of A/St mice, but failed to grow when injected as a mixture with tumor and BCG. By injection of tumor-BCG mixture, the development and decline of the cytotoxicity((51)Cr release assay) to the tumor cells was accelerated, but the magnitude of the peak activity was diminished. Possible mechanisms by which the decreased activity induced of complete tumor suppression were examined. The result showed that immunized lymphocytes with non-immune macrophages exhibited a significatly increased cytotoxicity as compared with immunized lymphocytes in the abscence of macrophages. en-copyright= kn-copyright= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name=田中紀章 kn-aut-sei=田中 kn-aut-mei=紀章 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科教室 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=3-4 article-no= start-page=355 end-page=367 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immune response to tumor allograft: comparison of two in vitro assays of cell mediated cytotoxicity kn-title=同種腫瘍移植に対するcellular cytotoxicity 二つのin vitro assayの比較 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A microcytotoxicity assay (MCA) and a (51)Cr release cytotoxicity assay (CRA) for cell mediated immunity to the allogeneic tumor were done in parallel with same reagents used for both assays. Therefore, CRA was modified in which monolayer culture cells were used as target cells and MCA was devised in which the adherent surviving target cells after the interaction with effector cells were assessed by (51)Cr uptake (so called (51)Cr post-labeling assay...CPLA). Under standard conditions, CPLA detected a significant activity of weakly sensitized lymphocytes which was not detected by CRA. Reactivity of lymph node cells after the tumor transplantation was tested simultaneously in CRA, CPLA and the macrophage migration inhibition test (MIT). The data showed that CPLA detected immune reactivity for longer period than CRA, and as long as MIT. The lytic activity of lymph node cells 8 days after immunization was monitored by a long term CRA (40-hours' incubation). The cytotoxicity value for late 20 hr was remarkably higher than for early 20 hr. This in vitro activation of sensitized lymphocytes indicated a peak at 8 days and then declined, dissappeared at 14 days. When both assays were done under same condition, CPLA detected a moderate activity which was not detected by CRA. This result means that CPLA could detect immune responses which inhibit tumor cell growth without killing them. Both assays detected primarily a T lymphocyte-mediated activity. The CPLA also detected a weak non T-cell activity in anti-θ plus complement-treated alloimmune lymph node. en-copyright= kn-copyright= en-aut-name=TanakaNoriaki en-aut-sei=Tanaka en-aut-mei=Noriaki kn-aut-name=田中紀章 kn-aut-sei=田中 kn-aut-mei=紀章 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科教室 END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=5-6 article-no= start-page=713 end-page=720 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=19770630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Electrostatic transducer of apexcardiogram kn-title=静電型心尖拍動トランスデューサーの試作およびその有用性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Apexcardiogram by mean of non-invasive method may be more usefull provided that the technique for the transducer attachment was easier and reprodusable. In this report, some attempts on the transducer of apexcardiogram were described. A precordial electrode was placed about 1.5 mm above the chest wall of a patient. The electrostatic capacitance between the electrode and chest wall was transmitted to the CR oscillator and it modulated the frequency of pulse train generated with the oscillator. The signals were led to a conventional D.C. recorder or a cathode ray oscilloscope. There was no remarkable differences of the wave pattern between the apexcardiogram obtained by our method and by conventional piezoelectric transducer in normal cases. In some patients, however, some differences were observed especially in the depth of O-wave. The depth of O-wave increased in accordance with a rise in pressure of the transducer to the chest wall using the conventional method. The observations suggest that the A/E-O ratio might be varied depending on attaching pressure of the transducer to the chest wall. en-copyright= kn-copyright= en-aut-name=IshiiYoshihisa en-aut-sei=Ishii en-aut-mei=Yoshihisa kn-aut-name=石井義久 kn-aut-sei=石井 kn-aut-mei=義久 aut-affil-num=1 ORCID= en-aut-name=HiranoKazuhiro en-aut-sei=Hirano en-aut-mei=Kazuhiro kn-aut-name=平野和博 kn-aut-sei=平野 kn-aut-mei=和博 aut-affil-num=2 ORCID= en-aut-name=KawaiTatsuya en-aut-sei=Kawai en-aut-mei=Tatsuya kn-aut-name=河合辰哉 kn-aut-sei=河合 kn-aut-mei=辰哉 aut-affil-num=3 ORCID= en-aut-name=SaitoDaiji en-aut-sei=Saito en-aut-mei=Daiji kn-aut-name=斉藤大治 kn-aut-sei=斉藤 kn-aut-mei=大治 aut-affil-num=4 ORCID= en-aut-name=HaraokaShoichi en-aut-sei=Haraoka en-aut-mei=Shoichi kn-aut-name=原岡昭一 kn-aut-sei=原岡 kn-aut-mei=昭一 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第一内科教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一内科教室 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1259 end-page=1268 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hemolytic anemia in Rauscher virus-induced leukemia 2. Studies on function of reticuloendothelial system kn-title=Rauscher Virus性マウス白血病における溶血性貧血に関する研究 第2編 網内系機能の変動について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Using Rauscher leukemia virus, changes in the reticuloendothelial phagocytic activity were studied by a carbon clearance method and a heat-damaged (51)Cr-labelled red blood cell method. With the carbon clearance method, after virus infection, phagocytic activity was slightly increased in the early stages, but decreased gradually during later stages. With the (51)Cr-labelled RBC method, in the early stages, phagocytic activity of liver and spleen was slightly increased and during the later stages accelerated markedly in the liver in contrast to supressed activity in the spleen. It was suggested that an early increase of phagocytic activity observed by both methods was a response to viral infection. Significantly accelerated phagocytic activity seen in the liver during later stages with the (51)Cr method may have been related to the hemolytic anemia associated with Rauscher leukemia. en-copyright= kn-copyright= en-aut-name=MizukawaIchiro en-aut-sei=Mizukawa en-aut-mei=Ichiro kn-aut-name=水川市郎 kn-aut-sei=水川 kn-aut-mei=市郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 en-keyword=溶血性貧血 kn-keyword=溶血性貧血 en-keyword=網内系貪食機能 kn-keyword=網内系貪食機能 END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1117 end-page=1125 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells　as target cells　Part II. ADCC and erythrophagocytosis of peripheral leukocytes in patients with Sjogren's syndrome or rheumatoid arthritis kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第2編 Sjogren症候群ならびに慢性関節リウマチ患者白血球のADCC活性とErythrophagocytosis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent erythrophagocytosis (ADEP) of peripheral blood leukocytes in patients with Sjogren's syndrome (SjS) or rheumatoid arthritis (RA) were investigated using (51)Cr-labelled sheep red blood cells as target cells. The Lymphocytes (phagocyte depleted fraction, PDF) in healthy controls showed higher ADCC activities (58.6±9.9%) than the phagocyte-contaminated crude lymphocyte fraction (CLF) (49.1±10.9%). There was no statistically significant difference between the ADCC activities of PDF in patients with SjS (55.9±13.5%) or RA (58.8±9.6%) and those in healthy controls, although decreased ADCC activity was observed in some patients with SjS. ADCC activities of CLF in patients with SjS (61.2±14.3%) or RA (59.3±6.9%) were significantly higher than those of CLF in healthy controls. Decreased ADEP activity of monocytes in CLF was demonstrated in patients with SjS (17.9±9.4%) compared with healthy controls (37.2±10.6%), and increased ADCC activity of CLF in patients with SjS was dependent on decreased ADEP activity of contaminating monocytes. Decreased ADEP activity of monocytes in patients with RA was also suggested by these results. It was speculated that the decreased Fc-receptor function of peripheral blood monocytes, demonstrated in these patients, had originated from aberrant cell-mediated immunity in these diseases, since these Fe-receptor functions in patients with SjS did not correeate with the following conditions: associated diseases, erythrocyte sedimentation rate, titer of serum rheumatoid factor, lymphocyte count of peripheral blood, or treatment with anti-inflammatory drugs including prednisolone. en-copyright= kn-copyright= en-aut-name=IchikawaYukinobu en-aut-sei=Ichikawa en-aut-mei=Yukinobu kn-aut-name=市川幸延 kn-aut-sei=市川 kn-aut-mei=幸延 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第二内科学教室 en-keyword=antibody-dependent cell-mediated cytotoxicity kn-keyword=antibody-dependent cell-mediated cytotoxicity en-keyword=erthrophagocytosis kn-keyword=erthrophagocytosis en-keyword=sheep red blood cells kn-keyword=sheep red blood cells en-keyword=Sjogrens syndrome kn-keyword=Sjogrens syndrome en-keyword=rheumatoid arthritis kn-keyword=rheumatoid arthritis END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue=9-10 article-no= start-page=1101 end-page=1115 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells as target cells Part I. Fundamental studies with special reference to erythrophagocytosis kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第1編 Erythrophagocytosisを中心とした基礎的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral leukocytes was investigated using (51)Cr-labelled sheep red blood cells (SRBC) sensitized with rabbit anit-SRBC antibody as target cells. ADCC (lysis of target cells without erythrophagocytosis) was mediated by the lymphocytes. On the other hand, phagocytes including monocytes and granulocytes showed antibody-dependent erythrophagocytosis (ADEP). ADCC of the lymphocytes was augumented by the enrichment of Fc-receptor bearing or Nylon-wool column passing cells. Phagocytes showed rapid ADEP, and granulocytes released (51)Cr-radioactivity originating from target cells into the culture medium in a short time, whereas the monocytes released little radioactivity during the incubation period. The monocytes inhibited ADCC of the lymphocytes competitivelly through ADEP, since decreased ADCC activity of monocyte-contaminated crude lymphocyte fraction was recovered by elimination of contaminating monocytes. Antibody-independent erythrophagocytosis of SRBC was mediated by monocytes, but not by granulocytes. Both ADCC of lymphocytes and ADEP of phagocytes were dependent on IgG-antibody, IgM-antibody did not participate in these phenomena. ADEP of monocytes was detected at 10(-2)-10(-6) dilution of IgG-antibody fraction (original hemmagglutination titer; × 1600), and ADCC of lymphocytes and ADEP of granulocytes were detected at 10(-2)-10(-4) and 10(-2)-10(-3) dilution of this antibody fraction, respectively. Soluble cytotoxic factors were not released from lymphocytes, monocytes or granulocytes in this assay system, because no cytotoxic activity on (51)Cr-labelled human erythrocytes used as an adjacent cell was demonstrated by any leukocyte fraction in the presence of antibody-coated SRBC. en-copyright= kn-copyright= en-aut-name=IchikawaYukinobu en-aut-sei=Ichikawa en-aut-mei=Yukinobu kn-aut-name=市川幸延 kn-aut-sei=市川 kn-aut-mei=幸延 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=antibody-dependent cell-mediated kn-keyword=antibody-dependent cell-mediated en-keyword=cytotoxicity kn-keyword=cytotoxicity en-keyword=erythrophagocytosis kn-keyword=erythrophagocytosis en-keyword=sheep red blood cells kn-keyword=sheep red blood cells END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=1-2 article-no= start-page=9 end-page=15 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on antibody-anticancer drug conjugates Part 1. Complement dependent cytotoxicity and capping inhibition of antibody-Neocarzinostatin conjugate(NCS-immune IgG). kn-title=抗腫瘍抗体結合抗癌剤に関する研究 第1編 抗腫瘍抗体結合Neocarzinostatin(NCS-immune IgG)の補体依存細胞障害作用とcapping抑制効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the previous paper, we reported that Neocarzinostatin (NCS) could bind covalently to rabbit anti-tumor IgG (Immune IgG) without losing its pharmacological activity. In this study, the complement dependent cytotoxicity and capping inhibition of the conjugate were evaluated. When antibody activity was measured by (3)H-TdR uptake inhibition and (51)Cr release tests in the presence of human complement, the cytotoxicity of the conjugate was significantly reduced in contrast to the antibody binding activity tested by immunofluorescence. In addition, an anti-complement immunofluorescent test showed that NCS-immune IgG fixed less complement on NALL-1 cells than immune IgG. These results indicate that complement fixing sites of NCS-immune IgG are damaged during chemical conjugation. The capping experiment demonstrated that NCS-immune IgG interfered with the capping of antigens on the NALL-1 cell surface, and that the capping inhibiting activity was almost the same degree as that of free NCS. This unique advantage of NCS-immune IgG may be useful for inhibiting the immunological escape of tumor cells from antibody attack in vivo. en-copyright= kn-copyright= en-aut-name=SatoYuji en-aut-sei=Sato en-aut-mei=Yuji kn-aut-name=佐藤融司 kn-aut-sei=佐藤 kn-aut-mei=融司 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=Antibody-NCS conjugate kn-keyword=Antibody-NCS conjugate en-keyword=Complement dependent cytotoxicity kn-keyword=Complement dependent cytotoxicity en-keyword=Capping inhibition kn-keyword=Capping inhibition END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=9-10 article-no= start-page=961 end-page=972 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19841030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on diagnosis and treatment of lung cancer Part 2. Combination chemotherapy of advanced lung cancer with cyclophosphamide, vincristine, methotrexate and procarbazine kn-title=肺癌の診断と治療に関する研究 第2編 進展期肺癌症例におけるCyclophosphamide, Vincristine, Methotrexate, Procarbazine併用療法に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=One hundred and thirteen patients with advanced lung cancer were treated with a four-drug combination chemotherapy consisting of cyclophosphamide, vincristine, methotrexate and procarbazine. Of those, ninety three (80 men and 13 women) patients were evaluated. Of 41 patients with small cell carcinoma, 10 (24%) achieved complete response (CR) and 18 (44%) achieved partial response (PR). The median duration of response was 5 months. The median survival time from initiation of chemotherapy was 11.3 months for patients with limited disease and 8 months for those with extensive disease. Responders lived significantly longer than non-responders; median survival time was 14 months for complete responders, 10.3 months for partial responders, and 6 months for non-responders. Of 52 patients with non-small cell carcinoma, 2 achieved CR and 15 achieved PR: The overall response rate was 33% ; with a response rate of 30% (8/27) for adenocarcinoma, 30% (6/20) for squamous cell carcinoma, and 60% (3/5) for large cell carcinoma. The median survival time was 13 months for patients with stage Ⅲ, and 8 months for those with stage Ⅳ. Responders with non-small cell carcinoma displayed a tendency toward longer survival periods; the median survival time was 8.3 months for responders compared with 6 months for non-responders. Myelosuppressive toxicity remained within acceptable limits, with 8.8% incidence of leucocytepenia (1,000/cmm) and 6.7% incidence of thromobocytepenia (50,000/cmm) in repeated courses. These resuts indicate that this regimen is useful for the treatment of advanced lung cancer. en-copyright= kn-copyright= en-aut-name=MachidaKen-ichi en-aut-sei=Machida en-aut-mei=Ken-ichi kn-aut-name=町田健一 kn-aut-sei=町田 kn-aut-mei=健一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Lung cancer kn-keyword=Lung cancer en-keyword=Combination chemotherapy kn-keyword=Combination chemotherapy END start-ver=1.4 cd-journal=joma no-vol=96 cd-vols= no-issue=9-10 article-no= start-page=891 end-page=899 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=19841030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on biscoclaurine alkaloids in relation to radiation damage of hemopoietic tissue Ⅱ. Effect of cepharanthin on the recovery from radiation damage in mouse hemopoietic tissue kn-title=血液系細胞の放射線障害とビスコクラウリン型アルカロイドに関する研究 第2編 X線照射後のマウス造血組織に及ぼすセファランチンの効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=After a whole-body irradiation at a single dose of 500R, mice were injected intraperitoneally with 0.1mg of cepharanthin (CR)every day for 20days. Endogenous spleen colonies (CFU-S) were counted on Day 10, and the spleen and thymus weight was measured, peripheral leukocytes counted and hematocrit value determined for 30days after the irradiation. The number of CFU-S increased remarkably in CR injected mice compared with control mice. Decreased spleen weight by irradiation recovered in excess by Day 30. In CR administered mice, the spleen weight increased transitorily along with the proliferation of CFU-S. After irradiation, the recovery of thymus weight was delayed compared with the spleen. Therefore, the effect of CR administration was not clear. Peripheral leukocytes decreased in number successively essively up to Day 10 and recovered linearly from Day 20 through Day 30, especially in CR injected mice. The hematocrit value was reduced to a minimum on Day 10 and almost recovered by Day 20 in irradiated mice. CR had no effect on the hematocrit value. The effect of cepharanthin was discussed in relation to radiation damage of hemopoietic tissue. en-copyright= kn-copyright= en-aut-name=IidaSosuke en-aut-sei=Iida en-aut-mei=Sosuke kn-aut-name=飯田荘介 kn-aut-sei=飯田 kn-aut-mei=荘介 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 en-keyword=セファランチン kn-keyword=セファランチン en-keyword=放射線照射 kn-keyword=放射線照射 en-keyword=血液幹細胞 kn-keyword=血液幹細胞 en-keyword=臓器変化 kn-keyword=臓器変化 END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=1-2 article-no= start-page=15 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Induction chemotherapy for acute leukemia Part 2. Clinical trial of 5 drug combination chemotherapy (NCDVP, NCyclo-cDVP) for treatment of refractory acute leukemia kn-title=急性白血病寛解導入療法に関する研究 第2編 5剤併用療法(NCDVP療法, NCyclo-cDVP療法)による難治性急性白血病の寛解導入に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study, the clinical effectiveness of 5 drugs as combination chemotherapy, NCDVP (N:neocarzinostatin C: cytosine arabinoside D:daunorubicin V:vincrisitine P: prednisolone) and NCyclo-cDVP (Cyclo-c:cyclocytidine), for refractory acute leukemia was evaluated. Twenty patients (12 patients relapsed and 8 patients with the first induction failure) were entered in this study; 14 patients with acute non-lymphocytic leukemia (ANLL) and 6 patients with acute lymphocytic leukemia (ALL). Their ages ranged from 15 y.o. to 73 y.o. (median:39 y.o.) and the ratio of males to females was 15 to 5. Doses and administration methods were: N, 1,400u/m(2)/day, was administered by i.v. drip on day 3, 4, 5 and 6 and C, 40-80mg/m(2)/day, was administered by i.v. drip from day 1 to day 6. D, 20-25mg/m(2)/day, was administered by i.v. bolus on day 3 and 5 and V, 1.4mg/m(2)/day, i.v. bolus on day 2. P, 20-40mg/m(2)/day, was administered by i.v. drip or p.o. from day 1 to day 6. The treatment was repeated with at least a 7 days resting period. All patients were adequatedly treated and 9 of them, 45%, obtained complete remission (CR). One patient showed partial remission. On the basis of types of leukemia, 7 of 14 patients with ANLL, 50%, and 2 of 6 patients with ALL, 33%, achieved CR. In order to obtain CR, one or 2 courses of the treatment were required (median:1.4 courses) and the duration of CR ranged from 2 to 38 weeks (median:6 weeks). In peripheral blood, the nadir of white blood cell counts, neutrophils and thrombocytes was 600/cmm, 200/cmm and 24,000/cmm, respectively and the number of days to this nadir was 12 days for white blood cell counts, 11 days for neutrophils and 13 days for thrombocytes. The nadir of bone marrow nucleated cell counts was 7,800/cmm and the number of days to nadir was 10 days. As toxic manifestations, digestive side effects were marked, but were tolerable. Eight patients showed alopetia and 4 patients complained of pain in the parotid gland. One patient complained of a sense of numbness in the fingers. Toxic effects on liver function were recognized in 5 patients, one of whom died of fulminant hepatitis. The treatment of refractory acute leukemia is one of the major problems in the clinical management of leukemia. The results of the 5 drug combination chemotherapy presented here suggests its effectiveness in refractory acute leukemia in man. en-copyright= kn-copyright= en-aut-name=TokiokaMasaaki en-aut-sei=Tokioka en-aut-mei=Masaaki kn-aut-name=時岡正明 kn-aut-sei=時岡 kn-aut-mei=正明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 en-keyword=急性白血病 kn-keyword=急性白血病 en-keyword=化学療法 kn-keyword=化学療法 en-keyword=Neocarzinostatin kn-keyword=Neocarzinostatin END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=1-2 article-no= start-page=1 end-page=13 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Induction chemotherapy for acute leukemia Part 1. Clinical trial of NCMP two-step regimen for treatment of acute non-lymphocytic leukemia kn-title=急性白血病寛解導入療法に関する研究 第1編 NCMP two step療法による成人急性非リンパ性白血病の寛解導入に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The clinical effectiveness of a new protocol, the NCMP (N:neocarzinostatin C:cytosine arabinoside M:6- mercaptopurine P:prednisolone) two-step regimen was studied in 19 patients with acute non-lymphocytic leukemia (ANLL). All patients were previously untreated and ages ranged from 15 to 75 y.o. (median:54 y.o.). The ratio of male to female was 11 to 8. The doses and administration-routes of antileukemic agents were: N, 1,400u/m(2)/day, was administrated by i.v. drip on day 1, 2, 3 and 4 (Regimen 1) and N, 4,000u/m(2)/day, i.v. drip on day 1 and 2 (Regimen 2). C, 60-100mg/m(2)/day, was administered by i.v. drip and M, 60-100mg/m(2)/day, was given p.o. daily. P, 20-40mg/m(2)/day, was also given p.o. daily. As the first step, antileukemic agents were administrated until peripheral blood cell counts and bone marrow nucleated cell counts decreased to less than 1,200/cmm and 15,000/cmm, respectively. Treatment with or without N as the second step was started about 3-7 days after the first step treatment. Eighteen patients were adequately treated by the NCMP two-step regimen and 12 of 18 patients, 66.7%, obtained complete remission (CR). On the basis of the types of leukemia, CR was achieved in 11 of 16 patients with AML, 68.8%, and 1 of 2 patients with AMoL, 50%. The ratio of CR was higher in younger patients (less than 49 y.o.) than in elderly patients (more than 50 y.o.). These ratios were 85.7% and 54.5%, respectively. On the basis of therapeutic regimens, 8 of 11 patients, 72.7%, achieved CR by Regimen 1 and 4 of 7 patients, 57.1%, by Regimen 2. The duration of CR ranged from 1.0 to 39.5+M (median:11.5 M). The survivals from the diagnosis of leukemia were from 13 to 41+M (median:12.1 M) in all patients and from 4.2 to 41+M (median:16.4 M) in responders. As toxic manifestations, digestive side effects such as anorexia, 68.4%, and nausea and vomiting, 21.1%, were marked, but were tolerable. These results indicate that the NCMP two-step regimen is an effective induction chemotherapy for ANLL. en-copyright= kn-copyright= en-aut-name=TokiokaMasaaki en-aut-sei=Tokioka en-aut-mei=Masaaki kn-aut-name=時岡正明 kn-aut-sei=時岡 kn-aut-mei=正明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 en-keyword=急性白血病 kn-keyword=急性白血病 en-keyword=化学療法 kn-keyword=化学療法 en-keyword=Neocarzinostatin kn-keyword=Neocarzinostatin END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=11-12 article-no= start-page=1329 end-page=1340 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Reticuloendothelial Function in Patients with Systemic Lupus Erythematosus and Mice Part 2. Splenic Fc-Receptor Function (SFcF) in Patients with Systemic Lupus Erythematosus. kn-title=全身性エリテマトーデス及びマウスの網内系機能に関する研究 第二編 全身性エリトマトーデスの網内系機能(脾Fc受容体機能)低下に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The function of the reticuloendothelial system (RES) is important for deposition of circulating immune complexes (IC) in tissue. However, little is known about how IC located in tissues in patients with systemic lupus erythematosus (SLE). Splenic Fc-Receptor function (SFcF) was studied in 29 patients with SLE using IgG coated (51)Cr labelled autologous erythrocytes, and was compared with immunological abnormalities in sera and kidney. Impairment of SFcF was found in 21 of the patients (72.4%). There was a close inverse correlation between SFcF and CH50 (p<0.05). Patients with impaired SFcF tended to have a high level of circulating IC, but this was not statistically significant (p>0.05). A marked decrease in SFcF was found in patients with mesangial lupus nephritis and those with diffuse proliferative lupus nephritis. Patients with membranous lupus nephritis showed a mild SFcF abnormality. Decreased SFcF was reversible by steroid and/or plasma exchange (PE) therapy. Decreased SFcF could be improved after PE therapy alone. It was suggested that an abnormality in SFcF might force the progress of the diesase in patients with SLE. en-copyright= kn-copyright= en-aut-name=MiyakeHayashi en-aut-sei=Miyake en-aut-mei=Hayashi kn-aut-name=三宅速 kn-aut-sei=三宅 kn-aut-mei=速 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第三内科学教室 en-keyword=Reticuloendothelial system kn-keyword=Reticuloendothelial system en-keyword=Splenic Fc-receptor Function kn-keyword=Splenic Fc-receptor Function en-keyword=Systemic Lupus Erythematosus kn-keyword=Systemic Lupus Erythematosus END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=11-12 article-no= start-page=1299 end-page=1309 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Immunofunctional classification of non- Hodgkin's lymphoma (NHL) Part I. Correlation of the classification to chemotherapeutic response and survival kn-title=Non-Hodgkin's lymphomaの免疫機能的分類に関する研究 第1編 化学療法との関連性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To determine whether the analysis of lymphocyte surface markers provides clinically useful information, neoplastic cells from 51 adult patients with NHL were studied for surface markers and phagocytosis. The analysis revealed that NHL could be classified into four types according to the neoplastic cell type; T-cell, B-cell, Null-cell and true histiocyte types. On the basis of this immunofunctional classification, response to chemotherapy and survival of the patients were evaluated. The chemotherapeutic responsiveness rate (CR+PR rate) and complete remission (CR) rate of the T-cell type were shown to be the lowest in stage III and IV patients. Especially in stage IV, the CR rate of the T-cell type was statistically the lowest. Patients in both stages III and IV of the B-cell type survived the longest and those of the T-cell type, the shortest. In stage IV, the survival period of the T-cell type NHL patients was significantly shorter than those of the non-Burkitt B-cell type or Null-cell type. However, the difference between the survival of the B- and Null-cell types was not clear. These results indicate that the immunofunctional classification is some what useful for prediction of chemotherapeutic response and prognosis in NHL. en-copyright= kn-copyright= en-aut-name=IshikawaMorihiro en-aut-sei=Ishikawa en-aut-mei=Morihiro kn-aut-name=石川盛寛 kn-aut-sei=石川 kn-aut-mei=盛寛 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=Non-Hodgkin's lymphoma kn-keyword=Non-Hodgkin's lymphoma en-keyword=Immunofunctional classification kn-keyword=Immunofunctional classification en-keyword=Surface marker kn-keyword=Surface marker en-keyword=Chemotherapy kn-keyword=Chemotherapy END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=11-12 article-no= start-page=1159 end-page=1167 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immune Response against Skin Epidermal Cells of Allogeneic Skin Grafts in Rats: Detection of Lymphocyte-Dependent Antibodies kn-title=同種皮膚移植における皮膚上皮細胞に対する免疫能の変動 ―リンパ球依存性抗体(LDA)の測定― en-subtitle= kn-subtitle= en-abstract= kn-abstract=The mechanism of rejection of allogeneic organ grafts is not fully understood. The rejection of grafted skin is complicated as skin epidermal cells express skin specific antigens. Thus skin epidermal cells are suitable target cells for analyzing skin graft rejection in in vitro studies. In this study, (51)Cr labeled skin epidermal cells were prepared from rat tail skin by trypsin digestion. The susceptibility of skin epidermal cells to immune spleen cells in a lymphocyte mediated cytotoxicity assay and LDA in an antibody dependent cytotoxicity assay was examined in allogeneic skin grafts in rats. Skin epidermal cells were specifically killed by immune killer lymphocytes or LDA in combination with normal rat spleen cells. The optimal condition for assays were 10 to 12 hours of incubation and a 100:1 to 200:1 effector to target cell ratio. When the kinetics of LDA activity after allogeneic skin graft was examined, LDA appeared in the recipient serum just after the rejection and maintained activity for more than 40 days after the skin graft. To analyze the mechanism of graft rejection, it is resonable to use the same target cells as the graft itself in both cellular and humoral immunoassays. In this sense, skin epidermal cells are useful for the study of skin rejection. en-copyright= kn-copyright= en-aut-name=TanakaShinichiro en-aut-sei=Tanaka en-aut-mei=Shinichiro kn-aut-name=田中信一郎 kn-aut-sei=田中 kn-aut-mei=信一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科教室 en-keyword=皮膚上皮細胞 kn-keyword=皮膚上皮細胞 en-keyword=リンパ球依存性抗体 kn-keyword=リンパ球依存性抗体 en-keyword=拒絶反応 kn-keyword=拒絶反応 en-keyword=ラット皮膚移植 kn-keyword=ラット皮膚移植 END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=9-10 article-no= start-page=939 end-page=948 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the pathogenetic mechanism in autoimmune thyroid disorders Part 2 Antibody-dependent cell-mediated cytotoxicity to cultured thyroid cells kn-title=自己免疫性甲状腺疾患における甲状腺組織障害機序の研究 第2編 甲状腺上皮細胞を用いたADCCの検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the presence of decomplemented sera from 13 out of 14 patients with Hashimoto's disease and 6 out of 7 patients with Graves' disease, normal human leucocytes caused significant lysis of cultured thyroid cells, as estimated by the release of (51)Cr. Cytolysis was seen to proceed linearly during the first 9 hours, but ceased after that. Cytolysis was enhanced by the addition of complement at as low as the concentration which did not induce cytotoxic factor mediated lysis. A study of different effector cells mediating ADCC indicated that mononuclear and non-adherent cells were cytotoxic to cultured thyroid cells, but adherent cells were not effective in this system. The percentage of cytotoxicity correlated with the anti-microsomal antibody(MCHA) titer (r=0.63, p<0.01), but not with the anti-thyroglobulin antibody (TGHA) titer. Inhibition of ADCC by aggregated IgG suggested that immune complexes in the sera of patients with thyroid disorders might inhibit cell destruction by an ADCC mechanism. en-copyright= kn-copyright= en-aut-name=MiuraHiroshi en-aut-sei=Miura en-aut-mei=Hiroshi kn-aut-name=三浦洋 kn-aut-sei=三浦 kn-aut-mei=洋 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第三内科学教室 en-keyword=甲状腺上皮細胞 kn-keyword=甲状腺上皮細胞 en-keyword=ADCC kn-keyword=ADCC END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=9-10 article-no= start-page=913 end-page=927 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=STUDIES ON THE LONG-TERM SURVIVORS IN CHRONIC MYELOGENOUS LEUKFMIA AND THE CHEMOTHERAPY OF ITS BLASTIC PHASE Part 2 Studies on the Chemotherapy of Blastic Phase of CML kn-title=慢性骨髄性白血病の長期生存例ならびに急性転化時の治療に関する研究 第2編 慢性骨髄性白血病急性転化時の治療に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To improve the effect of chemotherapy of chronic myelogenous leukemic(CML) in blastic phase, the following clinical analysis were conducted. 1) The present study consisted of 53 adult patients with CML, who were registered to the 2nd Department of Medicine, Okayama University Hospital and diagnosed as blastic phase between from January 1970 to March 1980. There were 29 males and 24 females, and age ranged from 15 to 77 years (median: 41). 2) These 53 patients were divided into 4 groups. i) Group I-A (5 cases) was diagnosed as blastic crisis(BC) with hiatus leukemicus(HL) and initially treated with multicombination chemotherapy such as NCD, DMP or NCDP regimens (N: Neocarzinostatin, C: Cytosine arabinoside, D: Daunorubicin, M; 6-Mercaptopurine, P: Prednisolone). ii) Group I-B (16 cases) was diagnosed as BC with HL and initially treated with VP or VPM regimens (V: Vincristine, P: Prednisolone, M: 6-Mercaptopurine). iii) Group II-A (11 cases) was early diagnosed as BC without HL according to our established criteria, and initially treated with multicombination chemotherapy such as NCMP, DCMP, NDMP, NCDP or NCDVP. and, iv) Group II-B (21 cases) was early diagnosed as BC without HL and initially treated with VP, MP, or VPM. 3) Complete remission(CR) rate of each group was 20.0% in Group I-A, 56.2% in Group I-B, 27.3% in Group II-A and 47.6% in Group II-B. 4) Median survival and its surviving ranges after BC of each group were 3.6 months (1.8-4.5), 6.7 (1.0-24.2), 6.0 (2.6-22.8) and 13.0 (4.1-43.5). Median survival of CR-responders was 3.6, 10.0, 8.7 and 17.0 months, respectively. 5) The rate of one-year survivors of each group was 0%, 31.3% 9.1% and 52.4%. 6) In conclusion, the most ideal approach to prolongation of the survival period of CML patients after entering BC is i) to diagnose BC as early and accurate as possible, and ii) to treat these patients initially with rather mild regimens such as VP or VPM, instead of aggressive regimens with multicombination drugs. en-copyright= kn-copyright= en-aut-name=KitagawaNakayuki en-aut-sei=Kitagawa en-aut-mei=Nakayuki kn-aut-name=北川中行 kn-aut-sei=北川 kn-aut-mei=中行 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=慢性骨髄性白血病 kn-keyword=慢性骨髄性白血病 en-keyword=急性転化 kn-keyword=急性転化 en-keyword=早期診断・早期治療 kn-keyword=早期診断・早期治療 en-keyword=V(M)P療法 kn-keyword=V(M)P療法 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=7-8 article-no= start-page=661 end-page=676 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on combination chemotherapy for malignant lymphomaPart II. Combination chemotherapy in malignant lymphoma: Results of a long-term follow-up kn-title=悪性リンパ腫の化学療法に関する研究　第2編　進展期悪性リンパ腫に対する多剤併用療法の治療効果:長期観察による検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Results of combination chemotherapy for malignant lymphoma between 1973 and 1982 were analysed. In this series there were 17 patients with Hodgkin's disease (HD) and 109 patients with non-Hodgkin's lymphoma (NHL). None of the patients had prior chemotherapy. The two basic treatment programs in this series were BVCP or BCOP (combination of bleomycin, vincristine, cyclophosphafamide and prednisolone) and AVIP(combination of adriamycin, vincristine, ifosfamide and prednisolone). Of the 17 HD patients, 14 (82%) were effectively treated and achieved complete remission(CR). Six of the 14 patients relapsed between 15 and 65 months, while the remaining 8 patients have been disease-free between 8 and 126 months. The projected median CR duration was 65 months. No recurrent disease has occurred among 5 patients who were given AVIP as intensification therapy, suggesting the usefulness of adriamycin in the treatment of HD. Complete responders survived significantly longer than partial responders: 83% of the former survived 5 years, but none of the latter survived that long. Of the 109 patients with NHL, 25 patients had diffuse medium cell type histology(DM) and 43 patients had diffuse large cell type histology(DL). For DM, the CR rate was 64%, and the median response duration was 10 months. Of the 16 CRs, 9 have relapsed so far; however, the remaining 7 have been disease free for 12 to 95 months. For DL, the CR rate was 63% and the median remission duration was 43 months. Of the 27 CRs, 13 have relapsed so far, but the remaining 14 have been disease free for 13 to 115 months. The median survival time was 75 months for DM, and 23 months for DL. Complete responders lived significantly longer than partial or none-responders in DL. Prognostic factors were analysed in DM and DL patients. Among the chemotherapy effect, stage, constitutional symptoms, serum LDH, C-reactive protein (CRP), lymphocyte count of peripheral blood, and erythrocyte sedimentation rate(ESR), a CRP over 3+ and ESR over 30mm/hr were defined as poor prognostic factors for DM. Remission induction failure and CRP over 3+ were defined as poor prognostic factors for DL. Sixteen (6 HD and 10 NHL) of 72 complete responders (14 HD and 58 NHL) were disease free for at least 2 years after cessation of all treatment, suggesting that HD as well as NHL is curable by intensive combination chemotherapy. en-copyright= kn-copyright= en-aut-name=NishiharaRyuji en-aut-sei=Nishihara en-aut-mei=Ryuji kn-aut-name=西原龍司 kn-aut-sei=西原 kn-aut-mei=龍司 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=悪性リンパ腫 kn-keyword=悪性リンパ腫 en-keyword=多剤併用療法 kn-keyword=多剤併用療法 en-keyword=予後因子 kn-keyword=予後因子 END start-ver=1.4 cd-journal=joma no-vol=98 cd-vols= no-issue=3-4 article-no= start-page=255 end-page=264 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=19860430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Induction chemotherapy for acute leukemia Part 2. A comparative trial of a combination of daunorubicin and cytosine arabinoside and a combination of aclarubicin and cytosine arabinoside in acute non-lymphocytic leukemia kn-title=急性白血病寛解導入療法に関する研究 第2編 急性非リンパ性白血病寛解導入におけるdaunorubicin, cytosine arabinoside併用療法ならびにaclarubicin, cytosine arabinoside併用療法の比較検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A comparative trial of a combination of daunorubicin (DNR) and cytosine arabinoside (ara-C) (Regimen A) and a combination of aclarubicin (ACR) and ara-C (Regimen B) was performed. Sixteen patients with acute non-lymphocytic leukemia, previously untreated, included in this study. Five of 8 patients, 62.5%, obtained a complete remission (CR) with both Regimen A and B. The days until CR varied from 37 to 46 in Regimen A and from 22 to 56 in Regimen B. The total doses of DNR and ara-C in Regimen A were from 100 to 240mg and from 640 to 1,120mg, respectively. Those of ACR and ara-C in Regimen B were from 180 to 300mg and from 660 to 1,000mg, respectively. In a comparative study of hematological changes, toxic effects on peripheral white blood cells, platelets and nucleated cells in bone marrow tended to appear later in Regimen B than in Regimen A. Side effects on the digestive system such as nausea and vomiting and vascular pain were more frequently recognized in patients treated with Regimen B, although they were managed by symptomatic treatment. The present comparative study indicated that a new antileukemic anthracycline, ACR, has the same therapeutic activity against ANLL as DNR in combination with ara-C. en-copyright= kn-copyright= en-aut-name=HaraMasamichi en-aut-sei=Hara en-aut-mei=Masamichi kn-aut-name=原雅道 kn-aut-sei=原 kn-aut-mei=雅道 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=急性白血病寛解導入療法 kn-keyword=急性白血病寛解導入療法 en-keyword=aclarubicin kn-keyword=aclarubicin en-keyword=daunorubicin kn-keyword=daunorubicin en-keyword=cytosine arabinoside kn-keyword=cytosine arabinoside END start-ver=1.4 cd-journal=joma no-vol=98 cd-vols= no-issue=3-4 article-no= start-page=243 end-page=254 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=19860430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Induction chemotherapy for acute leukemia Part 1. Combination chemotherapy of aclarubicin and cytosine arabinoside in refractory acute non-lymphocytic leukemia kn-title=急性白血病寛解導入療法に関する研究 第1編 難治性成人急性非リンパ性白血病に対するaclarubicin, cytosine arabinoside併用療法効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Sixteen patients with refractory acute non-lymphcytic leukemia (ANLL) were treated with 2 different therapeutic regimens including aclarubicin (ACR) and cytosine arabinoside (ara-C). ACR, 45mg/㎡, was administered daily by intravenous rapid infusion from, day 1 to 3 in Regimen 1, and the same dose of ACR, divided into 2 doses, was administered every 12 hours by intravenous infusion from day 1 to 3 in Regimen 2. Ara-C, 80mg/㎡, divided into 2 doses, was given every 12 hours by intravenous infusion from day 1 to 7 in Regimen 1 and 2. Seven of 10 patients, 70%, obtained a complete remission (CR) with Regimen 1, and 1 of 6 patients, 16.7%, with Regimen 2. The days required for CR ranged from 22 to 55 days (median: 40 days). The duration of CR ranged from 2 to 13 months (median: 9.3 months), and the survival from the initiation of therapy was from 9 to 41.3 months (median 19.6 months) in responders. Side effects on the digestive system such as nausea and vomiting were seen in 14 of 16 patients, 87.5%, but they were all controllable. The combination of ACR and ara-C, particularly Regimen 1, was also found to be more effective in the clinical management of refractory ANLL than a NCDVP (neocarzinostatin, area-C, daunorubicin, vincristine and prednisolone) regimen. en-copyright= kn-copyright= en-aut-name=HaraMasamichi en-aut-sei=Hara en-aut-mei=Masamichi kn-aut-name=原雅道 kn-aut-sei=原 kn-aut-mei=雅道 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=急性白血病寛解導入療法 kn-keyword=急性白血病寛解導入療法 en-keyword=aclarubicin kn-keyword=aclarubicin en-keyword=cytosine arabinoside kn-keyword=cytosine arabinoside END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=3-4 article-no= start-page=289 end-page=299 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Induction Chemotherapy for Acute Leukemia Part 2. Clinical Evaluation of the Streptococcal Preparation, OK-432, in Induction Chemotherapy for Acute Nonlymphocytic Leukemia (ANLL) kn-title=急性白血病寛解導入療法に関する研究　第2編　急性白血病寛解導入療法における溶連菌製剤OK-432の評価 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study NCMP (neocarzinostatin, cytosine arabinoside, 6-mercaptopurine and prednisolone) two-step regimen with OK-432 was applied, to patients with ANLL in order to evaluate the clinical effects of the streptococcal preparation, OK-432. A total of 33 cases were randomized into two groups. Group A and group B were treated with NCMP two-step and NCMP two-step with OK-432, respectively. Group A consisted of 19 patients (16 AML and 3 AMoL) and group B consisted of 14 patients (13 AML and one AMMoL). All patients were previously untreated. The complete remission (CR) rate was 66.7% in group A and 64.3% in group B. However, patients in group B attained M(1) marrow and CR earlier than those in group A. Cytoreduction in peripheral blood and bone marrow was more rapidly obtained in group B than in group A. Normal hematopoiesis recovered more rapidly in group B than in group A. Cellular immunity (PPD) in group B tended to be maintained more than in group A. The CR rate in relapsed cases was higher in group B than in group A (44% vs 14%). Fever related to OK-432 was recognized in group B, but it was tolerable. OK-432 showed no prophylactic effects on infection. The simultaneous combination of OK-432 with chemotherapeutic agents might be expected to rapidly reduce leukemic cells, accelerate recovery of normal hematopoiesis and maintain cellular immunity during induction chemotherapy for ANLL. en-copyright= kn-copyright= en-aut-name=YorimitsuSeiichi en-aut-sei=Yorimitsu en-aut-mei=Seiichi kn-aut-name=依光聖一 kn-aut-sei=依光 kn-aut-mei=聖一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=急性白血病寛解導入療法 kn-keyword=急性白血病寛解導入療法 en-keyword=造血能賦活剤 kn-keyword=造血能賦活剤 en-keyword=溶連菌製剤OK-432 kn-keyword=溶連菌製剤OK-432 END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=11-12 article-no= start-page=1449 end-page=1460 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19871230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on monocytes in acute leukemia II. Monocyte chemiluminescence kn-title=急性白血病における単球の動態ならびに機能に関する研究　第2編　急性白血病における末梢単球化学発光の測定とその臨床的意義 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Levels of chemiluminescence (ChL) were measured in peripheral monocytes obtained from patients with acute leukemia. Items examined were base line (BL), peak level (PL), peak time (PT) and BL/PL ratio. Levels of monocyte ChL examined during different states of acute leukemia [before chemotherapy, state of complete remission (CR) and state of relapse] showed no significant differences as compared to those in healthy controls. However, prolongation of PT in untreated cases, elevation of BL and low BL/PL ratio in cases of CR and elevation of PL in relapsed cases tended to be observed. Four of 5 acute leukemia patients with CR, in which monocyte ChL was measured sequentially, showed an elevation of BL and PL before and/or during the early stage of relapse. Moderate inverse relations were observed between the percentage of leukemic cells in the marrow and monocyte BL and PL, respectively, and between the absolute counts of monocytes in the blood and monocyte BL and PL, respectively. These results suggest that there are two dynamic phases of monocyte ChL in acute leukemia; namely, BL and PL are rather high when the volume of leukemic cells is small, and BL and PL decrease as leukemic cells enlarge. The sequential measurement of monocyte ChL in the state of CR may aid in the early recognition of relapse. en-copyright= kn-copyright= en-aut-name=OhmotoEijiro en-aut-sei=Ohmoto en-aut-mei=Eijiro kn-aut-name=大本英次郎 kn-aut-sei=大本 kn-aut-mei=英次郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=acute leukemia kn-keyword=acute leukemia en-keyword=monocyte kn-keyword=monocyte en-keyword=monocyte chemiluminescence kn-keyword=monocyte chemiluminescence END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=9-10 article-no= start-page=873 end-page=883 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antibody dependent cell-mediated cytotoxicity against measles virus infected HeLa-cellsin in cord blood, infant blood and mother blood kn-title=麻疹ウイルス感染細胞に対する臍帯血および乳児血中のantibody dependent cell-mediated cytotoxicity抗体に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the role of antibody dependent cell-mediated cytotoxicity (ADCC) in the prevention of measles virus infection during the first year of life, ADCC activities in cord blood and peripheral blood of infants were examined by a (51)Cr-release assay method. Transmission of ADCC from the mother to the newborn was also evaluated.ADCC activities in the cord blood were demonstrated in 40 among 41 newborns whose mothers had a history of measles infection. A significant correlation in ADCC activities between cord blood and maternal blood was found (r=0.946). The antibody which mediates ADCC correlated with neither NT antibody nor HI antibody against measles virus in the cord blood. Thus, this antibody represented another group of antibody. HI antibody disappeared from the age of 6 months, while the antibody mediating ADCC remained until the age of 12 months. ADCC activities were detected even in the cord blood of newborns whose mothers were infected with measles 16 to 28 years ago. These results suggest that the antibody mediating ADCC against measles virus is transmitted from the mother to cord blood via the placenta. Furthermore, ADCC appears to play an important role in preventing the infection of infants between 6 to 12 months of age with measles virus. en-copyright= kn-copyright= en-aut-name=SugitaMakio en-aut-sei=Sugita en-aut-mei=Makio kn-aut-name=杉田真喜雄 kn-aut-sei=杉田 kn-aut-mei=真喜雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=麻疹感染防禦 kn-keyword=麻疹感染防禦 en-keyword=ADCC kn-keyword=ADCC en-keyword=臍帯血 kn-keyword=臍帯血 END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=5-6 article-no= start-page=621 end-page=631 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the treatment and management of malignant lymphoma Part 2. Combination chemotherapy for diffuse large-cell non-Hodgkin's lymphoma superiority of adriamycin combination kn-title=悪性リンパ腫の診断と治療に関する研究 第2編 びまん性大細胞型非ポジキンリンパ腫におけるAdriamycinを含む多剤併用療法の有用性に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A comparison was made between the therapeutic outcome of patients with diffuse large-cell non-Hodgkin's lymphoma treated with combination chemotherapy containing adriamycin (adriamycin regimen) and those treated with combination chemotherapy not containing adriamycin (non-adriamycin regimen). All the patients had no prior therapy, and there were no differences in any other background factors between the two treatment groups. Of 32 patients on the non-adriamycin regimen, 19 (59%) achieved complete response (CR) and 12 (38%) achieved partial response (PR). Of 20 patients on the adriamycin regimen, 14 (70%) achieved CR and 6 (30%) achieved PR. For those on the non-adriamycin regimen, the median duration of CR was 10 months, the projected 5-year relapse-free rate was 27%; the median survival time for all patients was 1 year 7 months; and the projected 5-year survival rate was 24%. For those on the adriamycin regimen, a median duration of CR was not reached, the projected 5-year relapse-free rate was 85%; the median survival time for all patients was 1 year 11 months; and the projected 5-year survival rate for all patients was 58%. These results show the superiority of combination chemotherapy containing adriamycin, indicating that adriamycin is a essential agent for the initial induction chemotherapy for the large-cell type of non-Hodgkin's lymphoma. en-copyright= kn-copyright= en-aut-name=YoshidaMitsuo en-aut-sei=Yoshida en-aut-mei=Mitsuo kn-aut-name=吉田光雄 kn-aut-sei=吉田 kn-aut-mei=光雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=非ホジキンリンパ腫 kn-keyword=非ホジキンリンパ腫 en-keyword=adriamycin kn-keyword=adriamycin en-keyword=多剤併用療法 kn-keyword=多剤併用療法 END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue=1 article-no= start-page=139 end-page=150 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=19791201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ozonation of Organic Substances from View Points of Wastewater Treatment en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this paper, ozonation of organic substances which are found in wastewater is discussed regarding following respects; (1)removal ratios of COD(Cr) and TOC, (2)removal properties, and (3)oxidation products. The main results are summarized as follows. (l)Ozonation quickly removes proteins and amino acids, but mildly polysaccharides and n-saturated carboxylic acids. (2)The initial removal of COD(Cr) or TOC is quite rapid, but, as the reaction continues, it slows down so drasti-cally that one may suspect that it has completed. (3)Starch and albumine are decomposed to the smaller molecular compounds. (4)The COD(Cr) /TOC ratio of each substance which is chosen in this study decreases. This means that ozonation results in the formation of oxygenated fragments of the original molecule-occurs by bond fission or partial oxidation. (5)Proteins are indispensable components in frothing because they are surface active. They are removed by not only oxidation but also foaming, however, the removal by foaming is not so large. en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=KawaraOsami kn-aut-sei=Kawara kn-aut-mei=Osami aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Civil Engineering END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue=1 article-no= start-page=17 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20021225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Nursing Care Workers' Concerns about Child Restraint System kn-title=チャイルドシートに関する看護職の意識調査 en-subtitle= kn-subtitle= en-abstract=This study surveyed nursing care workers (workers) understandings of regulations regarding the emergent transportation of childhood patients and child restraint systems (CRS). It also ascertained how they addressed issues involved in the transportation of children by car. Subjects were 725 workers at hospitals or health centers in Okayama City, who were sent an anonymous style questionnaire that was then retrieved by mail (recovery rate 72.2%). As the results, 84.6% of workers had never discussed childhood patient transportation during admission/discharge, with most not being involved in the transportation of children. Only 40.9% answered correctly to 5 questions regarding the exclusive provisions of CRS used to ascertain understanding of the regulations. There were two responses regarding "CRS application to fat children or those with mental restlessness": some encouraged the parents to make a decision and others took advices from doctors. A driver was held responsible for a car accident in Japan when a healthy child was transported without CRS. No case regarding the exclusive provisions has yet been reported. In cases of car accidents, drivers will from now surely be held responsible for the use of CRS. This survey provided nursing care workers for the opportunity to recognize the importance of CRS in the emergenty transportation of children. kn-abstract=本調査は患児の救急搬送時のことやCRS (child restraint system)における法規制の理解などに対する看護職の意識と子どもの車での搬送に関する問題への対応を知る目的で実施した｡岡山市内にある病院および保健所･保健センターに勤務している看護職725名(回収率72.2%)を対象に無記名アンケート方式で郵送法にて実施した｡その結果,入退院時の搬送については｢一度も話したことがない者｣は84.6%で,搬送に関してはほとんど関与していなかった｡CRSの除外規定に関する法的理解について5項目質問したが､正解者は40.9%であった｡その他"肥満児および精神的な不穏時のCRSの対応"として は保護者への判断を促す者と医師の判断を仰ぐ者との2通りの意見があった｡ わが国では健常児の非CRS使用時の搬送時に事故がおき運転者責任を問われたが,除外規定に関する判例ではなかった｡今後は交通事故の発生の場合には確実にCRSの問題は運転者の判断を問われることになる｡看護職としては子どもの搬送にCRSは関与していることを認識することへの示唆となるだろう｡ en-copyright= kn-copyright= en-aut-name=OnoKiyomi en-aut-sei=Ono en-aut-mei=Kiyomi kn-aut-name=小野清美 kn-aut-sei=小野 kn-aut-mei=清美 aut-affil-num=1 ORCID= en-aut-name=SumiyoshiKazuko en-aut-sei=Sumiyoshi en-aut-mei=Kazuko kn-aut-name=住吉和子 kn-aut-sei=住吉 kn-aut-mei=和子 aut-affil-num=2 ORCID= en-aut-name=OkazakiYuka en-aut-sei=Okazaki en-aut-mei=Yuka kn-aut-name=岡崎愉加 kn-aut-sei=岡崎 kn-aut-mei=愉加 aut-affil-num=3 ORCID= en-aut-name=OhtaTakeo en-aut-sei=Ohta en-aut-mei=Takeo kn-aut-name=太田武夫 kn-aut-sei=太田 kn-aut-mei=武夫 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=3 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=4 en-affil= kn-affil=岡山大学医学部保健学科 en-keyword=チャイルドシート (child restraint system) kn-keyword=チャイルドシート (child restraint system) en-keyword=看護職 (nursing care worker) kn-keyword=看護職 (nursing care worker) en-keyword=子どもの搬送 (child transportation) kn-keyword=子どもの搬送 (child transportation) END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=1-2 article-no= start-page=41 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20010327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alkenylation and Allylation of Aldehydes by Using an Ni/Cr/TDAE Redox System en-subtitle= kn-subtitle= en-abstract= kn-abstract=In 360 females, Bone mineral density (BMD) in lumbar vertebrae (L2-L4) was assessed by quantitative computed tomography (QCT), and the values obtained were compared with the frequency of vertebral transformation or fracture as assessed by lateral scan image (scanogram) by X-ray CT. A correlation was observed between the frequency of vertebral transformation (or fracture) and lumbar BMD values : BMD under 125 mg/cm(3) was observed over 90% of women with vertebral transformation, and BMD under 70mg/cm(3) was found about 50% of them. These results suggest that decrease in BMD in lumbar vertebrae leads to vertebral transformation or fracture. Thus, measurement of BMD by QCT would be very useful in predicting vertebral transformation or fractures. en-copyright= kn-copyright= en-aut-name=KuroboshiManabu en-aut-sei=Kuroboshi en-aut-mei=Manabu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaMuneaki en-aut-sei=Tanaka en-aut-mei=Muneaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KishimotoSuguru en-aut-sei=Kishimoto en-aut-mei=Suguru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GotoKentaro en-aut-sei=Goto en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TanakaHideo en-aut-sei=Tanaka en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Applied Chemistry affil-num=2 en-affil= kn-affil=Department of Applied Chemistry affil-num=3 en-affil= kn-affil=Department of Applied Chemistry affil-num=4 en-affil= kn-affil=Department of Applied Chemistry affil-num=5 en-affil= kn-affil=Department of Applied Chemistry END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=11-12 article-no= start-page=1311 end-page=1321 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=1991 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on chemotherapy for small cell lung cancer Part 2. Patterns of relapse in patients with small cell lung cancer receiving chemotherapy with or without Radiotherapy kn-title=肺小細胞癌の化学療法に関する研究　第2編　肺小細胞癌完全寛解例における再発様式の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Between 1976 and 1987, a total of 180 patients with small cell lung cancer who were entered into three protocol studies of an intensive chemotherapy with or without radiotherapy was fully evaluated for tumor response and survival. Of them, 74 patients achieved a complete response (CR) : 47 out of 91 patients with limited disease (LD) and 27 out of 89 patients with extensive disease (ED). The author analysed the pattern of relapse among the 74 patients in an attempt to clarify the role of chest irradiation and/or prophylactic cranial irradiation. Of 47 patients with LD achieving a CR, 20 patients (43%) developed initial relapse in the chest and seven (15%) developed it in the brain. Of the 27 patients with extensive disease, the chest was also the most frequent site of initial relapse (44%) followed by the brain (19%). In patients with LD receiving chemotherapy plus chest irradiation, the rate of initial relapse in the chest and the cumulative actuarial probability for initial chest relapse 2 years later were 29% and 37%, respectively. These figures were siginificantly lower than the rate of 69% and the probability of 69% for patients receiving chemotherapy alone (p<0.05). The patient survival was improved by the addition of chest irradiation substantially, but not significantly. However, long-term disease-free survivors predominated in the group of patients receiving chemotherapy plus chest irradiation. Prophylactic cranial irradiation exerted no significant advantage for prolonging survival. However, it showed a trend for reducing the brain relapse in complete responders. These findings indicate that chest irradiation concomitant with intensive chemotherapy may be effective for prolonging suvival in patients with LD through preventing relapse from the chest. en-copyright= kn-copyright= en-aut-name=YamashitaHidetoshi en-aut-sei=Yamashita en-aut-mei=Hidetoshi kn-aut-name=山下英敏 kn-aut-sei=山下 kn-aut-mei=英敏 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Pattern of relapse kn-keyword=Pattern of relapse en-keyword=Small cell lung cancer kn-keyword=Small cell lung cancer en-keyword=chest irradiation kn-keyword=chest irradiation en-keyword=prophylactic cranial irradiation kn-keyword=prophylactic cranial irradiation END